The 17th Annual Eric E. Williams Memorial Lecture

This flyer promotes an event for The 17th Annual Eric E.Williams Memorial Lecture, featuring the Honorable Dr. Ralph Everard Gonsalves, Prime Minister of Saint Vincent and the Grenadines. The event was held on October 23, 2015.

The 17th Annual Eric E. Williams Memorial Lecture- The US-Cuba Accord: How the Caribbean Paved the Way

This flyer promotes an event for The 17th Annual Eric E.Williams Memorial Lecture, featuring the Honorable Dr. Ralph Everard Gonsalves, Prime Minister of Saint Vincent and the Grenadines. The event was held on October 23, 2015 at the Wertheim Performing Arts Center, Florida International University.

Expression and purification of tau protein and its frontotemporal dementia variants using a cleavable histidine tag

Recombinant tau protein is widely used to study the biochemical, cellular and pathological aspects of tauopathies, including Alzheimer's disease and frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTPD-17). Pure tau in high yield is a requirement for in��vitro evaluation of the protein's physiological and toxic functions. However, the preparation of recombinant tau is complicated by the protein's propensity to aggregate and form truncation products, necessitating the use of multiple, time-consuming purification methods. In this study, we investigated parameters that influence the expression of wild type and FTPD-17 pathogenic tau, in an attempt to identify ways to maximise expression yield. Here, we report on the influence of the choice of host strain, induction temperature, duration of induction, and media supplementation with glucose on tau expression in Escherichia coli. We also describe a straightforward process to purify the expressed tau proteins using immobilised metal affinity chromatography, with favourable yields over previous reports. An advantage of the described method is that it enables high yield production of functional oligomeric and monomeric tau, both of which can be used to study the biochemical, physiological and toxic properties of the protein.

Gravidez e Doen��a de Wilson: Revis��o de 3 Casos Cl��nicos

A doen��a de Wilson �� uma doen��a heredit��ria autoss��mica recessiva que afecta o gene ATP7B, localizado no cromossoma 13. Caracteriza-se por uma altera����o do transporte transmembranar do cobre, acumulando-se este no f��gado e noutros ��rg��os, principalmente c��rebro, rins e c��rnea. A preval��ncia mundial �� de aproximadamente 30 casos por milh��o de habitantes. Cerca de 60 a 70% dos casos s��o diagnosticados entre os 8 e os 20 anos de idade. Clinicamente, apresenta-se como doen��a hep��tica, neurol��gica ou psiqui��trica. O seu diagn��stico �� baseado num alto ��ndice de suspei����o e na combina����o de achados cl��nicos e laboratoriais. O exame gold standard para o diagn��stico �� a bi��psia hep��tica com doseamento do cobre. O tratamento consiste na terap��utica farmacol��gica permanente ou no transplante hep��tico. Existem actualmente tr��s f��rmacos dispon��veis: a penicilamina e a trientina (agentes quelantes) e o zinco. O tetratiomolibdato �� um agente quelante ainda em investiga����o. A capacidade reprodutiva das mulheres com doen��a de Wilson tem melhorado com o aumento da e���c��cia destas terap��uticas e, como tal, a gravidez torna-se mais frequente. Esta parece n��o afectar o curso da doen��a e o principal problema que se coloca �� a escolha da terap��utica mais adequada durante a gesta����o, dado que a seguran��a destes f��rmacos na gravidez n��o est�� garantida. Os autores descrevem 3 casos de gr��vidas com doen��a de Wilson, vigiadas na sua institui����o, e discutem a controv��rsia em torno do uso destes f��rmacos no tratamento da doen��a durante a gravidez e amamenta����o.

BM: Efectos de una pol��tica cambiaria de tipo fijo frente a una pol��tica cambiaria flexible sobre la inflaci��n en Colombia : 1977 -1992

Frente a la pol��tica de apertura comercial que se viene desarrollando desde 1990 en Colombia, el manejo econ��mico ha tenido como prop��sito fundamental lograr integrar la econom��a nacional al exterior. Una de las herramientas de pol��tica econ��mica m��s importantes es la TASA DE CAMBIO; frente a ��sta, es preciso adoptar una posici��n y conocer el manejo que se le ha dado a esta variable durante los ��ltimos veinte a��os. A partir de 1967, la pol��tica cambiar��a colombiana se bas�� en un sistema de devaluaci��n gota a gota (Crawling Peg). Esta pol��tica continu�� hasta 1991, cuando la Junta Monetaria cambi�� la devaluaci��n gota a gota, por el sistema de Certificado de Cambio con descuento. En enero de 1994, la nueva Junta Directiva del Banco de la Rep��blica elimin�� el sistema de certificado de cambio con descuento e introdujo el sistema de Bandas Cambiar��as

La pol��tica del Presidente Wilson en Am��rica Central: la lucha contra la inestabilidad econ��mica

Introducci��n Los planificadores de�� la pol��tica exterior de Estados Unidos siempre le han presentado gran inter��s a la estrat��gica regi��n centroamericana. Los europeos hab��an invertido intensamente en la regi��n y Estados Unidos comprend��a que, a menos que asumiera la responsabilidad por la estabilidad econ��mica y pol��tica de los estados centroamericanos, los gobiernos de Europa ignorar��an la Doctrina Monroe cuando se tratase e proteger los intereses del exterior, Estados Unidos deber�� mantener el orden, per la abierta intervenci��n militar era costosa y era generadora de mala voluntad en todo el hemisferio. En�� consecuencia, el gobierno de Wilson busc�� otros medios para promover la estabilidad y la hegemon��a estadunidense. La Primera Guerra Mundial produjo una coyuntura de especiales oportunidades, as�� como de particulares riesgos.

Caracteriza����o de Fusarium decemcellulare isolado de guaranazeiro (Paullinia cupana var. sorbilis) por meio de ERIC-PCR.

A presente pesquisa visa analisar a diversidade gen��tica De F. decemcellulare isolado de mudas e plantas adultas de guaranazeiro com sintomas de superbrotamento, hipertrofia floral ou galhas por meio do marcador molecular ERIC-PCR.

Endangerment and Likeability of Wildlife Species: How Important are they for Payments Proposed for Conservation?

This paper examines empirically the relative influence of the degree of endangerment of wildlife species and their stated likeability on individuals' allocation of funds for their conservation. To do this, it utilises data obtained from the IUCN Red List, and likeability and fund allocation data obtained from two serial surveys of a sample of the Australian public who were requested to assess 24 Australian wildlife species from three animal classes: mammals, birds and reptiles. Between the first and second survey, respondents were provided with extra information about the focal species. This information resulted in the dominance of endangerment as the major influence on the allocation of funding of respondents for the conservation of the focal wildlife species. Our results throw doubts on the proposition in the literature that the likeability of species is the dominant influence on willingness to pay for conservation of wildlife species. Furthermore, because the public's allocation of fund for conserving wildlife species seems to be more sensitive to information about the conservation status of species than to factors influencing their likeability, greater attention to providing accurate information about the former than the latter seems justified. Keywords: Conservation of wildlife species; Contingent valuation; Endangerment of species; Likeability of species; Willingness to pay