Produção de fitases e proteases fúngicas, aplicação em rações e valores digestíveis pela tilápia-do-nilo

Pós-graduação em Zootecnia - FMVZ

Ecological optimization of an irreversible Brayton cycle with regeneration, inter-cooling and reheating

A mathematical model is developed for an irreversible Brayton cycle with regeneration, inter-cooling and reheating. The irreversibility are from the thermal resistance in the heat exchangers, the pressure drops in pipes, the non-isentropic behavior in the adiabatic expansions and compressions and the heat leakage to the cold source. The cycle is optimized by maximizing the ecological function, which is achieved by the search for optimal values for the temperatures of the cycle and for the pressure ratios of the first stage compression and the first stage expansion. The advantages of using the regenerator, intercooler and reheater are presented by comparison with cycles that do not incorporate one or more of these processes. Optimization results are compared with those obtained by maximizing the power output and it is concluded that the point of maximum ecological function has major advantages with respect to the entropy generation rate and the thermal efficiency, at the cost of a small loss in power.

Kinetic and thermodynamic investigation of Arthrospira (Spirulina) platensis fed-batch cultivation in a tubular photobioreactor using urea as nitrogen source

BACKGROUND: Fed-batch culture allows the cultivation of Arthrospira platensis using urea as nitrogen source. Tubular photobioreactors substantially increase cell growth, but the successful use of this cheap nitrogen source requires a knowledge of the kinetic and thermodynamic parameters of the process. This work aims at identifying the effect of two independent variables, temperature (T) and urea daily molar flow-rate (U), on cell growth, biomass composition and thermodynamic parameters involved in this photosynthetic cultivation. RESULTS: The optimal values obtained were T = 32 degrees C and U = 1.16 mmol L-1 d-1, under which the maximum cell concentration was 4186 +/- 39 mg L-1, cell productivity 541 +/- 5 mg L-1 d-1 and yield of biomass on nitrogen 14.3 +/- 0.1 mg mg-1. Applying an Arrhenius-type approach, the thermodynamic parameters of growth (?H* = 98.2 kJ mol-1; ?S* = - 0.020 kJ mol-1 K-1; ?G* = 104.1 kJ mol-1) and its thermal inactivation (Delta H-D(0) =168.9 kJ mol-1; Delta S-D(0) = 0.459 kJ mol-1 K-1; Delta G(D)(0) =31.98 kJ mol-1) were estimated. CONCLUSIONS: To maximize cell growth T and U were simultaneously optimized. Biomass lipid content was not influenced by the experimental conditions, while protein content was dependent on both independent variables. Using urea as nitrogen source prevented the inhibitory effect already observed with ammonium salts. Copyright (c) 2012 Society of Chemical Industry

Probabilistic failure modelling of reinforced concrete structures subjected to chloride penetration

Structural durability is an important criterion that must be evaluated for every type of structure. Concerning reinforced concrete members, chloride diffusion process is widely used to evaluate durability, especially when these structures are constructed in aggressive atmospheres. The chloride ingress triggers the corrosion of reinforcements; therefore, by modelling this phenomenon, the corrosion process can be better evaluated as well as the structural durability. The corrosion begins when a threshold level of chloride concentration is reached at the steel bars of reinforcements. Despite the robustness of several models proposed in literature, deterministic approaches fail to predict accurately the corrosion time initiation due the inherent randomness observed in this process. In this regard, structural durability can be more realistically represented using probabilistic approaches. This paper addresses the analyses of probabilistic corrosion time initiation in reinforced concrete structures exposed to chloride penetration. The chloride penetration is modelled using the Fick's diffusion law. This law simulates the chloride diffusion process considering time-dependent effects. The probability of failure is calculated using Monte Carlo simulation and the first order reliability method, with a direct coupling approach. Some examples are considered in order to study these phenomena. Moreover, a simplified method is proposed to determine optimal values for concrete cover.

Reliability algorithms applied to reinforced concrete structures durability assessment

This paper addresses the analysis of probabilistic corrosion time initiation in reinforced concrete structures exposed to ions chloride penetration. Structural durability is an important criterion which must be evaluated in every type of structure, especially when these structures are constructed in aggressive atmospheres. Considering reinforced concrete members, chloride diffusion process is widely used to evaluate the durability. Therefore, at modelling this phenomenon, corrosion of reinforcements can be better estimated and prevented. These processes begin when a threshold level of chlorides concentration is reached at the steel bars of reinforcements. Despite the robustness of several models proposed in the literature, deterministic approaches fail to predict accurately the corrosion time initiation due to the inherently randomness observed in this process. In this regard, the durability can be more realistically represented using probabilistic approaches. A probabilistic analysis of ions chloride penetration is presented in this paper. The ions chloride penetration is simulated using the Fick's second law of diffusion. This law represents the chloride diffusion process, considering time dependent effects. The probability of failure is calculated using Monte Carlo simulation and the First Order Reliability Method (FORM) with a direct coupling approach. Some examples are considered in order to study these phenomena and a simplified method is proposed to determine optimal values for concrete cover.

Hierarchical Approach for Survivable Network Design

A central design challenge facing network planners is how to select a cost-effective network configuration that can provide uninterrupted service despite edge failures. In this paper, we study the Survivable Network Design (SND) problem, a core model underlying the design of such resilient networks that incorporates complex cost and connectivity trade-offs. Given an undirected graph with specified edge costs and (integer) connectivity requirements between pairs of nodes, the SND problem seeks the minimum cost set of edges that interconnects each node pair with at least as many edge-disjoint paths as the connectivity requirement of the nodes. We develop a hierarchical approach for solving the problem that integrates ideas from decomposition, tabu search, randomization, and optimization. The approach decomposes the SND problem into two subproblems, Backbone design and Access design, and uses an iterative multi-stage method for solving the SND problem in a hierarchical fashion. Since both subproblems are NP-hard, we develop effective optimization-based tabu search strategies that balance intensification and diversification to identify near-optimal solutions. To initiate this method, we develop two heuristic procedures that can yield good starting points. We test the combined approach on large-scale SND instances, and empirically assess the quality of the solutions vis-à-vis optimal values or lower bounds. On average, our hierarchical solution approach generates solutions within 2.7% of optimality even for very large problems (that cannot be solved using exact methods), and our results demonstrate that the performance of the method is robust for a variety of problems with different size and connectivity characteristics.

Precise pose recovery of distal locking holes from single calibrated fluoroscopic image via a novel variable decomposition approach

This paper presents a novel variable decomposition approach for pose recovery of the distal locking holes using single calibrated fluoroscopic image. The problem is formulated as a model-based optimal fitting process, where the control variables are decomposed into two sets: (a) the angle between the nail axis and its projection on the imaging plane, and (b) the translation and rotation of the geometrical model of the distal locking hole around the nail axis. By using an iterative algorithm to find the optimal values of the latter set of variables for any given value of the former variable, we reduce the multiple-dimensional model-based optimal fitting problem to a one-dimensional search along a finite interval. We report the results of our in vitro experiments, which demonstrate that the accuracy of our approach is adequate for successful distal locking of intramedullary nails.

Landscape connectivity, habitat structure and activity of bat guilds in farmland-dominated matrices

Agricultural intensification has caused a decline in structural elements in European farmland, where natural habitats are increasingly fragmented. The loss of habitat structures has a detrimental effect on biodiversity and affects bat species that depend on vegetation structures for foraging and commuting. We investigated the impact of connectivity and configuration of structural landscape elements on flight activity, species richness and diversity of insectivorous bats and distinguished three bat guilds according to species-specific bioacoustic characteristics. We tested whether bats with shorter-range echolocation were more sensitive to habitat fragmentation than bats with longer-range echolocation. We expected to find different connectivity thresholds for the three guilds and hypothesized that bats prefer linear over patchy landscape elements. Bat activity was quantified using repeated acoustic monitoring in 225 locations at 15 study plots distributed across the Swiss Central Plateau, where connectivity and the shape of landscape elements were determined by spatial analysis (GIS). Spectrograms of bat calls were assigned to species with the software batit by means of image recognition and statistical classification algorithms. Bat activity was significantly higher around landscape elements compared to open control areas. Short- and long-range echolocating bats were more active in well-connected landscapes, but optimal connectivity levels differed between the guilds. Species richness increased significantly with connectivity, while species diversity did not (Shannon's diversity index). Total bat activity was unaffected by the shape of landscape elements. Synthesis and applications. This study highlights the importance of connectivity in farmland landscapes for bats, with shorter-range echolocating bats being particularly sensitive to habitat fragmentation. More structurally diverse landscape elements are likely to reduce population declines of bats and could improve conditions for other declining species, including birds. Activity was highest around optimal values of connectivity, which must be evaluated for the different guilds and spatially targeted for a region's habitat configuration. In a multi-species approach, we recommend the reintroduction of structural elements to increase habitat heterogeneity should become part of agri-environment schemes.

Sistema de instrumentação e controle para células a combustível

Este trabajo presenta un estudio sobre el funcionamiento y aplicaciones de las células de combustible de membrana tipo PEM, o de intercambio de protones, alimentadas con hidrógeno puro y oxigeno obtenido de aire comprimido. Una vez evaluado el proceso de dichas células y las variables que intervienen en el mismo, como presión, humedad y temperatura, se presenta una variedad de métodos para la instrumentación de tales variables así como métodos y sistemas para la estabilidad y control de las mismas, en torno a los valores óptimos para una mayor eficacia en el proceso. Tomando como variable principal a controlar la temperatura del proceso, y exponiendo los valores concretos en torno a 80 grados centígrados entre los que debe situarse, es realizado un modelo del proceso de calentamiento y evolución de la temperatura en función de la potencia del calentador resistivo en el dominio de la frecuencia compleja, y a su vez implementado un sistema de medición mediante sensores termopar de tipo K de respuesta casi lineal. La señal medida por los sensores es amplificada de manera diferencial mediante amplificadores de instrumentación INA2126, y es desarrollado un algoritmo de corrección de error de unión fría (error producido por la inclusión de nuevos metales del conector en el efecto termopar). Son incluidos los datos de test referentes al sistema de medición de temperatura , incluyendo las desviaciones o error respecto a los valores ideales de medida. Para la adquisición de datos y implementación de algoritmos de control, es utilizado un PC con el software Labview de National Instruments, que permite una programación intuitiva, versátil y visual, y poder realizar interfaces de usuario gráficas simples. La conexión entre el hardware de instrumentación y control de la célula y el PC se realiza mediante un interface de adquisición de datos USB NI 6800 que cuenta con un amplio número de salidas y entradas analógicas. Una vez digitalizadas las muestras de la señal medida, y corregido el error de unión fría anteriormente apuntado, es implementado en dicho software un controlador de tipo PID ( proporcional-integral-derivativo) , que se presenta como uno de los métodos más adecuados por su simplicidad de programación y su eficacia para el control de este tipo de variables. Para la evaluación del comportamiento del sistema son expuestas simulaciones mediante el software Matlab y Simulink determinando por tanto las mejores estrategias para desarrollar el control PID, así como los posibles resultados del proceso. En cuanto al sistema de calentamiento de los fluidos, es empleado un elemento resistor calentador, cuya potencia es controlada mediante un circuito electrónico compuesto por un detector de cruce por cero de la onda AC de alimentación y un sistema formado por un elemento TRIAC y su circuito de accionamiento. De manera análoga se expone el sistema de instrumentación para la presión de los gases en el circuito, variable que oscila en valores próximos a 3 atmosferas, para ello es empleado un sensor de presión con salida en corriente mediante bucle 4-20 mA, y un convertidor simple corriente a tensión para la entrada al sistema de adquisición de datos. Consecuentemente se presenta el esquema y componentes necesarios para la canalización, calentamiento y humidificación de los gases empleados en el proceso así como la situación de los sensores y actuadores. Por último el trabajo expone la relación de algoritmos desarrollados y un apéndice con información relativa al software Labview. ABTRACT This document presents a study about the operation and applications of PEM fuel cells (Proton exchange membrane fuel cells), fed with pure hydrogen and oxygen obtained from compressed air. Having evaluated the process of these cells and the variables involved on it, such as pressure, humidity and temperature, there is a variety of methods for implementing their control and to set up them around optimal values for greater efficiency in the process. Taking as primary process variable the temperature, and exposing its correct values around 80 degrees centigrade, between which must be placed, is carried out a model of the heating process and the temperature evolution related with the resistive heater power on the complex frequency domain, and is implemented a measuring system with thermocouple sensor type K performing a almost linear response. The differential signal measured by the sensor is amplified through INA2126 instrumentation amplifiers, and is developed a cold junction error correction algorithm (error produced by the inclusion of additional metals of connectors on the thermocouple effect). Data from the test concerning the temperature measurement system are included , including deviations or error regarding the ideal values of measurement. For data acquisition and implementation of control algorithms, is used a PC with LabVIEW software from National Instruments, which makes programming intuitive, versatile, visual, and useful to perform simple user interfaces. The connection between the instrumentation and control hardware of the cell and the PC interface is via a USB data acquisition NI 6800 that has a large number of analog inputs and outputs. Once stored the samples of the measured signal, and correct the error noted above junction, is implemented a software controller PID (proportional-integral-derivative), which is presented as one of the best methods for their programming simplicity and effectiveness for the control of such variables. To evaluate the performance of the system are presented simulations using Matlab and Simulink software thereby determining the best strategies to develop PID control, and possible outcomes of the process. As fluid heating system, is employed a heater resistor element whose power is controlled by an electronic circuit comprising a zero crossing detector of the AC power wave and a system consisting of a Triac and its drive circuit. As made with temperature variable it is developed an instrumentation system for gas pressure in the circuit, variable ranging in values around 3 atmospheres, it is employed a pressure sensor with a current output via 4-20 mA loop, and a single current to voltage converter to adequate the input to the data acquisition system. Consequently is developed the scheme and components needed for circulation, heating and humidification of the gases used in the process as well as the location of sensors and actuators. Finally the document presents the list of algorithms and an appendix with information about Labview software.

Estudio Acústico del Teatro Tomás y Valiente de Fuenlabrada

El objetivo de este Proyecto Fin de Carrera es realizar el estudio de las características acústicas del Teatro Tomás y Valiente de Fuenlabrada mediante medidas y con el apoyo de los resultados obtenidos mediante la simulación del campo sonoro. El recinto está destinado principalmente a representaciones teatrales, empleándose también como sala polivalente, de forma que se analizará su comportamiento acústico e idoneidad ante la variedad de usos a los que se destina. Para ello, se realizan medidas experimentales in situ de todos los parámetros representativos de un recinto acústico y la predicción de los mismos mediante la simulación de la sala a través del software de simulación acústica EASE, de forma que las características acústicas obtenidas mediante ambos procesos sean comparadas proponiéndose mejoras en el entorno para cumplimiento de parámetros acústicos óptimos exigibles a la sala. En primer lugar se exponen los principales conceptos teóricos a tener en cuenta en el ámbito de la acústica, detallando las diferentes teorías de estudio, los principios básicos de la psicoacústica. Además, se definen los criterios utilizados en el diseño de recintos acústicos y parámetros que definan la calidad según el uso al que se destine en función del estudio de la utilización habitual de la sala y valores óptimos de los parámetros acústicos correspondientes a salas de tamaño y uso similar. A continuación, se describe la metodología aplicada para la realización de las medidas in situ obteniendo resultados de los parámetros acústicos representativos del recinto para el análisis de sus características acústicas y posterior comparación con la predicción de los mismos mediante la simulación del modelo informático. También se muestra el proceso que se ha seguido para el diseño del modelo acústico a partir de los planos del teatro y medidas realizadas en el recinto, para la simulación de parámetros y características acústicas. Finalmente se exponen las conclusiones extraídas tras el estudio realizado y la propuesta de mejoras en el entorno para cumplimiento de parámetros acústicos óptimos que se puedan exigir a esta sala, incluyendo un presupuesto que muestre la viabilidad económica del proyecto. ABSTRACT. The goal of this final project, is to perform an acoustic study and simulation of the Tomás y Valiente theatre in Fuenlabrada. These premises are mainly used for stage plays, but also as a multipurpose space, therefore its acustic behaviour and suitability for the expected uses will be analyzed. To accomplish this task, experimental measures for all the representative parameters for an acoustic hall, will be taken on site. The prediction for those measurements will be simulated through EASE software, so the acoustic characteristics obtained using both methods will be compared, and improvements will be proposed in order to achieve the best acoustic parameters, the hall can have. First at all, the theoretical concepts definition involves exposing the main concepts to consider in the acoustics field, detailing the basic principles of the psychoacoustic. On top of the criteria used in the design of acoustic enclosures and parameters defining the quality according to the use the enclosure is intended for. Research on the most common usage for the space, and optimal values, comparing it with similar rooms in size and use. Experimental measures are made of the acoustic parameters representative of the enclosure for the analysis of its acoustic characteristics and its later comparison with the prediction of the parameters through informatics model simulation. Also the process which has been followed for the design of acoustic model of the theater are taken from on site measurements, experimental representative measures and acoustic parameters, for the acoustic characteristics analysis and post comparison with the software model simulation and prediction. Acoustic design of the theater taking as a base the building blueprints, and manual measures, for the parameters and acoustic characteristics simulation. Finally, the conclusions extracted after the performed research are shown and the propose of improvements in the environment for fulfillment of acoustic optimal parameters which can be required to this room, including a quote with shows the economical viability of the project.

Desarrollo de conglomerados con la incorporación de cenizas volantes procedentes de la incineración de residuos sólidos urbanos

Esta tesis presenta los resultados de la investigación realizada sobre la inertización de cenizas volantes procedentes de residuos sólidos urbanos y su posterior encapsulación en distintas matrices de mortero. Durante el proceso de inertización, se ha logrado la inertización de éste residuo tóxico y peligroso (RTP) y también su valorización como subproducto. De esta forma se dispone de nueva “materia prima” a bajo coste y la eliminación de un residuo tóxico y peligroso con la consiguiente conservación de recursos naturales alternativos. La caracterización química de las cenizas analizadas refleja que éstas presentan altas concentraciones de cloruros, Zn y Pb. Durante la investigación se ha desarrollado un proceso de inertización de las cenizas volantes con bicarbonato sódico (NaHCO3) que reduce en un 99% el contenido en cloruros y mantiene el pH en valores óptimos para que la concentración de los metales pesados en el lixiviado sea mínima debido a su estabilización en forma de carbonatos insolubles. Se han elaborado morteros con cuatro tipos distintos de cementos (CEM-I, CEM-II, CAC y CSA) incorporando cenizas volantes inertizadas en una proporción igual a un 10% en peso del árido utilizado. Los morteros ensayados abarcan distintas dosificaciones tanto en la utilización de áridos con distintos diámetros (0/2 y 0/4), como en la relación cemento/árido (1/1 y 1/3). Se han obtenido las propiedades físicas y mecánicas de estos morteros mediante ensayos de Trabajabilidad, Estabilidad Dimensional, Carbonatación, Porosidad y Resistencias Mecánicas. De igual forma, se presentan resultados de ensayos de lixiviación de Zn, Pb, Cu y Cd, sobre probetas monolíticas de los morteros con los mejores comportamientos físico/mecánicos, donde se ha analizado el contenido en iones de dichos metales pesados lixiviados mediante determinación voltamperométrica de redisolución anódica Se concluye que todos los morteros ensayados son técnicamente aceptables, siendo los más favorables los elaborados con Cemento de Sulfoaluminato de Calcio (CSA) y con Cemento de Aluminato de Calcio (CAC). En este último caso, se mejoran las resistencias a compresión de los morteros de referencia en más de un 48%, y las resistencias a flexión en más de un 67%. De igual forma, los ensayos de lixiviado revelan la completa encapsulación de los iones de Zn y la mitigación en el lixiviado de los iones de Pb. Ambos morteros podrían ser perfectamente validos en actuaciones en las que se necesitase un producto de fraguado rápido, altas resistencias iniciales y compensación de las retracciones con una elevada estabilidad dimensional. En base a esto, el material podría ser utilizado como mortero de reparación en viales y pavimentos que requiriesen altas prestaciones, tales como: soleras industriales, pistas de aterrizaje, aparcamientos, etc. O bien, para la confección de elementos prefabricados sin armaduras estructurales, dada su elevada resistencia a flexión. ABSTRACT This dissertation presents the results of a research on inerting fly ash from urban solid waste and its subsequent encapsulation in mortar matrixes. The inerting of this hazardous toxic waste, as well as its valorization as a by-product has been achieved. In this way, a new "raw material" is available through a simple process and the toxic and hazardous waste is eliminated, and consequently, conservation of alternative natural resources is strengthened. Chemical analysis of the ashes analyzed shows high concentrations of soluble chlorides, Zn and Pb. An inerting process of fly ash with sodium bicarbonate (NaHCO3) has been developed which reduces 99% the content of chlorides and maintains pH at optimal values, so that the concentration of heavy metals in the leachate is minimum, due to its stabilization in the form of insoluble carbonates. Mortars with four different types of cements (CEM-I, CEM-II, CAC and CSA) have been developed by the addition of inertized fly ash in the form of carbonates, in the proportion of 10% in weight of the aggregates used. The samples tested include different proportions in the use of aggregates with different sizes (0/2 and 0/4), and in the cement/aggregate ratio (1/1 and 1/3). Physical/mechanical properties of these mortars have been studied through workability, dimensional stability, carbonation, porosity and mechanic strength tests. Leaching tests of Zn, Pb, Cu and Cd ions are also being performed on monolithic samples of the best behavioral mortars. The content in leachated heavy metal ions is being analyzed through stripping voltammetry determination. Conclusions drawn are that the tested CAC and CSA cement mortars present much better behavior than those of CEM-I and CEM-II cement. The results are especially remarkable for the CAC cement mortars, improving reference mortars compression strengths in more than 48%, and also bending strengths in more than 67%. Leaching tests confirm that the encapsulation of Zn and Pb is achieved and leachate of both ions is mitigated within the mortar matrixes. For the above stated reasons, it might be concluded that mortars made with calcium aluminate cements or calcium sulfoaluminate with the incorporation of treated fly ash, may be perfectly valid for uses in which a fast-curing product, with high initial strength and drying shrinkage compensation with a high dimensional stability is required. Based on this, the material could be used as repair mortar for structures, roads and industrial pavements requiring high performance, such as: industrial floorings, landing tracks, parking lots, etc. Alternatively, it could also be used in the manufacture of prefabricated elements without structural reinforcement, given its high bending strength.

Ajuste del modelo de Drucker Prager extendido para la simulación de la respuesta mecánica de un vertisol mediante el método de elementos finitos

La mecanización de las labores del suelo es la causa, por su consumo energético e impacto directo sobre el medio ambiente, que más afecta a la degradación y pérdida de productividad de los suelos. Entre los factores de disminución de la productividad se deben considerar la compactación, la erosión, el encostramiento y la pérdida de estructura. Todo esto obliga a cuidar el manejo agrícola de los suelos tratando de mejorar las condiciones del suelo y elevar sus rendimientos sin comprometer aspectos económicos, ecológicos y ambientales. En el presente trabajo se adecuan los parámetros constitutivos del modelo de Drucker Prager Extendido (DPE) que definen la fricción y la dilatancia del suelo en la fase de deformación plástica, para minimizar los errores en las predicciones durante la simulación de la respuesta mecánica de un Vertisol mediante el Método de Elementos Finitos. Para lo cual inicialmente se analizaron las bases teóricas que soportan este modelo, se determinaron las propiedades y parámetros físico-mecánicos del suelo requeridos como datos de entrada por el modelo, se determinó la exactitud de este modelo en las predicciones de la respuesta mecánica del suelo, se estimaron mediante el método de aproximación de funciones de Levenberg-Marquardt los parámetros constitutivos que definen la trayectoria de la curva esfuerzo-deformación plástica. Finalmente se comprobó la exactitud de las predicciones a partir de las adecuaciones realizadas al modelo. Los resultados permitieron determinar las propiedades y parámetros del suelo, requeridos como datos de entrada por el modelo, mostrando que su magnitud está en función su estado de humedad y densidad, además se obtuvieron los modelos empíricos de estas relaciones exhibiendo un R2>94%. Se definieron las variables que provocan las inexactitudes del modelo constitutivo (ángulo de fricción y dilatancia), mostrando que las mismas están relacionadas con la etapa de falla y deformación plástica. Finalmente se estimaron los valores óptimos de estos ángulos, disminuyendo los errores en las predicciones del modelo DPE por debajo del 4,35% haciéndelo adecuado para la simulación de la respuesta mecánica del suelo investigado. ABSTRACT The mechanization using farming techniques is one of the main factors that affects the most the soil, causing its degradation and loss of productivity, because of its energy consumption and direct impact on the environment. Compaction, erosion, crusting and loss of structure should be considered among the factors that decrease productivity. All this forces the necessity to take care of the agricultural-land management trying to improve soil conditions and increase yields without compromising economic, ecological and environmental aspects. The present study was aimed to adjust the parameters of the Drucker-Prager Extended Model (DPE), defining friction and dilation of soil in plastic deformation phase, in order to minimize the error of prediction when simulating the mechanical response of a Vertisol through the fine element method. First of all the theoretic fundamentals that withstand the model were analyzed. The properties and physical-mechanical parameters of the soil needed as input data to initialize the model, were established. And the precision of the predictions for the mechanical response of the soil was assessed. Then the constitutive parameters which define the path of the plastic stress-strain curve were estimated through Levenberg-Marquardt method of function approximations. Lastly the accuracy of the predictions from the adequacies made to the model was tested. The results permitted to determine those properties and parameters of the soil, needed in order to initialize the model. It showed that their magnitude is in function of density and humidity. Moreover, the empirical models from these relations were obtained: R2>94%. The variables producing inaccuracies in the constitutive model (angle of repose and dilation) were defined, and there was showed that they are linked with the plastic deformation and rupture point. Finally the optimal values of these angles were established, obtaining thereafter error values for the DPE model under 4, 35%, and making it suitable for the simulation of the mechanical response of the soil under study.

A resistance emulation approach to optimize the wave energy harvesting for a direct drive point absorber

In general, a major challenge for the exploitation of renewable energies is to improve their efficiency. In electricity generation from the energy of ocean waves, not unlike other technologies, the converter must be optimized to make the energy harvesting economically feasible. This paper proposes a passive tuning control strategy of a point absorber in which the power captured is maximized by controlling the electromagnetic force of the generator with a resistance emulation approach. The proposed strategy consists of mapping the optimal values for regular waves and applying them to irregular waves. This strategy is tested in a wave energy converter in which the generator is connected to a boost rectifier converter whose controller is designed to emulate a resistance. The power electronics system implemented is validated by comparing its performance with the case in which the generator is directly connected to a resistive load. The simulation results show the effectiveness of the proposed strategy as the maximum captured power is concentrated around the optimal values previously calculated and with the same behavior for both excitations.

PCR candidate region mismatch scanning: adaptation to quantitative, high-throughput genotyping

Linkage and association analyses were performed to identify loci affecting disease susceptibility by scoring previously characterized sequence variations such as microsatellites and single nucleotide polymorphisms. Lack of markers in regions of interest, as well as difficulty in adapting various methods to high-throughput settings, often limits the effectiveness of the analyses. We have adapted the Escherichia coli mismatch detection system, employing the factors MutS, MutL and MutH, for use in PCR-based, automated, high-throughput genotyping and mutation detection of genomic DNA. Optimal sensitivity and signal-to-noise ratios were obtained in a straightforward fashion because the detection reaction proved to be principally dependent upon monovalent cation concentration and MutL concentration. Quantitative relationships of the optimal values of these parameters with length of the DNA test fragment were demonstrated, in support of the translocation model for the mechanism of action of these enzymes, rather than the molecular switch model. Thus, rapid, sequence-independent optimization was possible for each new genomic target region. Other factors potentially limiting the flexibility of mismatch scanning, such as positioning of dam recognition sites within the target fragment, have also been investigated. We developed several strategies, which can be easily adapted to automation, for limiting the analysis to intersample heteroduplexes. Thus, the principal barriers to the use of this methodology, which we have designated PCR candidate region mismatch scanning, in cost-effective, high-throughput settings have been removed.

Adsorption of argon and nitrogen in cylindrical pores of MCM-41 materials: application of density functional theory

Adsorption of argon and nitrogen at their respective boiling points in cylindrical pores of MCM-41 type silica-like adsorbents is studied by means of a non-local density functional theory (NLDFT), which is modified to deal with amorphous solids. By matching the theoretical results of the pore filling pressure versus pore diameter against the experimental data, we arrive at a conclusion that the adsorption branch (rather than desorption) corresponds to the true thermodynamic equilibrium. If this is accepted, we derive the optimal values for the solid–fluid molecular parameters for the system amorphous silica–Ar and amorphous silica–N2, and at the same time we could derive reliably the specific surface area of non-porous and mesoporous silica-like adsorbents, without a recourse to the BET method. This method is then logically extended to describe the local adsorption isotherms of argon and nitrogen in silica-like pores, which are then used as the bases (kernel) to determine the pore size distribution. We test this with a number of adsorption isotherms on the MCM-41 samples, and the results are quite realistic and in excellent agreement with the XRD results, justifying the approach adopted in this paper.