Estudo da atividade ansiolítica e sedativa do óleo essencial das cascas de frutos de espécies do gênero Citrus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos silibina sobre modelo pré-eclâmpsia induzida em ratas por tratamento com Nω-Nitro-L-arginina metil ester

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Proteção renal com eritropoetina em modelo de isquemia renal no rato

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ação de biorreguladores na brotação, produção e algumas características físico-químicas de uva do cultivar Superior Seedless

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da toxicidade oral de 90 dias(doses repetidas)do lodo de estação de tratamento de esgoto(LETE) em ratos wistar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cryptomoschatone D2 from Cryptocarya mandioccana: cytotoxicity against human cervical carcinoma cell lines

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genotoxic assessment of Rubus imperialis (Rosaceae) extract in vivo and its potential chemoprevention against cyclophosphamide-induced DNA damage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Giberelina, citocinina e auxina na qualidade química de bagas de uva superior seedless

Trials were carried out in Juazeiro, Bahia State, Brazil, aiming to test plant regulators composed by gibberellin, cytokine and auxin effects on chemical quality of Superior Seedless grape berries. The first trial studied the effects of Stimulate (R) (bio regulator) and X-Cyte (R) (cytokine) associated to a new gibberellin formulation (N-Large (R)) and associated to Pro-Gibb (R), which is a product used as source of gibberellin. Products were sprayed at berries development phase (18, 21, 51 and 56 days after spur-pruning). Treatments were: T1: Pro-Gibb (R); T2: Stimulate (R) (Dose 1); T3: Stimulate (R) (Dose 2); T4: Stimulate (R) (Dose 3); T5: Pro-Gibb (R) + X-Cyte (R) (Low Dose - DB); T6: Pro-Gibb (R) + X-Cyte (R) (Intermediate Dose - DM); T7: Pro-Gibb (R) + X-Cyte (R) (High Dose - DA); T8: N-Large (R); T9: N-Large (R) + X-Cyte (R) (DB); T10: N-Large (R) + X-Cyte (R) (DM); T11: N-Large (R) + X-Cyte (R) (DA). The second trial aimed to assess the effect of the new gibberellin formulation (N-Large (R)) associated or not with cytokine (X-Cyte (R)) also sprayed straight over the bunches at berries development phase (17, 55 e 66 days after spur-pruning). Treatments were: T1: Pro-Gibb (R) - blank; T2: N-Large (R) (DB); T3: N-Large (R) (DM); T4: N-Large (R) (DA); T5: N-Large (R) (DB) + X-Cyte (R) (DB); T6: N-Large (R) (DB) + X-Cyte (R) (DM); T7: N-Large (R) (DB) + X-Cyte (R) (DA); T8: N-Large (R) (DM) + X-Cyte (R) (DB); T9: N-Large (R) (DM) + X-Cyte (R) (DM); T10: N-Large (R) (DM) + X-Cyte (R) (DA); T11: N-Large (R) (DA) + X-Cyte (R) (DB); T12: N-Large (R) (DA) + X-Cyte (R) (DM); T13: N-Large (R) (DA) + X-Cyte (R) (DA). Experimental design was random blocks with four repetitions with each repetition/parcel having three useful plants in the same row. At harvest, when bunches average had soluble solids over 15 degrees Brix, berries were collected for soluble solids, pH, titratable acidity analysis as well as (SS/AT) ratio calculation. In both trials, plant regulators evaluated did not provide significant changes on chemical quality of 'Superior Seedless' grape berries. Therefore, the lack of differences on response between the commercially used product (Pro-Gibb (R)) and the other products tested (Stimulate (R), X-Cyte (R) e N-Large (R)) prove the last as promising for the ` Superior Seedless' grape cultivation, leaving a larger range of alternative for grape farmers in the Sao Francisco Valley, Bahia.

Métodos diagnóstico de Hiperadrenocorticismo canina

The Hyperadrenocorticism is a disease that occur in dogs between mead to old age, is associated with the higher level of glucocorticoid that result in a corporal reaction. To make a diagnostic of this disease is necessary have a good history and physical examination, the principals complains of the proprietary are polyuria, polydipsia and polyphagia, in some times abdominal enlargement and lethargy. In the physical examination is observed this abdominal enlargement, hepatomegaly, alopecia, in some cases pyoderma and difficult respiratory. From this documents is possible determinate the differential diagnostics and with the tests find the diagnostic of the disease that the animal have. The base methods are hemogram, biochemical, urinalysis, radiographs and ultrasonography it’s possible do tomography and magnetic resonance too. There are confirmation methods too, they are very important, because the others not have documents necessary for the final diagnostic. Those are Urine cortisol to creatinine ratio, dexamethasone suppression test with high dose or low dose and ACTH stimulation test. Despite those tests have high sensibility and specificity, they can result in false positive or false negative, this is one of the causes to do the base methods and together make the veterinary doctor find the solution of the diagnostic and do the adequate treatment

Gene expression of estrogen receptor-alpha in orbital fibroblasts in Graves’ ophthalmopathy

Graves’ ophthalmopathy (GO) is one of the most severe clinical manifestations of Graves’ disease (GD), and its treatment might involve high-dose glucocorticoid therapy. The higher incidence of GO among females, and the reported association between polymorphisms of estrogen receptor (ER) and GD susceptibility have led us to question the role of estrogen and its receptor in GO pathogenesis. We, thus, assessed estrogen receptor-alpha (ERA) gene expression in cultures of orbital fibroblasts from a patient with GO before (controls) and after treatment with 10 nM and 100 nM dexamethasone (DEX). Orbital fibroblasts showed ERA gene expression. In the cells treated with 10 nM and 100 nM DEX, ERA gene expression was, respectively, 85% higher and 74% lower, than in the control group. We concluded that ERA gene expression is found in the orbital fibroblasts of patient with GO, which may be affected by glucocorticoids in a dose-related manner. Arch Endocrinol Metab. 2015;59(3):273-6

Ethanol modulates the synthesis and catabolism of retinoic acid in the rat prostate

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of the gadolinium ion on body fluid regulation

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação da cultura do milho submetida à hormesis

Under conservation tillage systems is critical to maintaining plant residues from previous crops on the soil surface. A technique called Hormesis aims to increase the amount of straw and also cause a delay in straw decomposition. Therefore, this study aimed to evaluate the agronomic characteristics of corn under no-tillage system subjected to Hormesis. The experiment was carried out at UNESP campus in the city of Botucatu, SP . The experimental was design in completely randomized blocks. The treatments based on the technique of Hormesis were : control (no application) , sub Gliphosate low dosage (12.5 g.ai.ha - 1 ) , sub Gliphosate average dosage (25 g. ai.ha - 1 ) , sub Gliphosate high dose (50 g. ai.ha -1 ), 2,4-D under low dose (100 g. ai.ha -1 ), 2,4-D sub average dosage (200 g. ai.ha -1 ) 2,4- D under high dosage (300 g. ai.ha -1 ) sub Verdict low dose (0.625 g. ai.ha -1 ) sub Verdict average dosage (1.25 g. ai.ha -1 ) , sub Verdict high dosage (2.5 g. ai.ha - 1 ) . In addition, the following characteristics were evaluated: plant height, first ear growth, stem diameter , ear length , number of rows per cob, cob diameter , , percentage of grains on the cob , mass of plant dry matter, and yield. The results showed that all the treatments showed no statistical difference, the maize was not affected with sub doses of herbicides applications under the field and weather conditions in which the experiment was conducted.

Development of a Model of Natural Infection with Mycobacterium bovis in White-Tailed Deer

The objective of this study was to develop a suitable experimental model of natural Mycobacterium bovis infection in white-tailed deer (Odocoileus virginianus), describe the distribution and character of tuberculous lesions, and to examine possible routes of disease transmission. In October 1997, 10 mature female white-tailed deer were inoculated by intratonsilar instillation of 2 3 103 (low dose) or 2 3 105 (high dose) colony forming units (CFU) of M. bovis. In January 1998, deer were euthanatized, examined, and tissues were collected 84 to 87 days post inoculation. Possible routes of disease transmission were evaluated by culture of nasal, oral, tonsilar, and rectal swabs at various times during the study. Gross and microscopic lesions consistent with tuberculosis were most commonly seen in medial retropharyngeal lymph nodes and lung in both dosage groups. Other tissues containing tuberculous lesions included tonsil, trachea, liver, and kidney as well as lateral retropharyngeal, mandibular, parotid, tracheobronchial, mediastinal, hepatic, mesenteric, superficial cervical, and iliac lymph nodes. Mycobacterium bovis was isolated from tonsilar swabs from 8 of 9 deer from both dosage groups at least once 14 to 87 days after inoculation. Mycobacterium bovis was isolated from oral swabs 63 and 80 days after inoculation from one of three deer in the low dose group and none of four deer in the high dose group. Similarly, M. bovis was isolated from nasal swabs 80 and 85 days after inoculation in one of three deer from the low dose group and 63 and 80 days after inoculation from two of four deer in the high dose group. Intratonsilar inoculation with M. bovis results in lesions similar to those seen in naturally infected white-tailed deer; therefore, it represents a suitable model of natural infection. These results also indicate that M. bovis persists in tonsilar crypts for prolonged periods and can be shed in saliva and nasal secretions. These infected fluids represent a likely route of disease transmission to other animals or humans.

Investigation of the Transmission of Mycobacterium bovis from Deer to Cattle Through Indirect Contact

Objective—To investigate the infection of calves with Mycobacterium bovis through oral exposure and transmission of M bovis from experimentally infected white-tailed deer to uninfected cattle through indirect contact. Animals—24 11-month-old, white-tailed deer and 28 6-month-old, crossbred calves. Procedure—In the oral exposure experiment, doses of 4.3 X 106 CFUs (high dose) or 5 X 103 CFUs (low dose) of M bovis were each administered orally to 4 calves; as positive controls, 2 calves received M bovis (1.7 X 105 CFUs) via tonsillar instillation. Calves were euthanatized and examined 133 days after exposure. Deer-to-cattle transmission was assessed in 2 phases (involving 9 uninfected calves and 12 deer each); deer were inoculated with 4 X 105 CFUs (phase I) or 7 X 105 CFUs (phase II) of M Bovis. Calves and deer exchanged pens (phase I; 90 days’ duration) or calves received uneaten feed from deer pens (phase II; 140 days’ duration) daily. At completion, animals were euthanatized and tissues were collected for bacteriologic culture and histologic examination. Results—In the low- and high-dose groups, 3 of 4 calves and 1 of 4 calves developed tuberculosis, respectively. In phases I and II, 9 of 9 calves and 4 of 9 calves developed tuberculosis, respectively. Conclusions and Clinical Relevance—Results indicated that experimentally infected deer can transmit M bovis to cattle through sharing of feed. In areas where tuberculosis is endemic in free-ranging white-tailed deer, management practices to prevent access of wildlife to feed intended for livestock should be implemented.