Effects of endogenous flour lipids on the quality of semisweet biscuits

Fractionation and reconstitution techniques were used to study the contribution of enclogenous flour lipids to the quality of semisweet (Rich Tea-type) biscuits. Biscuit flour was defatted with chloroform and baked with bakery fat but without enclogenous lipid addition. Semisweet biscuits baked from defatted flour were flatter, denser, and harder and showed collapse of gas cells during baking when compared with control biscuits. Defatted flour semisweet doughs exhibited a different rheological behavior from the control samples showing higher storage and loss moduli (G' and G" values), that is, high viscoelasticity. Functionality was restored when total nonstarch flour lipids were added back to defatted flour. Both the polar and nonpolar lipid fractions had positive effects in restoring flour quality, but the polar lipid fraction was of greatest benefit. Both fractions were needed for complete restoration of both biscuit quality and dough rheological characteristics.

Effects of endogenous flour lipids on the quality of short-dough biscuits

Fractionation and reconstitution techniques were used to study the contribution of endogenous flour lipids to the quality of short-dough (shortcake type) biscuits. Biscuit flour was defatted with chloroform and baked with bakery fat, but without endogenous lipid. Short-dough biscuits baked from defatted flour had smaller diameters, and were flatter, denser and harder than control biscuits. Defatted flour shortcake doughs exhibited different rheological behaviour from the control samples, showing higher storage and loss moduli (G' and G" values), ie higher viscoelasticity. Functionality was restored when total non-starch flour lipids were added back to defatted flour. The polar lipid fraction had a positive effect in restoring flour quality whereas the non-polar lipid fraction had no effect. Both fractions were needed for complete restoration of both biscuit quality and dough rheological characteristics. A study of the microstructure of defatted biscuits revealed that their gluten protein was more hydrated and developed than the gluten of the control biscuits. This conclusion was supported by the higher water absorption of the defatted gluten. (C) 2004 Society of Chemical Industry.

Determination of digesta flow entering the omasal canal of dairy cows using different marker systems

Four studies were conducted to compare the effect of four indigestible markers (LiCoEDTA, Yb-acetate, Cr-mordanted straw and indigestible neutral-detergent fibre (INDF)) and three marker systems on the flow of digesta entering the omasal canal of lactating dairy cows. Samples of digesta aspirated from the omasal canal were pooled and separated using filtration and high-speed centrifugation into three fractions defined as the liquid phase, small particulate and large particulate matter. Co was primarily associated with the liquid phase, Yb was concentrated in small particulate matter, whilst Cr and INDF were associated with large particles. Digesta flow was calculated based on single markers or using the reconstitution system based on combinations of two (Co + Yb, Co + Cr and Co + INDF) or three markers (Co + Yb + Cr and Co + Yb + INDF). Use of single markers resulted in large differences between estimates of organic matter (OM) flow entering the omasal canal suggesting that samples were not representative of true digesta. Digesta appeared to consist of at least three phases that tended to separate during sampling. OM was concentrated in particulate matter, whilst the liquid phase consisted mainly of volatile fatty acids and inorganic matter. Yb was intimately associated with nitrogenous compounds, whereas Cr and INDF were concentrated in fibrous material. Current data indicated that marker systems based on Yb in combination with Cr or INDF are required for the accurate determination of OM, N and neutral-detergent fibre flow. In cases where the flow of water-soluble nutrients entering the omasal canal is also required, the marker system should also include Co.

Survival of freeze dried Lactobacillus plantarum in instant fruit powders and reconstituted fruit juices

The aim of this study was to investigate the survival of freeze dried Lactobacillus plantarum cells mixed with several freeze dried instant fruit powders (strawberry, pomegranate, blackcurrant and cranberry) during storage for 12 months as well as after reconstitution with water each month. Inulin and gum arabic were also added to the instant fruit powders at two levels (10% and 20% w/w of dry weight) to improve the cell survival and functional properties of the product. The best cell survival over the 12 months of storage was observed for the blackcurrant powder (almost no decrease) followed by strawberry (~ 0.3 log decrease), pomegranate (~ 0.9 log decrease), whereas the worst survival was obtained in cranberry powder (~ 4.5 logs). To explain these results multiple regression analysis was conducted with the log decrease [log10N0 month − log10N12 months] as the dependent variable and water activity, pH, citric acid, dietary fibre and total phenol as the independent variables. The results indicated that among all the examined factors, the [log10N0 month − log10N12 months] depended only on the water activity (P < 0.05). Inulin and gum arabic demonstrated a substantial protective effect on cell survival (1–1.5 log) in the case of cranberry, which was likely due to a physical interaction between the cells and the carbohydrates. After reconstituting the dried fruit powders at room temperature and measuring cell viability for up to 4 h, it was shown that in the case of strawberry juice there was no decrease, and very little in the case of pomegranate and blackcurrant juices (< 0.5 log). On the other hand, a significant decrease was observed for cranberry juice (P < 0.05), which increased as the storage time of the dried cranberry powder increased, indicating that the cells became more susceptible with prolonged storage. Multiple regression analysis indicated that the main factors influencing cell survival were water activity and pH, while citric acid, dietary fibre and total phenol did not have an effect. Furthermore, inulin and gum arabic addition did not have a significant (P > 0.05) effect upon reconstitution of the dried fruit powder. This study showed that instant juice powders are very good carriers of probiotic cells and constitute good alternatives to highly acidic fruit juices.

Is there a gold social seal? The financial effects of additions to and deletions from social stock indices

This study investigates the financial effects of additions to and deletions from the most well-known social stock index: the MSCI KLD 400. Our study makes use of the unique setting that index reconstitution provides and allows us to bypass possible issues of endogeneity that commonly plague empirical studies of the link between corporate social and financial performance. By examining not only short-term returns but also trading activity, earnings per share, and long-term performance of stocks that are involved in these events, we bring forward evidence of a ‘social index effect’ where unethical transgressions are penalized more heavily than responsibility is rewarded. We find that the addition of a stock to the index does not lead to material changes in its market price, whereas deletions are accompanied by negative cumulative abnormal returns. Trading volumes for deleted stocks are significantly increased on the event date, while the operational performances of the respective firms deteriorate after their deletion from the social index.

Role of alpha 7 Nicotinic Acetylcholine Receptor in Calcium Signaling Induced by Prion Protein Interaction with Stress-inducible Protein 1

The prion protein (PrP(C)) is a conserved glycosylphosphatidyl-inositol-anchored cell surface protein expressed by neurons and other cells. Stress-inducible protein 1 (STI1) binds PrP(C) extracellularly, and this activated signaling complex promotes neuronal differentiation and neuroprotection via the extracellular signal-regulated kinase 1 and 2 (ERK1/2) and cAMP-dependent protein kinase 1 (PKA) pathways. However, the mechanism by which the PrPC-STI1 interaction transduces extracellular signals to the intracellular environment is unknown. We found that in hippocampal neurons, STI1-PrP(C) engagement induces an increase in intracellular Ca(2+) levels. This effect was not detected in PrP(C)-null neurons or wild-type neurons treated with an STI1 mutant unable to bind PrP(C). Using a best candidate approach to test for potential channels involved in Ca(2+) influx evoked by STI1-PrP(C), we found that alpha-bungarotoxin, a specific inhibitor for alpha 7 nicotinic acetylcholine receptor (alpha 7nAChR), was able to block PrP(C)-STI1-mediated signaling, neuroprotection, and neuritogenesis. Importantly, when alpha 7nAChR was transfected into HEK 293 cells, it formed a functional complex with PrP(C) and allowed reconstitution of signaling by PrP(C)-STI1 interaction. These results indicate that STI1 can interact with the PrP(C).alpha 7nAChR complex to promote signaling and provide a novel potential target for modulation of the effects of prion protein in neurodegenerative diseases.

Germline CYBB mutations that selectively affect macrophages in kindreds with X-linked predisposition to tuberculous mycobacterial disease

Germline mutations in CYBB, the human gene encoding the gp91(phox) subunit of the phagocyte NADPH oxidase, impair the respiratory burst of all types of phagocytes and result in X-linked chronic granulomatous disease (CGD). We report here two kindreds in which otherwise healthy male adults developed X-linked recessive Mendelian susceptibility to mycobacterial disease (MSMD) syndromes. These patients had previously unknown mutations in CYBB that resulted in an impaired respiratory burst in monocyte-derived macrophages but not in monocytes or granulocytes. The macrophage-specific functional consequences of the germline mutation resulted from cell-specific impairment in the assembly of the NADPH oxidase. This `experiment of nature` indicates that CYBB is associated with MSMD and demonstrates that the respiratory burst in human macrophages is a crucial mechanism for protective immunity to tuberculous mycobacteria.

Historical carbon budget of the brazilian ethanol program

This work models the carbon neutralization capacity of Brazil`s ethanol program since 1975. In addition to biofuel, we also assessed the mitigation potential of other energy products, such as, bioelectricity, and CO(2) emissions captured during fermentation of sugar cane`s juice. Finally, we projected the neutralization capacity of sugar cane`s bio-energy system over the next 32 years. The balance between several carbon stocks and flows was considered in the model, including the effects of land-use change. Our results show that the neutralization of the carbon released due to land-use change was attained only in 1992, and the maximum mitigation potential of the sugar cane sector was 128 tonnes Of CO(2) per ha in 2006. An ideal reconstitution of the deployment of the sugar cane sector, including the full exploitation of bio-electricity`s potential, plus the capture Of CO(2) released during fermentation, shows that the neutralization of land-use change emissions would have been achieved in 1988, and its mitigation potential would have been 390 tCO(2)/ha. Finally, forecasts of the sector up to 2039 shows that the mitigation potential in 2039 corresponds to 836 tCO(2)/ha, which corresponds to 5.51 kg Of CO(2) per liter of ethanol produced, or 55% above the negative emission level. (C) 2009 Elsevier Ltd. All rights reserved.

Correlations between chemical composition and provenance of Justino site ceramics by INAA

Instrumental neutron activation analysis (INAA), have been used for the definition of compositional groups of potteries from Justino site, Brazil, according to the chemical similarities of ceramic paste. The outliers were identified by means of robust Mahalanobis distance. The temper effect in the ceramic paste was studied by means of modified Mahalanobis filter. The results were interpreted by means of cluster, principal components, and discriminant analyses. This work provides contributions for the reconstruction of the prehistory of baixo Sao Francisco region, and for the reconstitution of the Brazilian Northeast ceramist population of general frame.

Origem e evolução do partido comunista brasileiro revolucionário : (1967-1973)

Este trabalho busca resgatar a origem e história de uma das organizações de esquerda que surgiu no período de 1968 a partir das dissidências dentro do Partido Comunista Brasileiro. Inúmeras organizações irão surgir nesse período, sendo que a maioria das mesmas irá aderir ao processo de luta armada contra o regime militar. Entre as diversas diferenças políticas e ideológicas entre elas, uma se destacava: a manutenção de uma estrutura de partido ou a criação de organizações sem estrutura de partido e com liberdade de ação por parte dos comandos militares da organização. O fundo dessa discordância era proveniente da influência da Revolução Cubana entre os militantes de esquerda na América Latina através da Teoria do Foco, onde a ação de partido era considerada desnecessária. Por outro lado, também é fruto da retomada dos debates sobre concepção de partido revolucionário e a relação dos partidos com os movimentos de massas, debate presente no pensamento marxista europeu durante toda a primeira metade do século XX. O PCBR buscou aliar a concepção leninista de partido com as posições de defesa da autonomia do movimento de massas frente aos partidos, concepção defendida pela marxista alemã Rosa Luxemburgo. O trabalho, portanto, busca estudar o contexto em que este debate se realizou na esquerda brasileira durante o período militar. Por fim, o presente estudo buscou também contribuir com a reconstituição histórica de alguns fatos relevante relacionados à ação política do PCBR durante o período militar.

Feira de São Cristovão: a reconstrução do nordestino num mundo de paraíbas e nortistas

A presente dissertação estuda uma feira nordestina, a Feira de são Cristóvao, situada na zona norte do Rio de Janeiro e que se realiza há mais de trinta anos. Mostra o surgimento desta Feira como vinculado ao fenômeno das migrações nordestinas para as grandes cidades do Centro-Sul nos primeiros anos da década de 50. Reconstitui a história da Feira onde se evidencia a existência de formas associativas. Estas, disputaram entre si, através de mecanismos autoritários, o domínio e a exploração da Feira até a sua legalização em 1982. Focaliza a maneira como o nordestino migrante se apropriou do espaço da Feira, registrando as principais características desse espaço. Discute o significado de paraiba e nortista. Paraiba, denominação de cunho depreciativo, é dada ao migrante originário do Nordeste pelos demais habitantes da cidade; nortista, é a autodenominação que o nordestino se atribui para sua identificação. Analisa, como uma das manifestações específicas da Feira, a produção e venda da comida regional. Esta é produzida através de práticas e técnicas sedimentadas na educação do senso comum caracterizando um tipo de saber que é próprio das classes populares. Finalmente, trata de conhecer e interpretar a Feira de São Cristóvão como um espaço de expressão da cultura popular que contribui no processo de reconstrução da identidade do nordestino na cidade que o batizou de paraíba.

O discurso educacional do Maranhão na Primeira República: uma análise de conteúdo

Esta pesquisa, a partir do pressuposto de que na sociedade maranhense da Primeira República já existia uma divisão social de trabalho definida, em que as classes dominantes detinham o conhecimento, criando uma distribuição de saber que legitimava a distribuição na esfera do poder, empreende a reconstituição histórica da sociedade da época bem como do seu sistema educacional, no sentido de contextualizar a análise do discurso de alguns intelectuais maranhenses, procurando mostrar o seu caráter eminentemente conservador e o seu comprometimento com o poder de Estado, na medida em que veiculavam a ideologia oficial e provocavam a alienação das massas. Este estudo tenta mostrar ainda como a educação foi se tornando, com o advento da República, cada vez mais importante em sua função adicional de reprodutora das estruturas de poder e como a escola já objetivava, naquela época, inculcar a ideologia das classes dominantes e reproduzir as diferenças sociais entre os indivíduos.

Monismo do corpo: único ponto de partida atualmente possível para o desenvolvimento de uma psicologia científica

Inicia-se o trabalho, reconstituindo-se, sob um ponto de vista histórico, o problema do dualismo e do monismo, na Psicologia. A reconstituição é feita partindo-se de uma origem situada em Sócrates e, dai, desenvolvendo-se até os dias atuais, onde, demonstra-se, a questão permanece. A identificação daquela origem foi determinada pela circunstância de, alí, o problema ter merecido um estudo sistematizado e ter se caracterizado como metafísico. Entendendo-se, com isso, que a questão a resolver era a respeito do que existiria como entidade autônoma. Neste caso, então, se apenas o "corpo", se apenas a "mente" ou se os dois. As soluções que propunham a existência só da mente (monistas da mente), ou de mente e corpo, enquanto entidades distintas (dualistas) viriam a ser, portanto, decisivas para a própria concepção da Psicologia. Como se afirma ser, a partir de uma decisão referente ao problema anterior, que se deva desenvolver uma Psicologia cientifica, estabelece-se, no capítulo II, as concepções adotadas para Ciência, conhecimento científico e método científico. Ali, aproveita-se para justificar porque parte do estudo deve cair sob o domínio da Filosofia da Ciência, como um todo e da Filosofia da Psicologia, em particular. No capítulo III, volta-se a demonstrar com maior ênfase, que o problema "mente-corpo" é, ainda hoje, metafísico e requer uma tomada de decisão, naqueles termos. Mostra-se que a decisão é sempre tomada quando nada como pressuposto, senão explícito, pelo menos implícito. Uma vez tendo-se demonstrado que só o corpo pode ser afirmado como representando alguma coisa que exista, em termos reais, no sentido metafísico, parte-se para o estabelecimento daquele que seria o autêntico objeto de estudo da Psicologia. Fazendo-se, então, uso de uma Semântica Filosófica "crítico-realista", demonstra-se que ele termina sendo: o conjunto de propriedades do objeto real representado pelo corpo e responsáveis pelas manifestações pelas quais a Psicologia, por uma tradição de investigação, sempre se interessou. Finalmente, no capítulo IV, concebe-se um modelo sistêmico para representar a Natureza. Nele vige a 'lei' da transformação, que resulta da' interação entre os subsistemas. Entre os subsistemas existem aqueles que representam objetos reais e são designados como "Corpo Humano". Estes estão sujeitos à mesma 'lei'. A partir da transformação do U23592 em Pb20782, constrói-se duas funções matemáticas, com base na teoria dos conjuntos, para demonstrar-se como funciona a lei da transformação ou a função transformação, aplicável a todos os subsistemas, que são elementos do Sistema que representa a Natureza. Dessas construções e mais algumas, ao serem aplicadas aos subsistemas que representam os objetos reais denotados como Corpo Humano, extrai-se um grande número de consequências para a Psicologia. Termina-se apresentando um modelo específico para representar o objeto real denotado por Corpo Humano. Este, como subsistema, também é um sistema e composto de quatro subsistemas: Motor, Emocional, Perceptivo e Cognitivo. O todo e as partes passam a funcionar regidos pela lei da Transformação.