Inverse Metabolic Engineering of Synechocystis PCC 6803 for Improved Growth Rate and Poly-3-hydroxybutyrate Production

Synechocystis PCC 6803 is a photosynthetic bacterium that has the potential to make bioproducts from carbon dioxide and light. Biochemical production from photosynthetic organisms is attractive because it replaces the typical bioprocessing steps of crop growth, milling, and fermentation, with a one-step photosynthetic process. However, low yields and slow growth rates limit the economic potential of such endeavors. Rational metabolic engineering methods are hindered by limited cellular knowledge and inadequate models of Synechocystis. Instead, inverse metabolic engineering, a scheme based on combinatorial gene searches which does not require detailed cellular models, but can exploit sequence data and existing molecular biological techniques, was used to find genes that (1) improve the production of the biopolymer poly-3-hydroxybutyrate (PHB) and (2) increase the growth rate. A fluorescence activated cell sorting assay was developed to screen for high PHB producing clones. Separately, serial sub-culturing was used to select clones that improve growth rate. Novel gene knock-outs were identified that increase PHB production and others that increase the specific growth rate. These improvements make this system more attractive for industrial use and demonstrate the power of inverse metabolic engineering to identify novel phenotype-associated genes in poorly understood systems.

Decision making and planning of children in the reproductive process of males

Reproduction is not restricted to the biological; it is also a complex and dynamic social process. The objective of this paper is to analyze the decision and planning of children as part of the reproductive process in men. Methodologically, a qualitative research was made. The analysis includes in-depth interviews with 30 parents between 20 and 45 years, of medium socio-economic level in México. The results show that the reproductive process incorporates family formation negotiated with their couple the marriage until they had economic potential. In some cases, their decision hastened because the couple was pregnant. The planning was not contemplated but when getting the news, they accept it and get involved in the process, while others see it as a conflict.

El rol de la cooperación energética en la política exterior China: caso Venezuela (2001-2014)

Las reformas económicas iniciadas por Deng Xiaoping en China y la continuación de estas por sus líderes, han hecho del país asiático una de las mayores fuerzas económicas en el mundo. Sin embargo, su crecimiento económico se ve obstaculizado cuando sus fuentes de recursos energéticos escasean. A partir de esto, China ha iniciado una estrategia en política exterior que combina herramientas de baja política, como lo son la economía y la atracción cultural, para obtener un mayor acceso a hidrocarburos. Uno de los escenarios en donde dicha estrategia se ha hecho más visible es en la relación establecida con Venezuela. En este trabajo se discuten los principales intereses que China tiene en la región, la relación de cooperación energética que entrañan los dos países y las estrategias de corte geoeconómico que la potencia emergente ha implementado para garantizar un aumento en su potencial económico.

Economia política da corrupção : caso dos Estados Lusófonos

Elaborar uma Economia Política da Corrupção, como falha do Estado, é reconhecer que o Estado serve elites e é necessária legislação concreta para limitar a actuação e os excessos da classe política e dos funcionários da Administração. A questão central para tese é a da verificação da existência de uma relação entre a pobreza e o nível de corrupção nos Estados Lusófonos, medida pela Transparency International. Depois de avaliado o estado da arte e feita a história do próprio estudo da corrupção, utilizando os métodos da análise económica e do estudo de opinião, concluímos que as críticas ao Índice de Percepção da Corrupção elaborado pela Transparency International não colhem. Com efeito, a tese prova que a corrupção diminui o potencial de uma economia. Usando métodos econométricos concluimos ainda pela existência de uma relação linear entre o Produto Interno Bruto per capita e o rating dos Estados Lusófonos no Índice de Percepção da Corrupção. Finalmente, um estudo de opinião sobre “a corrupção em Portugal”, elaborado em 2008, conclui que é nos Serviços de Saúde e no Fisco que há mais corrupção em Portugal, um país, contudo, que fez o upgrading legislativo nos últimos anos e onde a corrupção, apesar de tudo, não atinge os níveis de outros Países de Expressão Oficial Portuguesa.

Identifying and understanding factors influencing the uptake of new technologies on dairy farms in SW England using the theory of reasoned action

Whilst much is known of new technology adopters, little research has addressed the role of their attitudes in adoption decisions; particularly, for technologies with evident economic potential that have not been taken up by farmers. This paper presents recent research that has used a new approach which examines the role that adopters' attitudes play in identifying the drivers of and barriers to adoption. The study was concerned with technologies for livestock farming systems in SW England, specifically oestrus detection, nitrogen supply management, and, inclusion of white clover. The adoption behaviour is analysed using the social-psychology theory of reasoned action to identify factors that affect the adoption of technologies, which are confirmed using principal components analysis. The results presented here relate to the specific adoption behaviour regarding the Milk Development Council's recommended observation times for heat detection. The factors that affect the adoption of this technology are: cost effectiveness, improved detection and conception rates as the main drivers, whilst the threat to demean the personal knowledge and skills of a farmer in 'knowing' their cows is a barrier. This research shows clearly that promotion of a technology and transfer of knowledge for a farming system need to take account of the beliefs and attitudes of potential adopters. (C) 2006 Elsevier Ltd. All rights reserved.

Small business collaboration through electronic marketplace

It is widely recognized that small businesses with less than 50 employees make significant contributions to the prosperity of local, regional, and national economies. They are a major source of job creation and a driving force of economic growth for developed countries like the USA (Headd, 2005; SBA, 2005), the UK (Dixon, Thompson, & McAllister, 2002; SBS, 2005), Europe (European Commission, 2003), and developing countries such as China (Bo, 2005). The economic potential is further strengthened when firms collaborate with each other; for example, formation of a supply chain, strategic alliances, or sharing of information and resources (Horvath, 2001; O’Donnell, Cilmore, Cummins, & Carson, 2001; MacGregor, 2004; Todeva & Knoke, 2005). Owing to heterogeneous aspects of small businesses, such as firm size and business sector, a single e-business solution is unlikely to be suitable for all firms (Dixon et al., 2002; Taylor & Murphy, 2004a); however, collaboration requires individual firms to adopt standardized, simplified solutions based on open architectures and data design (Horvath, 2001). The purpose of this article is to propose a conceptual e-business framework and a generic e-catalogue, which enables small businesses to collaborate through the creation of an e-marketplace. To assist with the task, analysis of data from 6,000 small businesses situated within a locality of Greater Manchester, England within the context of an e-business portal is incorporated within this study.

The economics of enhanced landfill mining: private and societal performance drivers

This paper addresses the economics of Enhanced Landfill Mining (ELFM) both from a private point of view as well as from a society perspective. The private potential is assessed using a case study for which an investment model is developed to identify the impact of a broad range of parameters on the profitability of ELFM. We found that especially variations in Waste-to-Energy (WtE efficiency, electricity price, CO2-price, WtE investment and operational costs) and ELFM support explain the variation in economic profitability measured by the Internal Rate of Return. To overcome site-specific parameters we also evaluated the regional ELFM potential for the densely populated and industrial region of Flanders (north of Belgium). The total number of potential ELFM sites was estimated using a 5-step procedure and a simulation tool was developed to trade-off private costs and benefits. The analysis shows that there is a substantial economic potential for ELFM projects on the wider regional level. Furthermore, this paper also reviews the costs and benefits from a broader perspective. The carbon footprint of the case study was mapped in order to assess the project’s net impact in terms of greenhouse gas emissions. Also the impacts of nature restoration, soil remediation, resource scarcity and reduced import dependence were valued so that they can be used in future social cost-benefit analysis. Given the complex trade-off between economic, social and environmental issues of ELFM projects, we conclude that further refinement of the methodological framework and the development of the integrated decision tools supporting private and public actors, are necessary.

Simple Question, Complex Answer : Pathways Towards a 50% Decrease in Building Energy Use

Addressing building energy use is a pressing issue for building sector decision makers across Europe. In Sweden, some regions have adopted a target of reducing energy use in buildings by 50% until 2050. However, building codes currently do not support as ambitious objectives as these, and novel approaches to addressing energy use in buildings from a regional perspective are called for. The purpose of this licentiate thesis was to provide a deeper understanding of most relevant issues with regard to energy use in buildings from a broad perspective and to suggest pathways towards reaching the long-term savings objective. Current trends in building sector structure and energy use point to detached houses constructed before 1981 playing a key role in the energy transition, especially in the rural areas of Sweden. In the Swedish county of Dalarna, which was used as a study area in this thesis, these houses account for almost 70% of the residential heating demand. Building energy simulations of eight sample houses from county show that there is considerable techno-economic potential for energy savings in these houses, but not quite enough to reach the 50% savings objective. Two case studies from rural Sweden show that savings well beyond 50% are achievable, both when access to capital and use of high technology are granted and when they are not. However, on a broader scale both direct and indirect rebound effects will have to be expected, which calls for more refined approaches to energy savings. Furthermore, research has shown that the techno-economic potential is in fact never realised, not even in the most well-designed intervention programmes, due to the inherent complexity of human behaviour with respect to energy use. This is not taken account of in neither current nor previous Swedish energy use legislation. Therefore an approach that considers the technical prerequisites, economic aspects and the perspective of the many home owners, based on Community-Based Social Marketing methodology, is suggested as a way forward towards reaching the energy savings target.

Mapeamento cromossômico de genes ribossomais em espécies estuarinas da família Gerreidae Identificação de uniformidade cariotípica e sua empregabilidade com fins biotecnológicos - REPROGEN

Perciformes are dominant in the marine environment, characterized as the largest and most diverse fish group. Some families, as Gerreidae, popularly known as silver jennies, carapebas, or mojarras have a high economic potential to marine fish farming, natural explotation and game fishing. Genetic information of these species are of fundamental importance for their management and production. Despite exist over 13,000 marine fish species described, only 2% were cytogenetically analyzed and less than 1% have some reproductive characteristics known. Induced breeding, cytogenetic characterization and cryopreservation of gametes, represent important areas in applied fish studies. In this project cytogenetic analyzes were performed to acess genetic aspects of Gerreidae species, distributed in coastal and estuarine regions of Northeast Brazil. Different methods for identifying chromosomal regions were employed using conventional techniques (Ag-NORs, C-banding), staining with base-specific fluorochromes (DAPI-CMA3), and physical mapping of ribosomal genes 18S and 5S rDNA, through hybridization in situ with fluorescent probes (FISH). The six species analyzed showed remarkable chromosome conservatism. The 18S and 5S ribosomal genes when analyzed in phylogenetic perspective demonstrate varied evolutionary dynamics, suggesting ocurrence of stasis process in some groups and greater dynamism in others. Double FISH with 18S and 5S probes showed both how efficient cytotaxonomic markers in the homogeneous karyotypes of this group of species. The karyotypic pattern identified in addition to the evolutionary aspects of karyotype, are suggestive of existence of low potential of post-zygotic barrier, prompting further research to prospect for artificial interspecific hybridization of these species of commercial importance

Aproveitamento da fibra do epicarpo do coco babaçu em compósito com matriz epóxi: estudo do efeito do tratamento da fibra

Nowadays the environmental issues are increasingly highlighted since the future of humanity is dependent on the actions taken by man. Major efforts are being expended in pursuit of knowledge and alternatives to promote sustainable development without compromising the environment. In recent years there has been a marked growth in the development of reinforced composite fiber plants, as an alternative for economic and ecological effects, especially in the substitution of synthetic materials such as reinforcement material in composites. In this current study the chemical- physical or (thermophysics )characteristics of the babassu coconut fiber, derived from the epicarp of the fruit (Orbignyda Phalerata), which the main constituents of the fiber: Klason lignin, insoluble, cellulose, holocellulose, hemicellulose and the content of ash and moisture will be determined. A study was conducted about the superficial modification of the fibers of the epicarp babassu coconut under the influence of chemical treatment by alkalinization, in an aqueous solution of NaOH to 2.5% (m/v) and to 5.0% to improve the compatibility matrix / reinforcement composite with epoxy matrix. The results of the changes occurred in staple fibers through the use of the techniques of thermogravimetric analyses (TG) and differential scanning calorimetry (DSC). The results found on thermal analysis on samples of fiber without chemical treatment (alkalinities), and on fiber samples treated by alkalinization show that the proposed chemical treatment increases the thermal stability of the fibers and provides a growth of the surface of area fibers, parameters that enhance adhesion fiber / composite. The findings were evaluated and compared with published results from other vegetable fibers, showing that the use of babassu coconut fibers has technical and economic potential for its use as reinforcement in composites

Rhamnolipid produced from agroindustrial wastes enhances hydrocarbon biodegradation in contaminated soil

A crude biosurfactant solution was produced by Pseudomonas aeruginosa growing on agroindustrial wastes as the substrate and used to study its effect on hydrocarbon biodegradation by the indigenous soil microflora under laboratory conditions. Two concentrations were studied at first and 1 mg of biosurfactant/g of soil showed to be the most efficient for the total petroleum hydrocarbon reduction, which reached 85% at the first 20 days in soil microcosms. Respirometric and microbial analyses showed that the biosurfactant added did not have toxic effects over the microbial population. The use of a biosurfactant for bioremediation has been limited because of its high cost production. Biosurfactants produced from cost-free by-products combines waste minimization with economic potential bioremediation process.

Profundidades de semeadura para emergência de plântulas de juazeiro

Juazeiro (Zizyphus joazeiro Mart.) é uma árvore brasileira típica dos sertões nordestinos. Endêmica da Caatinga, ela ocorre nos diversos Estados do Nordeste do Brasil e apresenta grande potencial econômico e é importante para a região semi-árida. Assim, este estudo teve como objetivo determinar a melhor profundidade de semeadura para a emergência de plântulas de Zizyphus joazeiro. O experimento foi realizado em casa-de-vegetação, tendo como substrato areia lavada e esterilizada contida em bandejas de plástico, com 4 repetições de 25 unidades de dispersão cada uma, nas seguintes profundidades (um, dois, três, quatro e cinco centímetros). A semeadura de juazeiro em ambiente protegido deve ser feita na profundidade entre 1,0 e 1,6cm, resultando em elevado aproveitamento das unidades de dispersão. A profundidade de 1cm resultou em menor tempo para a germinação total.

High levels of genetic differentiation and selfing in the Brazilian cerrado fruit tree Dipteryx alata Vog. (Fabaceae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Paraná Basin: Mineral resource potentials in Brazil, Uruguay, and Paraguay

The Paraná Basin is a Western Gondwanan cratonic basin that is better defined as the Paraná depositional site, since it has a diverse history as a basin. Sedimentation started in the Ordovician-Silurian, followed by extensive marine Devonian deposition. A Late Paleozoic/ Triassic facies cycle wedge was clearly built during Pangean time. The Early Cretaceous was characterized by extensive basaltic lava flows immediately before the break-up of Pangea. Following these rifting and drifting processes, the basin's structural framework was totally rebuilt, generating new depositional sites in the Late Cretaceous to Tertiary. Based on more recent data, at least two different basins may be defined during the evolution of what was once considered a unique basin. Nevertheless, even if considered as a single basin, the sedimentary pile of the Paraná Basin has considerable economic potential, until now exploited only rudimentarily, except for its groundwater resources. Aggregates, limestones, clays, industrial sands, gems, dimension stones, hydrocarbons, coal, peat, and uranium are some of the potential mineral resources of this basin. Copyright © 1997 by V. H. Winston & Son, Inc. All rights reserved.

Rochas ornamentais e de revestimento do sudoeste do estado do mato grosso

Geological, petrographical, chemicals and technological studies of rocks of the southwest region of Mato Grosso State, Brazil have the purpose of qualification and application of these rocks as dimension stones. The first objective is to enlarge the offering of new esthetic varieties that show a standardization of technologic patterns, adequate characterization and classification assuring the correct specification of the rocks in relation to the use as dimension stones. Ten rock types are recognized for dimension stones, including granites, diabases, gneisses and amphibolites grouped by its colors in red, grey and black types and showing economic potential of plowing. The results obtained in the technological essays show that the rock parameters are sited inside the standard limits established by technical rules and obey satisfactorily the limit values fixed for granites in internal and external covering uses.