??El tiempo garantiza el cambio en el profesorado? Estudio de un caso centrado en la evaluaci??n de aprendizajes

Monogr??fico con el t??tulo: " Formaci??n de profesores. Perspectivas de Brasil, Colombia, Espa??a y Portugal"

OpenSearch-geo: the simple standard for geographic web search engines

When publishing information on the web, one expects it to reach all the people that could be interested in. This is mainly achieved with general purpose indexing and search engines like Google which is the most used today. In the particular case of geographic information (GI) domain, exposing content to mainstream search engines is a complex task that needs specific actions. In many occasions it is convenient to provide a web site with a specially tailored search engine. Such is the case for on-line dictionaries (wikipedia, wordreference), stores (amazon, ebay), and generally all those holding thematic databases. Due to proliferation of these engines, A9.com proposed a standard interface called OpenSearch, used by modern web browsers to manage custom search engines. Geographic information can also benefit from the use of specific search engines. We can distinguish between two main approaches in GI retrieval information efforts: Classical OGC standardization on one hand (CSW, WFS filters), which are very complex for the mainstream user, and on the other hand the neogeographer’s approach, usually in the form of specific APIs lacking a common query interface and standard geographic formats. A draft ‘geo’ extension for OpenSearch has been proposed. It adds geographic filtering for queries and recommends a set of simple standard response geographic formats, such as KML, Atom and GeoRSS. This proposal enables standardization while keeping simplicity, thus covering a wide range of use cases, in both OGC and the neogeography paradigms. In this article we will analyze the OpenSearch geo extension in detail and its use cases, demonstrating its applicability to both the SDI and the geoweb. Open source implementations will be presented as well

ICOS Carbon Data Portal

La infraestructura europea ICOS (Integrated Carbon Observation System), tiene como misión proveer de mediciones de gases de efecto invernadero a largo plazo, lo que ha de permitir estudiar el estado actual y comportamiento futuro del ciclo global del carbono. En este contexto, geomati.co ha desarrollado un portal de búsqueda y descarga de datos que integra las mediciones realizadas en los ámbitos terrestre, marítimo y atmosférico, disciplinas que hasta ahora habían gestionado los datos de forma separada. El portal permite hacer búsquedas por múltiples ámbitos geográficos, por rango temporal, por texto libre o por un subconjunto de magnitudes, realizar vistas previas de los datos, y añadir los conjuntos de datos que se crean interesantes a un “carrito” de descargas. En el momento de realizar la descarga de una colección de datos, se le asignará un identificador universal que permitirá referenciarla en eventuales publicaciones, y repetir su descarga en el futuro (de modo que los experimentos publicados sean reproducibles). El portal se apoya en formatos abiertos de uso común en la comunidad científica, como el formato NetCDF para los datos, y en el perfil ISO de CSW, estándar de catalogación y búsqueda propio del ámbito geoespacial. El portal se ha desarrollado partiendo de componentes de software libre existentes, como Thredds Data Server, GeoNetwork Open Source y GeoExt, y su código y documentación quedarán publicados bajo una licencia libre para hacer posible su reutilización en otros proyecto

Tratados con Pueblos o Constituciones de Estados: dilema para América

Los derechos de pueblos indígenas, de pueblos que no forman estados y que son anteriores a ellos en el propio territorio manteniendo cultura propia, han sido reconocidos bajo formas jurídicas distintas, según su relación con la estructura de los Estados nacionales.

Multiculturalismo, derechos humanos y Constitución

Constitucionalmente, Ecuador es un Estado "pluricultural y multiétnico". La participación indígena en el último proceso constituyente, 1998, sostuvo dicho reconocimiento, el cual ha sido rehuido por el Estado ecuatoriano desde la misma independencia. Es que, aunque el reconocimiento de la multiculturalidad se redujera tan sólo a un gesto de la Constitución, ya podría resultar de por sí comprometido.

Procesos: revista ecuatoriana de historia. 17 (SOLO LIBROS/Reseñas)

Presenta las reseñas de los siguientes libros: CHARLES WALKER, DE TUPAC AMARU A GAMARRA: CUZCO y LA FORMACIÓN DEL PERÚ REPUBLICANO, 1780-1840, CENTRO DE ESTUDIOS ANDINOS ""BARTOLOMÉ DE LAS CASAS"", CUZCO, 1999, 314 PP. -- CARMEN DUEÑAS DE ANHALZER, MARQUESES, CACAOTEROS y VECINOS DE PORTOVIEJO, UNIVERSIDAD SAN FRANCISCO DE QUITO, ABYA YALA, QUITO, 1997, 320 PP. -- GABRlELA OSSENBACH y MIGUEL SOMOZA, EDITS., Los MANUALES ESCOLARES COMO FUENTE PARA LA HIST0RIA DE LA EDUCACIÓN EN AMÉRICA LATINA, UNED EDICIONES, MADRlD, 2001, 371 PP. -- CATALINA LEÓN PESÁNTEZ, HISPANOAMÉRICA y SUS PARADOJAS EN EL IDEARlO FILOSÓFICO DE JUAN LEÓN MERA, UNIVERSIDAD ANDINA SIMÓN BOLÍVAR/CORPORACIÓN EDITORA NACIONAL/ABYA-YALA, SERIE MAGISTER, No. 16, QUITO, 2001, 94 PP.

La asimilación de la filosofía útil en Nueva Granada: el caso de José Félix de Restrepo, 1773-1791

Esta tesis investiga la asimilación de la filosofía útil en la Nueva Granada, a través del caso de José Félix de Restrepo, entre 1773 y 1791. En aras de contribuir al estudio de uno de los ámbitos de la Ilustración, el educativo. De tal manera, esta tesis se encuentra compuesta por dos capítulos. El primer capítulo está dedicado a la formación educativa de José Félix de Restrepo en la ciudad de Santa fe. Aquí, tengo en cuenta la tensión que se gestó desde mediados del siglo XVIII entre el canon escolástico y el canon moderno de educación. Tensión que marcó la formación de Restrepo y que lo llevó en su primer periodo como docente a enfrentar múltiples desafíos. Mientras que en el segundo capítulo estudio la estadía de Restrepo en Popayán, luego de haber pasado por un proceso de apropiación de la filosofía útil en Santafé, y la forma en la que durante sus tres periodos de clases logró difundir en sus estudiantes los conocimientos adquiridos en el Colegio de San Bartolomé. Conocimientos que contribuyeron significativamente a que en una ciudad donde no residió el centro de poder, es decir periférica, como Popayán, se fuera estableciendo un “nuevo polo de apropiación del pensamiento experimentalista”.

Nociones de soberanía nacional y libre determinación indígena: pugna y articulaciones de conocimientos en el constitucionalismo de Colombia y Ecuador

La noción de soberanía reflejada en el constitucionalismo de Ecuador y Colombia ha sido construida en base a la idea de superioridad de unos seres humanos sobre otros, ajustándose a las necesidades de las élites políticas, y negando la capacidad de auto gobierno de los pueblos indígenas. Esta negación ha girado en torno a nociones eurocentradas de libertad e igualdad que no encontraron correlato en las prácticas de las sociedades indígenas, y por esta razón estos pueblos han sido inferiorizados y sometidos a procesos de civilización e integración. En esta tesis reviso los antecedentes históricos del lugar jurídico subalternizado que ha sido construido para el indígena en el constitucionalismo de Ecuador y Colombia, y propongo que las asambleas constituyentes de los noventa en ambos países han marcado un punto de inflexión en las políticas de reconocimiento, representando una oportunidad para construir interrelaciones humanas en base a la interculturalidad. Es mi esperanza que poco a poco nuestras sociedades se abran a nuestra verdad histórica y que construyamos sistemas de hacer justicia que no estén enfocados en la noción de soberanía de los estados, sino en la libre determinación de los pueblos.

Gut microbial catabolism of grape seed flavan-3-ols by human faecal microbiota. Targetted analysis of precursor compounds, intermediate metabolites and end-products.

In vitro batch culture fermentations were conducted with grape seed polyphenols and human faecal microbiota, in order to monitor both changes in precursor flavan-3-ols and the formation of microbial-derived metabolites. By the application of UPLC-DAD-ESI-TQ MS, monomers, and dimeric and trimeric procyanidins were shown to be degraded during the first 10 h of fermentation, with notable inter-individual differences being observed between fermentations. This period (10 h) also coincided with the maximum formation of intermediate metabolites, such as 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone and 4-hydroxy-5-(3′,4′-dihydroxyphenyl)-valeric acid, and of several phenolic acids, including 3-(3,4-dihydroxyphenyl)-propionic acid, 3,4-dihydroxyphenylacetic acid, 4-hydroxymandelic acid, and gallic acid (5–10 h maximum formation). Later phases of the incubations (10–48 h) were characterised by the appearance of mono- and non-hydroxylated forms of previous metabolites by dehydroxylation reactions. Of particular interest was the detection of γ-valerolactone, which was seen for the first time as a metabolite from the microbial catabolism of flavan-3-ols. Changes registered during fermentation were finally summarised by a principal component analysis (PCA). Results revealed that 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone was a key metabolite in explaining inter-individual differences and delineating the rate and extent of the microbial catabolism of flavan-3-ols, which could finally affect absorption and bioactivity of these compounds.

In vitro fermentation of a red wine extract by human gut microbiota: changes in microbial groups and formation of phenolic metabolites

An in vitro batch culture fermentation experiment was conducted with fecal inocula from three healthy volunteers in the presence and absence of a red wine extract. Changes in main bacterial groups were determined by FISH during a 48 h fermentation period. The catabolism of main flavonoids (i.e., flavan-3-ols and anthocyanins) and the formation of a wide a range of phenolic microbial metabolites were determined by a targeted UPLC-PAD-ESI-TQ MS method. Statistical analysis revealed that catechol/pyrocatechol, as well as 4-hydroxy-5-(phenyl)-valeric, 3- and 4-hydroxyphenylacetic, phenylacetic, phenylpropionic, and benzoic acids, showed the greatest increases in concentration during fermentation, whereas 5-(3′-hydroxyphenyl)-γ-valerolactone, its open form 4-hydroxy-5-(3′-hydroxyphenyl)-valeric acid, and 3,4-dihydroxyphenylacetic acid represented the largest interindividual variations in the catabolism of red wine polyphenols. Despite these changes, microbial catabolism did not produce significant changes in the main bacterial groups detected, although a slight inhibition of the Clostridium histolyticum group was observed.

In vitro fermentation of grape seed flavan-3-ol fractions by human faecal microbiota: changes in microbial groups and phenolic metabolites

With the aim of investigating the potential of flavan-3-ols to influence the growth of intestinal bacterial groups, we have carried out the in vitro fermentation, with human faecal microbiota, of two purified fractions from grape seed extract (GSE): GSE-M (70% monomers and 28% procyanidins) and GSE-O (21% monomers and 78 % procyanidins). Samples were collected at 0, 5, 10, 24, 30 and 48 h of fermentation for bacterial enumeration by fluorescent in situ hybridization and for analysis of phenolic metabolites. Both GSE-M and GSE-O fractions promoted growth of Lactobacillus/Enterococcus and decrease in the Clostridium histolyticum group during fermentation, although the effects were only statistically significant with GSE-M for Lactobacillus/Enterococcus (at 5 and 10 h of fermentation) and GSE-O for C. histolyticum (at 10 h of fermentation). Main changes in polyphenol catabolism also occurred during the first 10 h of fermentation, however no significant correlation coefficients (P>0.05) were found between changes in microbial populations and precursor flavan-3-ols or microbial metabolites. Together these data suggest that the flavan-3-ol profile of a particular food source could affect the microbiota composition and its catabolic activity, inducing changes that could in turn affect the bioavailability and potential bioactivity of these compounds.

Rescue of gene expression by modified REST decoy oligonucleotides in a cellular model of Huntington's disease

Transcriptional dysfunction is a prominent hallmark of Huntington's disease (HD). Several transcription factors have been implicated in the aetiology of HD progression and one of the most prominent is repressor element 1 (RE1) silencing transcription factor (REST). REST is a global repressor of neuronal gene expression and in the presence of mutant Huntingtin increased nuclear REST levels lead to elevated RE1 occupancy and a concomitant increase in target gene repression, including brain-derived neurotrophic factor. It is of great interest to devise strategies to reverse transcriptional dysregulation caused by increased nuclear REST and determine the consequences in HD. Thus far, such strategies have involved RNAi or mutant REST constructs. Decoys are double-stranded oligodeoxynucleotides corresponding to the DNA-binding element of a transcription factor and act to sequester it, thereby abrogating its transcriptional activity. Here, we report the use of a novel decoy strategy to rescue REST target gene expression in a cellular model of HD. We show that delivery of the decoy in cells expressing mutant Huntingtin leads to its specific interaction with REST, a reduction in REST occupancy of RE1s and rescue of target gene expression, including Bdnf. These data point to an alternative strategy for rebalancing the transcriptional dysregulation in HD.

The role of REST in transcriptional and epigenetic dysregulation in Huntington's disease

Huntington's disease (HD) is a devastating disorder that affects approximately 1 in 10,000 people and is accompanied by neuronal dysfunction and neurodegeneration. HD manifests as a progressive chorea, a decline in mental abilities accompanied by behavioural, emotional and psychiatric problems followed by, dementia, and ultimately, death. The molecular pathology of HD is complex but includes widespread transcriptional dysregulation. Although many transcriptional regulatory molecules have been implicated in the pathogenesis of HD, a growing body of evidence points to the pivotal role of RE1 Silencing Transcription Factor (REST). In HD, REST, translocates from the cytoplasm to the nucleus in neurons resulting in repression of key target genes such as BDNF. Since these original observations, several thousand direct target genes of REST have been identified, including numerous non-coding RNAs including both microRNAs and long non-coding RNAs, several of which are dysregulated in HD. More recently, evidence is emerging that hints at epigenetic abnormalities in HD brain. This in turn, promotes the notion that targeting the epigenetic machinery may be a useful strategy for treatment of some aspects of HD. REST also recruits a host of histone and chromatin modifying activities that can regulate the local epigenetic signature at REST target genes. Collectively, these observations present REST as a hub that coordinates transcriptional, posttranscriptional and epigenetic programmes, many of which are disrupted in HD. We identify several spokes emanating from this REST hub that may represent useful sites to redress REST dysfunction in HD.

Structural role of fluoride in the ion-conducting glass system B(2)O(3)-PbO-LiF studied by single- and double-resonance NMR

The local structure of an ion-conducting glass with nominal composition 50B(2)O(3)-10PbO-40LiF has been investigated by complementary (7)Li, (11)B, (19)F, and (207)Pb single- and double-resonance experiments. The results give insight into the structural role of the lithium fluoride additive in borate glasses: (1) LiF is seen to actively participate in the network transformation process contributing to the conversion of three- into four-coordinate boron units, as shown by (11)B single-resonance as well as by (11)B{(19)F} and (19)F{(11)B} double-resonance experiments. (2) (19)F signal quantification experiments suggest substantial fluoride loss, presumably caused by formation of volatile BF(3). A part of the fluoride remains in the dopant role, possibly in the form of small LiF-like cluster domains, which serve as a mobile ion supply. (3) The extent of lithium-fluorine and lead-fluorine interactions has been characterized by (7)Li{(19)F} and (207)Pb{(19)F} REDOR and SEDOR experiments. On the basis of these results, a quantitative structural description of this system has been developed.

Identidade territorial em unidades de conservação : ponto de apoio para uma análise epistemológica da questão ambiental

Esta dissertação de mestrado consiste em um estudo epistemológico da questão ambiental, baseado na análise das identidades territoriais das populações habitantes de Unidades de Conservação (UC’s). Tais populações geralmente têm um modo de vida peculiar e muito vinculado com o espaço onde vivem. Além disso, elas também têm outras percepções sobre a questão ambiental e os conceitos relacionados. Estas percepções, entretanto, geralmente são subjugadas em nome da tecnocracia presente nos órgãos e instituições que trabalham com a temática ambiental. Neste contexto, o trabalho realiza um levantamento dos impasses e conflitos existentes a partir das considerações acima expostas, e os relaciona com as visões, sobre a questão ambiental, dos diferentes grupos sociais envolvidos (poder público, setor privado, ONG´s, movimentos sociais e populações tradicionais). A esta análise acrescenta uma (re)leitura dos métodos científicos à luz da epistemologia (positivismo, neopositivismo, materialismo histórico e dialético, fenomenologia, pós-modernismo e anarquismo), e o modo como cada um deles entende os conceitos de natureza e ambiente. Após, relaciona estas concepções metodológicas com as visões dos diferentes grupos, identificando a posição mais ligada a cada um deles. Com isso, as UC’s são questionadas a partir de sua base epistêmica, que reflete a matriz de pensamento ocidental moderno, e que por sua vez tende a dicotomizar homem e natureza. Ao final, são propostas outras leituras, baseadas em outras matrizes epistemológicas, para superar os impasses relativos a este viés da questão ambiental.