Biological, biochemical and molecular features of Trypanosoma cruzi strains isolated from patients infected through oral transmission during a 2005 outbreak in the state of Santa Catarina, Brazil: its correspondence with the new T. cruzi Taxonomy Consensus (2009)

We examined strains of Trypanosoma cruzi isolated from patients with acute Chagas disease that had been acquired by oral transmission in the state of Santa Catarina, Brazil (2005) and two isolates that had been obtained from a marsupial (Didelphis aurita) and a vector (Triatoma tibiamaculata). These strains were characterised through their biological behaviour and isoenzymic profiles and genotyped according to the new Taxonomy Consensus (2009) based on the discrete typing unities, that is, T. cruzi genotypes I-VI. All strains exhibited the biological behaviour of biodeme type II. In six isolates, late peaks of parasitaemia, beyond the 20th day, suggested a double infection with biodemes II + III. Isoenzymes revealed Z2 or mixed Z1 and Z2 profiles. Genotyping was performed using three polymorphic genes (cytochrome oxidase II, spliced leader intergenic region and 24Sα rRNA) and the restriction fragment length polymorphism of the kDNA minicircles. Based on these markers, all but four isolates were characterised as T. cruzi II genotypes. Four mixed populations were identified: SC90, SC93 and SC97 (T. cruzi I + T. cruzi II) and SC95 (T. cruzi I + T. cruzi VI). Comparison of the results obtained by different methods was essential for the correct identification of the mixed populations and major lineages involved indicating that characterisation by different methods can provide new insights into the relationship between phenotypic and genotypic aspects of parasite behaviour.

HIV-1 tropism and CD4 T lymphocyte recovery in a prospective cohort of patients initiating HAART in Ribeirão Preto, Brazil

While human immunodeficiency virus (HIV)-1 chemokine co-receptors 5 tropism and the GWGR motif in the envelope third variable region (V3 loop) have been associated with a slower disease progression, their influence on antiretroviral response remains unclear. The impact of baseline V3 characteristics on treatment response was evaluated in a randomised, double blind, prospective cohort study with patients initiating highly active antiretroviral therapy with lopinavir or efavirenz plus azithothymidine/3TC (1:1) over 48 weeks. Similar virological and immunological responses were observed for both treatment regimens. The 43 individuals had a mean baseline CD4 T cell count of 119 cells/mm³ [standard deviation (SD) = 99] and a mean viral load of 5.09 log10 copies/mL (SD = 0.49). The GWGR motif was not associated with a CD4 T cell response, but predicted R5 tropism by the geno2pheno[clinical20%] algorithm correlated with higher CD4 T cell levels at all monitoring points (p < 0.05). Moreover, higher false-positive rates (FPR) values from this analysis revealed a strong correlation with CD4 T cell recovery (p < 0.0001). Transmitted drug resistance mutations, documented in 3/41 (7.3%) cases, were unrelated to the assigned antiretroviral regimen and had no impact on patient outcomes. In conclusion, naÏve HIV-1 R5 infected patients exhibited higher CD4 T cell counts at baseline; this difference was sustained throughout therapy. The geno2pheno[clinical] option FPR positively correlated with CD4 T cell gain and may be useful in predicting CD4 T cell recovery.

Antiviral therapy against chronic hepatitis B in Brazil: high rates of lamivudine resistance mutations and correlation with HBV genotypes

The effectiveness of antiviral treatments of chronic hepatitis B has been poorly studied in Brazil. Here, hepatitis B virus (HBV) DNA positivity, drug resistance mutations and their association with HBV genotypes were evaluated in chronically HBV-infected patients under different drug regimens in Brazil. The study involved 129 patients under interferon or nucleos(t)ide analogue therapy for a median treatment time of 12 months. One hundred and five (81%) of these patients were treated with lamivudine (LAM), either in monotherapy or in combination with newer drugs, such as entecavir (ETV) or tenofovir (TDF). High (37.5-100%) rates of HBV DNA positivity were observed with all but one drug regimen (LAM + ETV). However, patients that were treated with ETV alone, TDF alone or with LAM combination therapies had a mean viral load that was 3-4 log lower than patients treated with LAM monotherapy. Of the patients treated with LAM, 47% developed resistance mutations. HBV genotypes A (59.1%), D (30.3%) and F (9.1%) were found. There was no association between the presence of LAM resistance mutations and genotypes, HBeAg status or treatment duration. Nevertheless, the rtM204V mutation was observed more frequently (12/13, 92%) in genotype A than in the others (p = 0.023). Six out of nine isolates that contained the rtM204I mutation belonged to genotype D and half of them displayed a single mutation. Genotype D isolates with the rtM204V variant preferentially displayed a triple mutation, while genotype A preferentially displayed a double mutation (p = 0.04).

Detection of the first incidence of Akodon paranaensis naturally infected with the Jabora virus strain (Hantavirus) in Brazil

We characterised hantaviruses circulating in different Akodon rodent species collected in midwestern Santa Catarina (SC), southern Brazil, where the Jabora hantavirus (JABV) strain was first identified in Akodon montensis. Genetic and phylogenetic analyses based on a partial S segment indicated that, in SC, Akodon paranaensis and A. montensis carried the same type of hantavirus. Additionally, we conducted the first genomic characterisation of the complete S segment from the Brazilian JABV strain. This is the first report of A. paranaensis infected with the JABV.

O Cabo-verdiano através dos olhos de forasteiros: representações nos textos portugueses, 1784-1844

Ao ler os textos portugueses dos finais do século XVIII e a primeira metade do século XIX deparase com uma certa depreciação e africanização do homem cabo-verdiano. As formas de sociabilidade dos cabo-verdianos eram reprovadas por estes serem demasiados próximo dos “negros africanos”. Estas representações continuam a ser menos conhecidas tanto no domínio da História como nos outros campos do saber. Ora, o presente trabalho debruça-se sobre a imagem do homem caboverdiano construída, pensada, e dada a ler nos textos portugueses produzidos pelos forasteiros no período entre 1784 e 1844. O corpo textual que sustenta este estudo foi produzido a partir do contacto com as ilhas e os seus habitantes ou, muitas vezes, a partir de informações de terceiros, por alguém cujos padrões mentais e culturais pertenciam à outra realidade. Da longa relação dos portugueses/europeus com os africanos sob a soberania portuguesa no espaço cabo-verdiano desenvolveu-se uma cultura nova e um homem novo – uma nova sociedade, que por um lado reflecte o fracasso português na assimilação dos cabo-verdianos e por outro mostra a capacidade de, num espaço novo, através do processo de mestiçagem, que foi quase um fenómeno natural nas ilhas de Cabo Verde, surgir algo novo, com contornos próprios, que se pode caracterizar de caboverdiano

Forms and accumulation of copper and zinc in a sandy typic hapludalf soil after long-term application of pig slurry and deep litter

Successive applications of pig slurry and pig deep litter may lead to an accumulation of copper (Cu) and zinc (Zn) fractions in the soil profile. The objective of this study was to evaluate the Cu and Zn forms and accumulation in a Sandy Typic Hapludalf soil after long-term application of pig slurry and deep litter. In March 2010, eight years after initiating an experiment in Braço do Norte, Santa Catarina (SC), Brazil, on a Sandy Typic Hapludalf soil, soil samples were collected from the 0-2.5, 2.5-5.0, 5-10 and 10-15 cm layers in treatments consisting of no manure application (control) and with applications of pig slurry and deep litter at two levels: the single and double rate of N requirement for maize and black oat succession. The soil was dried, ground in an agate mortar and analyzed for Cu and Zn contents by 0.01 mol L-1 EDTA and chemically fractionated to determine Cu and Zn. The applications of Pig deep litter and slurry at doses equivalent to 90 kg ha-1 N increased the contents of available Cu and Zn in the surface soil layer, if the double of this dose was applied in pig deep litter or double this dose in pig slurry, Cu and Zn migrated to a depth of 15 cm. Copper is accumulated mainly in the organic and residual fractions, and zinc preferentially in the fraction linked to clay minerals, especially in the surface soil layers.

Pedotransfer functions to estimate retention and availability of water in soils of the state of Santa Catarina, Brazil

Studies on water retention and availability are scarce for subtropical or humid temperate climate regions of the southern hemisphere. The aims of this study were to evaluate the relations of the soil physical, chemical, and mineralogical properties with water retention and availability for the generation and validation of continuous point pedotransfer functions (PTFs) for soils of the State of Santa Catarina (SC) in the South of Brazil. Horizons of 44 profiles were sampled in areas under different cover crops and regions of SC, to determine: field capacity (FC, 10 kPa), permanent wilting point (PWP, 1,500 kPa), available water content (AW, by difference), saturated hydraulic conductivity, bulk density, aggregate stability, particle size distribution (seven classes), organic matter content, and particle density. Chemical and mineralogical properties were obtained from the literature. Spearman's rank correlation analysis and path analysis were used in the statistical analyses. The point PTFs for estimation of FC, PWP and AW were generated for the soil surface and subsurface through multiple regression analysis, followed by robust regression analysis, using two sets of predictive variables. Soils with finer texture and/or greater organic matter content retain more moisture, and organic matter is the property that mainly controls the water availability to plants in soil surface horizons. Path analysis was useful in understanding the relationships between soil properties for FC, PWP and AW. The predictive power of the generated PTFs to estimate FC and PWP was good for all horizons, while AW was best estimated by more complex models with better prediction for the surface horizons of soils in Santa Catarina.

SOIL QUALITY IN RELATION TO FOREST CONVERSION TO PERENNIAL OR ANNUAL CROPPING IN SOUTHERN BRAZIL

Many forested areas have been converted to intensive agricultural use to satisfy food, fiber, and forage production for a growing world population. There is great interest in evaluating forest conversion to cultivated land because this conversion adversely affects several soil properties. We examined soil microbial, physical, and chemical properties in an Oxisol (Latossolo Vermelho distrófico) of southern Brazil 24 years after forest conversion to a perennial crop with coffee or annual grain crops (maize and soybeans) in conventional tillage or no-tillage. One goal was to determine which soil quality parameters seemed most sensitive to change. A second goal was to test the hypothesis that no-tillage optimized preservation of soil quality indicators in annual cropping systems on converted land. Land use significantly affected microbial biomass and its activity, C and N mineralization, and aggregate stability by depth. Cultivated sites had lower microbial biomass and mineralizable C and N than a forest used as control. The forest and no-tillage sites had higher microbial biomass and mineralizable C and N than the conventional tillage site, and the metabolic quotient was 65 and 43 % lower, respectively. Multivariate analysis of soil microbial properties showed a clear separation among treatments, displaying a gradient from conventional tillage to forest. Although the soil at the coffee site was less disturbed and had a high organic C content, the microbial activity was low, probably due to greater soil acidity and Al toxicity. Under annual cropping, microbial activity in no-tillage was double that of the conventional tillage management. The greater microbial activity in forest and no-tillage sites may be attributed, at least partially, to lower soil disturbance. Reducing soil disturbance is important for soil C sequestration and microbial activity, although control of soil pH and Al toxicity are also essential to maintain the soil microbial activity high.

El treball assalariat en els masos de la Catalunya interior al segle XIX. L'exemple del mas Santmartí

Malgrat que el mas es considera una unitat d'explotació de caracter familiar, les múltiples tasques que s'hi havien de fer obligaven sovint els amos a cercar mà d'obra fora de la familia. Aquesta necessitat augmentava a mesura que el conjunt de les terres sobrepassava I'extensió necessaria per a garantir I'autosuficiència familiar. En aquest article ens proposem d'analitzar el volum i la incidència del treball dels jornalers en el conjunt de les tasques d'un mas, a travès de l'exemple del mas Santmartí. Es tracta d'un mas que actualment pertany al municipi de Sallent, a la comarca del Bages, que ja apareix documentat a l'edat mitjana com un dels masos integrants de la quadra i la parroquia de Sant Martí de Serraïma. Va sobreviure a la crisi del segle XIV, de la qual es va beneficiar aglutinant masos rònecs. A partir del segle XVIII va iniciar una expansió del conreu dels cereals, de l'olivera i, sobretot, de la vinya, que es va mantenir fins a la segona meitat del XIX -cal no oblidar que el Bages era una comarca essencialment vitícola. Però, tot i que els amos van incrementar el nombre de vinyes que explotaven directament, van optar per establir-ne una gran part a rabassa. Així, doncs, segons l'amillarament, l'any 1861 l'hereu portava en conreu directe 23 Q 1 lq de cereals, 6 Q d'oliveres i 35 Q 4q de vinya. Alhora, 96 Q 4 q eren menades per rabassaires. És evident que les extensions de conreus explotades directament pels hereus exigien ma d'obra suplementària, de fora del nucli familiar.

Antroponímia i toponímia en un capbreu de Conesa de mitjan segle XVIII

Estudi d’un capbreu del terme de Conesa del segle xviii dipositat en el fons notarial de Valls, des del punt de vista de l’onomàstica, els terratinents i el noms de les partides de terra que s ́hi conreaven. El treball detalla la presència dels cognoms anotats des del segle XVII fins al XIX i si els noms dels topònims relacionats en aquest document són vius actualment.

Mating disruption with low density diffusers for the management of oriental fruit moths (Lepidoptera: Tortricidae) in apple orchards under subtropical climate in southern Brazil

The oriental fruit moth, Grapholita molesta Busck, and fruit flies, Anastrepha fraterculus L., are the important apple pests under Subtropical climate in Southern Brazil, and control is normally accomplished with insecticides. An alternative strategy for the control of G. molesta is mating disruption, through the use of pheromones. Mating disruption strategies using a low density of dispensers (20) per hectare were tested in comparison with conventional pesticides for control of G. molesta in commercial Gala apple orchards in Fraiburgo, SC, for a period of five years. The average field efficiency period of mating disruption formulation over five years was 113 days. In this period the mating interruption index on mating disruption plots was 84.8% over five years. Damage to Gala apples by oriental moth larvae was low (<0.1%) in mating disruption plots but did not differ from conventional plots, except in the third year. The use of mating disruption allowed for an average reduction of 5.2 insecticide treatments per year in Gala orchards during field efficiency period. It was necessary to apply 1.0 and 1.2 applications of insecticide to control of G. molesta and A. fraterculus, respectively. Mating disruption with a low density of diffusers proved to be an effective alternative to conventional methods for control of G. molesta in Gala apple orchards in subtropical climate in southern Brazil.

Nuevas series anuales de población de América Latina desde el siglo XIX hasta el 2000

El estudio de la población en América Latina es un tema central en la historiografía de la región, sin embargo, las distintas estimaciones existentes muestran importantes discrepancias para los siglos XIX y XX. A partir del contraste de distintas bases de datos, la principal contribución del artículo consiste en ofrecer nuevas series de población homogéneas para el conjunto de países de América Latina, junto a una detallada explicación de la obtención de los datos, así como un análisis de las discrepancias que las distintas fuentes muestran. Los países que aborda este trabajo son Argentina, Brasil, Bolivia, Chile, Colombia, Costa Rica, Cuba, Guatemala, Ecuador, Haití, Honduras, México, Nicaragua, Panamá, Paraguay, Perú, República Dominicana, Uruguay y Venezuela; a lo que se agrega la suma de todos ellos para obtener la población latinoamericana. Estas nuevas series pueden resultar de gran utilidad para reinterpretar la historia económica de América Latina en el largo plazo. The analysis of population levels in Latin America plays an important role in the regional historiography. The estimated series appeared until now offers huge discrepancies, therefore, we believe essential to provide homogeneous series for the 19th and the 20th centuries. In our work we shed new light on this issue, from an exhaustive study of the existing Latin American historical sources for the region. Along with a detailed explanation of the data collection, we also provide an analysis of the discrepancies and the accuracy of sources. The study offers data from 21 countries in Latin America: Argentina, Brazil, Bolivia, Chile, Colombia, Costa Rica, Cuba, Guatemala, Ecuador, Haiti, Honduras, Mexico, Nicaragua, Panama, Paraguay, Peru, Dominican Republic, Uruguay and Venezuela. This new evidence can be a crucial information to revisit Latin American Economic History in the long run.

A influência da Química nos saberes médicos acadêmicos e práticos do século XVIII em Portugal e no Brasil

At the dawn of the eighteenth century chemistry was establishing itself as a physical science on its own right, after a long ancillary relationship with medicine and pharmacy, which had began two centuries before. This association, and the many changes that came along the scientific revolution spread into many walks of life. The Luso-Brazilian world, apparently so removed from the new developments, could not help to be touched by them, as this study shows, in which two contemporary medical authors are analysed. Both were Portuguese who had long lived in Brazil; both practised and wrote extensively on Medicine; both felt the influence of the new times, albeit in quite different ways.

Aportació de les traduccions rosselloneses del segle XIX a la construcció del sistema lingüisticoliterari català

Qui vulgui parlar de les traduccions catalanes impreses al Rosselló ha de fer un apunt inevitable i esmentar els orígens de la impremta rossellonesa. El primer llibre imprès a Perpinyà, l’any 1502, és el Vocabulari molt profitos per aprendre lo Catalan Alamany y lo Alamany Catalan. És un extens glossari bilingüe per a la traducció de cent quaranta- dues pàgines. El segon llibre imprès a Perpinyà, l’any 1503, és una traducció del llatí feta per Narcís Solà. És un llibre renaixentista de medicina de Pere de Argilata: Aci comensa la cirurgia del reverende meritissim doctor en arts e en medicina Le reverent mestre Pere de Argilata de la ciutat de Bolonia d’una extensió de tres-centes vuit pàgines. Així doncs, la impremta al Rosselló començà amb dues traduccions. En el segle immediatament anterior al del nostre estudi, el segle xviii, podem comptar al Rosselló fins a vint-i-nou llibres impresos en català1 dels quals set són traduccions: quatre del francès, dues del castellà i una del llatí. Tant les set traduccions com els altres vint-idos llibres són de temàtica religiosa. Llibres de pietat, exercicis espirituals i un catecisme. Destaca, en el grup de les set traduccions, una tragèdia clàssica de tema bíblic Esther: tragedia santa de Jean Racine.

Negocios sentimentales. Familia, corporación y red mercantil en Argentina durante el siglo XIX

Nuestras unidades analíticas, están constituidas por dos familias dedicadas al comercio cuyo recorrido nos permite analizar un arco temporal amplio de prácticas y estrategias de racionalidad empresaria durante el siglo XIX argentino. El debate que guía el trabajo puede resumirse en la preponderancia de los lazos de parentesco o en el predominio de estrategias de tipo corporativo a la hora de articular la lógica mercantil. El desafío de nuestra propuesta es plantear el funcionamiento corporativo, las prácticas empresariales, y la integración político/institucional como variables fundamentales que compensaban la debilidad de los vínculos de parentesco. Los dos casos contrastados exudan similar funcionamiento en cuanto al origen inmigratorio de sus responsables y a las estrategias utilizadas para integrarse en ciudades portuarias de reciente importancia pero con una ambición común: convertirse en centros neurálgicos de la vida mercantil. El primer caso atañe a un comerciante catalán llegado en la segunda mitad del siglo XVIII, que construyó su patrimonio y su casa mercantil a través de un complejo entramado de redes sociales. El segundo ejemplo corresponde a un grupo empresario, llegado cien años después y dispuesto a condensar lazos identitarios con la ocupación de lugares claves de la vida mercantil e industrial regional. Ambos casos comparten la ausencia de una base familiar que garantizara la supervivencia patrimonial, una carencia que permite rediscutir el rol de la familia.