Taenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samples

The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T saginata identification in human fecal samples. (C) 2003 Elsevier B.V. (USA). All rights reserved.

Fecal specimens preparation methods for PCR diagnosis of human taeniosis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Molecular identification of Cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of São Paulo, Brazil

The objective of this study was to obtain information of epidemiological nature through genotypic characterization of Cryptosporidium isolates from dogs, cats and bovines from the state of São Paulo, Brazil. The extraction of DNA from oocysts was carried out and polymerase chain reaction was accomplished using specific primers to 18S rRNA gene. The amplicons were directed sequenced. Seven cat samples, nine dog samples and nine bovine samples were analysed. From the seven cat samples the genotypic analyses revealed Cryptosporidium felis in all. These were the first genotypic characterization of Cryptosporidium from domestic felines in Brazil. In nine sequenced samples from dogs, genotypic identities compatible with Cryptosporidium canis were revealed in all samples. The genotypic analyses in bovines revealed Cryptosporidium parvum in eight samples and Cryptosporidium bovis in another sample, the last one being a non-zoonotic species, not related to clinical symptoms and described for the first time in Brazil. (c) 2007 Elsevier B.V. All rights reserved.

Molecular characterization of Cryptosporidium spp. from fecal samples of birds kept in captivity in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Superstrings in 2D backgrounds with R-R flux and new extremal black holes

The hybrid formalism is used to quantize the superstring compactified to two-dimensional target-space in a manifestly spacetime supersymmetric manner. A quantizable sigma model action is then constructed for the type II superstring in curved two-dimensional supergravity backgrounds which can include Ramond-Ramond flux. Such curved backgrounds include Calabi-Yau fourfold compactifications with Ramond-Ramond flux, and new extremal black hole solutions in two-dimensional dilaton supergravity theory. These black hole solutions are a natural generalization of the CGHS model and might be possible to describe using a supergroup version of the SL(2, R)/U(1) WZW model. We also study some dynamical aspects of the new black holes, such as formation and evaporation. (C) 2001 Published by Elsevier B.V. B.V.

Aspects of classical and quantum motion on a flux cone

Motion of a nonrelativistic particle on a cone with a magnetic flux running through the cone axis (a flux cone) is studied. It is expressed as the motion of a particle moving on the Euclidean plane under the action of a velocity-dependent force. The probability fluid (quantum flow) associated with a particular stationary state is studied close to the singularity, demonstrating nontrivial Aharonov-Bohm effects. For example, it is shown that, near the singularity, quantum flow departs from classical flow. In the context of the hydrodynamical approach to quantum mechanics, quantum potential due to the conical singularity is determined, and the way it affects quantum flow is analyzed. It is shown that the winding number of classical orbits plays a role in the description of the quantum Bow. The connectivity of the configuration space is also discussed.

Conformal field theory of AdS background with Ramond-Ramond flux

We review a formalism of superstring quantization with manifest six-dimensional spacetime supersymmetry, and apply it to AdS(3) x S-3 backgrounds with Ramond-Ramond flux. The resulting description is a conformal field theory based on a sigma model whose target space is a certain supergroup SU' (2\2).

Path integrals on a flux cone

This paper considers the Schrodinger propagator on a cone with the conical singularity carrying magnetic flux (flux cone). Starting from the operator formalism, and then combining techniques of path integration in polar coordinates and in spaces with constraints, the propagator and its path integral representation are derived. The approach shows that effective Lagrangian contains a quantum correction term and that configuration space presents features of nontrivial connectivity.

Taming the b antighost with Ramond-Ramond flux

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Confusing the extragalactic neutrino flux limit with a neutrino propagation limit

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Magnetic flux inversion in charged BPS vortices in a Lorentz-violating Maxwell-Higgs framework

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detection of Cryptosporidium parvum oocysts in calf fecal samples by direct immunofluorescence assay

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos de probióticos sobre a digestibilidade, escore fecal e características hematológicas em cães

Avaliaram-se os efeitos da suplementação de dois tipos de probióticos para cães filhotes, que receberam dois tipos de dieta - de alta e de baixa qualidade -, sobre a digestibilidade dos nutrientes, escore fecal e parâmetros sanguíneos. Foram utilizados 18 animais, distribuídos em três tratamentos. No tratamento 1, controle, os cães receberam somente a ração; no tratamento 2, ração com probiótico 1 (Bifidobacterium) e, no tratamento 3, ração com probiótico 2 (Lactobacillus). O experimento foi dividido em duas fases. Verificaram-se que os valores médios do coeficiente de metabolizabilidade da energia bruta (CMEB) na fase 1, caracterizada pela troca da dieta Super Premium para a dieta Standard, apresentaram resultados significativos (P<0,05), sendo os melhores resultados obtidos nos animais que receberam o probiótico 2. Não houve diferenças significativas (P>0,05) para o escore fecal e para as análises hematológicas.

A Hypercaloric pellet-diet cycle induces obesity and co-morbidities in wistar rats

O objetivo do estudo foi desenvolver um ciclo de dietas hipercalóricas para promover obesidade em ratos. Ratos Wistar foram distribuídos em dois grupos: dieta normal (ND = 32; 3,5 kcal/g) e dietas hipercalóricas (HD; n = 32; 4,6 kcal/g). O grupo ND recebeu ração comercial e os animais HD um ciclo de diferentes dietas hipercalóricas, por 14 semanas. As variáveis analisadas foram peso corporal, parâmetros metabólicos e hormonais, pressão arterial sistólica e teste oral de tolerância à glicose. O nível de significância foi de 5%. O ciclo de dietas hipercalóricas promoveu aumento de peso e gordura corporal, pressão arterial sistólica e níveis séricos de glicose, triacilglicerol, insulina e leptina no grupo HD. Além disso, o grupo HD apresentou tolerância à glicose diminuída. em conclusão, os resultados deste estudo mostram que o ciclo de dietas hipercalóricas promove obesidade e exibe várias características comumente associadas com a obesidade humana, como aumento da pressão arterial, resistência à insulina, hiperglicemia, hiperinsulinemia, hiperleptinemia e dislipidemia.

Bactérias coliformes totais e coliformes de origem fecal em águas usadas na dessedentação de animais

Foram colhidas 105 amostras de água sendo 44 de mananciais e de 61 bebedouros, a partir das quais foram realizadas as determinações dos NMP (Número Mais Provável) de bactérias coliformes totais e fecais. de acordo com a Portaria GM/0013 de 15 de janeiro de 1976, da Secretaria Especial do Meio Ambiente do Ministério do Interior, os 44 mananciais revelaram-se dentro dos parâmetros estabelecidos. Embora não haja referência com relação a bebedouros, aplicando-se os valores estabelecidos para mananciais, das 61 amostras estudadas, somente 6 (9,8%) não poderiam ser usadas para a dessedentação de animais. Das 105 amostras analisadas, verificou-se que as condições sanitárias revelaram-se não satisfatórias em 6 (5,7%) amostras de bebedouros quanto a coliformes fecais, sendo 3 (2,8%) também com relação a coliformes totais.