Early ontogeny of Ancherythroculter nigrocauda and effects of delayed first feeding on larvae

Development of embryos and larvae in Ancherythroculter nigrocauda Yih et Woo (1964) and effects of delayed first feeding on larvae were observed after artificial fertilization. The fertilized eggs were incubated at an average temperature of 26.5 degrees C (range: 25.7-27) and the larvae reared at temperatures ranging from 21.8 to 28 degrees C. First cleavage was at 50 min, epiboly began at 7 h 5 min, heartbeat reached 72 per min at 24 h 40 min and hatching occurred at 43 h 15 min after insemination. Mean total length of newly hatched larvae was 4.04 +/- 0.03 mm (n = 15). A one-chambered gas bladder was observed at 70 h 50 min, two chambers occurred at 15 days, and scales appeared approximately 30 days after hatching. Larvae began to feed exogenously at day 4 post-hatch at an average temperature of 24 degrees C. Food deprivation resulted in a progressive atrophy of skeletal muscle fibres, deterioration of the larval digestive system and cessation of organ differentiation. Larval growth under food deprivation was significantly affected by the time of first exogenous feeding. Starved larvae began to shrink, with negative growth from day 6 post-hatch. The point of no return (PNR) was reached at day 11 after hatching. Mortality of starved larvae increased sharply from day 12 after hatching.

Effects of live food and formulated diets on survival, growth and protein content of first-feeding larvae of Plelteobagrus fulvidraco

In recent years, much progress has been made in the rearing of fish larvae fed only artificial diets. A preliminary study was made in an attempt to evaluate the effects of live food and formulated diets on survival, growth and body protein content of first-feeding larvae of Plelteobagrus fulvidraco. Three test diets varying in protein level were formulated: Feed 1 containing 45% protein, Feed 2 with 50% protein and Feed 3 with 55% protein. Larvae fed live food (newly hatched Artemia, unenriched) were the control. The experiment started 3 days post-hatch and lasted for 23 days. At the end of the 23-day trial, survival was best in the control group (65.6%) whereby the final body weight and specific growth rate (SGR) were significantly lower than those in the test feed groups. At the same time, coefficients of variation for SGR and final body weight in the test groups were significantly higher than those in the control. Whole body protein content in all treatments showed a similar tendency during development: significantly higher 3 days post-hatch, then decreasing significantly, and then increasing unstatistically 10 days post-hatch. All results suggest that live food is still better for first-feeding larvae of P. fulvidraco, since live food leads to healthier larvae growth.

The role of sense organs in the feeding behaviour of Chinese perch

Experiments were conducted to identify the rules of the individual sense organs in the feeding behaviour of Chinese perch Siniperca chuatsi by determining the consumption of natural food after selective removal or blocking of eyes, lateral lines and olfactory organs, and also by observing the behavioural response to visual, mechanical and chemical stimulation by artificial prey. Chinese perch were able to feed properly on live prey fish when either eyes or lateral lines were intact or functional, but could scarcely feed without these two senses. Chinese perch recognized its prey by vision through the perception of motion and shape, and showed a greater dependence on vision in predation when both visual and mechanical cues were available. Chemical stimulation by natural food could not elicit any feeding response in Chinese perch, and gustation was only important to the fish for the last stage of food discrimination in the oropharyngeal cavity. The sensory basis of Chinese perch in feeding is well adapted to its nocturnal stalking hunting strategy. and also explains its peculiar food habit of accepting live prey fish only and refusing dead prey fish or artificial diets. (C) 1998 The Fisheries Society of the British Isles.

Impacto da iluminação natural e artificial na disponibilidade de macro-invertebrados bentónicos para as aves limícolas

Tese de mestrado em Ecologia Marinha, apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2015

Feeding substrates to black coconut bunch weevil, Homalinotus coriaceus (Gyllenhal) (Coleoptera: Curculionidae), rearing in laboratory

A broca do cacho do coqueiro, Homalinotus coriaceus (Gyllenhal), é uma praga limitante da produção de coco no Brasil, sendo que tanto as larvas como os adultos provocam a queda das flores femininas e dos frutos imaturos, pela interceptação do fluxo de seiva ou pela alimentação direta nas estruturas reprodutivas. em virtude da escassez de informações sobre sua biologia, realizou-se esse trabalho com o objetivo de desenvolver uma metodologia mais adequada para a criação da praga em laboratório. Foram utilizados os parâmetros biológicos para avaliação e comparação dos sistemas de criação estudados.Toletes de cana-de-açúcar foram utilizados como substrato para alimentação dos adultos coletados no campo e obtenção dos ovos. As larvas foram criadas em três substratos alimentares no Laboratório de Entomologia da Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-CPATC (Centro de Pesquisa Agropecuária dos Tabuleiros Costeiros), em Aracaju, SE. Os substratos alimentares estudados foram: o mesocarpo do coco, dieta para criação da broca dos citros e dieta para criação da broca do olho do coqueiro, sendo esta a que proporcionou o melhor desenvolvimento larval num menor tempo, com boa viabilidade, maior facilidade no preparo e manutenção.

Influência de fotoperíodo artificial no comportamento de um primata neotropical diurno (Callithrix jacchus)

One of the main environmental cues for the adjustment of temporal organization of the animals is the light-dark cycle (LD), which undergoes changes in phase duration throughout the seasons. Photoperiod signaling by melatonin in mammals allows behavioral changes along the year, as in the activity-rest cycle, in mood states and in cognitive performance. The aim of this study was to investigate if common marmoset (Callithrix jacchus) exhibits behavioral changes under short and long photoperiods in a 24h cycle, assessing their individual behaviors, vocal repertoire, exploratory activity (EA), recognition memory (RM) and the circadian rhythm of locomotor activity (CRA). Eight adult marmosets were exposed to a light-dark cycle of 12:12; LD 08:16; LD 12:12 and LD 16:08, sequentially, for four weeks in each condition. Locomotor activity was recorded 24h/day by passive infrared motion detectors above the individual cages. A video camera system was programmed to record each animal, twice a week, on the first two light hours. From the videos, frequency of behaviors was registered as anxiety-like, grooming, alert, hanging position, staying in nest box and feeding using continuous focal animal sampling method. Simultaneously, the calls emitted in the experimental room were recorded by a single microphone centrally located and categorized as affiliative (whirr, chirp), contact (phee), long distance (loud shrill), agonistic (twitter) and alarm (tsik, seep, see). EA was assessed on the third hour after lights onset on the last week of each condition. In a first session, marmosets were exposed to one unfamiliar object during 15 min and 24h later, on the second session, a novel object was added to evaluate RM. Results showed that long days caused a decreased of amplitude and period variance of the CRA, but not short days. Short days decreased the total daily activity and active phase duration. On long days, active phase duration increased due to an advance of activity onset in relation to symmetric days. However, not all subjects started the activity earlier on long days. The activity offset was similar to symmetric days for the majority of marmosets. Results of EA showed that RM was not affected by short or long days, and that the marmosets exhibited a decreased in duration of EA on long days. Frequency and type of calls and frequency of anxiety-like behaviors, staying in nest box and grooming were lower on the first two light hours on long days. Considering the whole active phase of marmosets as we elucidate the results of vocalizations and behaviors, it is possible that these changes in the first two light hours are due to the shifting of temporal distribution of marmoset activities, since some animals did not advance the activity onset on long days. Consequently, the marmosets mean decreased because the sampling was not possible. In conclusion, marmosets synchronized the CRA to the tested photoperiods and as the phase angle varied a lot among marmosets it is suggested that they can use different strategies. Also, long days had an effect on activity-rest cycle and exploratory behaviors

Reproduction of Cichla kelberi Kullander and Ferreira, 2006 introduced into an artificial lake in southeastern Brazil

O objetivo do estudo foi caracterizar a reprodução de Cichla kelberi em um lago artificial no município de Leme, Estado de São Paulo, Brasil. A época reprodutiva ocorreu na primavera e verão. O L50 e o L100 desta espécie foram de 192 e 235 mm (L50) para machos e fêmeas, respectivamente e 290 mm (L100) para ambos os sexos. A desova foi do tipo parcelado. O tamanho dos ovócitos maduros foi de 428,4 µm, atingindo o máximo em 2.203,2 µm. A média da fecundidade foi de 12.129,2 ovócitos com média de cada desova de 4.897,7 ovócitos. Esta espécie adequou-se à definição de peixes com fecundação externa, não migradoras e com cuidado parental da prole. Os atributos para a introdução dos tucunarés podem ser tentadores. Porém, suas características reprodutivas e alimentares fazem com que não existam competidores ou predadores naturais e, assim, a sua expansão populacional seja de difícil controle e erradicação.

Seasonal and ontogenetic variations in the diet of Cichla kelberi Kullander and Ferreira, 2006 introduced in an artificial lake in southeastern Brazil

A dieta de Cichla kelberi introduzido em um lago artificial em Leme-SP foi composta predominantemente pelas espécies de peixes mais comuns nesse lago (Oreochromis niloticus e o próprio C. kelberi). Na primavera e no verão, o item mais consumido foi O. niloticus. Porém, o canibalismo foi muito comum para esta espécie. As altas frequências de O. niloticus e de C. kelberi revelam que a espécie apresenta um ciclo sazonal, se alimentando das presas mais comuns em cada período do ano, com uma redução da sua atividade alimentar durante o inverno. As dietas foram diferentes entre os exemplares imaturos e maduros, sugerindo que existem diferenças ontogenéticas, principalmente relacionadas ao tipo de presa, como: Ephemeroptera, consumidos pelos tucunarés imaturos e peixes, pelos maduros, além do tamanho das presas.

Co-feeding of pacu, Piaractus mesopotamicus Holmberg (1887), larvae with Artemia naupili and a microencapsulated diet

Aiming at a precocious substitution of live prey by artificial diet, a 20-day experiment with pacu, Piaractus mesopotamicus, larvae using co-feeding and abrupt weaning strategies was set up. At the end of the experiment, larvae fed Artemia showed the best results (P < 0.05) in weight, total length and biomass, compared with other treatments. Larvae fed exclusively a microencapsulated diet never ingested the diet. Diet ingestion in co-fed and abrupt-weaned larvae was low, but did increase during the experiment; however, Artemia influenced diet ingestion on co-fed larvae. Careful considerations should be given to diet processing and formulation to ensure survival and growth of larvae fed exclusively on prepared diets. © 2005 by The Haworth Press, Inc. All rights reserved.

Relação peso-comprimento e fator de condição de Cichla kelberi (Perciformes, Cichlidae) introduzidos em um lago artificial no sudeste Brasileiro

The length-weight relationship and condition factor for Cichla kelberi introduced in an artificial lake in southeastern Brazil were analyzed. By studying separately the male, female and immature individuals, different length-weight patterns were found to happen among them. The same happened when distinct seasonal variations were compared. They are related to feeding alterations and me reproductive periods. The length-weight relationship and condition factor for these fishes vary in accordance with the time of introduction, population size, the characteristics of the ecosystem, sexual maturity, as well as the intra- and interspecific interactions.

Avaliação eletromiográfica dos músculos masseter, temporal e bucinador de lactentes em situação de aleitamento natural e artificial

Pós-graduação em Pediatria - FMB

Avaliação do efeito dos principais itens da dieta natural de Astronotus ocellatus (Cuvier, 1829) da Reserva Mamirauá (AM, Brasil) sobre a sua coloração reprodutiva em ambiente artificial

Com o objetivo de testar a influência dos itens alimentares na coloração de acara-açus foi realizado o presente estudo que teve duas etapas. A primeira visou identificar os principais grupos alimentares da dieta de Astronotus ocellatus através da análise dos conteúdos estomacais e intestinais. A segunda visou comparar o efeito ocasionado pela administração de diferentes grupos da dieta, num ambiente artificial, sobre a coloração vermelha e a aquisição de massa corpórea dos indivíduos. Na primeira etapa as atividades foram desenvolvidas na Reserva de Desenvolvimento Sustentável Mamirauá (RDSM. Foram utilizados 216 indivíduos. Após fixação do trato digestivo de cada exemplar estes foram analisados qualitativamente, sobestereomicroscopia. Os itens alimentares encontrados nos referidos conteúdos foram classificados usando como critério de agrupamento grandes categorias tais como: moluscos, crustáceos, insetos, peixes e vegetais, além de material não identificado. O comprimento da primeira maturação sexual foi calculado. O regime do nível de água na RDSM durante o período do estudo foi obtido através de dados climáticos fornecidos pelo Instituto Mamirauá. O índice alimentar para cada item, foi calculado através do produto da freqüência de ocorrência relativa e do peso relativo de cada item e da somatória dos produtos para todos os itens identificados, os principais itens identificados foram peixes, insetos e moluscos. Foram capturados 20 indivíduos de Astronotus ocellatus, desta vez na Ilha do Marajó-PA, no mês de fevereiro/2006. Para o recebimento dos animais capturados foram preparados quinze (15) aquários na estação de piscicultura do Utinga (Belém, PA), com renovação de água constante. Com base nos resultados obtidos na primeira etapa e com base na literatura, elaborou-se o delineamento experimental com cinco tratamentos alimentares: T₁ - Ração comercial (controle); T₂ – Músculo de peixe; T₃ – Moluscos, T₄ - Insetos; T₅ – Crustáceos. A análise do Índice de Intensidade de Coloração Vermelha foi baseada na metodologia de comparação computacional dos níveis de intensidade de cor proporcionada por software específico. Para efeito de comparação utilizou-se o Incremento da Coloração Vermelha do ocelo e da coloração lateral difusa. O tratamento realizado com a dieta de molusco apresentou o maior índice de intensidade de coloração vermelha no ocelo ao final de 20 dias. O tratamento realizado com a dieta de crustáceo gerou o maior índice de intensidade da coloração vermelha lateral difusa ao final de 20 dias. Os animais submetidos a quase todos os tratamentos apresentaram um aumento na massa corpórea ao longo de 40 dias de experimento, mas principalmente aqueles alimentados com moluscos demonstraram maior aquisição de biomassa.

On-plant larval movement and feeding behavior of fall armyworm (Lepidoptera: Noctuidae) on reproductive corn stages

Spodoptera frugiperda J.E. Smith (fall armyworm) is considered one of the most destructive pests of corn throughout the Americas. Although this pest has been extensively studied, little is known about its larval movement and feeding behavior on reproductive compared to vegetative corn stages. Thus, we conducted studies with two corn stages (R1 and R3) and four corn plant zones (tassel, above ear, ear zone, and below ear) in the field at Concord, NE (USA), and in the field and greenhouse at Botucatu, SP (Brazil), to investigate on-plant larval movement. The effects of different corn tissues (opened tassel, closed tassel, silk, kernel, and leaf), two feeding sequence scenarios (closed tassel-leaf-silk-kernel and leaf-silk-kernel), and artificial diet (positive control) on larval survival and development were also evaluated in the laboratory. Ear zone has a strong effect on feeding choice and survival of fall armyworm larvae regardless of reproductive corn stage. Feeding site choice is made by first-instar. Corn leaves of reproductive plants were not suitable for early instar development, but silk and kernel tissues had a positive effect on survival and development of fall armyworm larvae on reproductive stage corn.

Effects of artificial foods on the blood chemistry of the Australian magpie

Bird feeding on residential property is a popular activity throughout Western countries. Advocates insist the practice is beneficial, while opponents maintain that it can result in a wide range of negative outcomes including malnutrition. The biological effects of 'backyard feeding' were studied in Australian magpies Gymnorhina tibicen during the non-breeding season in 1999 in the Greater Brisbane and the Lockyer Valley regions, south-east Queensland, Australia. Six magpie populations were selected and 70 birds were individually tagged for identification. The birds were provided with processed foods, 20-40 g per bird daily. To monitor the effects of the food, blood chemistry and body mass (BM) were used as indices. Significant effects were observed in BM and plasma cholesterol (PC), showing strong sensitivity to food provisioning. Significant effects on PC and uric acid were found only when birds were fed dog sausage. Results suggest that blood PC levels in magpies are readily influenced by, probably, the lipids present in food, and that the type of food can affect blood PC levels. These effects may occur widely among fed magpies if the influence that we demonstrated at plasma level can be generalized. Following the free-ranging study, six magpies were captured and subjected to a 6-day captive experiment to determine whether the selected foods had the potential to alter the birds' blood chemistry. It was found that all of the foods, when provided ad libitum, influence at least two of the three blood parameters (PC and non-esterified fatty acids). Due to its popularity, wildlife feeding will continue. To make wildlife-feeding activities truly sustainable, there is a need for further studies. This study clearly demonstrated that the physiology of wild magpies can be affected by 'backyard feeding'.

Risk from cattle trampling to nests of an endangered passerine evaluated using artificial nest experiments and simulations

Grasslands are often grazed by cattle and many grassland birds nest on the ground, potentially exposing nests to trampling. We tested for trampling risk introduced by cattle to nests of endangered Florida Grasshopper Sparrows (Ammodramus savannarum floridanus) using experimentally paired grids of artificial nests (i.e., clay targets) similar in size to nests of Florida Grasshopper Sparrows and counted the number of clay targets that were broken in paired grazed and ungrazed enclosures. Clay targets in grazed grids were trampled 3.9% more often than their respective ungrazed grids, and measurements of cattle presence or density were correlated with the number of broken clay targets, suggesting that excluding cattle during breeding is an important management recommendation for the Florida Grasshopper Sparrow. Trampling rates within grazed enclosures were spatially homogeneous with respect to cattle infrastructure such as supplemental feeding troughs and fences, and forests and stocking density were poor predictors of trampling rates when excluding ungrazed grids. We used population viability analysis to compare quasi-extinction rates, intrinsic growth rates, and median abundance in grazed and ungrazed Florida Grasshopper Sparrow aggregations to further understand the biological significance of management aimed at reducing trampling rates during the breeding season. Simulations indicated that trampling from grazing increased quasi-extinction rates by 41% while reducing intrinsic growth rates by 0.048, and reducing median abundance by an average of 214 singing males after 50 years. Management should avoid grazing enclosures occupied by Florida Grasshopper Sparrows during the nesting season to minimize trampling rates. Our methods that combine trampling experiments with population viability analysis provide a framework for testing effects from trampling on other grassland ground-nesting birds, and can directly inform conservation and management of the Florida Grasshopper Sparrow.