Mutagênese induzida por flavonóides presentes do decocto das cascas da aroeira (Schinus terebinthifolius, Raddi)

The decoction of Brazilian pepper tree barks (Schinus terebinthifolius, Raddi), is used in medicine as wound healing and antiinflamatory. Once extracts from this plant are used for acceleration of scar s process, it is important to study their mutagenic and genotoxic potential. In previous works in our laboratory, it was observed mutagenicity caused by the decoction when in high concentrations. Among the chemical compounds of this plant that could be able to induce mutation, the flavonoids were the only group that was referred to have either an oxidant or antioxidant potential. The flavonoids were isolated, purified and quantified by adsorptive column chromatography under silica gel, bacterial and in vitro genotoxic tests were realized to determine if the flavonoids were the responsible agents for this mutagenicity found. The tests realized with plasmidial DNA were indicative that the flavonoids are probably genotoxic, due to the presence of correlation between increase of the flavonoid concentration and in plasmidial DNA double strand breakage visualized in agarose gel, as well as they were capable to generated abasic sites shown by the in vitro treatment with exonuclease III. The same tests with plasmidial DNA in the presence of copper [10 µM] and of a Tris-HCl pH 7.5 [10 µM] buffer were realized with the isolated flavonoids to determine if there would be or not participation of reactive oxygen species (ROS). The transformation of plasmidial DNA in different bacterial strains proficient and deficient in DNA repair enzymes in the presence or not of a Tris-HCl buffer, suggests that the enzymes that repair oxidative lesions are necessary to repair the lesions generated by the flavonoids and that ROS are generated and are necessary to promote the lesions. Bacterial tests with Escherichia coli strains of the CC collection (deficient or not for DNA repair enzymes), showed that the flavonoids are able to increase the frequency of mutations, mainly in strains mutated in repair enzymes (MutM, MutY-glicosylases and double mutant), suggesting that these agents are responsible for the enhancement in the mutation rate. In order to determine the mutation spectrum caused by the flavonoids of the Brazilian pepper tree stem bark, plasmidial DNA previously treated with the flavonoids were transformed in bacterial strains deficient and proficient in the DNA repair enzymes, followed by a blue-white selection with X-gal, DNA amplification by PCR and sequencing the positive mutant clones. Analysis of the mutants obtained from strains CC104, CC104mutM, CC104mutY, CC104mutMmutY, BW9101, BW9109 indicated a predominance of some mutations like G:C to C:G that can be correlated with the origin of 8-oxoG, due to oxidative lesions caused by the flavonoids. So it can concluded that the flavonoid isolated or in fractions enriched on them are genotoxic and mutagenic, and their mutations are predominantly oxidative, mediated by ROS, and the lesions are recognized by the BER system. In this way it is proposed that the flavonoids can act in two different ways to generate the DNA lesion: 1. in a Fenton-like reaction, when the flavonoid are in the presence of metal ions and that together with the water generate ROS that promotes the DNA lesions; 2. in another way the lesions can be generated by the formation of ROS due to the internal chemical structure of the flavonoid molecule due to the quantity and location of hydroxyl groups, and so producing the DNA lesions, those lesions can be directly (suggested by the in vitro experiments) or indirectly done (supported by the experiments using the CC bacterial strains)

Capital social e igreja católica :expressões e práticas no sertão do Seridó

It historizes and it analyzes the social capital in the area Seridó. The traditions associative seridoenses are reconstructed starting from the dimensions: economical, social, religious person and politics. In them it is possible to notice actions that form the social capital of the area. The country of Seridó present an associative tradition based on the mutual help, in the trust and reciprocity that she remount there are decades in your history. The relationship among the Catholic Church, that historically it is present in the area, and rural communities, through your community associations, it is the backdrop where you/they are the responsible associative elements for the tear of the regional social fabric: in him (the backdrop) he/she is the responsible social capital for the work of the rural community organizations. The Catholic Church, through your social action and the Program of Combat to the Rural Poverty, of Rio Grande do Norte is the league that sustains the actions collective seridoenses

Miguilins no sertão da cabaça azul: incandescência, infância e devaneios poéticos em Mutum

Cette thèse concerne dans une lecture poétique du roman Campo Geral, de Guimarães Rosa, du film Mutum - basé sur le roman mentionné et réalisé par Sandra Kogut, et de l investigation das les villes de Minas Gerais/MG impliqués dans la production du film. Le but de cette lecture est de communiquer l'expérience onirique faite à partir d'une oeuvre cinématographique, à partir d un récit littéraire et la rencontre avec cinq acteurs non-professionnels et de trois membres de l'équipe de Mutum. Pour ça, le rêve poétique, proposé par Gaston Bachelard, il est utilisé comme une ressource cognitive à l'expérience de la réalité de semi-imaginaire l'homme, a partir de l actionnement du double dans le processus de participation affective (MORIN, 1997). Le film choisi abord des impressions d un enfant qui vit avec ses parents, avec ses frères, avec sa grand-mère et avec sa chienne Rebeca, dans un place appelé Mutum. Sous la perspective de l être rêveur, qui medite sur les images de l enfance onirique dans le contexte des régions sèches de la campagne, des régions qui sont transformées et étendues à travers le rêve poétique, nous arrivons à l enfance méditative (BACHELARD, 2006). Tout au long de la narration de cette recherche, Guimarães Rosa, la réalisatrice Sandra Kogut, moi-même comme un individu / chercheur et les interlocuteurs de la famille Mutum que j'ai trouvé dans Minas Gerais, nous sommes tous pris comme Miguilins qui rêvent de La région Cabaça Azul. Incandescents, multiples, primitives, ces Miguilins sont porteurs des papiers colores et avec elle ils comprennent la participation de l'homme dans le Grand Récit (SERRES, 2005)

Capital Social e Desenvolvimento Rural: acesso, uso e gestão da água nos Territórios Rurais do Sertão do São Francisco

This study aimed to contribute to the discussion of social capital, seeking to relate that to the access, use and water resources management in the Sao Francisco Vale, specifically in rural areas of the wilderness of San Francisco situated in the State of Bahia and Pernambuco. As, stimulate action possibilities for individuals (family rural) apart by a patrimony public so precious that is water. Besides a theoretical discussion of social capital (networks, trust, participation) and rural development. We applied 387 questionnaires to farmers and some interviews with actors social of territorial forum and committee of the São Francisco basin where it was possible to correlate our variables in order to confirm our hypothesis: social capital is a key element to ensure access, use and management of water for rural families living in irrigated and rainfed areas

Os empoderados da agricultura familiar: capital social nos territórios do seridó e do sertão do Apodi/RN

The study of social practices aims to overcome the theoretical dichotomies that insist on separating the individual from the social structures and vice versa. In this sense, the debate between objectivism and subjectivism in the construction of social reality still has occupied much time and reflection of various scholars of the humanities. Pierre Bourdieu has extensive work that seeks to advance in relation to the theoretical framework of traditional sociological explanations. Bourdieu`s approach regarding social practices is considered by some researchers as a synthesis of classical theories and by others as an attempt of complexify contemporary studies on the significance of social life. This thesis sharesthis effort to understand the social practices of agents, aiming to analyze the strategies of social and political leaders of family farming in the territories of Seridó and Apodi in Rio Grande do Norte, and it has as reference the theoretical and methodological concepts of habitus, field and capital, in Bourdieu. Therefore, we studied the trajectories of social leaders, here called agents as family farming in the two locations in Rio Grande do Norte. As techniques and procedures of the study, we resortedto semi-structured interviews, observations, participation in events and other researches. In conclusion, this thesis gives an account of the construction of two different relational fields for the activities of agents of family farming in the territories of Seridó and Apodi. Although the relational fields in the territories have been structured under the same prevailing institutions, which are: church, union and political party, the social practice of agents shows itself from social position and political variety. Even with the similarities and differences identified and analyzed in different fields of construction, the social relations of the agents in the territories result in the construction of gated communities, the social capital that is the substrate which the agents called empowered .

Geração de renda e ocupações na cadeia agroindustrial de castanha de caju no Rio Grande do Norte: o caso do sertão de Apodi

This work is part of the research group studying agribusiness chains, by characterizing agroindustrial chain of cashew nuts in Rio Grande do Norte, with special emphasis on Sertão de Apodi Citizenship Territory, showing their different flows and linkages and analyzes generating jobs and income in the production of this good, from the experience of COOPAPI, using as a methodology for analyzing the input-output matrix. And this is justified by the fact that Brazil is in the context as the world's fifth largest producer of cashew nuts and the production of this product focus in the Northeast, especially the Rio Grande do Norte, which is the second largest producer of region, behind only the state of Ceará, which is the largest producer in the country. Moreover, in recent years the state has increased its insertion in international markets this product, particularly the Mossoró-Assu Territory, which constitutes the largest producer in the state, particularly the municipality of Serra do Mel, which has an arrangement different yield, based on the model of integrated rural development, embodied in family farming. Nevertheless, the Sertão de Apodi Citizenship appear as the fourth among the Citizenship territories of Rio Grande do Norte in the ranking of the production of cashew nuts, but the prevailing model of Cooperatives in the Territory, based on the Solidarity Economy, i,e, different from the old cooperative, which is based on the precepts as patronage, with cooperative owners, favor, etc.., encourages scientific research. In this, after a careful analysis of data from field research, it was noticed that among the links in the chain, the production link is what generates the most jobs, while the processing link commands to generate income, but a portion meaningful occupations and income are generated outside the territory, especially out of state RN, denouncing the gap in supply of some specific demands within the chain

Entre o mercado e a construção social: uma análise da experiência da implantação doPRONAF-infraestrutura e PRONAT nos territórios do Mato Grande-RN e sertão do Apodi-RN (1997 - 2010)

Analyzes the development experience in the Territories of Mato Grande and Sertão do Apodi in the state of Rio Grande do Norte, evaluating the actions of the National Program for Strengthening Family Agriculture, specifically the line of infrastructure (PRONAF-INFRA), and the National Program for Sustainable Development of Rural Territories (PRONAT) in these territories. Summarizes the various rural development approaches and takes the theoretical assumptions of territorial development, the concept of constructed territory and market-plan territory, further the cycle model to analyze public policies selected these experiences. Thus, we propose to test the hypothesis that most of the actions implemented would lead to the formation of market-plan territories, in other words, perceived only as a platform for the presentation of projects. The literature and documents, combined with case studies, interviews and direct observation of the meetings of committees, showed that, despite two boards are under the same laws, rules and formal regulations, have clear differences when considering the theory and concepts that were used as reference. The Apodi s territory is closer to a constructed space thus the search for a broader agenda, more autonomous and more appropriate to the reality experienced by local actors. On other hand the Territory of Mato Grande had the characteristics of a market-plan territory more present. As the result, the territory of Sertão do Apodi accesses not only as part of a greater number of policies and funding sources, ensuring a greater and more diverse investment volume than the territory of Mato Grande. Despite these differences, studies have shown that territorial boards surveyed are still far from becoming the main forum for managing the development from conception planning socially constructed. Showed, finally, that territorial development strategy is relevant, but requires a long walk and a deep and continuous learning process to be successfully implemented in rural areas of Northeast Brazil

O Sertão educa

Para la construcción de la tesis, reflexionamos sobre el sertão como un lugar que educa. Basado en la filosofía de la naturaleza, Merleau-Ponty, en alguna literatura del país, libros sobre la obra del poeta de la canción (João Batista de Siqueira), en las directrices, en conversaciones con los bosquimanos do Cariri paraibano y Pajeú Pernambuco, es posible pensar y entender la educación por la relación humana del interior con la naturaleza y la poesía. En el campo de la reflexión epistemológica, entendemos que la educación para la vida puede ser una ruta para entender el hombre de aprendizaje por medio de las relaciones y la construcción de un conocimiento compartido y experimentado a diario al nuevo aprendizaje. Involucrados con el fenómeno de Bush, la actitud fenomenológica era de fundamental importancia para poder caminar en los caminos de la investigación, siempre teniendo cuidado para no ser un alojamiento antes de que el fenómeno investigado, pero sí, un punto de partida para la construcción de horizontes de sentido, dando otros significados para una mejor aproximación. Es posible entender que la educación es la experiencia vivida de los sertanejos con la naturaleza y la poesía. Esta perspectiva fenomenológica revela que la educación de Bush emerge del mundo vivió de la montaña, a través del contacto sensible con otros animales, las plantas y la poesía. Es una educación corporal, como sucede en el universo de sensibilidad y abre el campo de la existencia humana, interconectado con los seres animados e inanimados do sertão

Efeito da luz da temperatura na germinação de sementes de aroeira (Myracrodruon urundeuva Allemão)

Estudou-se a germinação das sementes de aroeira (Myracrodruon urundeuvaAllemão) na presença e na ausência de luz, submetidas às temperaturas constantes de 10, 15, 20, 25, 30, 35 e 40 ºC e alternadas de 20-25, 20-30 e 20-35 ºC. Os parâmetros avaliados foram a porcentagem, a velocidade e a freqüência relativa de germinação. As sementes de aroeira germinaram na presença e na ausência de luz, mas se revelaram fotoblásticas negativas preferenciais. A germinação foi nula a 10 e a 40 ºC, e a faixa de germinabilidade foi de 15 a 35 ºC. No regime de temperatura constante, a faixa ótima para germinação das sementes foi de 20 a 30 ºC. No regime de temperatura alternada, a melhor flutuação térmica para germinação das sementes foi a de 20-30 ºC.

Propagação vegetativa de aroeira-pimenteira (Schinus terebinthifolius Raddi)

Este trabalho foi realizado com o objetivo de avaliar os melhores métodos e épocas de realização da enxertia, sobre o pegamento de enxertos de aroeira-pimenteira (Schinus terebinthifolius Raddi). Foram conduzidos dois experimentos, instalados na Faculdade de Ciências Agronômicas, da Universidade Estadual Paulista - Câmpus de Botucatu-SP, nos períodos de 16 de dezembro de 2005 a 30 de janeiro 2006 e de 03 de junho a 17 de julho de 2006. O delineamento utilizado foi de blocos casualizados, com cinco tratamentos, três repetições cada e dez plantas por parcela. Os tratamentos foram: garfagem no topo em fenda cheia, inglês simples, inglês complicado; borbulhia em T normal e T invertido. As diferentes épocas do ano alteraram o índice de pegamento, o que restringe o período de coleta de garfos. A maior porcentagem de pegamento ocorreu no mês de dezembro, com a enxertia em fenda cheia (40%). No mês de junho, não houve pegamento significativo, mostrando então que o mês de dezembro é o mês ideal, entre os estudados, para a realização da enxertia em aroeira. As garfagens em geral foram superiores à borbulhia. Houve baixo índice de pegamento, quando não foi nulo, e, além disso, suas borbulhas não apresentaram brotos dentro de 45 dias. A maior porcentagem de plantas com brotos, aos 45 dias, foi pelo método inglês simples, com 92%.

Abordando as relações ctsa no ensino da química a partir das crenças e atitudes de licenciandos: uma experiência ormativa no Sertão Nordestino

Este estudio investigó las actitudes y creencias de los estudiantes universitarios en Ciencias Químicas por la Universidad de Río Grande do Norte (UERN) como un paso hacia la elaboración de una propuesta de material didáctico para el estudio de las relaciones CTSA en la licenciatura en química. Para el conocimiento de las actitudes y creencias fue utilizado dos instrumentos (Escala Likert y un cuestionario), que proporcionó un análisis dentro del nuevo paradigma de investigación (cuantitativa y cualitativa) en la Enseñanza de la Ciencia y Matemáticas. Los resultados mostraron que los estudiantes de licenciatura en química tienen creencias positivistas y que suelen son considerados por algunos como ingenuas, como la creencia en el modelo de decisión tecnocrática, la linealidad del desarrollo científico y tecnológico que lleva a el bienestar social y neutralidad de los conocimientos científicotecnológico. Basándose en estos datos y la literatura sobre el tema se elaboraron tres módulos de aprendizaje: a) Cuestionando la hegemonía del conocimiento técnico y científico, b) Las relaciones entre ciencia, tecnología, sociedad y medio ambiente - las relaciones CTSA c) Propuesta de temática para la enseñanza de la química en una perspectiva de CTSA: El Río Mossoró. Por último, el material producido se utilizó en dos cursos ocurridos en el Campus Central en el Centro de Educación Superior en São Miguel, ambos de UERN. Los participantes expresaron su aprobación al enfoque trajo de el experimento propuesto y de el caso de simulación contenidos en la propuesta de

O significado dos símbolos e dos signos de noites do sertão

Les symboles et les signes de Noites do Sertão, de João Guimarães Rosa, contient l étude d une édition de cette ouvrage, publié par Livraria José Olímpio Editora, en 1975. Un éditeur arrêtée. Notre priorité c est montrer au lecteur les dégradations résultants d altérations ou d omissions qu on vérifie dans les publications postérieurs; d une manière spécifique, les éditions mais récents. Dans le primiere chapitre, notre recherche privilégie l analyse et l explication de las inscirtions qui sons enregistré dans la partie exterior, ou dans la couverture de ce livre. Le deuxième chapitre traite des réflexions sur ce qui se réfère au revers de la couverture, dans le côté gauche: les énigmes dont l auteur utilise avec l intention de bien idiquer au lecteur orientations pour envisager les aspects des narratives. Combien, nous méditerons ici sur la signification des épigraphes de cette ouvrage; sur les noms des personnages les plus importants et sur la connexion de ce livre avec autres textes littéraires. Dans le troisième chapitre nous explicitons la rélation de contiguïté avec cette ouvrage et le platonisme. O Chapitre quatre s occupe d étudier toutes cettes symboles vérifier ici sur la pérspective de la vision Junguienne (C.G.Jung); et dans le cinq, analysons les relations possibles de cette ouvrage avec autres livres du même écriteur. Finalement, nous présentons un vocabulaire qui est très importants pour la compréhention des mots mais compliqué dans ce texte écrit pour João Guimarães Rosa

Grande Sertão: Veredas e Don Quijote de la Mancha: melancolia em trânsito

Esta disertación aborda la lectura e interpretación de las obras Grande Sertão: Veredas, de João Guimarães Rosa y Del Ingenioso Hidalgo Don Quijote de la Mancha, de Miguel de Cervantes Y Saavedra, enfocando aspectos relacionados a las acciones de los caballeros andantes Riobaldo, el jagunço de Rosa y don Quijote, el Caballero de Cervantes, las cuales tuvieron decisiva influencia en su estado de espíritu melancólico. En la base teórica de este trabajo, investigamos la noción de melancolía, sistematizada por Kristeva y Freud, así como los estudios de la melancolía y de la alegoría a la luz del pensamiento de Walter Benjamin. Este estudio se concentra, sobre todo, en el aspecto que denominamos de alegoría de la circularidad, que permea las dos novelas, mientras expresión de cosas inacabadas que sugiere la idea de movimientos de espiralamento. Se percibió que lo retorno es consagrado en ambas las obras, es decir diferente el igual, dicho que consagra la diferencia y cierra las puertas para la repetición literal de sentido. En tal comprensión, ese movimiento no puede ser concebido como metáfora del mismo, pues sugiere la travesía que permanece inacabada y tortuosa, su estatuto de repetición no se honda en el encuentro de las dos puntas del ovillo, porque gira en el interior de aquello que repite como diferencia y liberación

Do teatro Elisabetano ao sertão do século XIX: a presença de Shakespeare em Inocência

The Shakespearean theater is a result of the genius of this playwright alongside the material provided by the period in which they came out the Elizabethan Age. Most of his works bring up themes and elements which keep them up-to-date, arousing an ongoing interest of readers and theatergoers, and also serving as inspiration for other writers to create their own works. Taking these ideas into account, this work aims to bring up questions concerning the presence of Shakespeare in a nineteenth-century Brazilian novel, Inocência, by Viscount of Taunay. In this novel, Taunay makes references to this playwright, using some epigraphs taken from Romeo and Juliet, from which we seek to understand how the novel dialogues with this Shakespearean drama. In order to develop such a study, we take into account some theoretical assumptions of hypertextuality, as proposed by the French scholar Gerard Genette, whose ideas about the dialogue between literary works support the analysis of the relationship between Taunay s novel and the above-referenced play of Shakespeare.

O padrão discursivo provérbio numa perspectiva cognitivo-experimental:expressões Idiomáticas em Grande Sertão: Veredas

Este estudio pretende dilucidar las motivaciones que llevan a los lectores del romance Grande Sertão: Veredas a la categorización de ciertas construcciones gramaticales que se encuentran en la obra como proverbiales. Para ello, recurren a la investigación de los procesos cognitivos implicados en la configuración del patrón discursivo proverbio, teniendo como soporte teórico a la Lingüística Cognitiva. En este empeño, nos ancoramos en las nociones de construcciones corporeizadas, simulación mental, frecuencia, patrón discursivo y en la expresión idiomática. Suponemos que los proverbios constituyen un patrón discursivo cristalizado a partir de la recurrencia de uso y, en virtud de eso, indagamos: ¿Qué mecanismos cognitivos son activados por los lectores en el proceso de categorización de las expresiones dichas proverbiales? Motivados por la situación problema presentada, formulamos algunos experimentos con la intención de aclarar las cuestiones investigadas y concluimos que los lectores recurren a constituciones construccionales subyacentes a los proverbios conocidos a través de las interacciones realizadas en su entorno sociocultural, para dar cuenta de la semántica de construcciones inéditas. En ese proceso, los esquemas y frames se activan a través de simulaciones mentales instanciadas por experiencias corporales y culturales, decisivas para la concretización de los procesos de las construcciones proverbiales