Aspectos da dieta, atividade, uso do hábitat e esforço reprodutivo de Hylodes nasus (Anura: Hylodidae) no Parque Nacional da Tijuca, Rio de Janeiro, RJ

Em uma das maiores florestas urbanas do mundo, o Parque Nacional da Tijuca no Rio de Janeiro, se situa uma espécie do gênero Hylodes: Hylodes nasus. Sobre esta espécie existem poucos estudos, os principais relatam sobre seu comportamento territorialista, suas vocalizações e sobre seu giríno. Neste trabalho, nós estudamos como se dá a atividade, o uso do hábitat, o esforço reprodutivo e a dieta de H. nasus. Esta espécie é endêmica do Maciço da Tijuca e está restrita a riachos, o qual tem se provado ser bastante relacionado ao seu modo de reprodução. Os dados revelaram que a espécie tem uma atividade essencialmente diurna que está muita ligada ao fotoperíodo e que essa atividade tem uma tendência a diminuição no período do meio do dia. Alguns trabalhos tem atribuído a diminuição da atividade de outras espécies à redução da intensidade luminosa alcançando seus sítios de vocalização, devido à posição do sol. A espécie, quando ativa, frequentemente permanece sobre pedras e perto de quedas dágua, que parecem ter um papel importante na sua atividade de vocalização. Verificamos uma mudança no uso dos micro-habitats entre o período diurno e o período noturno. Quanto à dieta os dados indicaram que esta é uma espécie que se alimenta predominantemente de um pequeno grupo de insetos (Diptera, Formicidae, Coleoptera e Hymenoptera). Ainda assim foi encontrado um grande espectro de presas presentes nos seus estômagos, principalmente de presas abundantes em riachos da Mata Atlântica. Tal fato pode indicar um comportamento mais oportunista e generalista por parte da espécie. O esforço reprodutivo empregado pelas fêmeas da espécie tendeu a ser maior no número de ovócitos do que no tamanho destes quando comparamos estes parâmetros às outras espécies de Hylodes. O tamanho e massa das fêmeas também tiveram uma relação positiva com o número respectivo de ovócitos encontrados nelas

Produção in vitro, análise fitoquímica e avaliação da atividade antineoplásica de metabólitos de Hovenia dulcis Thunb. (Rhamnaceae) obtidos por diferentes estratégias biotecnológicas

Hovenia dulcis Thunberg, natural da Ásia Oriental, é cultivada no Brasil onde é conhecida como uva-do-japão. A espécie possui várias indicações na medicina popular e alguns estudos apontam o seu potencial antineoplásico, tripanocida e hepatoprotetor. Metabólitos secundários são substâncias não essenciais para a sobrevivência celular, mas que fornecem vantagens adaptativas aos vegetais, sendo atribuído, para algumas delas, atividades biológicas importantes. Substâncias de interesse medicinal têm sido obtidas por técnicas da cultura de tecidos vegetais, como a calogênese e a cultura de células em suspensão, que permitem a síntese de matéria-prima de forma contínua e homogênea, independentemente de fatores ambientais e sazonais. O presente estudo objetivou o estabelecimento de culturas in vitro de H. dulcis, visando à produção de metabólitos de interesse, com vistas à avaliação do seu potencial antineoplásico sobre células K562. Foram testados protocolos para o estabelecimento de diferentes sistemas, como culturas de calos, de células em suspensão (CCS) e compact callus clusters (CCC) e ainda a avaliação do uso de elicitores na otimização de metabólitos produzidos in vitro. Foi verificado que a adição dos fitorreguladores KIN e TDZ, substituindo o BAP, não foi capaz de induzir a formação de calos friáveis, bem como a manutenção das culturas em ausência de luz. O uso do nitrato de prata promoveu a friabilidade de calos em todas as concentrações testadas, considerando-se 2,0 mg.L-1 a melhor concentração. Foram alcançadas taxas de 100% de formação de CCS tanto na presença, quanto em ausência de AgNO3. O maior acúmulo de biomassa foi verificado na concentração mais baixa de PIC (0,625 mg.L-1). A análise dos espectros de RMN indicou a presença de (+)-dihidromiricetina, (+)-galocatequina, hovenitina II, hovenosideo G, hodulosideo III, hodulosideo IV, hodulosideo I e hovenidulciosideo B1 nas culturas de calos friáveis. No estabelecimento de culturas CCC, observou-se a formação de calos compactos verdes em todas as concentrações de ANA testadas. O aumento da velocidade de rotação para 135 rpm aumentou a dispersão das células com consequente formação dos agregados celulares desejados. A seleção de linhagens celulares demonstrou ser um método eficiente na uniformização do tamanho desses agregados e tal uniformidade se manteve estável por mais de cinco subcultivos em 100% das culturas. Uma fração rica em saponinas foi obtida a partir dos agregados celulares, correspondendo a 1,46% da massa seca. A análise por RMN sugeriu a presença das saponinas Hovenosideo G e dos hovenidulciosideos A2 e B2. O uso de elicitores em cultura de calos mostrou-se adequado à produção de metabólitos secundários, sem alterações morfológicas nos mesmos. A elicitação alterou o perfil cromatográfico analisado por HPLC. Na elicitação com 5,0 mg.L-1 de extrato de levedura foi verificado um aumento de quase três vezes (12,280 3,396 equivalentes de quercetina/mg de extrato) na síntese de flavonoides. Finalmente, os estudos de ação antitumoral in vitro demonstraram citotoxicidade dos extratos de calos não elicitados de H. dulcis sobre linhagem de leucemia mieloide crônica (IC50 de 74,05 μg.mL-1.) e inibição do crescimento de tais células (K562), sugerindo o potencial antineoplásico para um produto biotecnológio (calo) desta espécie.

A mechanism for the link between solar-irradiance variations and regional precipitation

Although the mechanisms of climatic fluctuations are not completely understood, changes in global solar irradiance show a link with regional precipitation. A proposed mechanism for this linkage begins with absorption of varying amounts of solar energy by tropical oceans, which may aid in development of ocean temperature anomalies. These anomalies are then transported by major ocean currents to locations where the stored energy is released into the atmosphere, altering pressure and moisture patterns that can ultimately affect regional precipitation. Correlation coefficients between annual averages of monthly differences in empirically modeled solar-irradiance variations and annual state-divisional precipitation values in the United States for 1950 to 1988 were computed with lag times of 0 to 7 years. The highest correlations (R=0.65) occur in the Pacific Northwest with a lag time of 4 years, which is about equal to the travel time of water within the Pacific Gyre from the western tropical Pacific Ocean to the Gulf of Alaska. With positive correlations, droughts coincide with periods of negative irradiance differences (dry, high-pressure development), and wet periods coincide with periods of positive differences (moist, low-pressure development).

Solar-cycle modulations of ENSO: a possible source of climatic change

An association between long-term changes in the solar cycle and the frequency of El Niño events has been identified in historical records of El Niño and sunspot number. Although no known mechanism can explain the apparent relationship, the association is strong. A possible coupling between the sun and the ocean's mixed layer, involving ENSO, is worthy of further study.

Radiocarbon record of solar variability and Holocene climatic change in coastal southern California

EXTRACT (SEE PDF FOR FULL ABSTRACT): Pollen analysis and 5 radiocarbon dates for a 687-cm core provide a detailed chronology of environmental change for San Joaquin Marsh at the head of Newport Bay, Orange County, California. Sediment deposition kept pace with sea level rise during the mid-Holocene, but after 4500 years BP, sea water regularly reached the coring site, and salt marsh was the local vegetation. Brief periods of dominance by fresh-water vegetation 3800, 2800, 2300 and after 560 years BP correlate global cooling events and (except the 3800-year BP event) with carbon-14 production anomalies. The coincidence of climate change and carbon-14 anomalies support a causal connection with solar variability, but regardless of the causal mechanism(s) the delta-carbon-14 curves provide a chronology for global, high-frequency climatic change comparable to that of Milankovitch cyclicity for longer time scales.

Evidence from the Pacific Northwest for solar modulation of climate and of northern ecosystems

EXTRACT (SEE PDF FOR FULL ABSTRACT): The 250-year net annual snow accumulation, or mass balance, time series derived from the Mt. Logan (Yukon) ice core has been spectrally analyzed and is found to contain a nominal 11-year waveform. The stable isotope time series contains a significant amount of power between 9 and 13 years, although this record is evidently not a straightforward proxy for air temperatures. The signal in the mass balance time series exhibits a close relationship with the sunspot cycle waveform and is, therefore, assumed to be related to it. Waveforms showing a high correlation with the solar cycle are found in other climate data in the region. ... Taken collectively, the data point to a link between solar variability, atmospheric variability, climate, and selected ecological dynamics in the Pacific Northwest, but other data, not presented, indicate these relationships may hold elsewhere. So far, the evidence is empirical; complete details of the physical mechanisms involved have yet to be synthesized in a satisfactory way.

Determining the role of linear and nonlinear interactions in records of climate change: possible solar influences in annual to century-scale records

Time series analysis methods have traditionally helped in identifying the role of various forcing mechanisms in influencing climate change. A challenge to understanding decadal and century-scale climate change has been that the linkages between climate changes and potential forcing mechanisms such as solar variability are often uncertain. However, most studies have focused on the role of climate forcing and climate response within a strictly linear framework. Nonlinear time series analysis procedures provide the opportunity to analyze the role of climate forcing and climate responses between different time scales of climate change. An example is provided by the possible nonlinear response of paleo-ENSO-scale climate changes as identified from coral records to forcing by the solar cycle at longer time scales.

Climate forcing by changing solar radiation

By how much does changing radiation from the Sun influence Earth's climate compared with other natural and anthropogenic processes? Answering this question is necessary for making policy regarding anthropogenic global change, which must be detected against natural climate variability. Current knowledge of the amplitudes and time scales of solar radiative output variability available from contemporary solar monitoring and historical reconstructions can help specify climate forcing by changing radiation over multiple time scales.

Potential solar radiation at H.J. Andrews Experimental Forest, Oregon

EXTRACT (SEE PDF FOR FULL ABSTRACT): Potential (clear-sky) radiation receipt is modeled for the slopes of the H.J. Andrews Experimental Forest Long-Term Ecological Research site in the foothills of the southern Cascade mountains of central Oregon. The modeling method developed by Williams is selected and applied to the forest area for the times of the solstices and equinox as well as mid-month times in January, February, April, and May in order to completely characterize the seasonal change of potential radiation at the location. ... It seems that Lookout Creek approximately divides the Andrews Forest into an area of relatively high potential radiation to the north of the creek and relatively lower potential radiation values to the south of the creek. Potential radiation values seem to be associated with the Andrews GIS data layers of debris flows and predominant tree species zones.

Addendum I: Paleoclimate and the Solar-Insolation/Tidal-Resonance Climate Model

Recent papers provide detailed analyses of more than 40 high-resolution time series culled from the extensive paleoclimate literature that appear to define cyclical elements of the Solar-Insolation/Tidal-Resonance Climate Model. This model was earlier referred to as the Milankovitch/Pettersson Climatic Theory. This paper provides comparable analyses of an additional 20 or so, evidently supportive, climate and volcanic time series. The tree-ring, historical, pollen, cultural, time-frequency, and hydrologic records range in length from 400 to 90,000 years and spatially from Alaska to Tierra del Fuego.

Storage characteristics of solar-dried Indian mackerel

Fresh Rastrelliger kanagurta (Indian mackerel) was thoroughly washed, eviscerated, cleaned and salted overnight with dry salt (fish : salt :: 5:1). Salted mackerel was dried in solar drier and on cement floor under direct sun for three days. The temperature inside the drier was 948°C higher than the ambient temperature. The rate of drying was higher in solar drier than on cement floor. The dried fish packed in 300-gauge polythene bags was subjected to biochemical, microbiological and organoleptic evaluation at regular intervals to assess the storage life. The overall quality of fish dried in solar drier was better than that of the fish dried on cement floor under direct sun.

Quality assessment of rotating and solar tunnel dried marine fish product

Studies were conducted to evaluate the quality aspects of marine dried fish i.e. silver jew fish (Johnius argentatus), Bombay duck (Harpodon nehereus) and ribbon fish (Trichiums haumela) products produced in rotating and solar tunnel dryers. On the basis of organoleptic characteristics such as colour, odour, texture, broken pieces, insect infestation and overall quality, four member panels of experts evaluated the quality of the dried products obtained from both rotating and solar tunnel dryers and all the products were found in acceptable quality. Reconstitutions properties of samples were in the range of 51.05 to 98.75% for the dried fish produced in rotating dryer, while 24.64 to 76.76% for dried fish produced in solar tunnel dryer. The highest reconstitution rate was found in dried silver jew fish and lowest in ribbon fish produced in rotating dryer. On the other hand, the highest reconstitution was observed in dried silver jew fish and lowest in dried Bombay duck produced in solar tunnel dryer. Proximate composition such as moisture, crude protein, lipid and ash content of the dried fish muscles produced in rotating dryer ranged from 16.36% to 19.1%, 62.35% to 67.37%, 6.37% to 10.75% and 7.00% to 8.05%, respectively and in solar tunnel dried fish products, they were in the range of 14.05% to 19.71%,57.64% to 69. 21%,6.92% to 15.40%and 7.69% to 8.80 %, respectively. The TVBN values of dried fish products obtained from rotating dryer were in the range of 15.02 to 19.05 mg/100g, while in solar tunnel dried fish products, the values were in the range of 15.46 to 19.21 mg/100g. The results of the studies indicated that dried fish produced from both rotating and solar tunnel drier were acceptable quality in terms of organoleptic and food quality aspects.