Efeito de quatro tipos de mel na longevidade e reprodução de Catolaccus grandis (Hymenoptera:pteromalidae)

O trabalho teve como objetivo conhecer o melhor tipo de mel em relação à longevidade e fertilidade do parasitóide do bicudo-do-algodoeiro Catolaccus grandis. Adultos recém-emergidos foram transferidos para recipientes plásticos de 500mL, adaptados com tubo para fornecer água e umidade para os insetos. Um casal do parasitóide foi mantido em cada recipiente em câmara climatizada a 25±1°C, UR = 70±10% e fotoperíodo de 14h. O trabalho constou de 4 tratamentos: mel de flor de laranjeira; mel de flores de plantas silvestres; mel de cana-de-açúcar (todos produzidos por Apis mellifera) e mel de flores silvestres produzido por abelha Jataí (Tetragonistica angustula), com 15 repetições cada. Adultos recém-emergidos do parasitóide receberam cinco larvas de Euscepes postfasciatus encapsuladas em parafilm. Três gotículas de mel foram colocadas sobre o parafilm. Calculou-se a longevidade de machos e fêmeas, número de ovos dia-1 fêmea-1 e número total de ovos fêmea-1 e construíram-se as tabelas de fertilidade. As melhores dietas para alimentar adultos de C. grandis foram o mel silvestre e o de laranjeira. A melhor fecundidade foi observada na dieta de mel de laranjeira (101,60 ovos fêmea-1) e os melhores resultados para aumento reprodutivo e populacional deste parasitóide foram obtidos com mel de laranja e com mel silvestre.

Estratégia de produçãp agroindustrial :um estudo dos fatores que afetam a exportação do mel

This thesis approaches the situation of small and medium Brazilian companies facing the exportation process, focusing honey (Apis Mellifera). The world economy has had growing market internationalization, known as globalization. In this context, the exportation has to be faced as a way to minimize the inner market difficulties and increase the levels of quality and productivity to attend the external markets in a competitive way. A company exportation process is not linked to its dimensions, but connected to the commitment with quality, creativity and professionalism. The search for new productive frontiers has benefited Brazilian beekeeper, mainly the northeast ones. Besides, the claim for products that are free of chemical remains, with a bigger aggregate value has increased every day, and the Apismel Company is benefited for having potential to produce organic honey. This paper approaches a case study realized with Sixty-nine beekeeper from Serra do Mel, RN, that make the Apismel Company and it aims to investigate the competitive factors that affects honey exportation. In order to obtain subsidies to execute the objective of this work, it was chosen a questionnaire as instrument of research. The questionnaire was structured from variables that were considered as directions of competition in honey exportation. The results were tabled in Software (Statistics version 11.0). The descriptive, exploiter and Kolmogorov-Smirnov analyses were used to analyze the obtained results. At the end, this work recommends that the Apismel Company promote capacitating courses and technical consultations at the many villages that make the county, to ripen and add efficiency in handling implementation in order to equilibrate production factors and to attract inner and external markets

Agentes polinizadores e produção de grãos em cultura de café arábica cv. “Catuaí Vermelho”

Coffee (Coffea arabica L.) plants had the pollination process of their flowers studied in three coffee producing regions in Brazil: the first in Ribeirao Preto, state of São Paulo, in September of 2004, the second in Carmo do Paraiba, in the state of Minas Gerais, in October of 2006, and, the third, in Altinopolis, state of São Paulo, in September of 2007. In all these locations the coffee variety used was the 'Catuai Vermelho'. The pollinator species visiting the flowers, the frequency with which the flowers were visited by the insects, and the type of material collected by them ( nectar and/or pollen) were determined by counts taking place at the first 10 minutes of each hour from 7:00 AM till 6:00 PM during the months mentioned above. To estimate the fructification percentage, 25 branches were identified with color threads and protected with wire frames covered with a nylon fabric to prevent them from being visited by insects. Another group of 25 branches was similarly identified but remained without any covering so that their flowers were thus freely visited by insects. on the average, the most frequent pollinator was the Africanized bee Apis mellifera (73.7%) followed by the bee Trigona spinipes (14.5%), and Tetragonisca angustula (9.5%). The Africanized bee collected nectar and pollen to almost the same extent: 58.5% of the first and 41.5% of the second. To collect nectar, that species increased its visiting frequency up to 1:00 PM. To collect pollen, A. mellifera visited the flowers up to 1:00 PM with frequency peaks between 8:00 and 9:00 AM. T. spinipes collected pollen (58,5%) and nectar (41.5%). T. angustula collected only pollen. The branches freely visited by insects produced 181 grains on the average whereas those covered with the nylon fabric produced only 81 grains, this meaning a reduction of 55.25% in grain production. Coffee grains produced on the freely visited branches had a mean weight (1.13 g) significantly superior to that of those produced on the covered branches (0.94 g).

Inhibitory effect of propolis and bee venom on the mutagenicity of some direct- and indirect-acting mutagens

The antimutagenic effect of ethanolic extract of propolis (EEP) and honeybee (Apis mellifera) venom, both collected in the State of Sb Paulo, Brazil, was assessed by the Salmonella/microsome assay upon direct- and indirect-acting mutagens. EEP had inhibitory effect (in an ascending order) on the mutagenicity power of daunomycin (TA102), benzo(a)pyrene (TA100), and aflatoxin B-1(TA98) and the venom acted against the mutagenicity of 4-nitro-o-phenylenediamine (TA98) and daunomycin (TA102). (C) 1999 Wiley-Liss, Inc.

Pesticides in the propolis at São Paulo State, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produtos naturais para o controle do ácaro Varroa destructor em abelhas africanizadas

O objetivo deste trabalho foi avaliar os efeitos do ácido oxálico e de óleos essenciais de plantas no controle da infestação pelo ácaro Varroa destructor em colônias de Apis mellifera africanizadas. O experimento foi realizado em delineamento inteiramente casualizado, em 30 colônias, com seis tratamentos e cinco repetições. As colmeias foram tratadas com óleos essenciais de arruda (Ruta graveolens), eucalipto (Eucalyptus spp.) e hortelã (Mentha piperita), além de timol, ácido oxálico e do tratamento controle sem aplicação de produtos. Foram avaliadas a mortalidade de varroas e as taxas de mortalidade de crias e de infestação de varroas em crias e em abelhas adultas, antes e depois da aplicação de cada produto. O ácido oxálico e os óleos de arruda, timol, eucalipto e de hortelã reduziram a mortalidade de crias parasitadas pelo ácaro em 92,1, 83,3, 81,7, 86,4 e 81,3%, respectivamente. O tratamento com ácido oxálico reduziu em 87,4% a infestação de varroas em abelhas adultas. O uso desses produtos é eficiente na redução da mortalidade de crias de A. mellifera parasitadas por V. destructor.

Effect of root surface treatment with propolis and fluoride in delayed tooth replantation in rats

Replantation is an acceptable option for treatment of an avulsed permanent tooth. Nevertheless, an extended extraoral period damages the periodontal ligament and results in external root resorption. The purpose of this study was to assess by histologic and histometric analysis, the influence of propolis 15% (natural resinous substance collected by Apis mellifera bees from various plants) and the fluoride solution used as root surface treatment on the healing process after delayed tooth replantation. Thirty Wistar (Rattus norvegicus albinus) rats were submitted to extraction of their upper right incisor. The teeth were maintained in a dry environment for 60 min. After this, the pulp was extirpated and the papilla, enamel organ and periodontal ligament were removed with scalpel. The teeth were divided into three experimental groups: Group I - teeth immersed in 20 ml of physiologic saline; Group II - teeth immersed in 20 ml of 2% acidulated phosphate sodium fluoride; Group III - teeth immersed in 20 ml of 15% propolis. After 10 min of immersion in the solutions, the root canals were dried and filled with calcium hydroxide paste and the teeth were replanted. The animals were euthanized 60 days after replantation. The results showed that similar external root resorption was seen in the propolis and fluoride groups. Teeth treated with physiologic saline tended to have more inflammatory root resorption compared with those treated with fluoride or propolis. However, the comparative analysis did not reveal statistically significant differences (P > 0.05) between the treatment modalities when used for delayed tooth replantation.

Qualidade dos méis produzidos por Melipona fasciculata Smith da região do cerrado maranhense

The honey of Melipona fasciculata is few known in terms of composition, and therefore generally associated with the characteristics of the honey of Apis mellifera. This study contributes to the knowledge of the physico-chemical characteristics of honey of M. fasciculata of the municipalities of Barra do Corda, Jenipapo dos Vieiras, Fernando Falcão, Carolina and Riachão, in cerrado region from Maranhão. The parameters studied were: moisture, pH, acidity, reducing sugars, apparent sucrose, hydroxymethylfurfural, diastase activity, insoluble solids, ash and color. Some of the observed patterns may conform to the established for A. mellifera, but others must be accompanied by a specific legislation.

Antimicrobial activity of propolis and essential oils and synergism between these natural products

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Behavioral responses evoked in honey bee workers by Dufour gland extracts (Hymenoptera : Apidae)

A series of experiments was performed to assess possible roles of the honey bee Dufour gland secretion. Bioassays with extracts of queen and worker glands from two colonies were made under artificial conditions, in which nestmate and non-nestmate forager workers were tested. The results demonstrate that forager workers display behavioral responses when exposed to Dufour gland extracts of nestmates, but remain indifferent when exposed to non-nestmate extracts. Also, the results demonstrate that forager workers are attracted by virgin queen gland extracts, and repelled by forager worker extracts. The data demonstrate that the Dufour gland secretion is colony- and caste-specific. The attractant remains in the nest. The repellent effect of forager worker extracts is interpreted as an alarm-like pheromone. The attractant effect of virgin queen extracts could be useful in the swarming process to attract scout bees to the eventual founder virgin queen or to attract old foragers to the virgin queen that remains in the nest.

Comparative study of the ultrastructure and secretory dynamic of hypopharyngeal glands in queens, workers and males of Sceptotrigona postica latreille (Hymenoptera, Apinae, Meliponini)

The secretory cycle of hypopharyngeal glands (HPGs) in Scaptotrigona postica resembles that of Apis mellifera: in newly emerged workers the HPGs are in prefunctional state, their maximum development happens in the nurse workers and in forager workers they show signs of reabsorption. In S. postica these glands are also present in queens and males where they are more developed in newly emerged individuals. The ultrastructural features of the HPG secretory cycle in workers of S. postica and A. mellifera are alike: granular endoplasmic reticulum well developed, large secretion masses around the intracellular canaliculus in nurse workers and extensive degenerative structures in forager workers. Then it is suggested that the HPG secrete similar substances in both species. A second secretory cycle seems to occur in early foragers, may be with production of enzymes. The role of the HPGs in queens and males remains unknown but one possibility is enzyme production.

In situ localization of heat-shock proteins and cell death labelling in the salivary gland of acaricide-treated honeybee larvae

The effects of the acaricides, rotenone and oxalic acid (OA) on salivary glands of honeybee larvae were evaluated. Immunohistochemical methods were used to detect cell death and heat-shock protein (HSP70 and 90) localizations. Heat-shock proteins (HSP70 and 90) were localized in the cytoplasm and/or the nuclei of secretory gland cells, both under stress and in normal conditions. In rotenone-treated larvae, there were no changes in the normal level of cell death and also there were no morphological alterations in the secretory cells. In the larvae treated with oxalic acid, the salivary gland showed varying degrees of morphological cellular alteration and an increase in the cell death level. The present data suggest that stress-induced HSP70 might have an antiapoptotic effect while the stress-induced HSP90 might have a chaperone function in the larval salivary glands.

Cephalic salivary glands of two species of advanced eusocial bees (Hymenoptera: Apidae): morphology and secretion

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comparative anatomy of the male reproductive internal organs of 51 species of bees

A anatomia dos órgãos internos do aparelho reprodutor de machos (ARM) adultos e pupas foi comparada em 51 espécies de abelhas, incluindo representantes de seis famílias. Foram obtidos quatro tipos diferentes de ARM. O tipo I está presente em machos das famílias mais basais (Colletidae, Andrenidae e Halictidae) e é caracterizado por três túbulos seminíferos por testículo, o qual é quase totalmente envolvido pela membrana escrotal. O tipo II é um tipo intermediário entre os tipos I e III e está presente em Mellitidae e Megachilidae, como também em alguns Apidae estudados, sendo caracterizado por possuir dutos deferentes pós-vesiculares fora da membrana escrotal e possuir três ou quatro túbulos seminíferos por testículo, exceto Apis mellifera L., a qual possui secundariamente um número aumentado de túbulos. O tipo III foi achado somente nos Apidae estudados e é caracterizado por apresentar os testículos e dutos genitais (exceto o duto deferente pós-vesicular) encapsulados separadamente, as glândulas acessórias são bem desenvolvidas e o duto ejaculador é calibroso, apresentando fissuras em sua parede externa, as quais podem ocorrer também no tipo II. O tipo IV está presente exclusivamente na tribo Meliponini e é caracterizado pela ausência de glândulas acessórias.

Cephalic salivary gland ultrastructure of worker and queen eusocial bees (Hymenoptera, Apidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)