Application of micronucleus test and comet assay to evaluate BTEX biodegradation

The BTEX (benzene, toluene, ethylbenzene and xylene) mixture is an environmental pollutant that has a high potential to contaminate water resources, especially groundwater. The bioremediation process by microorganisms has often been used as a tool for removing BTEX from contaminated sites. The application of biological assays is useful in evaluating the efficiency of bioremediation processes, besides identifying the toxicity of the original contaminants. It also allows identifying the effects of possible metabolites formed during the biodegradation process on test organisms. In this study, we evaluated the genotoxic and mutagenic potential of five different BTEX concentrations in rat hepatoma tissue culture (HTC) cells, using comet and micronucleus assays, before and after biodegradation. A mutagenic effect was observed for the highest concentration tested and for its respective non-biodegraded concentration. Genotoxicity was significant for all non-biodegraded concentrations and not significant for the biodegraded ones. According to our results, we can state that BTEX is mutagenic at concentrations close to its water solubility, and genotoxic even at lower concentrations, differing from some described results reported for the mixture components, when tested individually. Our results suggest a synergistic effect for the mixture and that the biodegradation process is a safe and efficient methodology to be applied at BTEX-contaminated sites. © 2012 Elsevier Ltd.

Avaliação dos efeitos citotóxicos, genotóxicos e mutagênicos do BTEX, antes e após o processo de biorremediação por microrganismos, utilizando os sistemas teste de Allium cepa e cultura de células de mamífero

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Efeito do uso de enzimas proteolíticas na maturação de queijo Prato com teor reduzido de gordura

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise funcional e estrutural comparativa da fastuosaina com papaína e bromelinas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da viabilidade do lodo de esgosto como recondicionante de solos agrícolas, após processo de atenuação natural, por meio de diferentes bioensaios

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Queijo Prato com teor reduzido de gordura adicionado de enzima proteolítica: características físicas e sensoriais

The Prato cheese is the second most consumed cheese in Brazil. It is produced by milk enzymatic coagulation, and maturated for at least 25 days; it is classified as fatty cheese and of medium moisture. Due to the concern to health, the cheeses consumers have been seeking for products with low fat contents; however fat is essential for providing desirable sensory and physiologic characteristics, such as flavor, softness and texture to cheeses. Alterations on the technological processing of low fat cheeses have been made seeking for improved products, and the use of proteolytic enzymes has been a significant strategy. The meltability, color and sensory characteristics are fundamental quality indicators of the final products. This study reports the findings from the analyses on the physical and sensory characteristics of low fat Prato cheese with addition of proteolytic enzyme – fastuosain, that is extracted from unripe gravata fruit. The addition of fastuosain improved the quality of the product, as this additive neither affected the meltability, nor produced bitterness, which is a common unpleasant taste in low fat cheeses.

Escoamento da produção de café: uma projeção de logística

This paper seeks to show which impacts there are of the combined use of different modal for the coffee’s outflow. Nowadays is used, almost exclusively, the roadway to transport. How-ever, for this product with low added value, the types of transports best suited, in the litera-ture, are the railway and waterway. For this, is describe the development and spread of coffee throughout Brazil. Subsequently, is calculate the current cost of transporting two producing regions (Minas Gerais and Rondônia), using for that information about the freight, the social cost, and the level of losses along the way. After that is projected costs if utilizes the multi-modal model, when considering the same cost parameters used for the current model. Here-after, an analysis is made of the obtained cost, speed, consistency, capacity, availability and frequency of each transport model. Therefore, note that the multi-modal system is more ef-ficient and economical than current, based on transport by road

Historia de la ciudad de Denia

V.1 (XVI, 290, 5 p., 8 h. de lám., 1 fot. pleg.) -- v.2 (319 p.)

Memoria para escribir la biografía de Don Rafael Esteve, Primer Grabador de Cámara de S.M.

Mención de responsabilidad tomada del texto introductorio

Diana desenterrada : antiguas memorias y breve recopilacion de los mas notables sucessos de la ciudad de Denia, y su famoso templo de Diana : desde su antiquisima fundacion hasta el estado presente

Explicit : "... / y a su pesar desentierra / de Diana la memoria."

Noticia para Denia

Contiene : cop

Fiestas de Denia al Rey catholico Felipo III de este nombre...

Colofón

Breve y curioso romance en que se refieren las calidades y circunstancias de un pescado que el dia 2 de abril de este año 1761 saliò en la playa... que llaman el Molinell

Fecha tomada del tít

Ataque de Valencia [Material gráfico] : Valencia derrota delante de sus murallas al Mariscal Moncey, y le pone en vergonzosa fuga

Resumen: Descripción: vista posterior de las Torres de Quart con cañones y gente luchando

Origens del grabat en Valencia : apunts critich-histórichs

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