NF-κB-dependent inhibition of apoptosis is essential for host cell survival during Rickettsia rickettsii infection

The possibility that bacteria may have evolved strategies to overcome host cell apoptosis was explored by using Rickettsia rickettsii, an obligate intracellular Gram-negative bacteria that is the etiologic agent of Rocky Mountain spotted fever. The vascular endothelial cell, the primary target cell during in vivo infection, exhibits no evidence of apoptosis during natural infection and is maintained for a sufficient time to allow replication and cell-to-cell spread prior to eventual death due to necrotic damage. Prior work in our laboratory demonstrated that R. rickettsii infection activates the transcription factor NF-κB and alters expression of several genes under its control. However, when R. rickettsii-induced activation of NF-κB was inhibited, apoptosis of infected but not uninfected endothelial cells rapidly ensued. In addition, human embryonic fibroblasts stably transfected with a superrepressor mutant inhibitory subunit IκB that rendered NF-κB inactivatable also underwent apoptosis when infected, whereas infected wild-type human embryonic fibroblasts survived. R. rickettsii, therefore, appeared to inhibit host cell apoptosis via a mechanism dependent on NF-κB activation. Apoptotic nuclear changes correlated with presence of intracellular organisms and thus this previously unrecognized proapoptotic signal, masked by concomitant NF-κB activation, likely required intracellular infection. Our studies demonstrate that a bacterial organism can exert an antiapoptotic effect, thus modulating the host cell’s apoptotic response to its own advantage by potentially allowing the host cell to remain as a site of infection.

Phylogenetic footprinting of transcription factor binding sites in proteobacterial genomes

Toward the goal of identifying complete sets of transcription factor (TF)-binding sites in the genomes of several gamma proteobacteria, and hence describing their transcription regulatory networks, we present a phylogenetic footprinting method for identifying these sites. Probable transcription regulatory sites upstream of Escherichia coli genes were identified by cross-species comparison using an extended Gibbs sampling algorithm. Close examination of a study set of 184 genes with documented transcription regulatory sites revealed that when orthologous data were available from at least two other gamma proteobacterial species, 81% of our predictions corresponded with the documented sites, and 67% corresponded when data from only one other species were available. That the remaining predictions included bona fide TF-binding sites was proven by affinity purification of a putative transcription factor (YijC) bound to such a site upstream of the fabA gene. Predicted regulatory sites for 2097 E.coli genes are available at http://www.wadsworth.org/resnres/bioinfo/.

Staatkundige geschriften, Opgesteld en nagelaaten

Bound in half contemporary calf with title in gilt on spine.

School of Nursing Class of 1976

Top Row: Peggy Alford, Anne Allen, Barbara Amann, Joann Baker, Elizabeth Bando, Sara Barnes, Mary Barz, Anne Bauer, Patricia Baxter, Gwendolyn Beaudette, Nancy Bidigare, Marian Bier, Susan Blaszczyk, Jacqueline bolin, Anne Bostwick, Heather Brendel, Theresa Brisker, Rosalyn Cage, Jeanne Calhoun

Row 2: Karen Caputo, Carolyn Carpenter, Laura Lewellen, Catherine Pearson, Pamela Carr, Lisa Clark

Row 3: Sarah Cleland, Kathy Collar, Ann Connors, Marian Coppo, Martha Coppo, Debra Wirth, Kathleen Coughlin, Mayble Craig, Jennifer Crittenden, Linda Dean, Ann Dika

Row 4: Shira Doneson, Valerie Dray, Lee Duffey, Mary Dunn, Nancy Edwards, Mildred Jett, Patricia Johnson, Jan Walters, Karen Dimitroff, Debra Walton, Moira Stein, Madi Ehrenreich, Paula Elliott, Claudia Evans, Jolaync Farrell, Karen Fierke

Row 5: Debra Finch, Nadine Furlong, Susan Gamerman, Anita Gardocki, Marcia Gerpheide, Roberta Gies, Deborah Glotzhober, Marlene Golabeck, Janet Goldberg, Rene Green, Diana Greer, Susan Gross, Vivian Hall, Jill Hallead

Row 6: Sharon Hamlett, Tamara Hanson, Jane Harper, Jesusa Heilig, Steinunn Hermannsson, Susan Hicks, Karen Hillebrand, Jomatia Hoff, Michelle Howey, Holly Howieson, Sandra Hubar, Kathleen Hughes, Shirley Jvery, Laura Johnson, Susan Johnson, Shirley Jones, Judith Kellermier, Lynda Kitchen, Susan Kleinbeck

Row 7: Nanette Kotz, Kathleen Kroh, Judith Krohn, Catherine Lahti, Mary Lange, Patti Larson, Susan Leach, Rebecca Linn, Lacy Loomis, Francene Lundy, Sue Lymperis, Robyn Main, Patricia McCleary, Theresa McGowan, Elizabeth Messiter, Mary Miller, Nancy Moffatt, Catherine Munn, Karen Munson

Row 8: Virginia Newman, Laura Novak, Thomas O'Connell, Julie O'Connor, Kaathleen O'Hara, Kimberly O'Loughlin, Karen Olsen, Marcy Ouellette, Gail Park, Georgiana Parsell, Mary Patchak, Linda Pearsall, Kathleen Poage, Shelly Ponte, Thomas Parter, Marilyn Pratt, Karen Prince, Kathryn Procter, Rebecca Raymond

Row 9: Jill remter, Cheryl Ricca, Brenda Robinson, Karen Rollins, Lisa Root, Audrey Ross, Barbara Rutherford, Linda Rykwalder, Margaret Sampson, Sherril Santo, Jeanne Scheer, Kathy Schlichter, Nancy Schuman, Debra Sihtala, Michele Smit, Donna Smith, Bonnic Smrcka, Janine Speck, Elizabeth Stainsby

Row 10: Grace Steinaway, Jennifer Stinson, Sally Stone, Anne Sullivan, Barbara Tonak, Linda Towers, Cindy Tremblay, Gregory Trowbridge, Sandra Tucker, Debbie Ullrich, Lee Ann Van Houten, Pamela Waggener, Martha Walker, Michele Wenderski, Catherine West, Harriet Wilkinson, Diane Willis, Jan Winslow, Karen Wismer

The effects of biofeedback on the occurrence of urinary incontinence in patients following radical retropubic prostatectomy

Postprostatectomy incontinence can create a significant barrier to the attainment of optimal social and physical functioning postoperatively. The objective of this retrospective, descriptive study was to determine the effect of biofeedback on the incidence of urinary incontinence in men status post radical retropubic prostatectomy (RRP). All patients of a urologic practice who had a biofeedback session preoperatively, and two sessions postoperatively, were sent a survey to complete. Correlational analysis concluded that there was no significance (p > .05) between a subject's age, educational level, or adherence to biofeedback therapy, and their level of postprostatectomy incontinence. Of those subjects who participated (n = 46), 35% reported their urinary control as excellent, 50% reported good results, and 15% reported fair results. No subjects reported poor urinary control. Ninety-five percent of subjects said they would recommend the biofeedback treatment to a friend, and 88% felt that biofeedback had helped them attain their present level of urinary control.

Rasch analysis of the routines-based interview implementation checklist

The Routines-Based Interview (RBI) is useful for developing functional outcomes/goals, for establishing strong relationships with families, and for assessing the family’s true needs. In this study, the authors investigated the psychometric properties of the RBI Implementation Checklist, conducted by 120 early intervention professionals, specifically looking at the probability of correct responses on the items as a logistic function of the difference between the person and the item parameters.We selected Rasch analysis (Rasch, 1980) for this study so we could answer questions about both how themeasure performed and how the interviewers performed and we related these performances to one another. Results indicate that scores on the RBI Implementation Checklist were reliable. The checklist could possibly benefit from more difficult items to measure the true performance of the few people who had scores higher than the most difficult items and also from additional items that focus on the family.

Assessing the role of small farms in sustainable Food and Nutrition Security: What to consider t what scale?,

The AgroMed International Conference 2016 aims to discuss the current land use changes, with a particular interest on farm and land system dynamics, also considering the possible competition with other uses (urban and/or natural land uses). It is focused on “Farm and land system dynamics in the Mediterranean basin: integrating spatial scales, from the local to the global one”. Teresa Pinto Correia presented H2020 project SALSA “Small farms, small food businesses and sustainable food security”

Shakers : compendium of the origin, history, princicples, rules and regulations, government, and doctrines of the United Society of Believers in Christ's Second Appearing : with biographies of Ann Lee, William Lee, Jas. Whittaker, J. Hocknell, J. Meacham, and Lucy Wright

by F. W. Evans

Ann Eliza Hepburne Rooth fonds, 1837-1897

Ann Eliza Hepburne was born in Chippawa, Ontario, in 1821, to William Hepburne and Susan Shannon. In 1842, she married William Anthony Rooth in St. James Cathedral in Toronto. They continued to live in different parts of the Niagara region, including Drummondville, Welland and Port Colborne. William was the editor and proprietor of the Drummondville Reporter, as well as an accountant and insurance agent, and later worked for the Customs Service in Port Colborne. He died in 1878, and Eliza in 1899. Both are buried in Drummond Hill Cemetery in Niagara Falls, Ontario.

Citología de impresión para neoplasias de la superficie ocular

Se estimó la sensibilidad y especificidad de la citología de impresión como prueba diagnóstica en lesiones conjuntivales clínicamente sospechosas de neoplasia usando como patrón de oro la patología. Se estudiaron 60 pacientes, que ingresaron al azar a la Fundación Oftalmológica Nacional, con diagnóstico clínico de neoplasia de superficie ocular o lesión sospechosa de neoplasia, quienes fueron sometidos a citología de impresión y posterior resección quirúrgica completa, más estudio patológico de la lesión. Se realizó un análisis descriptivo, analizando la sensibilidad y la especificidad con el método clásico y análisis bayesiano.

Enfermedad tiroidea autoinmune en pacientes colombianos con lupus eritematoso sistémico

Objetivos: Determinar la prevalencia y los factores asociados con el desarrollo de hipotiroidismo autoinmune (HA) en una cohorte de pacientes con lupus eritematoso sistémico (LES), y analizar la información actual en cuanto a la prevalencia e impacto de la enfermedad tiroidea autoinmune y la autoinmunidad tiroidea en pacientes con LES. Métodos: Este fue un estudio realizado en dos pasos. Primero, un total de 376 pacientes con LES fueron evaluados sistemáticamente por la presencia de: 1) HA confirmado, 2) positividad para anticuerpos tiroperoxidasa/tiroglobulina (TPOAb/TgAb) sin hipotiroidismo, 3) hipotiroidismo no autoinmune, y 4) pacientes con LES sin hipotiroidismo ni positividad para TPOAb/TgAb. Se construyeron modelos multivariados y árboles de regresión y clasificación para analizar los datos. Segundo, la información actual fue evaluada a través de una revisión sistemática de la literatura (RLS). Se siguieron las guías PRISMA para la búsqueda en las bases de datos PubMed, Scopus, SciELO y Librería Virtual en Salud. Resultados: En nuestra cohorte, la prevalencia de HA confirmado fue de 12% (Grupo 1). Sin embargo, la frecuencia de positividad para TPOAb y TgAb fue de 21% y 10%, respectivamente (Grupo 2). Los pacientes con LES sin HA, hipotiroidismo no autoinmune ni positividad para TPOAb/TgAb constituyeron el 40% de la corhorte. Los pacientes con HA confirmada fueron estadísticamente significativo de mayor edad y tuvieron un inicio tardío de la enfermedad. El tabaquismo (ORA 6.93, IC 95% 1.98-28.54, p= 0.004), la presencia de Síndrome de Sjögren (SS) (ORA 23.2, IC 95% 1.89-359.53, p= 0.015) y la positividad para anticuerpos anti-péptido cíclico citrulinado (anti-CCP) (ORA 10.35, IC 95% 1.04-121.26, p= 0.047) se asociaron con la coexistencia de LES-HA, ajustado por género y duración de la enfermedad. El tabaquismo y el SS fueron confirmados como factores predictivos para LES-HA (AUC del modelo CART = 0.72). En la RSL, la prevalencia de ETA en LES varío entre 1% al 60%. Los factores asociados con esta poliautoinmunidad fueron el género femenino, edad avanzada, tabaquismo, positividad para algunos anticuerpos, SS y el compromiso articular y cutáneo. Conclusiones: La ETA es frecuente en pacientes con LES, y no afecta la severidad del LES. Los factores de riesgo identificados ayudarán a los clínicos en la búsqueda de ETA. Nuestros resultados deben estimular políticas para la suspensión del tabaquismo en pacientes con LES.

Universa Theologia Thomistica cum propiis fontibus en ancilla philosophia...publicae offert comertationi P. Fr. Emmanuel Lazaro...adsistente...Casimiro Ignacio Ibañez...ann. MDCCLXXXIV

Sign. : []4, A-F4, G2

Oratio valedictoria: [Delivered at Harvard Commencement, 1736, before Ew. Belcher &c. With copy on blue paper.]

The notebook contains the original six-page manuscript of the valedictory address composed in Latin by graduate Edward Winslow at the 1736 Harvard Commencement enclosed within 19th century blue notebook paper on which a second handwritten copy of the address is written. The original text includes edits and struck-through words.