Impacts of seawater acidification on mantle gene expression patterns of the Baltic Sea blue mussel: implications for shell formation and energy metabolism, link to supplementary material

Marine organisms have to cope with increasing CO2 partial pressures and decreasing pH in the oceans. We elucidated the impacts of an 8-week acclimation period to four seawater pCO2 treatments (39, 113, 243 and 405 Pa/385, 1,120, 2,400 and 4,000 µatm) on mantle gene expression patterns in the blue mussel Mytilus edulis from the Baltic Sea. Based on the M. edulis mantle tissue transcriptome, the expression of several genes involved in metabolism, calcification and stress responses was assessed in the outer (marginal and pallial zone) and the inner mantle tissues (central zone) using quantitative real-time PCR. The expression of genes involved in energy and protein metabolism (F-ATPase, hexokinase and elongation factor alpha) was strongly affected by acclimation to moderately elevated CO2 partial pressures. Expression of a chitinase, potentially important for the calcification process, was strongly depressed (maximum ninefold), correlating with a linear decrease in shell growth observed in the experimental animals. Interestingly, shell matrix protein candidate genes were less affected by CO2 in both tissues. A compensatory process toward enhanced shell protection is indicated by a massive increase in the expression of tyrosinase, a gene involved in periostracum formation (maximum 220-fold). Using correlation matrices and a force-directed layout network graph, we were able to uncover possible underlying regulatory networks and the connections between different pathways, thereby providing a molecular basis of observed changes in animal physiology in response to ocean acidification.

Seawater carbonate chemistry and processes during experiments with marine mussel, Mytilus galloprovincialis, 2005

In the context of future scenarios of progressive accumulation of anthropogenic CO2 in marine surface waters, the present study addresses the effects of long-term hypercapnia on a Mediterranean bivalve, Mytilus galloprovincialis. Sea-water pH was lowered to a value of 7.3 by equilibration with elevated CO2 levels. This is close to the maximum pH drop expected in marine surface waters during atmosextracellular pHric CO2 accumulation. Intra- and extracellular acid-base parameters as well as changes in metabolic rate and growth were studied under both normocapnia and hypercapnia. Long-term hypercapnia caused a permanent reduction in haemolymph pH. To limit the degree of acidosis, mussels increased haemolymph bicarbonate levels, which are derived mainly from the dissolution of shell CaCO3. Intracellular pH in various tissues was at least partly compensated; no deviation from control values occurred during long-term measurements in whole soft-body tissues. The rate of oxygen consumption fell significantly, indicating a lower metabolic rate. In line with previous reports, a close correlation became evident between the reduction in extracellular pH and the reduction in metabolic rate of mussels during hypercapnia. Analysis of frequency histograms of growth rate revealed that hypercapnia caused a slowing of growth, possibly related to the reduction in metabolic rate and the dissolution of shell CaCO3 as a result of extracellular acidosis. In addition, increased nitrogen excretion by hypercapnic mussels indicates the net degradation of protein, thereby contributing to growth reduction. The results obtained in the present study strongly indicate that a reduction in sea-water pH to 7.3 may be fatal for the mussels. They also confirm previous observations that a reduction in sea-water pH below 7.5 is harmful for shelled molluscs.

The State of the Nation Debate as part of the democratic parliamentary ritual: Rajoy and Rubalcaba discourse analysis, 2014

In this article we analyze the Debate on the State of the Nation 2014. The methodology consists in coding the speeches of the prime minister, Mariano Rajoy (PP) and the then opposition leader Alfredo Perez Rubalcaba (PSOE) through extracting word clouds, branched maps and word trees that have shown the most common concepts and premises. This preliminary analysis of two dimensions, quantitative and qualitative, makes it much easier and viable subsequent discourse analysis where we focus on the different types of arguments in the communicative act: claim/solution, circumstantial premises, goal premises, value premises, meansgoal premises, alternative options/addressing alternative options.

The influence of mussel-modified habitat on Fucus serratus L. a rocky intertidal canopy-forming macroalga

The influence of habitat modification by Mytilus edulis L. on the settlement and development of Fucus serratus populations was investigated on rocky shores of the Isle of Anglesey, North Wales. Settlement of fucoids was higher inside mussel habitat than outside on one of two shores studied. The effect of microhabitat on survival of fucoid germlings was examined by transplanting the germlings into and outside mussel habitats, each with and without the exclusion of grazers. Observation showed that periwinkles and top shells were abundant in mussel habitat, while limpets dominated bare rock. Exclusion of grazers greatly enhanced the survival of fucoid germlings in both habitats, indicating that while mussel habitat supports a different grazer assemblage to bare rock, both assemblages are important in limiting fucoid recruitment. The risk of dislodgement was assessed and compared between fucoids growing on mussel shells and bare rock. In situ pull-tests showed that less force was required to detach large fertile thalli growing on mussel shells than those growing on the rock. Adhesion was generally broken between the mussel and the rock rather than between the holdfast and the mussel. These observations indicate that mussels provide an unstable substrate for mature fucoids. Overall results suggest that a negative effect of mussel-modified habitat on fucoids is profound in adults; but the effect is context-dependent in juveniles and can be positive at settlement. Results from a survey on population structure of fucoids across two shores showed that there were greater numbers of large fertile fucoids growing directly attached to rock than on mussel shells, while there was no difference for juvenile fucoids confirming the experimental results. Moreover thalli larger than 60 cm were found only on the rock but not on shells. This finding suggests that a mussel dominated habitat may have a significant impact on reproductive output in fucoid populations.

The influence of mussel-modified habitat on Fucus serratus L. a rocky intertidal canopy-forming macroalga

The influence of habitat modification by Mytilus edulis L. on the settlement and development of Fucus serratus populations was investigated on rocky shores of the Isle of Anglesey, North Wales. Settlement of fucoids was higher inside mussel habitat than outside on one of two shores studied. The effect of microhabitat on survival of fucoid germlings was examined by transplanting the germlings into and outside mussel habitats, each with and without the exclusion of grazers. Observation showed that periwinkles and top shells were abundant in mussel habitat, while limpets dominated bare rock. Exclusion of grazers greatly enhanced the survival of fucoid germlings in both habitats, indicating that while mussel habitat supports a different grazer assemblage to bare rock, both assemblages are important in limiting fucoid recruitment. The risk of dislodgement was assessed and compared between fucoids growing on mussel shells and bare rock. In situ pull-tests showed that less force was required to detach large fertile thalli growing on mussel shells than those growing on the rock. Adhesion was generally broken between the mussel and the rock rather than between the holdfast and the mussel. These observations indicate that mussels provide an unstable substrate for mature fucoids. Overall results suggest that a negative effect of mussel-modified habitat on fucoids is profound in adults; but the effect is context-dependent in juveniles and can be positive at settlement. Results from a survey on population structure of fucoids across two shores showed that there were greater numbers of large fertile fucoids growing directly attached to rock than on mussel shells, while there was no difference for juvenile fucoids confirming the experimental results. Moreover thalli larger than 60 cm were found only on the rock but not on shells. This finding suggests that a mussel dominated habitat may have a significant impact on reproductive output in fucoid populations.

D9.5 – Exploitation Plan (version 1 of 3)

The RAGE Exploitation Plan is a living document, to be upgraded along the project lifecycle, supporting RAGE partners in defining how the results of the RAGE RIA will be used both in commercial and non-comercial settings. The Exploitation Plan covers the entire process from the definition of the business case for the RAGE Ecosystem to the creation of the sustainability conditions for its real-world operation beyond the H2020 project co-funding period. The Exploitation Plan will be published in three incremental versions, due at months 18, 36 and 42 of the project lifetime. This early stage version 1 of 3 is mainly devoted to: i. Setting-up the structure and the initial building blocks to be populated and completed in the future editions of the Exploitation Plan and to ii. providing additional guidance for market intelligence gathering, business modelling definition and validation, outreach and industry engagement and ultimately providing insights for the development, validation and evaluation of RAGE results across the project´s workplan execution. These tasks will in turn render suitable inputs to enhance the two future editions of the Exploitation Plan.

Viver com úlcera crónica nos membros inferiores: análise qualitativa de narrações significativas de pessoas idosas

O envelhecimento populacional é uma realidade mundial que altera a sociedade de forma complexa, implicando a necessidade de criação de estratégias de adaptação a esta realidade. A população idosa portuguesa apresenta também um aumento exponencial, e este fenómeno está frequentemente associado à perda de capacidades e dependência e ao aumento da incidência de patologias crónicas, como por exemplo as úlceras crónicas nos membros inferiores. Esta realidade verifica-se através de uma maior procura de cuidados de saúde e também no aumento dos encargos para a família e segurança social. Assim, a aquisição de conhecimentos sobre as implicações da úlcera crónica nos membros inferiores permite, aos profissionais de saúde, melhorar a prestação de cuidados às pessoas idosas, possibilitando a gestão eficaz de recursos e a melhoria da qualidade de vida dos utentes. Neste contexto, emergiu o nosso estudo que tem como objetivo conhecer as principais implicações da presença de uma úlcera crónica, nos membros inferiores, no quotidiano de pessoas idosas. A metodologia utilizada foi qualitativa, com um tipo de estudo exploratório-descritivo, em que foram realizadas 16 entrevistas a pessoas idosas portadoras de úlcera crónica nos membros inferiores e posteriormente analisadas as narrações de vivências ou experiências significativas dos participantes, utilizando as etapas metodológicas da análise de conteúdo segundo Bardin (2011). Os resultados encontrados foram incluídos em três áreas temáticas: Sentimentos e preocupações vividos com o aparecimento e desenvolvimento da úlcera crónica, Alterações no quotidiano das pessoas idosas com úlcera crónica e a Rede de apoio da pessoa idosa com úlcera crónica. A primeira área temática demostrou que as pessoas idosas apresentam sentimentos negativos de tristeza e dor em relação às suas vivências com a úlcera crónica, e receios futuros relacionados com a incerteza da evolução da úlcera, verificando-se alguma ambivalência entre a esperança e o desespero. As alterações no quotidiano verificaram-se na mobilidade física prejudicada, na interferência em atividades de vida diária e através da necessidade de tratamento. Na mobilidade física prejudicada foi o caminhar o mais mencionado pelos participantes e na interferência em atividades de vida diária foram as atividades domésticas, sociais e de lazer. A terceira área temática incluiu a rede de apoio da pessoa idosa com úlcera crónica identificando a família, a instituição e o convivente significativo como o principal apoio dos participantes. A família apresentou um papel de destaque, através do apoio prestado pelo cônjuge e pelos filhos. A realização deste estudo proporcionou conhecer melhor a realidade das pessoas idosas com úlceras crónicas nos membros inferiores, os seus sentimentos, dificuldades e/ou incapacidades, permitindo aos profissionais de saúde aumentar os conhecimentos e elaborar estratégias para auxiliar no seu dia-a-dia, ambicionando-se uma melhoria na prestação de cuidados às pessoas idosas, famílias e sociedade.

Long-term outcomes following “presumed” total parathyroidectomy for secondary hyperparathyroidism of chronic kidney disease

Aim. The most efficacious surgical treatment for renal hyperparathyroidism is still subject of research. Considering its low incidence rate of long-term relapse, “presumed” total parathyroidectomy without autotrasplantation (TP) may be indicated for secondary hyperparathyroidism (2HPT) in patients with chronic kidney disease (CKD), not eligible for kidney transplantation. The aim of this study was to analyse the TP long-term results in 2HPT haemodialysis (HD) patients. Method. Between January 2004 and October 2009, 25 2HPT HD patients, not eligible for kidney transplantation, underwent TP of at least four parathyroid glands. Clinical status and intact parathyroid hormone (iPTH) serum levels were assessed intraoperatively and during a 36-month follow-up. Results. TP improved the typical clinical symptoms and a significant reduction of iPTH serum levels was achieved in each patient. Aparathyroidism was never observed; in case of severe postoperative hypocalcemia, hypocalcemic seizures were never reported and the long-term recurrence rate was 8%. Only one patient received a kidney transplantation. Postoperative cardiovascular events (hypertension, peripheral artery disease, arrhythmia, coronary or cerebrovascular disease) were observed in 32% of cases and mortality rate was 16%. Conclusions. Considering its low long-term relapse rate and the absence of postoperative aparathyroidism, TP may still be considered the treatment of choice in patients with aggressive forms of 2HPT or of advanced dialytic vintage, with no access to renal transplantation. In case of postoperative hypoparathyroidism, hypocalcaemia can be effectively managed by medical treatment.

Sub lethal mercuric chloride toxicity induced stress related alterations in the epithelial lining of foot of the freshwater mussel Lamellidens marginalis (Lamarck)

Sub lethal (0.2 ppm) mercuric chloride induced stress related histopathological alterations in the epithelial linings of foot (podium) of the edible freshwater mussel Lamellidens marginalis (Lamarck) were studied using histochemical techniques up to 60 days of exposure. The histomorphological changes were manifested only slowly and its intensity was somewhat proportional to the duration of exposure. The immediate response of the exposed mussels was the altered mucous secretion. There was a progressive incorporation of sulphated glycoproteins into the secretory contents of the mucous cells especially in the first half of the experiment. Marked histopathological changes including necrosis, appearance of pyknotic nuclei, sloughing of epithelial cells and appearance of non-tissue spaces, etc., started appearing during the later half of the experiment. The fag end of the experiment, which witnessed prominent histomorphological changes, was accompanied by highly decreased mucous secretion. KEYWORDS: heavy metal toxicity, mercuric chloride, Lamellidens marginalis, freshwater mussel, histopathology.