460 resultados para Blister rust


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Genomic selection (GS) has been used to compute genomic estimated breeding values (GEBV) of individuals; however, it has only been applied to animal and major plant crops due to high costs. Besides, breeding and selection is performed at the family level in some crops. We aimed to study the implementation of genome-wide family selection (GWFS) in two loblolly pine (Pinus taeda L.) populations: i) the breeding population CCLONES composed of 63 families (5-20 individuals per family), phenotyped for four traits (stem diameter, stem rust susceptibility, tree stiffness and lignin content) and genotyped using an Illumina Infinium assay with 4740 polymorphic SNPs, and ii) a simulated population that reproduced the same pedigree as CCLONES, 5000 polymorphic loci and two traits (oligogenic and polygenic). In both populations, phenotypic and genotypic data was pooled at the family level in silico. Phenotypes were averaged across replicates for all the individuals and allele frequency was computed for each SNP. Marker effects were estimated at the individual (GEBV) and family (GEFV) levels with Bayes-B using the package BGLR in R and models were validated using 10-fold cross validations. Predicted ability, computed by correlating phenotypes with GEBV and GEFV, was always higher for GEFV in both populations, even after standardizing GEFV predictions to be comparable to GEBV. Results revealed great potential for using GWFS in breeding programs that select families, such as most outbreeding forage species. A significant drop in genotyping costs as one sample per family is needed would allow the application of GWFS in minor crops.

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This study investigated the physical characteristics of lightweight concrete produced using waste materials as coarse aggregate. The study was inspired by the author’s Peace Corps service in Kilwa, Tanzania. Coconut shell, sisal fiber, and PET plastic were chosen as the test waste products due to their abundance in the area. Two mixes were produced for each waste product and the mix proportions designed for resulting compressive strengths of 3000 and 5000 psi. The proportions were selected based on guidelines for lightweight concrete from the American Concrete Institute. In preparation for mixing, coconut shells were crushed into aggregate no larger than 3/4 inch, sisal fiber was cut into pieces no longer than 3/8 inch, and PET plastic was shredded into 1/4 inch-wide strips no longer than 6 inches. Replicate samples were mixed and then cured for 28 days before they were tested for compressive strength, unit weight, and absorption. The resulting data were compared to ASTM Standards for lightweight concrete masonry units to determine their adequacy. Based on these results, there is potential for coconut shell to be used as coarse aggregate in lightweight concrete. Sisal fiber was unsuccessful in producing the appropriate compressive strength. However, the reduction in spalling of the hardened concrete and the induction of air in the mixes incorporating sisal fiber suggests that it has the potential to improve other characteristics of lightweight concrete. Concrete mixes using PET plastic as aggregate resulted in adequate compressive strengths, but were too dense to be considered ‘lightweight’ concrete. With some adjustments to slightly decrease absorption and unit weight, the PET plastic concrete mixes could be classified as medium weight concrete and, therefore, achieve many of the same benefits as would be seen with lightweight concrete.

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Ribosome profiling (Ribo-seq), a promising technology for exploring ribosome decoding rates, is characterized by the presence of infrequent high peaks in ribosome footprint density and by long alignment gaps. Here, to reduce the impact of data heterogeneity we introduce a simple normalization method, Ribo-seq Unit Step Transformation (RUST). RUST is robust and outperforms other normalization techniques in the presence of heterogeneous noise. We illustrate how RUST can be used for identifying mRNA sequence features that affect ribosome footprint densities globally. We show that a few parameters extracted with RUST are sufficient for predicting experimental densities with high accuracy. Importantly the application of RUST to 30 publicly available Ribo-seq data sets revealed a substantial variation in sequence determinants of ribosome footprint frequencies, questioning the reliability of Ribo-seq as an accurate representation of local ribosome densities without prior quality control. This emphasizes our incomplete understanding of how protocol parameters affect ribosome footprint densities.

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Resumen La historia de los cultivos tropicales en la segunda mitad del siglo XX, es en gran parte, una historia de innovación. Un análisis de esa historia de innovación nos permite vislumbrar la historia ambiental de esos cultivos. Mucha de la innovación de esa época, se hizo para controlar una ola de enfermedades y plagas vegetales sin precedentes. Recuperar la historia de esas enfermedades, nos permitirá, al mismo tiempo, comprender el aumento de la fragilidad ecológica de los principales cultivos en América Central. Este artículo analiza justamente esa problemática a partir del enfoque particular de la historia de la roya del cafeto -Hemileia vastatrix- en América Central, con énfasis en Costa Rica. Abstract The history of tropical crops in the second half of the twentieth century is, in large part, a history of innovation. An analysis of this history of innovation allows us to glimpse the environmental history of these crops. Much of the innovation in this period was done to counter an unprecedented wave of crop diseases and pests. Recovering the history of these diseases, in turn, allows us to understand the increasingly fragile ecology of the main crops in Central America. This article analyzes these themes through a history of the coffee rust - Hemileia vastatrix- in Central America, with particular emphasis on Costa Rica.

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O melhoramento genético do cafeeiro tem dado efetiva contribuição à cafeicultura brasileira em todas as regiões produtoras de café, por meio do desenvolvimento de cultivares com alta capacidade produtiva, aliada a outras caraterísticas de interesse agronômico e tecnológico para os cafeicultores e consumidores de café. Reunir a expressão favorável para os caracteres de interesse em uma cultivar é o objetivo dos programas de melhoramento. Objetivou-se, no presente trabalho, avaliar progênies de cafeeiros do grupo Catucaí, portadoras de caracteres de interesse para o agronegócio café. Dessa forma, foram selecionados genótipos de cafeeiros com características favoráveis de produtividade, tamanho de grãos, vigor vegetativo e resistência à ferrugem. O experimento foi instalado no Campo Experimental de Três Pontas, na EPAMIG SUL,utilizando-se 36 tratamentos, sendo 33 progênies derivadas do cruzamento entre cafeeiros do grupo Icatu e cafeeiros do grupo Catuaí e 3 cultivares testemunhas (Catuaí Amarelo IAC 62, Catucaí Amarelo 2 SL e Icatu Precoce IAC 3282). O delineamento experimental foi o látice quadrado 6 x 6, com 3 repetições e as avaliações foram realizadas durante quatro colheitas (2009/10, 2010/11, 2011/12 e 2012/2013). As características avaliadas foram: produtividade média (sacas ha-1), porcentagem de frutos retidos em peneira 17 e acima, vigor vegetativo, incidência e severidade da ferrugem. Os resultados permitem concluir que os tratamentos apresentaram variabilidade para as características produtividade, tamanho de grãos, incidência e severidade de ferrugem. As progênies H 6-47-10 Cova 3 e H 4-35-11 Cv 10 apresentam produtividade superior às demais progênies e cultivares, com características favoráveis de tamanho de grãos e vigor vegetativo, embora tenham exibido maior infecção de ferrugem. As progênies H MS Cv 13 e H MS Cv 11 merecem destaque, pois além de apresentarem alta produtividade média e elevado vigor vegetativo, pertencem ao grupo de progênies com menor infecção de ferrugem; apesar de estarem posicionadas no segundo agrupamento, em relação ao tamanho de grãos, juntamente com as cultivares comerciais Catuai Amarelo IAC 62 e Catucai Amarelo 2SL.

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BRS Ártico is a common bean cultivar with white grains with international standard size (62 g per 100 seeds), appropriate for cultivation in the Central region of Brazil and the state of Paraná. The cycle is semi-early, the yield potential 2677 kg ha-1 and BRS Ártico has moderate resistance to rust and curtobacterium wilt.

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The ability of Phakopsora pachyrhizi to cause infection under conditions of discontinuous wetness was investigated. In in vitro experiments, droplets of a uredospore suspension were deposited onto the surface of polystyrene. After an initial wetting period of either 1, 2 or 4 h, the drops were dried for different time intervals and then the wetness was restored for 11, 10 or 8 h. Germination and appressorium formation were evaluated. In in vivo experiments, soybean plants were inoculated with a uredospore suspension. Leaf wetness was interrupted for 1, 3 or 6 h after initial wetting periods of 1, 2 or 4 h. Then, the wetting was re-established for 11, 10 or 8 h, respectively. Rust severity was evaluated 14 days after inoculation. The germination of the spores and the formation of the appressoria on the soybean leaves after different periods of wetness were also quantified in vivo by scanning electron microscopy. P. pachyrhizi showed a high infective capacity during short periods of time. An interruption of wetness after 1 h caused average reductions in germination from 56 to 75% and in appressorium formation from 84 to 96%. Rust severity was lower in all of the in vivo treatments with discontinuous wetness when compared to the control plants. Rust severity was zero when the interruption of wetness occurred 4 h after the initial wetting. Wetting interruptions after 1 and 2 h reduced the average rust severity by 83 and 77%, respectively. The germination of the uredospores on the soybean leaves occurred after 2 h of wetness, with a maximum germination appearing after 4 h of wetness. Wetness interruption affected mainly the spores that had initiated the germination.