Anthranilic acid oxidase system of Tecomastans—III.: Studies on the conversion of o-aminophenol to catechol

The terminal step in the oxidation of anthranilic acid to catechol by anthranilic acid oxidase system from Tecoma stans, which converts o-aminophenol to catechol has been studied in detail. The reaction catalyses the conversion of one molecule of o-aminophenol to one molecule each of ammonia and catechol. The partially purified enzyme has a pH optimum of 6·2 in citrate-phosphate buffer and a temperature optimum of 45°. The metal ions, Mg2+, Co2+ and Fe3+ were inhibitory to the reaction. Metal chelating agents like 8-hydroxyquinoline, o-phenanthroline, and diethyldithiocarbamate, caused a high degree of inhibition. A sulfhydryl requirement for the reaction was inferred from the inhibition of the reaction by p-chloromercuribenzoate and its reversal with GSH. Atebrin inhibition was reversed by addition of FAD to the reaction mixture.

Electrochemical insertion of Sr2+ ions onto nano delta-MnO2 particles

Manganese dioxide is known to be an important electroactive material for supercapacitors. Generally, delta-MnO2 is subjected to electrochemical characterization studies in aqueous electrolytes of Na2SO4. It exhibits capacitance behaviour in the potential range between 0 and 1.0 V vs. SCE (saturated calomel electrode). In the present study, it is shown that delta-MnO2 exhibits capacitance behaviour in Sr(NO3)(2) electrolytes also. The suitable potential range in this electrolyte is also found to be 0-1.0 V. Specific capacitancemeasured in Sr(NO3)(2) electrolyte is 192 F g(-1). X-ray photoelectron spectroscopy data confirm that Sr2+ ions get inserted onto delta-MnO2 anoparticles. (C) 2010 Elsevier B.V. All rights reserved.

Amorphous Mesoporous Iron Aluminophosphate Catalyst for the Synthesis of 1,5-Benzodiazepines

A simple and versatile method for the synthesis of 1,5-benzodiazepines from o-phenylenediamine and ketones in the presence of solvents and under solvent-free conditions that used an amorphous mesoporous iron aluminophosphate as catalyst was developed. High yields with excellent selectivity were obtained with a wide variety of ketones under mild reaction conditions. The catalyst had the advantages of ease of preparation, ease of handling, simple recovery, reusability, non toxicity, and being inexpensive.

Carbohydrate-appended photocytotoxic (imidazophenanthroline)-oxovanadium(IV) complexes for cellular targeting and imaging

Oxovanadium(IV) complexes VO(aip)(L)](ClO4)(2) (L = phtpy, 1; stpy, 2) and VO(pyip)(L)](ClO4)(2) (L = phtpy, 3; stpy, 4), where aip is 2-(9-anthryl)-1H-imidazo4,5-f]1,10] phenanthroline, pyip is 2-(1-pyrenyl)-1Himidazo4,5-f]1,10] phenanthroline, phtpy is (4'-phenyl)-2,2': 6',2 `'-terpyridine and stpy is (2,2': 6', 2 `'-terpyridin-4'-oxy) ethyl-beta-D-glucopyranoside, were prepared, characterized and their DNA binding and photocleavage activity, cellular uptake and photocytotoxicity in visible light were studied. The complexes are avid binders to calf thymus DNA (K-b similar to 10(5) mol(-1)). They efficiently cleave pUC19 DNA in red light of 705 nm via the formation of HO center dot species. The glucose appended complexes 2 and 4 showed higher photocytotoxicity in HeLa and Hep G2 cells over the normal HEK 293T cells. No such preference was observed for the phtpy complexes 1 and 3. No significant difference in IC50 values was observed for the HEK 293T cells. Cell cycle analysis showed that the glucose appended complexes 2 and 4 are more photocytotoxic in cancer cells than in normal cells. Fluorescence microscopy was done to study the cellular localization of complex 4 having a pendant pyrenyl group.

A Closer Look at Multiple Forking: Leveraging (In)Dependence for a Tighter Bound

Boldyreva, Palacio and Warinschi introduced a multiple forking game as an extension of general forking. The notion of (multiple) forking is a useful abstraction from the actual simulation of cryptographic scheme to the adversary in a security reduction, and is achieved through the intermediary of a so-called wrapper algorithm. Multiple forking has turned out to be a useful tool in the security argument of several cryptographic protocols. However, a reduction employing multiple forking incurs a significant degradation of , where denotes the upper bound on the underlying random oracle calls and , the number of forkings. In this work we take a closer look at the reasons for the degradation with a tighter security bound in mind. We nail down the exact set of conditions for success in the multiple forking game. A careful analysis of the cryptographic schemes and corresponding security reduction employing multiple forking leads to the formulation of `dependence' and `independence' conditions pertaining to the output of the wrapper in different rounds. Based on the (in)dependence conditions we propose a general framework of multiple forking and a General Multiple Forking Lemma. Leveraging (in)dependence to the full allows us to improve the degradation factor in the multiple forking game by a factor of . By implication, the cost of a single forking involving two random oracles (augmented forking) matches that involving a single random oracle (elementary forking). Finally, we study the effect of these observations on the concrete security of existing schemes employing multiple forking. We conclude that by careful design of the protocol (and the wrapper in the security reduction) it is possible to harness our observations to the full extent.

Serrated Plastic Flow in a Zr-Based Bulk Metallic Glass During Nanoindentation

We investigate plastic deformation of Zr41.2Ti13.8Cu12.5Ni10Be22.5 bulk metallic glass using depth sensing nanoindentation. Numerous serrations in the load-displacement curves during indentation, shear bands and pile-ups around the indent were observed. The results revealed that the serrated plastic flow behaviour in this alloy depends strongly on the indentation strain rate.

Efecto de tres dietas suplementarias sobre parámetros productivos de cabras en crecimiento

Con el propósito de determinar el efecto de tres dietas suplementarias sobre el crecimiento post destete en cabras, bajo condiciones de pastoreo controlado, se estableció un ensayo, en un área de la Facultad de Ciencia Animal de la Universidad Nacional Agraria. Los tratamientos consistieron en una mezcla de Semolina, Melaza y Pecutrin en distintos niveles, de acuerdo a los requerimientos de los animales. El tratamiento T1, consistió en un 25 % menos del requerimiento óptimo, T2 consistió en los requerimientos óptimos y T3 en base al 25 % por encima del requerimiento óptimo. Las unidades experimentales fueron tres cabras con características de la raza criolla. Se utilizó un Diseño Cuadrado Latino (DCL) de 3 filas x 3 columnas (Períodos x Cabras). Las variables estudiadas fueron, ganancia media diaria (GMD), consumo de suplemento (CD) y la covariable peso inicial (PI). Posterior al ANDEVA, se realizaron separaciones de medias a través de la prueba de Duncan, para la variable consumo de suplemento. El período experimental fue de 63 días, divididos en 3 sub-períodos, teniendo cada uno una duración de 21 días, de los cuales, siete días correspondieron al periodo de adaptación y catorce días al período de evaluación. De los resultados, se encontró diferencias significativas (P<0.05) entre tratamientos, para la variable consumo alimenticio, no así para la variable ganancia media diaria. La covariable PI no ejerció efecto significativo sobre GMD. Los promedios obtenidos para CD y GMD fueron de 214, 310, 364, g/día y 59.52, 59.52, 17.86 g/día para TI,T2 y T3, respectivamente. El mayor valor para CD lo mostró T3, no así para GMD, donde TI y T2, presentaron los mayores promedios. Las tendencias de peso vivo y la influencia de los tratamientos sobre el crecimiento durante el periodo experimental, resultaron positivas.

Os fundos setoriais de ciência e tecnologia e seu impacto sobre o setor

Consultora Legislativa - Área XIV - Comunicação Social, Informática, Telecomunicações, Sistema Postal, Ciência e Tecnologia.

PICES-GLOBEC International Program on Climate Change and Carrying Capacity: Summary of the 1998 MODEL, MONITOR and REX Workshops, and Task Team Reports.

This volume summarizes the results of three workshops organized by the PICES-GLOBEC Climate Change and Carrying Capacity Program that were held just prior to the PICES Seventh Annual Meeting in Fairbanks, Alaska, in October 1998. These workshops represent the efforts of the REX, MODEL, and MONITOR Task Teams to integrate the results of national GLOBEC and GLOBEC-like programs to arrive at a better understanding of the ways in which climate change affects North Pacific ecosystems. (PDF contains 91 pages)

TWO-DIMENSIONAL ARRAY OF ION TRAPS ON A PLANAR CHIP

We investigate a planar ion chip design with a two-dimensional array of linear ion traps for the scalable quantum information processor. The segmented electrodes reside in a single plane on a substrate and a grounded metal plate, a combination of appropriate rf and DC potentials are applied to them for stable ion confinement, and the trap axes are located above the surface at a distance controlled by the electrodes' lateral extent and the substrate's height as discussed. The potential distributions are calculated using static electric field qualitatively. This architecture is conceptually simple and many current microfabrication techniques are feasible for the basic structure. It may provide a promising route for scalable quantum computers.

MODIFIED DIRECT TWOS-COMPLEMENT PARALLEL ARRAY MULTIPLICATION ALGORITHM FOR COMPLEX MATRIX OPERATION

A direct twos-complement parallel array multiplication algorithm is introduced and modified for digital optical numerical computation. The modified version overcomes the problems encountered in the conventional optical twos-complement algorithm. In the array, all the summands are generated in parallel, and the relevant summands having the same weights are added simultaneously without carries, resulting in the product expressed in a mixed twos-complement system. In a two-stage array, complex multiplication is possible with using four real subarrays. Furthermore, with a three-stage array architecture, complex matrix operation is straightforwardly accomplished. In the experiment, parallel two-stage array complex multiplication with liquid-crystal panels is demonstrated.

五自由度纳米级定位工作台的设计研究

提出一种新型的五自由度精密定位平台的工作原理及其设计方法。工作台采用柔性导向机构实现平移及转动功能，采用压电陶瓷作为驱动元件，外置纳米级电容传感器作为位移量测量反馈元件，采用数字PID控制方法，可以实现纳米级精度的定位。给出了多种形式柔性导向机构刚度计算公式及设计实例。

Estrelas dantescas nas galáxias de Haroldo de Campos

Uma constante no trabalho plural de Haroldo de Campos é o apreço pela intertextualidade. Em um percurso que toca as mais distantes e distintas galáxias, destacamos uma constelação: quatro textos literários (Finismundo: a última viagem, Signância quase céu, Galáxias e A máquina do mundo repensada) que entram em diálogo com a Commedia, de Dante Alighieri, e uma tradução de Haroldo de seis cantos do paraíso. Desdobrando o interesse de Haroldo de Campos pelo concreto da obra, partimos de algumas soluções formais dos textos em questão para pensar a experiência estética e a diferença entre as visões de mundo das obras comparadas.O estudo da Commedia permite perceber configurações formais que aproximam o leitor da experiência mística da personagem ao apresentá-la como experiência estética. O interesse de Haroldo de Campos está no potencial estético da obra de Dante. Confrontando o texto do poeta italiano com a tradução e com os quatro poemas citados acima, mostramos as semelhanças e diferenças dos processos de tradução e mimesis nas diversas metamorfoses pelas quais a Commedia passa nas criações de Haroldo de Campos