Avaliação da biodegradação de tolueno por fungos isolados de ambientes associados ao petróleo

Spills can ocurr during oil productive chain and contaminate various environments due to the toxicity of monoaromatics hidrocarbons. Toluene stands out for being agressive to the nervous sistem and teratogenic, with high mobility and solubility in water, which facilitates environmental impact. Studies show that fungi are potential aromatic compounds assimilators, encouraging new researches about its use on the recovery of contaminated sites. This study aimed to select and characterize fungus with potential for biorremediation of toluene. 50 fungi were selected of the Collection of Microorganisms of Interest for Oil Gas and Biofuels, of UNESP Rio Claro, all of which were isolated from sites contaminated with monoaromatic hydrocarbons. Two trials were realized to select the microorganism with greater potential. The first test evaluated fungal growth under toluene saturated atmosphere. 24 fungi were chosen because its greater biomass production to participate in the next trial, the degradation in plates test, where the blue redox agente, DCPIP, indicates the degradation reaction, turning colorless. From this teste was possible to select one isolate which showed higher growth and stronger medium discoloration as the microorganism with the greatest potential to assimilate toluene. The Trichoderma cf. koningii had its potential evaluated through gas cromatography. The experiment proved the efficiency of the methodology, with positives results from the method validation and the effectiveness demonstrated of the LA-PHA-PACK bottles to prevent the volatilization of toluene during the 21 days of experiment. Being reliable its use for monitoring toluene decay associating it with degradation. This results are important because there aren't many methodologies and vials efficient to the purpose of this work. In the present study the degradation rates demonstrated no significant decay of the concentration of hydrocarbon. That may be related to the...

Avaliação do conhecimento dos profissionais de saúde sobre o programa de cessação do tabagismo

Pós-graduação em Enfermagem - FMB

Validação de protocolo sensorial para avaliação de carne bovina

Beef quality control, particularly its sensory characteristics, is an important factor for producers and retailers in order to satisfy consumer’s choices. Sensory analysis is an important tool to evaluate attributes that cannot be measured by easily available instrumental techniques, as well as texture – tenderness and juiciness – whose human perception is more complete, through trained panels. The aim of this study was evaluate the use of a beef sensory analysis protocol in three different laboratories. Six commercial samples of different brands of aged beef and 14 samples from crossbred animals (Bonsmara × Nelore - 7 and Canchim × Nelore - 7), aged during 14 days were analyzed. The samples were distributed to each participant laboratory, where 7 to 12 panelists were trained. A sheet containing a 9 cm non-structured scale with 14 attributes was used. The attributes were brown colour (CMAR); aponevrosis (PNAP); hydration degree (GH); characteristic beef aroma (SCCB); salty taste (SS); liver flavour (SF); fat flavour (SG); metallic flavour (SM); tenderness (MZ); juiciness (SL); fibrosity (FBS) and liver texture (SF). Obtained data was analyzed using analysis of variance and principal component analysis (PCA). The results showed that there was no interaction between samples and laboratories, indicating that all of them responded in a similar manner in relation to the samples, except PNAP attribute, which was expected as meat is very non-uniform normally. Samples were well differentiated in all laboratories as it could be observed in PCA graphs. With proper training it is possible to use a standard protocol for beef sensory analysis.

Validação do roteiro de entrevista de Habilidades Sociais Educativas Parentais (RE-HSE-P)

The study of the parental educative social skills is extremely important for promoting the development and learning of children within interactive situations. The objective of this study was to assess reliability and validity of the Parental Educative Social Skills Interview Script (RE-HSE-P). It was proceeded: a) the test-retest reliability assessment, with a sample of 41 participants assessed in two distinct moments, with an interval of two months and the Cronbach's alpha was calculated; b) 213 parents of preschoolers (n =114) and school age children (n = 98) evaluated their children,were assessed for the construct and discrimination validity studies, differentiated in groups: a) demand for psychological intervention x community children without problems and b) children with behavior problems x children without behavior problems. The results showed satisfactory values of internal consistency and validity; besides, the factorial analysis pointed a unifactorial structure called positive parent-child interaction

Script concordance test: uma proposta de avaliação do raciocínio gerencial da enfermagem em situações de incertezas

Pós-graduação em Enfermagem - FMB

Estudos biológicos de nanopartículas de óxido de zinco: interação com proteínas e avaliação de “Burst” oxidativo de leucócitos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Construção e validação de um túnel de vento para ensaios de estimativa da deriva em pulverizações agrícolas

The aim of this study was to build and validate a low cost reduced-scale wind tunnel for drift evaluation on pesticide application technology. The work was carried out at the NEMPA - Núcleo de Ensaio de Máquinas e Pneus Agroflorestais (NEMPA), FCA/UNESP, Departamento de Engenharia Rural, Botucatu/SP, Brazil. The wind tunnel main characteristics were an open circuit and a closed working section with a fan blowing air into the tunnel. Screens were fitted downstream after the fan in order to stabilize the air flow on the working section. The tunnel was built with 3.0 mm eucalyptus hardboard, with a total length of 4.8 m and a squared section of 0.56 m. The air flow was provided by a 180 W axial fan. The system was adjusted and calibrated to provide a laminar and stable flow at 2.0 m s-1. Validation studies were carried out by using a Teejet XR 8003 flat fan nozzle at 200 kPa (medium droplets) to apply a spray solutions containing water plus a food dye (Blue FDC) at 0,6% m v-1 mixed with two adjuvants: a polymer based anti drift formulation at 0,06% m v-1 and a sodium lauryl ether sulfate based surfactant at 0,2% v v-1. After a 10-second application the drift was collected on nylon strips transversally fixed along the tunnel at different distances from the nozzle and different high from the bottom part of the tunnel. Drift deposits were evaluated by spectrophotometry. The wind tunnel had low levels of turbulence and high repeatability of the data, which means that the flow was uniform and able to be used for carrying out measures to estimate drift. The validation results showed that the tunnel was effective to enable comparative drift measurements on the spray solution used in this work making possible the evaluation of drift risk potential under those spray technologies. The use of an adjuvant based on a polymer reduced the amount of drift from the nozzle compared to the surfactant.

Avaliação de incubadoras neonatais e práticas de manuseio pela equipe de enfermagem

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Relações entre o construto e as características do teste escrito do EPPLE eletrônico: um processo de validação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento e validação de método bioanalítico aplicado em estudo de farmacocinética pré-clínica e ensaio preliminar de segurança de novo derivado de tiazolidinodiona com atividade antiinflamatória - LYSO-07

Pós-graduação em Ciências Farmacêuticas - FCFAR

Expressão gênica e protéica de receptores Toll-like em células do sangue periférico materno de gestações normais e complicadas por prematuridade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Paciente laringectomizado total: validação das características definidoras para o diagnóstico de enfermagem comunicação prejudicada

Este estudo teve por objetivo validar as características definidoras do diagnóstico de enfermagem "Comunicação prejudicada ao paciente submetido à laringectomia total." Para sua realização, optou-se pelo modelo validação de conteúdo diagnóstico (CDV) de FEHRING (1986, 1987). Coletaram-se os dados por meio de uma escala de freqüência do tipo Likert, composta por vinte e seis características definidoras da lista oficial da North American Nursing Diagnosis Association (NANDA) e três características definidoras fictícias. Participaram do estudo vinte e seis enfermeiros assistenciais e docentes. Os resultados demonstraram uma CDV total de 0,84 para as características definidoras maiores e CDV total de 0,69 para as características definidoras menores. Reconheceu-se que o estudo validou as características definidoras preconizadas pela NANDA para o diagnóstico de enfermagem comunicação prejudicada.

Desenvolvimento e validação de método bioanalítico aplicado em estudo de farmacocinética pré-clínica e ensaio preliminar de segurança de novo derivado de tiazolidinodiona com atividade antiinflamatória - LYSO-07

Pós-graduação em Ciências Farmacêuticas - FCFAR

Expressão gênica e protéica de receptores Toll-like em células do sangue periférico materno de gestações normais e complicadas por prematuridade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Eficácia da auriculoterapia para diminuição de estresse em estudantes de enfermagem: ensaio clínico randomizado

Trata-se de estudo clínico randomizado, simples cego, com o objetivo de avaliar a eficácia da auriculoterapia verdadeira e placebo na diminuição dos níveis de estresse em alunos de nível médio, da Escola de Enfermagem do Hospital Beneficência Portuguesa. Setenta e um estudantes com escores médio, alto e altíssimo, pela Lista de Sintomas de Estresse de Vasconcellos, foram divididos em 3 grupos: controle (25), auriculoterapia (24) e placebo/Sham (22). Foram avaliados, no início, com 8, 12 sessões e follow-up (15 dias) e receberam os pontos Shen Men, tronco cerebral (auriculoterapia) e punho e ouvido externo (placebo/Sham). Na análise de variância (Anova) constataram-se diferenças estatísticas significativas entre os grupos controle/auriculoterapia a partir de 8 sessões, mantendo-se após a terceira e a quarta avaliação (p=0,000) e entre controle/placebo (p<0,05), nas três avaliações. Concluiu-se que a auriculoterapia verdadeira obteve melhores respostas (45,39%) do que o placebo (34,18%) na redução do estresse, mas recomendam-se mais estudos para reavaliação de pontos Sham para estresse. ClinicalTrials.gov Identifier: NCT01420848.