Genotyping of Brazilian Giardia duodenalis human axenic isolates

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da deficiência de potássio sobre as atividades de glutamato desidrogenase e glutamato sintase em feijoeiro (Phaseolus vulgaris L.)

Foi estudado o efeito da deficiência de potássio sobre as atividades da glutamato desidrogenase (EC. 1.4.1.2) e glutamato sintase (EC. 2.6.1.53) em folhas de feijoeiro (Phaseolus vulgaris L.). Os resultados mostraram que a atividade específica da glutamato desidrogenase aumentou nas plantas deficientes em potássio nos dois cultivares estudados. Foi detectada redução na atividade de glutamato sintase nas plantas deficientes em potássio.

Reação enzimática ao estresse salino durante a germinação de estilosantes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Isoenzimas no monitoramento da deterioração de sementes de Euterpe espiritosantensis Fernandes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nitrogen assimilation in Citrus based on CitEST data mining

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Comparison of hemodynamic, clinicopathologic, and gastrointestinal motility effects and recovery characteristics of anesthesia with isoflurane and halothane in horses undergoing arthroscopic surgery

Objective - To compare hemodynamic, clinicopathologic, and gastrointestinal motility effects and recovery characteristics of halothane and isoflurane in horses undergoing arthroscopic surgery. Animals - 8 healthy adult horses. Procedure - Anesthesia was maintained with isoflurane or halothane (crossover study). At 6 intervals during anesthesia and surgery, cardiopulmonary variables and related derived values were recorded. Recovery from anesthesia was assessed; gastrointestinal tract motility was subjectively monitored for 72 hours after anesthesia. Horses were administered chromium, and fecal chromium concentration was used to assess intestinal transit time. Venous blood samples were collected for clinicopathologic analyses before and 2, 24, and 48 hours after anesthesia. Results - Compared with halothane-anesthetized horses, cardiac index, oxygen delivery, and heart rate were higher and systemic vascular resistance was lower in isoflurane-anesthetized horses. Mean arterial blood pressure and the dobutamine dose required to maintain blood pressure were similar for both treatments. Duration and quality of recovery from anesthesia did not differ between treatments, although the recovery periods were somewhat shorter with isoflurane. After isoflurane anesthesia, gastrointestinal motility normalized earlier and intestinal transit time of chromium was shorter than that detected after halothane anesthesia. Compared with isoflurane, halothane was associated with increases in serum aspartate transaminase and glutamate dehydrogenase activities, but there were no other important differences in clinicopathologic variables between treatments. Conclusions and clinical relevance - Compared with halothane, isoflurane appears to be associated with better hemodynamic stability during anesthesia, less hepatic and muscle damage, and more rapid return of normal intestinal motility after anesthesia in horses undergoing arthroscopic procedures.

Epidemiologia das parasitoses intestinais e caracterização genotípica de isolados de Giardia duodenalis de escolares do município de Pratânia, estado de São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Alterações histológicas e bioquímicas e potencial fisiológico de sementes de soja

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Genotipagem dos isolados de Giardia duodenalis em famílias de pescadores da colônia de Porto Said, Botucatu, São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

GDH activity and ammonium excretion in the marine mysid, "Leptomysis lingvura": effects of age and starvation

[EN] Ammonium (NH4+) release by bacterial remineralization and heterotrophic grazers determines the regenerated fraction of phytoplankton productivity, so the measurement of NH4+ excretion in marine organisms is necessary to characterize both the magnitude and the efficiency of the nitrogen cycle. Glutamate dehydrogenase (GDH) is largely responsible for NH4+ formation in crustaceans and consequently should be useful in estimating NH4+ excretion by marine zooplankton.
Here, we address body size and starvation as sources of variability on the GDH to NH4+ excretion ratio (GDH/RNH4+). We found a strong correlation between the RNH4+ and the GDH activity (r2 = 0.87, n = 41) during growth. Since GDH activity maintained a linear relation (b = 0.93) and RNH4+ scaled exponentially (b =0.55) in well fed mysids, the GDH/RNH4+ ratio increased with size. However, the magnitude of its variation increased even more when adult mysids were starved. In this case, the GDH/RNH4+ ratio ranged from 11.23 to 102.41.

GDH activity and ammonium excretion in the marine mysid "Leptomysis lingvura": effects of age and starvation

Máster Universitario en Oceanografía

GDH activity and ammonium excretion during growth in the marine mysid, "Leptomysis lingvura"

[EN] Ammonium (NH4+) and nitrate (NO3-) are the main constituents of the inorganic nitrogen pool that supports primary production in marine systems. NH4+ release via glutamate deamination in heterotrophic organisms represents the largest recycled nitrogen source in the euphotic zone, supporting around the 80 % of the primary producers requirements (Harrison, 1992). Glutamate dehydrogenase (GDH) is the enzyme that catalyzes this process. This fact has lead to the use of GDH activity as an index, a proxy, for physiological NH4+ formation. The result is a measure of potential excretion that avoids incubation artefacts due to manipulation of the organisms. The relationship between GDH activity and NH4+ excretion in cultures of the marine mysid Leptomysis lingvura is analyzed here. With interspecific and environmental interferences minimized, the study shows that the relationship between GDH activity and NH4+ excretion in L. lingvura is similar to equivalent results measured on mixed assemblages of zooplankton.

The impact of body size and starvation on the biochemistry and the physiology of ammonium excretion in the marine mysid, "Leptomysis lingvura"

[EN] Ammonium (NH4+) release by bacterial remineralization and heterotrophic grazers determines the regenerated fraction of phytoplankton productivity, so the measurement of NH4+ excretion in marine organisms is necessary to characterize both the magnitude and the efficiency of the nitrogen cycle. Glutamate dehydrogenase (GDH) is largely responsible for NH4+ formation in crustaceans and consequently should be useful in estimating NH4+ excretion by marine zooplankton.
Here, we address body size and starvation as sources of variability on the GDH to NH4+ excretion ratio (GDH/RNH4+). We found a strong correlation between the RNH4+ and the GDH activity (r2 = 0.87, n = 41) during growth. Since GDH activity maintained a linear relation (b = 0.93) and RNH4+ scaled exponentially (b =0.55) in well fed mysids, the GDH/RNH4+ ratio increased with size. However, the magnitude of its variation increased even more when adult mysids were starved. In this case, the GDH/RNH4+ ratio ranged from 11.23 to 102.41.

Is the GDH/RNH4+ ratio in the mesozooplankton constant through different oceanic systems?

[EN] Nitrogen (N) is essential for life, but its availability is frequently limited in ocean ecosystems. Among all the compounds which influence the N pool, ammonium (NH4+) represents the major source of N for autotrophs. This NH4+ is provided by bacterial remineralization and heterotrophic grazers, with the mesozooplankton responsible for 12% to 33% of the total NH4+ recycled.  Quantifying the excretion physiology of zooplankton is then, necessary to understand the basis of an aquatic ecosystem’s productivity.
The measurement of glutamate dehydrogenase (GDH) activity has been widely used to assess the NH4+ excretion rates in planktonic communities. However, its relationship with the physiology varies with temperature and the nutritional status of the organisms, among other variables. Here we compare the GDH/RNH4+ ratio between oceanic regions with different trophic conditions.  Strengthening our knowledge of the relationship between GDH activities and the NH4+ excretion rates will lead to more meaningful interpretations of the mesoscale variations in planktonic NH4+ excretion.

Is the GDH/RNH4+ ratio in the mesozooplankton constant through different oceanic systems?

[EN]Nitrogen (N) is essential for life, but its availability is frequently limited in ocean ecosystems. Among all the compounds which influence the N pool, ammonium (NH4+) represents the major source of N for autotrophs. This NH4+ is provided by bacterial remineralization and heterotrophic grazers, with the mesozooplankton responsible for 12% to 33% of the total NH4+ recycled. Quantifying the excretion physiology of zooplankton is then, necessary to understand the basis of an aquatic ecosystem?s productivity. The measurement of glutamate dehydrogenase (GDH) activity has been widely used to assess the NH4+ excretion rates in planktonic communities. However, its relationship with the physiology varies with temperature and the nutritional status of the organisms, among other variables. Here we compare the GDH/RNH4+ ratio between oceanic regions with different trophic conditions. Strengthening our knowledge of the relationship between GDH activities and the NH4+ excretion rates will lead to more meaningful interpretations of the mesoscale variations in planktonic NH4+ excretion.