Proceedings of the 1st Seminar on Higher Education Rankings and E-learning

First International Seminar on Higher EducationRankings and e-Learning. Proceedings

Detecção de Begomovirus em maracujazeiro (Passiflora edulis Sims) no Estado de Alagoas

O maracujazeiro é uma cultura de grande importância econômica nos países tropicais, destacando-se o Brasil como o maior produtor. No presente trabalho, é relatada a infecção de plantas de maracujazeiro por um vírus do gênero Begomovirus, família Geminiviridae, no município de Anadia, Estado de Alagoas. As plantas infectadas apresentavam sintomas de mosaico amarelo, redução drástica de crescimento e da área foliar. DNA extraído de plantas sintomáticas foi empregado como molde em PCRs, contendo os pares de primers PAL1v1978 e par1C496 ou PBL1v2040 e PCRc1 que direcionam, respectivamente, a amplificação de regiões de aproximadamente 1,2 kb do DNA-A e 0,6 kb do DNA-B, do genoma dos begomovírus. Fragmentos de tamanho esperado foram amplificados a partir de amostras de plantas sintomáticas, confirmando a infecção por Begomovirus. O seqüenciamento desses fragmentos está em andamento para uma definição precisa da espécie viral.

Traumatic symptoms in sexually abused children : implications for school counselors

School counselors have a duty to formulate strategies that aid in the detection and prevention of child sexual abuse (American School Counselor Association, 2003). School counselors are charged with helping sexually abused children by recognizing sexual abuse indicators based on a child's symptomology and/or behavior, and understanding how this trauma may affect children in the school setting. Mandated reporting issues, talking with children and adolescents about sexual abuse suspicions, and understanding trauma symptoms and their contribution to the difficulties that sexually abused children have in school are highlighted. In addition, how school counselors can collaborate with clinicians treating sexually abused children through role-appropriate advocacy, intervention, and aftercare strategies is described.

Conservação de produto minimamente processado de goiabas 'Kumagai' e 'Pedro Sato'

O processamento mínimo permite a obtenção de produtos com qualidade, frescor e conveniência para o consumidor. O objetivo deste trabalho foi determinar os efeitos de tipos de corte e de temperaturas de armazenamento na conservação de produto minimamente processado de goiabas 'Kumagai' e 'Pedro Sato', associando-se goiabas de polpa branca e de polpa vermelha num único produto, como forma de torná-lo mais atraente e nutritivo. Os frutos foram minimamente processados em rodelas e fatias, acondicionados em bandejas de poliestireno expandido, recobertas com filme de PVC 17µm e armazenadas a 5ºC e 15ºC, durante 9 dias. As análises foram realizadas a cada 3 dias quanto às suas características físicas e químicas (firmeza do mesocarpo e da região placentária, cor da casca e da região placentária e teores de sólidos solúveis, acidez titulável e ácido ascórbico) e sensoriais (aparência). As temperaturas e os tipos de cortes não influenciaram nos conteúdos de ácido ascórbico, sólidos solúveis e acidez titulável. O corte tipo rodela manteve por mais tempo a firmeza do mesocarpo e da região placentária. A temperatura de 5ºC preservou a cor da casca, enquanto a de 15ºC e o corte tipo fatia implicaram maior escurecimento da polpa na região placentária. O produto minimamente processado mantido a 5ºC, bem como o corte em rodelas foram os preferidos pelos provadores

The Next Step: Darwin, Brontë, Fowles

The present article proposes Heathcliff and Sarah Woodruff as monstrous beings who reclaim their desire to be agent subjects in a society and a narrative which deny such a possibility. It would be possible to argue, however, that their monstrosity might be that of the unique specimen, the potential first stage towards the improvement of species through natural selection as theorized by Charles Darwin in 1859. The multiple references to Darwin’s study in the novel by JohnFowles demonstrate that such a theory could clarify what Sarah represents in the novel. In a retroactive manner, Darwinian theory might be used to understand what Heathcliff is, who Heathcliff is, and why he is the object of general animosity. It might be concluded that what is really monstrous about these twocharacters is that both are new specimens, avant la lèttre, and they occupy a space to which language has no access.

Comunidad literaria y deconstrucción. Entre el fin del relato y la archi-escritura: Blanchot, Kofman, Derrida

El presente ensayo se propone relacionar tres figuras del texto literario después de la deconstrucción de la comunidad: el fin del relato literario en La folie du jour y"L"Idylle" de Maurice Blanchot, la palabra sofocada [La parole soffoquées] de Sarah Kofman y la archi-escritura en el pensamiento de Jacques Derrida. Abordaremos la escritura como un gesto filosófico con la finalidad de pensar la interrelación de lo literario y lo filosófico en estos autores franceses de la segunda mitad del siglo xx.

Comunidad literaria y deconstrucción. Entre el fin del relato y la archi-escritura: Blanchot, Kofman, Derrida

El presente ensayo se propone relacionar tres figuras del texto literario después de la deconstrucción de la comunidad: el fin del relato literario en La folie du jour y"L"Idylle" de Maurice Blanchot, la palabra sofocada [La parole soffoquées] de Sarah Kofman y la archi-escritura en el pensamiento de Jacques Derrida. Abordaremos la escritura como un gesto filosófico con la finalidad de pensar la interrelación de lo literario y lo filosófico en estos autores franceses de la segunda mitad del siglo xx.

La Calypso: nos offres en soins

Après ses premiers 8 mois de fonctionnement, présentation de La Calypso, unité de soins spécifique de prise en charge pour des sevrages Haut Seuil

Clinical determinants of early parasitological response to ACTs in African patients with uncomplicated falciparum malaria: a literature review and meta-analysis of individual patient data.

BACKGROUND: Artemisinin-resistant Plasmodium falciparum has emerged in the Greater Mekong sub-region and poses a major global public health threat. Slow parasite clearance is a key clinical manifestation of reduced susceptibility to artemisinin. This study was designed to establish the baseline values for clearance in patients from Sub-Saharan African countries with uncomplicated malaria treated with artemisinin-based combination therapies (ACTs). METHODS: A literature review in PubMed was conducted in March 2013 to identify all prospective clinical trials (uncontrolled trials, controlled trials and randomized controlled trials), including ACTs conducted in Sub-Saharan Africa, between 1960 and 2012. Individual patient data from these studies were shared with the WorldWide Antimalarial Resistance Network (WWARN) and pooled using an a priori statistical analytical plan. Factors affecting early parasitological response were investigated using logistic regression with study sites fitted as a random effect. The risk of bias in included studies was evaluated based on study design, methodology and missing data. RESULTS: In total, 29,493 patients from 84 clinical trials were included in the analysis, treated with artemether-lumefantrine (n = 13,664), artesunate-amodiaquine (n = 11,337) and dihydroartemisinin-piperaquine (n = 4,492). The overall parasite clearance rate was rapid. The parasite positivity rate (PPR) decreased from 59.7 % (95 % CI: 54.5-64.9) on day 1 to 6.7 % (95 % CI: 4.8-8.7) on day 2 and 0.9 % (95 % CI: 0.5-1.2) on day 3. The 95th percentile of observed day 3 PPR was 5.3 %. Independent risk factors predictive of day 3 positivity were: high baseline parasitaemia (adjusted odds ratio (AOR) = 1.16 (95 % CI: 1.08-1.25); per 2-fold increase in parasite density, P <0.001); fever (>37.5 °C) (AOR = 1.50 (95 % CI: 1.06-2.13), P = 0.022); severe anaemia (AOR = 2.04 (95 % CI: 1.21-3.44), P = 0.008); areas of low/moderate transmission setting (AOR = 2.71 (95 % CI: 1.38-5.36), P = 0.004); and treatment with the loose formulation of artesunate-amodiaquine (AOR = 2.27 (95 % CI: 1.14-4.51), P = 0.020, compared to dihydroartemisinin-piperaquine). CONCLUSIONS: The three ACTs assessed in this analysis continue to achieve rapid early parasitological clearance across the sites assessed in Sub-Saharan Africa. A threshold of 5 % day 3 parasite positivity from a minimum sample size of 50 patients provides a more sensitive benchmark in Sub-Saharan Africa compared to the current recommended threshold of 10 % to trigger further investigation of artemisinin susceptibility.

An Advocacy for the Use of 3D Stem Cell Culture Systems for the Development of Regenerative Medicine: An Emphasis on Photoreceptor Generation

The availability of stem cells is of great promise to study early developmental stages and to generate adequate cells for cell transfer therapies. Although many researchers using stem cells were successful in dissecting intrinsic and extrinsic mechanisms and in generating specific cell phenotypes, few of the stem cells or the differentiated cells show the capacity to repair a tissue. Advances in cell and stem cell cultivation during the last years made tremendous progress in the generation of bona fide differentiated cells able to integrate into a tissue after transplantation, opening new perspectives for developmental biology studies and for regenerative medicine. In this review, we focus on the main works attempting to create in vitro conditions mimicking the natural environment of CNS structures such as the neural tube and its development in different brain region areas including the optic cup. The use of protocols growing cells in 3D organoids is a key strategy to produce cells resembling endogenous ones. An emphasis on the generation of retina tissue and photoreceptor cells is provided to highlight the promising developments in this field. Other examples are presented and discussed, such as the formation of cortical tissue, the epithelial gut or the kidney organoids. The generation of differentiated tissues and well-defined cell phenotypes from embryonic stem (ES) cells or induced pluripotent cells (iPSCs) opens several new strategies in the field of biology and regenerative medicine. A 3D organ/tissue development in vitro derived from human cells brings a unique tool to study human cell biology and pathophysiology of an organ or a specific cell population. The perspective of tissue repair is discussed as well as the necessity of cell banking to accelerate the progress of this promising field.

Graduating 4th year radiology residents' perception of optimal imaging modalities for neoplasm and trauma: a pilot study from four U.S. universities

OBJECTIVE: Our purpose was to assess 4th year radiology residents' perception of the optimal imaging modality to investigate neoplasm and trauma. MATERIALS AND METHODS: Twenty-seven 4th year radiology residents from four residency programs were surveyed. They were asked about the best imaging modality to evaluate the brain and spine, lungs, abdomen, and the musculoskeletal system. Imaging modalities available were MRI, CT, ultrasound, PET, and X-ray. All findings were compared to the ACR appropriateness criteria. RESULTS: MRI was chosen as the best imaging modality to evaluate brain, spine, abdominal, and musculoskeletal neoplasm in 96.3%, 100%, 70.4%, and 63% of residents, respectively. CT was chosen by 88.9% to evaluate neoplasm of the lung. Optimal imaging modality to evaluate trauma was CT for brain injuries (100%), spine (92.6%), lung (96.3%), abdomen (92.6%), and major musculoskeletal trauma (74.1%); MRI was chosen for sports injury (96.3%). There was agreement with ACR appropriateness criteria. CONCLUSION: Residents' perception of the best imaging modalities for neoplasm and trauma concurred with the appropriateness criteria by the ACR.