Prevalência, Virulência e sensibilidade às terapias antimicrobianas das cepas de enterecoccus faecalis e enterococcus faecium isoladas de infecções endodônticas

Pós-graduação em Biopatologia Bucal - ICT

Biofilmes multiespécies de Candida Albicans associados com streptococcus mitis e streptococcus sanguinis: estudo in vitro e in vivo

Pós-graduação em Odontologia Restauradora - ICT

Dentifrício fluoretado suplementado com hexametafosfato de sódio sobre o processo de desmineralização do esmalte: estudos in vitro e in situ

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação das características de superfície e formação de biofilmes em materiais odontológicos tratados em plasmas de HMDSO

Pós-graduação em Reabilitação Oral - FOAR

Ação da histatina 5, uma proteína antimicrobiana, no desenvolvimento de biofilmes de Candida Albicans

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da água ozonizada e gluconato de clorexidina sobre propriedades mecânicas e microbiológicas de materiais utilizados para confecção de próteses odontológicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Direct restorative treatment of dental erosion caused by gastroesophageal reflux disease associated with bruxism: a case report

Gastroesophageal reflux disease (GERD) is a gastrointestinal disorder in which stomach acids are chronically regurgitated into the esophagus and oral cavity. Continual exposure of the teeth to these acids can cause severe tooth wear. Dentists are often the first healthcare professionals to diagnose dental erosion in patients with GERD. This article presents a case report of a 27-year-old male smoker with tooth wear and dentin sensitivity caused by GERD associated with bruxism. After diagnosis, a multidisciplinary treatment plan was established. The initial treatment approach consisted of medical follow-up with counseling on dietary and smoking habits, as well as management of the gastric disorders with medication. GERD management and the dental treatment performed for the eroded dentition are described, including diagnosis, treatment planning, and restorative therapy.

Influence of corrosion on lipopolysaccharide affinity for two different titanium materials

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relationship between head and neck cancer therapy and some genetic endpoints

Head and neck cancer (HNC) is the sixth most common human malignancy worldwide. The main forms of treatment for HNC are surgery, radiotherapy (RT) and chemotherapy (CT). However, the choice of therapy depends on the tumor staging and approaches, which are aimed at organ preservation. Because of systemic RT and CT genotoxicity, one of the important side effects is a secondary cancer that can result from the activity of radiation and antineoplastic drugs on healthy cells. Ionizing radiation can affect the DNA, causing single and double-strand breaks, DNA-protein crosslinks and oxidative damage. The severity of radiotoxicity can be directly associated with the radiation dosimetry and the dose-volume differences. Regarding CT, cisplatin is still the standard protocol for the treatment of squamous cell carcinoma, the most common cancer located in the oral cavity. However, simultaneous treatment with cisplatin, bleomycin and 5-fluorouracil or treatment with paclitaxel and cisplatin are also used. These drugs can interact with the DNA, causing DNA crosslinks, double and single-strand breaks and changes in gene expression. Currently, the late effects of therapy have become a recurring problem, mainly due to the increased survival of HNC patients. Herein, we present an update of the systemic activity of RT and CT for HNC, with a focus on their toxicogenetic and toxicogenomic effects.

Acrylic resin cytotoxicity for denture base - Literature review

Acrylic resin is a widely used material in clinical practice, and a satisfactory biocompatibility is essential. When the resin polymerization reaction is incomplete, residual monomers are released into the oral cavity. The aim of this study was to evaluate, through a literature review, the cytotoxicity caused by the denture base acrylic resin used, and its components. The selection of published studies was performed on the Pubmed database from January 2008 to July 2013. The keywords used were: cytotoxicity and acrylic resins, cytotoxicity and denture base resins and cytotoxicity and oral prosthesis. Inclusion criteria were: in vitro studies and literature reviews published in English that evaluated the acrylic resin cytotoxicity for denture base and its components. Studies with no reference to the search strategy were excluded. A total of 182 articles were found. Among these, only 13 were included for writing this review. The MTT test is the most common test used to evaluate acrylic resin cytotoxicity. Auto-polymerized resin is more cytotoxic than heat-polymerized resin because of its higher quantity of residual monomers which cause cell and tissue changes in the oral mucosa. However, more studies are necessary for the development of biocompatible materials.

Competitive interactions between C. albicans, C. glabrata and C. krusei during biofilm formation and development of experimental Candidiasis

In this study, we evaluated the interactions between Candida albicans, Candida krusei and Candida glabrata in mixed infections. Initially, these interactions were studied in biofilms formed in vitro. CFU/mL values of C. albicans were lower in mixed biofilms when compared to the single biofilms, verifying 77% and 89% of C. albicans reduction when this species was associated with C. glabrata and C. krusei, respectively. After that, we expanded this study for in vivo host models of experimental candidiasis. G. mellonella larvae were inoculated with monotypic and heterotypic Candida suspensions for analysis of survival rate and quantification of fungal cells in the haemolymph. In the groups with single infections, 100% of the larvae died within 18 h after infection with C. albicans. However, interaction groups achieved 100% mortality after 72 h of infection by C. albicans-C. glabrata and 96 h of infection by C. albicans-C. krusei. C. albicans CFU/mL values from larvae hemolymph were lower in the interacting groups compared with the monoespecies group after 12 h of infection. In addition, immunosuppressed mice were also inoculated with monotypic and heterotypic microbial suspensions to induce oral candidiasis. C. albicans CFU/mL values recovered from oral cavity of mice were higher in the group with single infection by C. albicans than the groups with mixed infections by C. albicans-C. glabrata and C. albicans-C. krusei. Moreover, the group with single infection by C. albicans had a higher degree of hyphae and epithelial changes in the tongue dorsum than the groups with mixed infections. We concluded that single infections by C. albicans were more harmful for animal models than mixed infections with non-albicans species, suggesting that C. albicans establish competitive interactions with C. krusei and C. glabrata during biofilm formation and development of experimental candidiasis.

Spindle cell lipoma occurring in the buccal mucosa: an unusual location of this benign lipomatous neoplasm

Spindle cell lipoma is a benign lipomatous neoplasm, which rarely occurs in the oral cavity. The aims of this paper are to report a case of spindle cell lipoma located in buccal mucosa and discuss the main clinical, histological, and immunohistochemical findings of this entity. Thus, we report a 4-year history of an asymptomatic smooth surface nodule in an elderly Caucasian man with clinical hypothesis of fibroma. The histopathological examination showed spindle cells, mature adipose tissue, and many mast cells in a stroma of connective tissue presenting ropey collagen fibers bundles. After immunohistochemical analysis, the final diagnosis was spindle cell lipoma.

Mensuração da profundidade da porção lateral da fossa mandibular, em imagens radiográficas da ATM, obtidas pela técnica transcraniana, em pacientes com dentição decídua, mista e permanente

The author has verified the average depth of the mandibular fossa, in the X-ray image, using the oblique lateral transcranial technique from the right and left sides samples of each patient, which included a total of 176 patients, 87 male and 89 female. The patients were in following phases: deciduous dentítion (the patients had only deciduous teeth in the oral cavity or, if they had any permanent teeth, they could not be in occlusion), mixed dentition (the patients presented deciduous and permanent in the oral cavity) and permanent dentition (the patients had only permanent teeth in the oral cavity), until the eruption of the permanent third molars, in the region from São José dos Campos. São Paulo. Brazil. The patients were under treatment at the Dental School. UNESP (São Paulo State University). ln order to measure the depth of the mandibular fossa in millimeters an imaginary line was traced on the X-ray image, perpendicular to the other line that served as a reference, which was traced from the botton part of the articular eminence up to the tympanosquamous fissure. After the data were obtained and put in a data sheet, they underwent statistical analysis. The results showed that, in the average, the depth of the mandibular fossa in masculine sex is non-statistically signíficant larger than what was observed in feminíne sex, and the right side is larger than the left side, with significant statistical differences. However, only in permanent dentition, in masculine sex, the depth of the mandibular fossa on the right side is larger than on the left side with significant statistical differences

Fechamento de fístula buco-sinusal usando tecido adiposo bucal

The Oro-antral Fistula is a common pathologic event, which occurs an opening or communication of the maxillary sinus with the oral cavity through dental extractions of upper posterior elements whose roots have close relationship with the maxillary sinus. This study aims to clarify the Surgeon Dentist about the possible etiological factors responsible for Oro-antral Communication, to identify its clinical and radiographic signs, to explain the Buccal Fat Pad’s anatomy and functioning and to describe the surgical technique adopted front of these cases. For this, the authors present a case of a patient who had a fistula in the region where there was a dental extraction of the upper posterior element. The treatment of Oro-antral Fistula using the buccal fat pad provides to be a safe and effective surgical method, because this element presents a rich blood supply and easy access. Many authors have found that this method have a broad application, large index of success, lower risk of infection, provides a comfortable post-operative for the patient. However, it needs to be done properly so that you have minimum incidence of failures, and this requires some caution on the part of professional.

Tumores de cabeça e pescoço: epidemiologia, fatores de risco, diagnóstico e tratamento

Neoplasias of the head and neck correspond to the fifth most common cancer in the world, with high mortality and morbidity. The aim of this study was to show, through a literature review, a little about the epidemiology, risk factors, diagnosis and therapy of the most common malignant tumors of each anatomical region of the head and neck. The research of the articles was made in MEDLINE data base, with a total of 52 articles used. In this review, we analyze the main aspects related to the tumors of oral cavity, pharynx, larynx and salivary glands. Almost all cancers of the head and neck affecting the mucosa of the upper aerodigestive tract are derived from squamous cells. Therefore, the risk factors between the various types of tumors are similar, with alcohol and tobacco being the main ones. To be composed of several distinct structures, the head and neck tumors deserve a diagnostic and therapeutic approach individualized for each anatomical region. Controllingthe risk factors and doing the right approach, it is expected that the incidence of cancers of the head and neck decreases, thereby reducing mortality from malignant neoplasms worldwide