Kinetic and thermodynamic investigation of Arthrospira (Spirulina) platensis fed-batch cultivation in a tubular photobioreactor using urea as nitrogen source
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
23/09/2013
23/09/2013
01/11/2012
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Resumo |
BACKGROUND: Fed-batch culture allows the cultivation of Arthrospira platensis using urea as nitrogen source. Tubular photobioreactors substantially increase cell growth, but the successful use of this cheap nitrogen source requires a knowledge of the kinetic and thermodynamic parameters of the process. This work aims at identifying the effect of two independent variables, temperature (T) and urea daily molar flow-rate (U), on cell growth, biomass composition and thermodynamic parameters involved in this photosynthetic cultivation. RESULTS: The optimal values obtained were T = 32 degrees C and U = 1.16 mmol L-1 d-1, under which the maximum cell concentration was 4186 +/- 39 mg L-1, cell productivity 541 +/- 5 mg L-1 d-1 and yield of biomass on nitrogen 14.3 +/- 0.1 mg mg-1. Applying an Arrhenius-type approach, the thermodynamic parameters of growth (?H* = 98.2 kJ mol-1; ?S* = - 0.020 kJ mol-1 K-1; ?G* = 104.1 kJ mol-1) and its thermal inactivation (Delta H-D(0) =168.9 kJ mol-1; Delta S-D(0) = 0.459 kJ mol-1 K-1; Delta G(D)(0) =31.98 kJ mol-1) were estimated. CONCLUSIONS: To maximize cell growth T and U were simultaneously optimized. Biomass lipid content was not influenced by the experimental conditions, while protein content was dependent on both independent variables. Using urea as nitrogen source prevented the inhibitory effect already observed with ammonium salts. Copyright (c) 2012 Society of Chemical Industry CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Sao Paulo-Brazil CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Sao PauloBrazil |
Identificador |
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, HOBOKEN, v. 87, n. 11, pp. 1574-1583, NOV, 2012 0268-2575 http://www.producao.usp.br/handle/BDPI/33594 10.1002/jctb.3795 |
Idioma(s) |
eng |
Publicador |
WILEY-BLACKWELL HOBOKEN |
Relação |
Journal of Chemical Technology and Biotechnology |
Direitos |
openAccess Copyright WILEY-BLACKWELL |
Palavras-Chave | #ARTHROSPIRA (SPIRULINA) PLATENSIS #BIOMASS COMPOSITION #FED-BATCH PROCESS #KINETICS AND THERMODYNAMICS #TUBULAR PHOTOBIOREACTOR #UREA #GREEN-ALGA SPIRULINA #AMMONIUM-CHLORIDE #LIGHT-INTENSITY #TEMPERATURE #GROWTH #FERMENTATION #MICROALGAE #CHLOROPHYLL #SULFATE #MAXIMA #BIOTECHNOLOGY & APPLIED MICROBIOLOGY #CHEMISTRY, MULTIDISCIPLINARY #ENGINEERING, CHEMICAL |
Tipo |
article original article publishedVersion |