957 resultados para floating


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Members of the subfamily Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as preferential sites for primary replication. However, bovine herpesvirus 5 (BoHV5) is neurotropic and neuroinvasive and responsible for meningoencephalitis in cattle and in animal models. A related virus, BoHV1 has also been occasionally implicated in natural cases of neurological infection and disease in cattle. The aim of the present study was to assess the in vitro effects of BoHV1 and BoHV5 replication in neuron-like cells. Overall, cytopathic effects, consisting of floating rounded cells, giant cells and monolayer lysis, induced by both viruses at 48 h postinfection (p.i.) resulted in a loss of cell viability and high virus titres (r = 0.978). The BoHV1 Cooper strain produced the lowest titres in neuron-like cells, although viral DNA was detected in infected cells during all experiments. Virus replication in infected cells was demonstrated by immunocytochemistry, flow cytometry and qPCR assays. BoHV antigens were better visualized at 48 h p.i. and flow cytometry analysis showed that SV56/90 and Los Angeles antigens were present at higher levels. In spite of the fact that BoHV titres dropped at 48 h p.i, viral DNA remained detectable until 120 h p.i. Sensitive TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) and annexin V assays were used to identify apoptosis. BoHV5 induced death in approximately 50 % of cells within 24 h p.i., similar to what has been observed for BoHV1 Los Angeles. Infection with the BoHV1 Cooper strain resulted in 26.37 % of cells being in the early stages of apoptosis; 63.69 % of infected cells were considered viable. Modulation of mitochondrial function, as measured by mitochondrial membrane depolarization, was synchronous with the virus replication cycle, cell viability and virus titres at 48 h p.i. Our results indicate that apoptosis plays an important role in preventing neuronal death and provides a bovine-derived in vitro system to study herpesvirus-neuron interactions.

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Introduction: The evaluation of the perception of graduates is an essential strategy to support the political project educational in universities. Objective: This study aimed to verify the perception of dentists which are working in the labor market, about the difficulties of professional insertion after the graduation, positives and negatives aspects and suggestions regarding the training received. Methodology: This is a qualitative survey conducted with graduates of the years 2000 to 2010, of dentistry course from a Brazilian Public University. A self-administered questionnaire was sent by mail/email to all graduates in the period. The data were transcribed and subjected to content analysis, divided into pre-analysis, characterized by carefully reading floating, material exploration, guidelines for finding the analysis itself. Result: The categories related to the difficulties in early life were: getting a job/workplace, adequate working conditions and low pay, insecurity and confrontation obtained the academic and lack of administrative experience. The perceived positive aspects for the training were: good infrastructure and reputation of the university, integrating teaching-research, teacher-student relationship. The negative aspects comprised the following categories: administrative preparation, lack of integration between content theoretical/ practical, concepts used in the labor market. The suggestions presented are consistent with the difficulties to the beginning of professional life and the negative aspects. Conclusion: The professionals have faced the saturation of the labor market and different reality from those found in academic life. Despite the positive aspects, graduates suggest changes in the course conducted.

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The status epilepticus (SE) is characterized by a series of crises which occur without recovery of consciousness or a single seizure lasting more than 30 minutes and can damage the central nervous system and systemic. The duration and frequency of attacks are directly related to patient prognosis. Whether seizures occur often and are longer, increase the risk of neurological and systemic complications. Child population has a higher frequency of seizures and it is associated with a lower threshold of immature brain to trigger these episodes. Aim: To determine the safest drugs prescribed for children in SE, their doses and schedules. A bibliographic survey was performed in electronic databases. Methods: The scientific health descriptors used for search was: “status epilecticus” and “anticonvulsivants” and “child”. Results: This strategy identified 396 manuscripts, of whom four were considered eligible for the study, after the assessment by floating reading and criteria. Of these, two were randomized trials and two descriptive. The studies address the use of oral midazolam, rectal diazepam and intravenous lorazepam. Conclusions: All investigated drugs were effective in treat in status epilepticus. Lorazepam is highlight, since it has fewer secondary effects and, as an alternative to the intravenous, oral midazolam. However, there is a need for further studies to demonstrate the efficacy and safety in the use of drugs in children.

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Pós-graduação em Agronegócio e Desenvolvimento - Tupã

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In this paper are discussed the main project stages of an atmospheric storage tank with floating roof. It emphasizes the calculations of the bottom, the shell and the floating roof, discussing its stages and conditions, based on the technical standards of the project. The storage tanks are static equipment working under low pressure and has an essential importance for the operation of a process plant. This paper has the purpose of obtaining the dimensions and materials of each component of the tank through the calculations and prove them with results obtained by numerical simulation by finite elements with the help of Autodesk Inventor software, consolidating the project

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In this paper are discussed the main project stages of an atmospheric storage tank with floating roof. It emphasizes the calculations of the bottom, the shell and the floating roof, discussing its stages and conditions, based on the technical standards of the project. The storage tanks are static equipment working under low pressure and has an essential importance for the operation of a process plant. This paper has the purpose of obtaining the dimensions and materials of each component of the tank through the calculations and prove them with results obtained by numerical simulation by finite elements with the help of Autodesk Inventor software, consolidating the project

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An analysis methodology is presented as well as a comparison of results obtained from vortex-induced motion (VIM) model tests of the MonoGoM platform, a monocolumn floating unit designed for the Gulf of Mexico. The choice of scale between the model and the platform in which the tests took place was a very important issue that took into account the basin dimensions and mooring design. The tests were performed in three different basins: the IPT Towing Tank in Brazil (Sept. 2005), the NMRI Model Ship Experimental Towing Tank in Japan (Mar. 2007), and the NMRI Experimental Tank in Japan (Jun. 2008). The purpose is to discuss the most relevant issues regarding the concept, execution, and procedures to comparatively analyze the results obtained from VIM model tests, such as characteristic motion amplitudes, motion periods, and forces. The results pointed out the importance of considering the 2DOF in the model tests, i.e., the coexistence of the motions in both in-line and transverse directions. The approach employed in the tests was designed to build a reliable data set for comparison with theoretical and numerical models for VIM prediction, especially that of monocolumn platforms. [DOI: 10.1115/1.4003494]

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Risers are flexible multilayered pipes formed by an inner flexible metal structure surrounded by polymer layers and spiral wound steel ligaments, also known as armor wires. Since these risers are used to link subsea pipelines to floating oil and gas production installations, and their failure could produce catastrophic consequences, some methods have been proposed to monitor the armor integrity. However, until now there is no practical method that allows the automatic non-destructive detection of individual armor wire rupture. In this work we show a method using magnetic Barkhausen noise that has shown high efficiency in the detection of armor wire rupture. The results are examined under the cyclic and static load conditions of the riser. This work also analyzes the theory behind the singular dependence of the magnetic Barkhausen noise on the applied tension in riser armor wires.

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Vortex-induced motions (VIM) of floating structures are very relevant for the design of mooring and riser systems. In the design phase, spar and monocolumn VIM behavior, as well as semisubmersible and tension leg platform flow-induced motions, is studied and evaluated. This paper provides a checklist of topics and evidence from a number of sources to justify the selection that should be considered when designing spars or monocolumn platforms regarding the VIM phenomenon. An overview of the influential aspects of the VIM is presented such as heading, external appendages of the hull, concomitant presence of waves and currents, motion suppressor, draft condition (immersed portion of the hull), and external damping due to the presence of risers. Previous works concerning the VIM studies on spar and monocolumn platforms are also addressed. Whenever possible, the results of experiments from diverse authors on this matter are presented and compared. [DOI: 10.1115/1.4003698]

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Robust analysis of vector fields has been established as an important tool for deriving insights from the complex systems these fields model. Traditional analysis and visualization techniques rely primarily on computing streamlines through numerical integration. The inherent numerical errors of such approaches are usually ignored, leading to inconsistencies that cause unreliable visualizations and can ultimately prevent in-depth analysis. We propose a new representation for vector fields on surfaces that replaces numerical integration through triangles with maps from the triangle boundaries to themselves. This representation, called edge maps, permits a concise description of flow behaviors and is equivalent to computing all possible streamlines at a user defined error threshold. Independent of this error streamlines computed using edge maps are guaranteed to be consistent up to floating point precision, enabling the stable extraction of features such as the topological skeleton. Furthermore, our representation explicitly stores spatial and temporal errors which we use to produce more informative visualizations. This work describes the construction of edge maps, the error quantification, and a refinement procedure to adhere to a user defined error bound. Finally, we introduce new visualizations using the additional information provided by edge maps to indicate the uncertainty involved in computing streamlines and topological structures.

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We propose a slow-wave MEMS phase shifter that can be fabricated using the CMOS back-end and an additional maskless post-process etch. The tunable phase shifter concept is formed by a conventional slow-wave transmission line. The metallic ribbons that form the patterned floating shield of this type of structure are released to allow motion when a control voltage is applied, which changes the characteristic impedance and the phase velocity. For this device a quality factor greater than 40 can be maintained, resulting in a figure of merit on the order of 0.7 dB/360 degrees and a total area smaller than 0.14 mm(2) for a 60-GHz working frequency. (C) 2011 Elsevier B.V. All rights reserved.