Complexity of the erythroid transcription factor NF-E2 as revealed by gene targeting of the mouse p18 NF-E2 locus.

High-level globin expression in erythroid precursor cells depends on the integrity of NF-E2 recognition sites, transcription factor AP-1-like protein-binding motifs, located in the upstream regulatory regions of the alpha- and beta-globin loci. The NF-E2 transcription factor, which recognizes these sites, is a heterodimer consisting of (i) p45 NF-E2 (the larger subunit), a hematopoietic-restricted basic leucine zipper protein, and (ii) a widely expressed basic leucine zipper factor, p18 NF-E2, the smaller subunit. p18 NF-E2 protein shares extensive homology with the maf protooncogene family. To determine an in vivo role for p18 NF-E2 protein we disrupted the p18 NF-E2-encoding gene by homologous recombination in murine embryonic stem cells and generated p18 NF-E2-/- mice. These mice are indistinguishable from littermates throughout all phases of development and remain healthy in adulthood. Despite the absence of expressed p18 NF-E2, DNA-binding activity with the properties of the NF-E2 heterodimer is present in fetal liver erythroid cells of p18 NF-E2-/- mice. We speculate that another member of the maf basic leucine zipper family substitutes for the p18 subunit in a complex with p45 NF-E2. Thus, p18 NF-E2 per se appears to be dispensable in vivo.

goDesign

This research project (September 2009 - ongoing) builds on initial research funded by a 2009 QUT Engagement Innovation Grant, which examined the benefits for rural and regional communities linking with tertiary institutions and design practitioners, to participate in design-based learning activities. During the program, students and teachers were given the opportunity to explore, analyse and re-imagine their local town through a series of scaffolded problem solving activities around the theme of ‘place’. Underpinning the program is Dr Charles Burnette’s (1993) IDESIGN teaching model and a place-based approach that ‘draws upon local cultural, environmental, economic and political concerns’ (Smith, G.A. 2007, p18).

The traditional fishery of Ibu project area on the northern portion of the Imo River, Nigeria

A survey carried out on the fishery of the northern portion of Imo basin (known as Ibu project area for the purpose of this study) provided some information on the number and type of fishermen cropping this portion of the Imo River, Nigeria. The population was found to be very mobile with many itinerant or seasonal fishermen. The type of gear in use was studied and a high number of traditional dug-out canoes recorded which shows the ability of the people to make their own canoes. The presence of freshwater shrimps crayfish and clupeids at this upper region of the Imo River was observed

Variabilidade genómica de Citrus tristeza virus e transformação genética de Citrus aurantium L. com vista à obtenção de resistência

Tese de dout., Biologia, Faculdade de Engenharia de Recursos Naturais, Univ. do Algarve, 2003

Développement de la sensibilité des neurones du collicule supérieur à la durée de la stimulation auditive

Chez plusieurs espèces, les neurones auditifs sensibles à la durée de la stimulation sont présents au niveau des collicules inférieurs. Toutefois, le décours temporel de leur développement fonctionnel est inconnu. Étant donné que le collicule supérieur est l’un des principaux relais sous-cortical impliqué dans l’intégration des stimuli audio-visuels, nous voulons déterminer si le collicule supérieur du rat contient de tels neurones et s’ils sont sensibles et sélectifs à différentes durées de stimulation auditive. De plus, l'originalité de cette étude est de déterminer les étapes de leur maturation fonctionnelle. Des enregistrements neuronaux unitaires et extra-cellulaires sont effectués dans le collicule supérieur de rats juvéniles (P15-P18, P21-P24, P27-P30) et adultes anesthésiés. La sensibilité à la durée est déterminée lors de la présentation de bruits gaussiens (2-10 dB SPL au-dessus du seuil) de durées variables (3-100 ms). Seulement un faible pourcentage des neurones du collicule supérieur est de type passe-bande (3-9% des neurones parmi les ratons et 20% chez les rats adultes). Une différence significative de la distribution entre les différents types de neurones auditifs sensibles à la durée est présente au cours du développement: les neurones de type passe-haut (63-75%) sont présents en majorité chez les groupes juvéniles alors que 43% des neurones sont de type insensible à la durée de la stimulation auditive chez les rats adultes. Ces résultats montrent qu’une population importante de neurones auditifs du collicule supérieur du rat est sensible à la durée des signaux sonores et qu’un développement fonctionnel important survient au cours du premier mois postnatal.

Observations on the nonlinear unloading behavior of advanced high strength steels

The unloading behavior was compared for three different steel grades: a dual-phase steel, a transformation-induced plasticity steel, and a twinning-induced plasticity steel. Steels that harden by phase transformation or deformation twinning exhibited a smaller component of microplastic strain during unloading and a smaller reduction in the chord modulus compared to the conventional hardening steel. As a result, unloading is closer to pure elastic unloading when the TRIP effect or TWIP effect is active.

Avaliação da correlação entre os parâmetros, carbono orgânico total e elementos tóxicos, em solo de um posto de serviço e revenda de combustíveis

Today a major responsibility for the contamination of soil and groundwater and surface water are establishments known as gas stations of fuel which has attracted increasing attention from both the general population as the state agencies of environmental control due to leaks in storage tanks and mainly to disruption of pipe corrosion of tanks and pumping. Other services, like oil changes and car wash are also causes for concern in this type of establishment. These leaks can cause or waste produced, and the contamination of aquifers, serious health problems and public safety, since most of these stations located in urban areas. Based on this, the work was to evaluate soil contamination of a particular service station and fuel sales in the city of Natal, through the quantification of heavy metals like Cd, Cu, Cr, Ni, Pb, Zn of total organic carbon (TOC) and organic matter using different techniques such as optical emission spectrometry with inductively coupled plasma source (ICP OES), Total Organic Carbon analyzer and gravimetric analysis respectively. And also to characterize the soil through particle size analysis. Samples were taken in 21 georeferenced points and collected in the same period. The soils sampled in sampling stations P3, P5, P6, P10, P11, P12, P13, P14, P15, P17, P18 and P20 showed the smallest size fractions ranging from fine sand to medium sand. The other study sites ranged from fine sand to medium sand, except the point P8 showed that only the type size medium sand and P19, indicating a particle size of the coarse type. The small correlation of organic matter with the elements studied in this work suggests that these are not of anthropogenic origin but geochemical support

Endogamia em um rebanho da raça Guzerá

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

La mortalidad en los primeros años de vida en países de la América Latina: Colombia 1968-1969

Incluye Bibliografía

Genetic parameters and principal component analysis for egg production from White Leghorn hens

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Informe de Guatemala

Incluye Bibliografía

Estado de Santa Catarina-Brasil: tabla de mortalidad intercensal 1960-1970. Trabajo de investigacion

Incluye Bibliografía