Estudo da densidade de corrente crítica para reversão da magnetização de nanoelementos ferromagnéticos

The discovery that a spin-polarized current is capable of exerting a torque in a ferromagnetic material, through spin transfer, might provide the development of new technological devices that store information via the direction of magnetization. The reduction of current density to revert the magnetization is a primary issue to potential applications on non volatile random access memories (MRAM). We report a theorical study of the dipolar and shape effects on the critical current density for reversal of magnetization, via spin transfer torque (STT), on ferromagnetic nanoelements. The nanostructured system consists on a reference layer, in which the current will be spin-polarized, and a free layer of magnetization reversal. We observed considerable changes on the critical current density as a function of the element’s reversion layer thickness (t = 1.0 nm, 1.5 nm, 2.0 nm e 2.5 nm) and geometry (circular and elliptical), the material kind of the system free layer (Iron and Permalloy) and according to the orientation of the magnetization and the spin polarization with the major axis. We show that the critical current density may be reduced about 50% by reducing the Fe free layer thickness and around 75% when we change the saturation magnetization of circular nanoelements with 2.5 nm of thickness. We still observed a reduction as much as 90% on the current density of reversion for thin nanoelements magnetized along the minor axis direction, using in-plane spin polarization parallel to the magnetization.

Estudo de métodos de tratamento de efluentes (troca iônica e eletroquímico) separados e sequenciais para eliminação de derivados de petróleo

The produce of waste and the amount of the water produced coming from activities of petroleum production and extraction has been a biggest challenge for oil companies with respect to environmental compliance due to toxicity. The discard or the reuse this effluent containing organic compounds as BTEX (benzene, toluene, ethylbenzene and xylene) can cause serious environmental and human health problems. Thus, the objective this paper was study the performance of two process (separately and sequential) in one synthetic effluent for the benzene, toluene and xylene removal (volatile hydrocarbons presents in the produced water) through of electrochemical treatment using Ti/Pt electrode and exchange resin ionic used in the adsorption process. The synthetic solution of BTX was prepared with concentration of 22,8 mg L-1, 9,7 mg L-1 e 9,0 mg L-1, respectively, in Na2SO4 0,1 mol L-1. The experiments was developed in batch with 0.3 L of solution at 25ºC. The electrochemical oxidation process was accomplished with a Ti/Pt electrode with different current density (J = 10, 20 e 30 mA.cm-2). In the adsorption process, we used an ionic exchange resin (Purolite MB 478), using different amounts of mass (2,5, 5 and 10 g). To verify the process of technics in the sequential treatment, was fixed the current density at 10 mA cm-2 and the resin weight was 2.5 g. Analysis of UV-VIS spectrophotometry, chemical oxygen demand (COD) and gas chromatography with selective photoionization detector (PID) and flame ionization (FID), confirmed the high efficiency in the removal of organic compounds after treatment. It was found that the electrochemical process (separate and sequential) is more efficient than absorption, reaching values of COD removal exceeding 70%, confirmed by the study of the cyclic voltammetry and polarization curves. While the adsorption (separately), the COD removal did not exceed 25,8%, due to interactions resin. However, the sequential process (electrochemical oxidation and adsorption) proved to be a suitable alternative, efficient and cost-effectiveness for the treatment of effluents petrochemical.

Avaliação do tratamento eletroquímico (direto e indireto) como alternativa de degradação do corante azul de metileno

Textile industry has been a cause of environmental pollution, mainly due to the generation of large volumes of waste containing high organic loading and intense color. In this context, this study evaluated the electrochemical degradation of synthetic effluents from textile industry containing Methylene Blue (AM) dye, using Ti/IrO2-Ta2O5 and Ti/Pt anodes, by direct and indirect (active chlorine) electrooxidation. We evaluated the influence of applied current density (20, 40 and 60 mA/cm2 ), and the presence of different concentrations of electrolyte (NaCl and Na2SO4), as well as the neutral and alkaline pH media. The electrochemical treatment was conducted in a continuous flow reactor, in which the electrolysis time of the AM 100 ppm was 6 hours. The performance of electrochemical process was evaluated by UV-vis spectrophotometry, chemical oxygen demand (COD) and total organic carbon (TOC). The results showed that with increasing current density, it was possible to obtain 100 % of color removal at Ti/IrO2-Ta2O5 and Ti/Pt electrodes. Regarding the color removal efficiency, increasing the concentration of electrolyte promotes a higher percentage of removal using 0,02 M Na2SO4 and 0,017 M NaCl. Concerning to the aqueous medium, the best color removal results were obtained in alkaline medium using Ti/Pt. In terms of organic matter, 86 % was achieved in neutral pH medium for Ti/Pt; while a 30 % in an alkaline medium. To understand the electrochemical behavior due to the oxygen evolution reaction, polarization curves were registered, determining that the presence of NaCl in the solution favored the production of active chlorine species. The best results in energy consumption and cost were obtained by applying lower current density (20 mA/cm2 ) in 6 hours of electrolysis.

O processo de elaboração do plano de preservação do conjunto urbanístico de Brasília: uma representação conceitual da política de preservação urbana no DF

This research aims to provide a reflection on the preservation practices of Brasilia as Cultural Heritage in four analytical/political dimensions: conceptual, urban, political-institutional and legal. In order to do that, the preparation process for the Plan for the Preservation of Brasilia Urban Set (PPCUB) was taken as research object. This preservation plan is representative of the context which determines the relation between goals and preservation practices in the social production process of urban space. Designed by Lucio Costa in 1957, Brasilia received the Cultural Heritage title 27 years later, in 1987. It was recognized a World Heritage Site by the United Nations Educational, Scientific and Cultural (UNESCO) in the same year as it "represents a unique artistic piece of work, a masterpiece of creative genius"; and "an outstanding example of a type of construction or architectural compound that illustrates a significant stage in history" (SILVA, 2003). Brasilia’s urban conception, also recognised in the district and federal levels, gives prominence to the 'urban scales' – monumental, residential, gregarious and bucolic – as the main aspects to be preserved. Despite being an undoubted representative, Brasilia seemingly displays a contradiction. On the one hand, the essential value of the city’s urban design is acknowledged as cultural heritage at international, national and district levels. On the other hand, numerous ways of urban interventions disregard the principles of that conception. In 2012, the international Monitoring Report raised some issues which highlight the following main needs: primary need for clear definition of the urban scales’ characteristics and boundaries; definition of a legal framework that conciliates national and district laws of occupation and use of land; creation of inter-sectors executive authority with both decision-making and financial autonomy; and promotion of heritage educational programs. This report also proposes "to cancel the current process of approval conducted by PPCUB and establish a formal consultation process through a committee made up by GDF and IPHAN, which will enable the active participation of University of Brasilia, the Architects Association, ICOMOS and local organizations" (SEDHAB, 2010). Already in its drafting process, the international recommendations evidence that preserving Brasilia’s urban design conception is not among the goals to be achieved. Thus, this research highlights that the intentional nature of PPCUB’s plans does little towards realizing the current proposals.

Produção e estudo de multicamadas magnéticas em substrato flexível

Magnetic multilayers are the support for the production of spintronic devices, representing great possibilities for miniaturized electronics industry. having the control to produce devices as well as their physical properties from simple multilayer films to highly complex at the atomic scale is a fundamental need for progress in this area, in recent years has highlighted the production of organic and flexible spintronic devices. Because of this trend, the objective of this work was to produce magnetic multilayers deposited on flexible substrate using magnetron sputtering dc technique. Three sets of samples were prepared. The first set composed of the trilayer type CoFe=Cu(t)=CoFe with different thickness of the metallic spacer. The second set consists of two multilayer subgroups, CoFe=Cu in the presence of IrMn layer as a buffer and the next multilayer as cap layer. The third set consisting of non-magnetostrictive multilayer permalloy (Py=Ta and Py=Ag) on flexible substrate and glass. The magnetic properties, were investigated by magnetometry measurements, ferromagnetic resonance and magnetoimpedance (MI), measurements were carried out at room temperature with the magnetic field always applied on the sample plane. For structural analysis, the diffraction X-ray was used. The results of the trilayer showed a high uniaxial anisotropy field for the sample with a spacer of 4.2 nm. For the multilayer in the presence of IrMn layer as the buffer, the study of static and dynamic magnetic properties showed isotropic behavior. For the multilayer in the presence of IrMn layer as a cap, the results of static magnetic properties of the magnetic behavior exhibited a spin valve structure type. However there was a disagreement with results of ferromagnetic resonance measurements, which was justified by the contribution of the unstable and stable grain to the rotatable anisotropy and Exchange bias in ferromagneticantiferromagnetic interface. The third serie of samples showed similar results behavior for the MI Ag multilayers spacer in both substrates. There are also significant MI changes with the Ta spacer, possible associated with the compressive stress on the flexible substrate sample.

A presença de homens docentes na educação infantil: lugares (des)ocupados

A partir da abordagem investigativa da multirreferencialidade, com base em Jacques Ardoíno, e tendo como lentes teóricas os conceitos de cultura e de gênero, discutidos respectivamente por Victor Hell, Stuart Hall, Alfredo Veiga-Neto, e Joan Scott, Guacira Lopes Louro, Dagmar Meyer, este estudo tem como objetivo analisar as implicações da presença de homens docentes na Educação Infantil, problematizando as relações de gênero a partir de um amálgama de atravessamentos sociais, históricos e culturais. Para tanto, foram realizadas entrevistas semi-estruturadas com dois homens que exercem a função de educador infantil na rede pública de ensino do Município de Natal/RN/Brasil. A análise das entrevistas possibilitou a organização dos dados em quatro focos de discussão. O primeiro deles refere-se às figuras masculinas (re)produzidas no cotidiano da Educação Infantil, cujas representações discursivas constroem o homem como “perigoso”, “poderoso” e “respeitado”, ajudando a perpetuar os vínculos histórica e culturalmente estabelecidos entre a figura masculina e as imagens de autoridade, liderança, comando, ao mesmo tempo em que reforça o suposto caráter natural das relações entre a figura feminina e o cuidado infantil. O segundo foco de discussão trata da polarização entre os elementos do binômio masculino/feminino, ativado constantemente nas relações que se estabelecem entre homens e mulheres no cotidiano da Educação Infantil, tornando as instituições desta etapa de ensino um local de concretização dos mais diversos significados de gênero. O terceiro foco de discussão problematiza a necessidade de afirmação da heterossexualidade dos educadores infantis entrevistados, destacando-se a associação (geralmente feita por gestores, por colegas de trabalho e pelas famílias das crianças) entre a heterossexualidade masculina e o adequado exercício da função docente. O quarto e último foco de discussão trata das lacunas da formação inicial e continuada de educadores infantis, enfatizando que, de modo geral, os cursos que formam professores para atuar em instituições de Educação Infantil tendem a desconsiderar a inserção profissional de homens nessa etapa de ensino. Assim, a Dissertação que ora apresentamos tem como escopo a desnaturalização de estereótipos de gênero no cotidiano da Educação Infantil, entendendo que este é também um contexto muito profícuo para o exercício de resistência às imagens cultural e historicamente associadas a homens e mulheres.

Influência da pressão e da vazão de concentrado sobre o desempenho de ultrafiltração no pós-tratamento de esgotos

Despite the numerous advantages resulting from the use of membrane filters technology, intrinsic limitations fouling process become relevant to its applicability. The control of operating conditions is an important tool to mitigate fouling and achieve good levels of efficiency. In this sense, the objective of this study was to investigate the effect of transmembrane pressure and concentrate flow in the performance of ultrafiltration, applied to the post-treatment of domestic sewage. The process was evaluated and optimized by varying the pressure (0.5 and 1.5 bar) and the concentrate flow (300 and 600 L/h), using a 22 factorial design, in order to investigate the effects on the permeate flow and quality of effluents generated at each operating condition. We evaluated the following quality indicators for permeate: pH, electrical conductivity, total suspended solids, turbidity, calcium and Chemical Oxygen Demand (COD). In all tests, we observed marked reduction in the permeate flux at the early stages, followed by a slow decline that lasted until it reaches a relatively constant level, around 120 minutes of filtration. The increased pressure resulted in a higher initial permeate flux, but the decrease of the flow with time is greater for tests at higher pressure, indicating a more pronounced fouling process. On the other hand, increasing the concentrate flow resulted in a slower decline in permeate flux with the filtration time. Regarding the quality of permeate, the transmembrane pressure of 0,5 bar was the one that allowed better results, and was statistically confirmed through the two-way ANOVA test with repeated measures, significant effect of pressure on the turbidity of the permeate. The concentrate flow, in turn, showed no significant influence on any of the quality parameters. Thus, we conclude that, from an economic and environmental point of view, it is more interesting to operate ultrafiltration membrane system with a lower concentrate flow associated with a low transmembrane pressure, since under these conditions will produce less waste, and the permeate will present lower concentrations of the analyzed constituent, especially lower turbidity.

Estudo da produção eletroquímica de espécies de cloro ativo para tratamento de efluentes sintéticos

The treatment of wastewater is essential to human health. One of the most important steps is the disinfection treatment which uses chlorine to eliminate bacteria as required by environmental agencies. However, the identification of potentially toxic byproducts generated by this method, such as trihalomethanes, has stimulated the development of new alternative disinfection technologies. Among them, heterogeneous photocatalysis, TiO2 photocatalysis and electrochemical disinfection are considered suitable alternatives to the chlorination method. Thus, the present dissertation analyzes the evolution of active chlorine species in a synthetic NaCl solution and it is tested to treat a synthetic solution of the dye Reactive Blue 19 using boron-doped diamond (BDD) and ruthenium oxide (Ti/Ru0.3Ti0.7O2) as anodes. The indirect electrochemical process was discussed in terms of mineralization of the total organic load and percentage of color removal in order to evaluate the applicability of electrochemical technology. Electrochemical experiments were carried out with different current densities (25, 50 and 75 mA.cm-2) during 120 minutes. On the other hand, other important parameter in this study was the influence of the proportion sp3/sp2 on BDD anode on the performance of the evolution of active chlorine species which was investigated by electrolytic techniques (linear polarization), with the intention of determining the related training oxidizing species and consumption energy to chemical or electrochemical reactions. From the results, it can be noted that the BDD electrode showed better efficiency throughout the electrochemical process.

Estudo da produção eletroquímica de espécies de cloro ativo para tratamento de efluentes sintéticos

The treatment of wastewater is essential to human health. One of the most important steps is the disinfection treatment which uses chlorine to eliminate bacteria as required by environmental agencies. However, the identification of potentially toxic byproducts generated by this method, such as trihalomethanes, has stimulated the development of new alternative disinfection technologies. Among them, heterogeneous photocatalysis, TiO2 photocatalysis and electrochemical disinfection are considered suitable alternatives to the chlorination method. Thus, the present dissertation analyzes the evolution of active chlorine species in a synthetic NaCl solution and it is tested to treat a synthetic solution of the dye Reactive Blue 19 using boron-doped diamond (BDD) and ruthenium oxide (Ti/Ru0.3Ti0.7O2) as anodes. The indirect electrochemical process was discussed in terms of mineralization of the total organic load and percentage of color removal in order to evaluate the applicability of electrochemical technology. Electrochemical experiments were carried out with different current densities (25, 50 and 75 mA.cm-2) during 120 minutes. On the other hand, other important parameter in this study was the influence of the proportion sp3/sp2 on BDD anode on the performance of the evolution of active chlorine species which was investigated by electrolytic techniques (linear polarization), with the intention of determining the related training oxidizing species and consumption energy to chemical or electrochemical reactions. From the results, it can be noted that the BDD electrode showed better efficiency throughout the electrochemical process.

Determinação do estado fundamental de compostos antiferromagnéticos da série Tb(1-x)Y(x)RhIn(5): aproximação teórico experimental

We present the results of electrical resistivity, magnetic susceptibility, specific heat and x-ray absorption spectroscopy measurements in Tb1−xYxRhIn5 (x = 0.00, 0.15, 0.4.0, 0.50 e 0.70) single crystals. Tb1−xYxRhIn5 is an antiferromagnetic AFM compound with ordering temperature TN ≈ 46 K, the higher TN within the RRhIn5 serie (R : rare earth). We evaluate the physical properties evolution and the supression of the AFM state considering doping and Crystalline Electric Field (CEF) effects on magnetic exchange interaction between Tb3+ magnetic ions. CEF acts like a perturbation potential, breaking the (2J + 1) multiplet s degeneracy. Also, we studied linear-polarization-dependent soft x-ray absorption at Tb M4 and M5 edges to validate X-ray Absorption Spectroscopy as a complementary technique in determining the rare earth CEF ground state. Samples were grown by the indium excess flux and the experimental data (magnetic susceptibility and specific heat) were adjusted with a mean field model that takes account magnetic exchange interaction between first neighbors and CEF effects. XAS experiments were carried on Total Electron Yield mode at Laborat´onio Nacional de Luz S´ıncrotron, Campinas. We measured X-ray absorption at Tb M4,5 edges with incident polarized X-ray beam parallel and perpendicular to c-axis (E || c e E ⊥ c). The mean field model simulates the mean behavior of the whole system and, due to many independent parameters, gives a non unique CEF scheme. XAS is site- and elemental- specific technique and gained the scientific community s attention as complementary technique in determining CEF ground state in rare earth based compounds. In this work we wil discuss the non conclusive results of XAS technique in TbRhIn5 compounds.

Simulação computacional de materiais bidimensionais: funcionalização de defeitos extensos no grafeno e retenção de contaminantes por argilominerais

In this work are considered two bidimensional systems, with distints chacacteristcs and applicabilitys. Is studied the adsorption of transition metals (MT) Fe, Co, Mn and Ru in extended defects, formed by graphene grain boundaries. First in pristine graphene The hollow site of carbon hexagon, in pristine graphene, are the most stable for MT adsorption. The Dirac cone in eletronic structure of graphene was manteined with the presence of MT. For the considered grain boundaries the MT has a greater stability for absorption in the grain boundaries sites in comparison with pristine graphene. Through the energy barrier values, are observed diffusion chanels for MT localized on the grain boundaries. This diffusion chanels indicate a possible formation of nanolines of MT in graphene. For the first stage of the nanolines, ate observed a better stability for the system with greater MT concentration, due to MT-MT interactions. Also, due to the magnetic moment of the MT, the nanolines show a magnetization. For the most stable configurations the system are metallics, particularly for Fe the band structure indicates an anisotropic spin current. In a second study, are considereted the retention capacity for metallic contaminants Cd and Hg in clayminerals, kaolinite (KAO) and montmorillonite (MMT). Through the adsorption energies of contaminantes in the clayminerals, was observed a increase in stability with the increase of contaminants concentration, due to the interaction Cd-Cd and Hg-Hg. Also, was observed that KAO has a strong interaction beteween monolayers than MMT. In this sence, for the adsoption process of contaminantes in the natural form of KAO and MMT, the latter has a better retention capacity, due to the small net work for contaminant intercalation. However, when the modification of clayminerals, with molecules that increase the spacing between monolayers, exist a optimal condition, which the contaminant absorption are more stable in KAO system than in MMT. In the Langmuir adsorption model for the clayminerals in the optimal monolayer spacing, the retention capacity for Cd and Hg in KAO system are 21% greater than in MMT system. Also, for the X-ray Absorption Near Edge Spectroscopy (XANES) for the K edge of Cd and Hg, are found a positive shift of absorption edge with the decreasing of monolayer spacing. This result indicates a possible way to determine the concentration of adsorbed contaminats in relation to unabsorbed ones, from the decomposition of experimental XANES in the obteined spectras.

Centrais de Compras e de Serviços no Setor de Farmácias do Brasil: Alternativa para a Sobrevivência das Farmácias Independentes Face às Grandes Redes

No setor de farmácias brasileiro, composto por mais de 55.000 unidades, observa-se uma polarização entre as grandes cadeias, que são caracterizadas por gestão profissionalizada e escala de compra, e os varejistas independentes. As farmácias independentes encontraram no associativismo, por meio das centrais de negócios, uma alternativa para competir com as grandes redes. Esta pesquisa, realizada junto a 426 gestores de farmácias que operam em associações, identificou, por meio de análise fatorial exploratória, as principais contribuições das associações para a competitividade face às redes: atendimento; imagem da rede; compras conjuntas; nível de informação; suporte operacional e treinamento. O fator ‘compras conjuntas’, relacionado à escala de compra e que está na origem das associações em geral, figura em terceiro plano, suplantado por outros serviços supridos, o que caracteriza a evolução para o conceito das centrais de serviços. Os resultados evidenciaram que as associações possibilitam melhoria da competitividade das farmácias anteriormente independentes, segundo seus gestores.

(Des)igualdades de Género nas Organizações de Trabalho: o caso de uma autarquia local

Em Portugal, cerca de duas centenas de organizações estão empenhadas a criar e implementar Planos para a igualdade de homens e mulheres. Entre elas estão empresas públicas e privadas, autarquias, associações empresariais, organizações do setor terciário, associações de desenvolvimento, universidades, ministérios e outros departamentos governativos. A pesquisa que aqui se expõe inscreveu-se num projeto mais amplo de diagnóstico em realização na Câmara Municipal da Lousã, que se encontra a promover um Plano Municipal para a Igualdade. Nesse âmbito mostrou-se necessário fazer um diagnóstico interno à organização com uma perspetiva de género, quer em termos de desigualdades de género na composição e situação dos seus efetivos quer de forma a conhecer as representações das pessoas que trabalham na autarquia, em torno do fenómeno da (des)igualdade de mulheres e homens. Para a sua realização foi feita análise estatística a dados fornecidos pela organização, e aplicou-se um inquérito por questionário ao pessoal ao serviço da autarquia. Concluiu-se que estamos na presença do fenómeno da desigualdade em função do sexo na estrutura da organização traduzida pela segregação ocupacional e segregação vertical, sendo os homens que apresentam os maiores níveis de antiguidade, que justificarão, de certa forma, as disparidades salariais existentes. Confirmamos uma dualidade entre o ideal de mulher como mãe e dona-de-casa e o de mulher como pessoa independente, especialmente quando são evocadas as consequências da sua atividade profissional sobre os/as filhos/as e a vida familiar. O papel de cuidar surge assim como o obstáculo a conceções menos tradicionalistas e individualizadoras da identidade feminina que nos remete para uma mudança na atribuição do lugar social de homens e mulheres dentro e fora da família. Sobressai a valorização e enfatização da maternidade e do cuidado das crianças na identidade feminina; ao passo que aos homens se atribui uma maior valorização da atividade profissional. Esta constatação comprova a ambivalência e dicotomia de valores e atitudes entre uma fuga à convencional polarização entre papéis “masculinos” e “femininos” rígida e estereotipadamente definidos. / In Portugal, about two hundred organizations are committed to create and implement plans for men and women’s equality. These include public and private companies, local authorities, business associations, third sector organizations, development associations, universities, ministries and other governmental departments. The research exposed here is part of a broader diagnostic project taking placa in the City Hall, of Lousã which is promoting a Municipal Plan for Equality. In this context it was necessary to make an internal diagnosis to the organization from a perspective of gender, either in terms of gender inequalities in the composition and status of their workers or in order to know the workers representations around the phenomenon of women and men’s equality. To make it passible the organization provided statistical data which was analysed and subsequentaly a questionnaire was applied to the municipality staff. We concluded that there is the phenomenon of gender inequality in the structure of the organization namely occupational/professional segregation and vertical segregation, - men having the highest levels of seniority, which may justify in some way the existing wage gap. The study confirms a duality between the ideal of woman as a mother and housewife and the woman as an independent person, especially when the consequences suggested are related to their professional activity on their children and life family. The role of caring emerges as the obstacle to less traditionalist conceptions of female identity and individualization, which leads us to talk about a change of the social position of men and women within and outside the family. It points out the valuation and emphasizing of motherhood and care in female identity, whereas men are given a greater appreciation of the professional activity. This conclusion confirms the ambivalence and dichotomy of values and attitudes between escape from the conventional polarization between rigid and stereotypically defined roles of "male" and "female".

Dissecting the role of Profilin-1 in microglial cell function: the impact on ROS production

Microglial cells are the resident immune cells of central nervous system (CNS) and the major players in neuroinflammation. These cells are also responsible for surveilling the neuronal microenvironment, and upon injury to the CNS they change their morphology and molecular profile and become activated. Activated status is associated with microglia proliferation, migration to injury foci, increased phagocytic capacity, production and release of reactive oxygen species (ROS), cytokines (pro- or anti-inflammatory) and reactive nitrogen species. Microglia activation is crucial for tissue repair in the healthy brain. However, their chronic activation or deregulation might contribute for the pathophysiology of neurodegenerative diseases. A better understanding of the mechanisms underlying microglial cell activation is important for defining targets and develop appropriate therapeutic strategies to control the chronic activation of microglia. It has been observed an increase in profilin (Pfn) mRNA in microglial cells in the rat hippocampus after unilateral ablation of its major extrinsic input, the entorhinal cortex. This observation suggested that Pfn might be involved in microglia activation. Pfn1 is an actin binding protein that controls assembly and disassembly of actin filaments and is important for several cellular processes, including, motility, cell proliferation and survival. Here, we studied the role of Pfn1 in microglial cell function. For that, we used primary cortical microglial cell cultures and microglial cell lines in which we knocked down Pfn1 expression and assessed the activation status of microglia, based on classical activation markers, such as: phagocytosis, glutamate release, reactive oxygen species (ROS), pro- and anti-inflammatory cytokines. We demonstrated that Pfn1 (i) is more active in hypoxia-challenged microglia, (ii) modulates microglia pro- and anti-inflammatory signatures and (iii) plays a critical role in ROS generation in microglia. Altogether, we conclude that Pfn1 is a key protein for microglia homeostasis, playing an essential role in their activation, regardless the polarization into a pro or anti-inflammatory signature.

Development and characterization of Poly(L-lactic acid) (PLLA) platforms for bone tissue engineering

The development of scaffolds based on biomaterials is a promising strategy for Tissue Engineering and cellular regeneration. This work focuses on Bone Tissue Engineering, the aim is to develop electrically tailored biomaterials with different crystalline and electric features, and study their impacts onto cell biological behavior, so as to predict the materials output in the enhancement of bone tissue regeneration. It is accepted that bone exhibits piezoelectricity, a property that has been proved to be involved in bone growth/repair mechanism regulation. In addition electrical stimulations have been proved to influence bone growth and repair. Piezoelectric materials are therefore widely investigated for a potential use in bone tissue engineering. The main goal is the development of novel strategies to produce and employ piezoelectric biomaterials, with detailed knowledge of mechanisms involved in cell-material interaction. In the current work, poly (L-lactic) acid (PLLA), a synthetic semi-crystalline polymer, exhibiting biodegradibility, biocompatibility and piezoelectricity is studied and proposed as a promoter of enhanced tissue regeneration. PLLA has already been approved for implantation in human body by the Food and Drug Administration (FDA), and at the moment it is being used in several clinical strategies. The present study consists of first preparing films with different degrees of crystallinity and characterizing these PLLA films, in terms of surface and structural properties, and subsequently assessing the behavior of cells in terms of viability, proliferation, morphology and mineralization for each PLLA configuration. PLLA films were prepared using the solvent cast technique and submitted to different thermal treatments in order to obtain different degrees of crystallinity. Those platforms were then electrically poled, positively and negatively, by corona discharge in order to tailor their electrical properties. The cellular assays were conducted by using two different osteoblast cell lines grown directly onto the PLLA films:Human osteoblast Hob, a primary cell culture and Human osteosarcoma MG-63 cell line. This thesis gives also a comprehensive introduction to the area of Bone Tissue Engineering and provides a review of the work done in this field in the past until today, in that same field, including the one related with bone’s piezoelectricity. Then the experimental part deals with the effects of the crystallinity degrees and of the polarization in terms of surface properties and cellular bio assays. Three different degrees of crystallinity, and three different polarization conditions were prepared; which results in 9 different configurations under investigation.