Development of a process for the production and purification of alpha- and beta-galactooligosaccharides from Bifobacterium bifidum NCIMB 41171

Synthesis of prebiotic alpha- and beta-galactooligosaccharides (GOS) using the whole cells of Bifidobacterium bifidum NCIMB 41171 was investigated. Determination of alpha- and beta-galactosidase activities showed them to be at 3 and 205 g(-1) of freeze dried biomass, respectively, and they increased to 5 and 344 U g(-1), respectively, when cells were treated with toluene. Starting with 450-500 mg mL(-1) lactose, maximum GOS concentrations were observed at 80-85% lactose conversions and the mixtures contained oligosaccharides (with a degree of polymerisation >= 3) at 77-109 mg mL(-1) and trans-galactosylated disaccharides between 85-115 mg mL(-1). The GOS yield values varied between 36% and 43%. An alpha-linked disaccharide was detected and its presence was confirmed by gas chromatography mass spectroscopy. Cells were re-used up to 8 times without changes in reaction times or the substrate conversions to GOS. Oligosaccharide synthesis was not inhibited by the presence of glucose or galactose. The mixtures were successfully purified from glucose (92% of glucose removed) by fermentation with Saccharomyces cerevisiae with no losses in the oligosaccharide content and only a small decrease on the galactose. (c) 2006 Elsevier Ltd. All rights reserved.

Os robots e a geometria dinâmica no ensino das funções no 7º Ano de Escolaridade: um estudo de caso

Nesta dissertação começo por fazer uma pequena reflexão sobre a minha experiência profissional. Seguindo-se a abordagem teórica que esteve como suporte para o desenvolvimento do meu estudo de caso, implementado nas minhas quatro turmas de 7º ano, num total de 109 alunos. Este estudo, baseou-se na aplicação de duas tarefas na unidade temática das Funções utilizando as tecnologias, nomeadamente, do robot MINDSTORMS® NXT da LEGO® e do software de geometria dinâmica GeoGebra como ferramentas de introdução e consolidação de conteúdos. O propósito desta tese de mestrado centra-se na importância e na relevância da utilização destas tecnologias no ensino da Matemática, no caso específico das Funções no 7º ano. A recolha e análise dos dados para a elaboração desta tese foram baseadas exclusivamente na vivência dos alunos aquando da realização das tarefas propostas, através da grelha de observação diária, da gravação em áudio e vídeo, de fotografias, das fichas de trabalho e dos questionários, visto ter optado por uma investigação qualitativa em que a participação dos intervenientes é fundamental. Foi evidente uma aceitação positiva por parte dos alunos a estas tecnologias. Assim sendo, permitiu-me concluir que realmente a aprendizagem dos mesmos foi significativa visto que a utilização destas ferramentas facilitou a aprendizagem de alguns conceitos abordados na unidade temática das Funções.

Resolución 648(XXXIII): Programa de trabajo de la Comisión Económica para América Latina y el Caribe para el bienio 2012-2013.

Incluye Bibliografía

Resolution 648(XXXIII) Programme of work of the Economic Commission for the 2012-2013 biennium

Includes bibliography

Simbióticos e ionóforo em dietas para bovinos mestiços Angus: desempenho, características de carcaça e qualidade de carne

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Selective uptake, distribution, and redistribution of Cd-109, Co-57, Zn-65, Ni-63, and Cs-134 via xylem and phloem in the heavy metal hyperaccumulator Solanum nigrum L

The focus of this article was to explore the translocation of Cd-109, Co-57, Zn-65, Ni-63, and Cs-134 via xylem and phloem in the newly found hyperaccumulator Solanum nigrum L. Two experiments with the uptake via the roots and transport of Cd-109, Co-57, and Zn-65 labeled by roots, and the redistribution of Cd-109, Zn-65, Co-57, Ni-63, and Cs-134 using flap label in S. nigrum in a hydroponic culture with a standard nutrient solution were conducted. The results showed that Cd-109 added for 24 h to the nutrient medium of young plants was rapidly taken up, transferred to the shoot, and accumulated in the cotyledons and the oldest leaves but was not efficiently redistributed within the shoot afterward leading to a rather low content in the fruits. In contrast, Co-57 was more slowly taken up and released to the shoot, but afterward, this element was redistributed from older leaves to younger leaves and maturing fruits. Zn-65 was rapidly taken up and transferred to the shoot (mainly to the youngest leaves and not to the cotyledons). Afterward, this radionuclide was redistributed within the shoot to the youngest organs and finally accumulated in the maturing fruits. After flap labeling, all five heavy metals tested (Cd-109, Co-57, Zn-65, Ni-63, Cs-134) were exported from the labeled leaf and redistributed within the plant. The accumulation in the fruits was most pronounced for Ni-63 and Zn-65, while a relatively high percentage of Co-57 was finally found in the roots. Cs-134 was roughly in the middle of them. The transport of Cd-109 differed from that previously reported for wheat or lupin and might be important for the potential of S. nigrum to hyperaccumulate cadmium.