Cancer-cell microsurgery using nonlinear optical endomicroscopy

Near-infrared laser-based microsurgery is promising for noninvasive cancer treatment. To make it a safe technique, a therapeutic process should be controllable and energy efficient, which requires the cancer cells to be identifiable and observable. In this work, for the first time we use a miniaturized nonlinear optical endomicroscope to achieve microtreatment of cancer cells labeled with gold nanorods. Due to the high two-photon-excited photoluminescence of gold nanorods, HeLa cells inside a tissue phantom up to 250 μm deep can be imaged by the nonlinear optical endomicroscope. This facilitates microsurgery of selected cancer cells by inducing instant damage through the necrosis process, or by stopping cell proliferation through the apoptosis process. The results indicate that a combination of nonlinear endomicroscopy with gold nanoparticles is potentially viable for minimally invasive cancer treatment.

User satisfaction in sustainable office buildings : a preliminary study

Energy efficiency was first mandated for commercial buildings in 2006 in Part J of The Building Code of Australia (BCA) and regulators are already implementing increased measures in 2010 (ABCB 2010). Further increases will follow as part of the co-ordinated effort to reduce building related greenhouse gas emissions. The introduction of the Energy Efficiency Disclosure Bill 2010 will establish a national scheme to promote the disclosure of information about the energy efficiency of office buildings as well as further highlighting the need for efficiency. Increased energy efficiency in the form of insulation, energy efficient light fittings, sophisticated Building Management Systems (BMS), micro-generation such as solar and wind turbines all result in measurable quantifiable reductions in operating costs for owners and tenants. However convincing all building owners about the sound business case for adopting sustainability measures has not been fully realised. To-date the adoption of cutting edge sustainable buildings in Australia is restricted to a few industry leaders, such as Investa and ISPT in Victoria for example. Sustainable building owners and tenants often benefit from reduced operating costs during the building lifecycle although the ‘intangible’ effect on businesses (e.g. employee productivity) is uncertain. This aspect has not been accurately quantified and has not been included as part of the measurement of sustainability in buildings.

This study will allow property stakeholders, including government policy-makers and investors/developers, to better understand the optimal type and level of sustainability to be incorporated into the built environment. In addition this knowledge will enable policymakers to make more informed decisions with regards to the likely impact of the legislative measures they propose in respect of sustainability and buildings in The Building Code of Australia (BCA) and other relevant legislation.

Households' perceptions on sustainable home behaviour and improvements in Australia

The consumption of energy in the residential building is a major contributor to Australia's stationary energy greenhouse gas emissions. With the aim of investigating the householders' motivations and perspectives on sustainable home improvements, this study, by using an online survey instrument, collected more than 500 sets of questionnaire data from households in the state of New South Wales, Australia. Through statistical analysis of the data collected, this research has found that construction cost and government incentive were considered as major influence factors on achieving energy efficient residential building development, and the lower bills from reduced energy and water consumption were considered as the most important benefits from the households' perspectives. The research also found that although many households exhibited a high level of awareness or had implemented some sustainability improvements, the total number of potential improvements scored poorly. A suggestion, based on these research findings, is that the government should promote the reasons and benefits for sustainability home improvements that are identified in this research paper, and try to reduce material costs and improve government incentives.

Thermal performance analysis of earth pipe cooling system for subtropical climate

Energy crisis is one of the major obstacles for human development. There are on-going researches to overcome this for a sustainable environment and economy. Passive air cooling system of earth pipe cooling is seen as a viable energy efficient technology for hot and humid subtropical climates. It can be an attractive economical alternative to conventional cooling since there are no compressors or any habitual mechanical unit. It utilizes earth’s near constant underground temperature to cool air for residential, agricultural or industrial uses. This paper reports the thermal performance of earth pipe cooling technology for a hot and humid subtropical climatic zone in Queensland, Australia. A series of pipes buried underground were used in order to increase the cooling performance of the system. To measure the thermal performance, a thermal model was developed for the earth pipe cooling system and simulated using ANSYS Fluent. Data were collected from two modelled rooms built from shipping containers and installed at Central Queensland University, Rockhampton, Australia. The impact of air temperature and velocity on room cooling performance has also been assessed. A significant temperature reduction is seen in this study, which will save energy cost for thermal cooling in buildings.

Springback investigation in roll forming of a V-section

To have fuel efficient vehicles with a lightweight structure, the use of High Strength Steels (HSS) and Advanced High Strength Steels (AHSS) in the body of automobiles is increasing. Roll forming is used widely to form AHSS materials. Roll forming is a continuous process in which a flat strip is shaped to the desired profile by passing through numerous sets of rolls. Formability and springback are two major concerns in the roll forming of AHSS materials. Previous studies have shown that the elastic modulus (Young's modulus) of AHSS materials can change when the material undergoes plastic deformation and the main goal of this study is to numerically investigate the effect of a change in elastic modulus during forming on springback in roll forming. Experimental loading-unloading tests have been performed to obtain the material properties of TRIP 700 steel and incorporate those in the material model used in the numerical simulation of the roll forming process. The finite element simulations were carried out using MSC-Marc and two different element types, a shell element and a solid-shell element, were investigated. The results show that the elastic modulus diminution due to plastic strain increases the springback angle by about 60% in the simple V-section roll forming analyzed in this study. © (2014) Trans Tech Publications, Switzerland.

Novel approach for information discovery in autonomous wireless sensor networks

Autonomous Wireless sensor networks(WSNs) have sensors that are usually deployed randomly to monitor one or more phenomena. They are attractive for information discovery in large-scale data rich environments and can add value to mission–critical applications such as battlefield surveillance and emergency response systems. However, in order to fully exploit these networks for such applications, energy efficient, load balanced and scalable solutions for information discovery are essential. Multi-dimensional autonomous WSNs are deployed in complex environments to sense and collect data relating to multiple attributes (multi-dimensional data). Such networks present unique challenges to data dissemination, data storage of in-network information discovery. In this paper, we propose a novel method for information discovery for multi-dimensional autonomous WSNs which sensors are deployed randomly that can significantly increase network lifetime and minimize query processing latency, resulting in quality of service (QoS) improvements that are of immense benefit to mission–critical applications. We present simulation results to show that the proposed approach to information discovery offers significant improvements on query resolution latency compared with current approaches.

Target coverage through distributed clustering in directional sensor networks

Maximum target coverage with minimum number of sensor nodes, known as an MCMS problem, is an important problem in directional sensor networks (DSNs). For guaranteed coverage and event reporting, the underlying mechanism must ensure that all targets are covered by the sensors and the resulting network is connected. Existing solutions allow individual sensor nodes to determine the sensing direction for maximum target coverage which produces sensing coverage redundancy and much overhead. Gathering nodes into clusters might provide a better solution to this problem. In this paper, we have designed distributed clustering and target coverage algorithms to address the problem in an energy-efficient way. To the best of our knowledge, this is the first work that exploits cluster heads to determine the active sensing nodes and their directions for solving target coverage problems in DSNs. Our extensive simulation study shows that our system outperforms a number of state-of-the-art approaches.

Uma análise energética da cristalização por resfriamento na indústria da cana-de-açúcar

Os mercados dos produtos oriundos da cana-de-açúcar no mundo vêm sofrendo nos últimos anos mudanças estruturais significativas. No cenário brasileiro, houve grande expansão da produção de cana-de-açúcar, impulsionados pela demanda dos carros flex –fuel, além da oportunidade de suprir o volume de açúcar que a União Europeia deixara de colocar no mercado mundial pela eliminação da subvenção aos exportadores de açúcar do bloco. As incertezas dos mercados exigem mudanças de estratégias de negócio, usinas projetadas para produção exclusiva de etanol, alteram sua estratégia para produção conjunta etanol e açúcar. Apesar dos processos comuns para produção de açúcar e etanol, não significa que essa mudança de estratégia seja fácil de ser efetivada, principalmente pelo aspecto energético. Estudos de processo de produção de açúcar mais eficientes energeticamente, com custos menores tem ganhado força na União Europeia, dentre elas a cristalização do açúcar por resfriamento. Vários cenários produtivos inclusive integrados a produção de etanol tem sido desenvolvidos ao longo dos anos. O objetivo do trabalho foi verificar por comparação de cenários, duas tecnologias de cristalização do açúcar (cozimento e resfriamento), em relação ao impacto nos excedentes de energia elétrica quando a produção de açúcar é adicionada a unidades que produzem somente etanol e energia elétrica. Por meio de simulações dos balanços térmicos dos cenários, observou-se uma restrição importante quando adotado a cristalização por cozimento, no que se refere aos volumes de excedentes de energia elétrica, e que pode ser minimizado se adotado o processo de cristalização por resfriamento, podendo assim ser uma alternativa quando se deseja mudar a estratégia do negócio, de produção exclusiva de etanol e energia elétrica para etanol, açúcar e energia elétrica.

Todo Brasileiro merece nível A : habitação multifamiliar para o programa minha casa minha vida energeticamente eficiente

This thesis describes the theoretical, methodological and programmatic proposal for a multifamily residential building located in the urban expansion area of Parnamirim/RN, inserted in the program Minha Casa Minha Vida and level of energy efficiency "A", as the RegulamentoTécnico de Qualidade (RTQ-R/INMETRO) for residential buildings. The development project initially consists of procedures as the study of theoretical, architectural programming and cases studies. With the delimitation of a field solution, situated between the reference and the context, proposals are studied to determine the solution and architectural detailing of the proposal. The architectural program was built based on the method of Problem Seeking (Peña and Parshall, 2001) and research has highlighted aspects of reducing the environmental impact and of the program Minha Casa Minha Vida , among others. The design process was characterized by the incorporation of aspects reviewed and programmed, seeking them compatible and have an economically viable building, socio-spatial quality and energy efficient. The results show that it is possible to obtain a building that meets the constraints of the program that provides housing and energy efficiency level A - and many other environmental qualities and constructive, particularly through architectural design

Produção e avaliação de nanoemulsão catiônica como possível vetor não-viral para pIRES2-EGFP

Gene therapy is based on the transfer of exogenous genetic material into cells or tissues in order to correct, supplement or silencing a particular gene. To achieve this goal, efficient vehicles, viral or non-viral, should be developed. The aim of this work was to produce and evaluate a nanoemulsion system as a possible carrier for no-viral gene therapy able to load a plasmid model (pIRES2-EGFP). The nanoemulsion was produced by the sonication method, after been choose in a pseudo-ternary phase diagram build with 5 % of Captex 355®, 1.2 % of Tween 80®, 0.8 % of Span 80®, 0.16% of stearylamine and water (to 100 %). Measurements of droplet size, polydispersity index (PI), zeta potential, pH and conductivity, were performed to characterize the system. Results showed droplets smaller than 200 nm (PI < 0.2) and zeta potential > 30 mV. The formulation pH was near to 7.0 and conductivity was that expected to oil in water systems (70 to 90 μS/s) A scale up study, the stability of the system and the best sterilization method were also evaluated. We found that the system may be scaled up considering the time of sonication according to the volume produced, filtration was the best sterilization process and nanoemulsions were stable by 180 days at 4 ºC. Once developed, the complexation efficiency of the plasmid (pDNA) by the system was tested by agarose gel electrophoresis retardation assay.. The complexation efficiency increases when stearylamine was incorporated into aqueous phase (from 46 to 115 ng/μL); regarding a contact period (nanoemulsion / pDNA) of at least 2 hours in an ice bath, for complete lipoplex formation. The nanoemulsion showed low toxicity in MRC-5 cells at the usual transfection concentration, 81.49 % of survival was found. So, it can be concluded that a nanoemulsion in which a plasmid model was loaded was achieved. However, further studies concerning transfectation efficiency should be performed to confirm the system as non-viral gene carrier

Very large dielectric constant of highly oriented Pb1-xBaxTiO3 thin films prepared by chemical deposition

Highly (100) oriented Pb0.8Ba0.2TiO3/LaNiO3 structures were grown on LaAlO3(100) substrates by using a wet, soft chemical method and crystallized by the microwave oven technique. The Au/PBT/LaNiO3/LaAlO3 capacitor shows a hysteresis loop with remnant polarization, P-r, of 15 muC/cm(2), and coercive field, E-c, of 47 kV/cm at an applied voltage of 3 V, along with a dielectric constant over 1800. Atomic force microscopy showed that Pb0.8Ba0.2TiO3 is composed of large grains about 300 nm. The experimental results demonstrated that the microwave preparation is rapid, clean, and energy efficient. Therefore, we demonstrated that the combination of the soft chemical method with the microwave process is a promising technique to grow highly oriented thin films with excellent dielectric and ferroelectric properties, which can be used in various integrated device applications. (C) 2004 American Institute of Physics.

Climate change: selected economic dimensions

Includes bibliography

Siderurgia e carvoejamento na Amazônia: drenagem energético-material e pauperização regional

O trabalho analisa a implantação, no último quartel do século XX, na Amazônia oriental brasileira, de indústria voltada à produção de ferro-gusa destinado ao mercado internacional de insumos siderúrgicos. Demonstra que, nos anos 1980, o discurso estatal anunciou as plantas industriais deste segmento como elemento estratégico de modernização econômica da região. Tal postura justificou a concessão de benefícios fiscais, creditícios e de infraestrutura a 22 empresas de siderurgia e metalurgia. No âmbito da dimensão ambiental, o estudo demonstra que a produção de ferro-gusa recorre a processos marcados por baixa eficiência energética e que deles resultou o acesso, sem prudência ecológica, a estoque de biomassa da floresta amazônica, com ampliação da pressão antrópica sobre ela. O estudo evidencia que o plantio de grandes áreas de floresta para produzir carvão vegetal não se concretizou, manteve-se tão-somente como retórica desprovida de base realista. Quanto à dimensão econômica, o estudo indica que as limitações de a indústria de ferro-gusa impulsionar processos de modernização vinculam-se, dentre outros aspectos, ao fato de ela ter na demanda de carvão vegetal o principal elo de articulação com a sociedade e com a economia da região. Tal demanda é suprida por centenas de fornecedores e, por meio dela, a indústria controla a margem de lucro e comprime os custos de produção transferindo custos privados para a sociedade. O estudo demonstra, ainda, que o padrão de atuação deste segmento empresarial vincula-se a condicionamentos econômicos e institucionais: a implantação de florestas energéticas exige investimentos de longo prazo, somados às grandes oscilações no preço do ferro-gusa e a dinâmicas institucionais que abrem possibilidade de se acessar, ilegalmente, biomassa de florestas primárias. Isso levou o segmento a recorrer, historicamente, ao suprimento de carvão vegetal produzido, sobretudo, a partir da biomassa de florestas primárias e não da silvicultura. Com base em tais evidências, o trabalho conclui que a predição estatal de impulso regional modernizante não se materializou. A causa principal foi a inexistência de competências sociais para regular a transformação de matéria e energia em mercadorias, e esta produção teve seus fundamentos marcados pela degradação social e ambiental. Assim, atuou em sentido contrário ao discurso estatal: acelerou a transferência energética, material e de valores para outras regiões. Drenagem energético-material que não foi compensada, nem pela capacidade de a região equilibrar as perdas com importações de produtos, nem pela implementação de dinâmicas eficazes para a industrialização da região.

Estratégia de redução de consumo de energia em redes de sensores sem fio heterogêneas utilizando lógica fuzzy

O avanço nas áreas de comunicação sem fio e microeletrônica permite o desenvolvimento de equipamentos micro sensores com capacidade de monitorar grandes regiões. Formadas por milhares de nós sensores, trabalhando de forma colaborativa, as Redes de Sensores sem Fio apresentam severas restrições de energia, devido à capacidade limitada das baterias dos nós que compõem a rede. O consumo de energia pode ser minimizado, permitindo que apenas alguns nós especiais, chamados de Cluster Head, sejam responsáveis por receber os dados dos nós que formam seu cluster e propagar estes dados para um ponto de coleta denominado Estação Base. A escolha do Cluster Head ideal influencia no aumento do período de estabilidade da rede, maximizando seu tempo de vida útil. A proposta, apresentada nesta dissertação, utiliza Lógica Fuzzy e algoritmo k-means com base em informações centralizadas na Estação Base para eleição do Cluster Head ideal em Redes de Sensores sem Fio heterogêneas. Os critérios usados para seleção do Cluster Head são baseados na centralidade do nó, nível de energia e proximidade para a Estação Base. Esta dissertação apresenta as desvantagens de utilização de informações locais para eleição do líder do cluster e a importância do tratamento discriminatório sobre as discrepâncias energéticas dos nós que formam a rede. Esta proposta é comparada com os algoritmos Low Energy Adaptative Clustering Hierarchy (LEACH) e Distributed energy-efficient clustering algorithm for heterogeneous Wireless sensor networks (DEEC). Esta comparação é feita, utilizando o final do período de estabilidade, como também, o tempo de vida útil da rede.