Estudo comparativo do genoma e do transcriptoma de Phyllosticta citricarpa (fase sexual Guignardia citricarpa) agente da Mancha Preta dos Citros e Phyllosticta capitalensis

Pós-graduação em Ciências Biológicas (Genética) - IBB

Cryotolerance and global gene-expression patterns of Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Anotação de 500 ESTs da saúva Atta laevigata

Leaf-cutting ants belonging to the genus Atta occur from the tropical to subtropical regions of the Americas. These insects are considered pests because they cause serious damage in agricultural areas. Among these, stands out Atta laevigata, species which the colony requires a huge amount of leaves to grow its symbiotic fungus which is the main food source of the nest. Thus, the study of the transcriptome of these ants becomes a useful tool, because it is possible to identify proteins potentially involved with their skills as insect pests and also those related to differences between the varieties present in the nests. In the present study we described results of the partial analysis of the transcriptome of the leaf-cutting ant pest A. laevigata, from cDNA sequences previously generated in the Laboratory of Evolution and Molecular (LEM). The results may also be used for molecular, ecological, metabolic and evolutionary studies about ants, and heterologous expression of important proteins as molecular targets for the control of some leafcutting agricultural pests.

Aspectos clínicos, histopatológicos e expressão gênica do endométrio de cadelas acometidas por hiperplasia endometrial cística, mucometra e piometra

Pós-graduação em Cirurgia Veterinária - FCAV

Uso de microarray para determinação da expressão gênica diferencial em oócitos Bos Taurus Indicus e Bos Taurus Taurus submetidos ao estresse térmico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comprehensive genome methylation and whole genome expression analysis in penile carcinoma: Uncovering new molecular markers

Background: Penile carcinoma (PeCa) is frequently associated with high morbidity rates. Unlikely of the vast majority of tumors, there is no molecular markers described that are able to assist in diagnosis and prognosis or with potential to be therapeutic targets in PeCa. Patients and methods: DNA methylation status (244K Human DNA Methylation Microarray platform, Agilent Technologies) and large-scale expression analysis (4x44K Whole Human Genome Microarray, Agilent Technologies) were performed in 35 and 37 PeCa, respectively. Quantitative bisulfite pyrosequencing (qBP) and RT-qPCR were used to validate the findings in 93 samples. HPV status was assessed using the Linear Array HPV Genotyping kit (Roche Molecular Diagnostics, CA, USA). Results: Methylome analysis revealed 171 hypermethylated and 449 hypomethylated CpGs sites and the transcriptome profiling showed 2986 down- and 2817 over-expressed genes. HPV positivity was found in 32.7% of the cases, mainly the HPV16. The integrative analysis in 32 PeCa revealed a panel of 96 genes with inverse correlation between methylation and gene expression levels. The CpG hypermetlylation and gene downexpression, was confirmed for TWIST1, RSOP2, SOX3, SOX17, CD133, OTX2, HOXA3 and MEIS. In addition, BIRC5, DNMT1 and DNMT3B presented low levels of methylation and overexpression. The comparison of the results with clinical findings revealed that LIN28A, NKX2.2, NKX2.3, LHX5, BDNF, FOXA1 and CDX2 were associated with poor prognosis features. Conclusion: Putative prognostic markers were detected revealing that DNA methylation modulates the expression of several genes in PeCa. These data may prove instrumental for biomarker discovery in clinics and molecular epidemiology of PeCa.

Venomics of the Australian eastern brown snake (Pseudonaja textilis): detection of new venom proteins and splicing variants

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transcriptionally active LTR retrotransposons in Eucalyptus genus are differentially expressed and insertionally polymorphic

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Overexpression of mitochondrial uncoupling protein 1 (UCP1) induces a hypoxic response in Nicotiana tabacum leaves

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Retenção de clorofila em sementes de soja (Glycine max (L.) Merr.): estudos fisiológicos, bioquímicos e moleculares

Pós-graduação em Agronomia (Agricultura) - FCA

Análise do transcriptoma de folhas de cana-de-açúcar submetidas à prolongada limitação hídrica usando RNA-Seq

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise integrada entre dados de expressão global de transcritos codificadores e de miRNAs em carcinomas de células escamosas de laringe

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização transcriptômica de vias metabólicas na formiga Attini Atta sexdens rubropilosa Forel, 1908 (Hymenoptera, Formicidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão gênica diferencial em tecido muscular de bovinos Nelore divergentes para qualidade de carne

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise de parentesco e variabilidade genética de pacu (Piaractus mesopotamicus) por meio de marcadores SNPs: subsídios para o melhoramento genético

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)