Production of the Quinone-Methide Triterpene Maytenin by In Vitro Adventitious Roots of Peritassa campestris (Cambess.) ACSm. (Celastraceae) and Rapid Detection and Identification by APCI-IT-MS/MS

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Um estudo da recente transformação na estrutura social do Brasil: nova classe média?

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Metabolic profiling by ultra-performance liquid chromatography-mass spectrometry and parallel factor analysis for the determination of disease biomarkers in Eucalyptus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A cultura corporal na mídia: possibilidades pedagógicas para a educação física escolar

The Physical education has as objective the students integration in the body movement culture, forming citizens to reframe different cultural aspects of the physical activity, enabling a more critical look. Currently selecting how the body movement culture has been widely approached by Mass Media it is detected a new pedagogical possibility. The several media equipment massively bomb (images, sounds and information) interfering on how the students interpret the reality, since they are exposed to these multiple information and at the same time are absent of a more critical look about what they receive. In this way, the objective of this work was to perform a research in the news/articles from the Jornal A Folha de São Paulo, that allows the development of classes based on the elements of body movement culture and apply them in a public school from Rio Claro-SP city, to detect possibilities and difficulties in the work with the media in the school environment. It employed a qualitative research. The data obtained in this study indicate that the media shows up as a good teaching tool for teaching physical education classes, since students found the classes to enriching the learning process, highlighting topics such as innovation and the closeness they had with the worked information. However, it is necessary to develop further studies to raise other possibilities as well as alternatives to deal with this new reality of the school context

Inorganic elemental analysis and identification of residual monomers released from different glass ionomer cements in cell culture medium

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Mídia esportiva e a profissão de treinador de futebol: estudo de caso sobre o Mundial de Clubes da FIFA

Pós-graduação em Ciências da Motricidade - IBRC

Eleições, mídia e memória: uma análise do jornal Folha de S. Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identification techniques for highly boosted W bosons that decay into hadrons

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Buffalo cheese whey proteins, identification of a 24 kda protein and characterization of their hydrolysates: in vitro gastrointestinal digestion

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Políticas públicas e direitos de crianças e adolescentes: o papel da mídia na expansão da cidadania

This paper presents results of an evaluation of the quality of the coverage of issues associated to the preservation of rights of children and adolescents in the eight main newspapers of major cities of Bauru (Sao Paulo State) administrative region, from August 2008 to March 2009. In a total of 389 assessed stories, 66% portrayed acts of legal infraction and violence with children and adolescents as victims or perpetrators, with a fragmented and superficial perspective; 34% are greater approaches about public policies aimed at the preservation of social rights. The results show the existence of a smaller number, yet significant, of stories which presented extended and more contextualized approaches. From a general view, we can point out that the contribution given by the media as a form of vigilance over the execution of public policies has been limited, which justifies the concern of demanding, from the mass media, measures to fulfill the role attributed to them by contemporaneous democracies to the strengthening of citizenship.

Mídia-educação: da criatividade à livre expressão na escola

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of comprehensive two-dimensional gas chromatography coupled to rapid scanning quadrupole mass spectrometry for quantitative analysis

Comprehensive two-dimensional gas chromatography (GC x GC) is a powerful technique that provides excellent separation and identification of analytes in highly complex samples with considerable increase in GC peak capacities. However, since second dimension analyses are very fast, detectors with a rapid acquisition rate are required. Over the last years, quite a number of studies have discussed the potential and limitations of the combination GC x GC with a variety of quadrupole mass spectrometers. The present research focuses on the evaluation of qMS effectiveness at a 10,000-amu/s scan speed and 20-Hz scan frequency for the identification (full scan mode acquisition-TIC) and quantification (extracted ion chromatogram) of target pesticide residues in tomato samples. The following MS parameters have been evaluated: number of data points per peak, mass spectrum quality, peak skewing, and sensitivity. The validated proposed GC x GC/qMS method presented satisfactory results in terms of repeatability (coefficient of variation lower than 15%), accuracy (84-117%), and linearity (ranging from 25 to 500 ng/g), while significant enhancement in sensitivity was observed (a factor of around 10) under scan conditions. (C) 2012 Elsevier B.V. All rights reserved.

Identification of Leishmania infantum chagasi proteins in urine of patients with visceral leishmaniasis: a promising antigen discovery approach of vaccine candidates

Visceral leishmaniasis (VL) is a serious lethal parasitic disease caused by Leishmania donovani in Asia and by Leishmania infantum chagasi in southern Europe and South America. VL is endemic in 47 countries with an annual incidence estimated to be 500 000 cases. This high incidence is due in part to the lack of an efficacious vaccine. Here, we introduce an innovative approach to directly identify parasite vaccine candidate antigens that are abundantly produced in vivo in humans with VL. We combined RP-HPLC and mass spectrometry and categorized three L. infantum chagasi proteins, presumably produced in spleen, liver and bone marrow lesions and excreted in the patients urine. Specifically, these proteins were the following: Li-isd1 (XP_001467866.1), Li-txn1 (XP_001466642.1) and Li-ntf2 (XP_001463738.1). Initial vaccine validation studies were performed with the rLi-ntf2 protein produced in Escherichia coli mixed with the adjuvant BpMPLA-SE. This formulation stimulated potent Th1 response in BALB/c mice. Compared to control animals, mice immunized with Li-ntf2+ BpMPLA-SE had a marked parasite burden reduction in spleens at 40 days post-challenge with virulent L. infantum chagasi. These results strongly support the proposed antigen discovery strategy of vaccine candidates to VL and opens novel possibilities for vaccine development to other serious infectious diseases.

Multi-element determination in Brazilian honey samples by inductively coupled plasma mass spectrometry and estimation of geographic origin with data mining techniques

Multi-element analysis of honey samples was carried out with the aim of developing a reliable method of tracing the origin of honey. Forty-two chemical elements were determined (Al, Cu, Pb, Zn, Mn, Cd, Tl, Co, Ni, Rb, Ba, Be, Bi, U, V, Fe, Pt, Pd, Te, Hf, Mo, Sn, Sb, P, La, Mg, I, Sm, Tb, Dy, Sd, Th, Pr, Nd, Tm, Yb, Lu, Gd, Ho, Er, Ce, Cr) by inductively coupled plasma mass spectrometry (ICP-MS). Then, three machine learning tools for classification and two for attribute selection were applied in order to prove that it is possible to use data mining tools to find the region where honey originated. Our results clearly demonstrate the potential of Support Vector Machine (SVM), Multilayer Perceptron (MLP) and Random Forest (RF) chemometric tools for honey origin identification. Moreover, the selection tools allowed a reduction from 42 trace element concentrations to only 5. (C) 2012 Elsevier Ltd. All rights reserved.

Identification of intracellular peptides in rat adipose tissue: Insights into insulin resistance

Intracellular peptides generated by the proteasome and oligopeptidases have been suggested to function in signal transduction and to improve insulin resistance in mice fed a high-caloric diet. The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats that could be associated with the physiological and therapeutic control of glucose uptake. Using semiquantitative mass spectrometry and LC/MS/MS analyses, we identified ten peptides in the epididymal adipose tissue of the Wistar rats; three of these peptides were present at increased levels in rats that were fed a high-caloric Western diet (WD) compared with rats fed a control diet (CD). The results of affinity chromatography suggested that in the cytoplasm of epididymal adipose tissue from either WD or CD rats, distinctive proteins bind to these peptides. However, despite the observed increase in the WD animals, the evaluated peptides increased insulin-stimulated glucose uptake in 3T3-L1 adipocytes treated with palmitate. Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin-induced glucose uptake in 3T3-L1 adipocytes.