Determination by MAD-DM of the structure of the DNA duplex d[ACGTACG(5-BrU)]2 at 1.46 Å and 100 K

A four-wavelength MAD experiment on a new brominated octanucleotide is reported here. d[ACGTACG(5-BrU)], C77H81BrN30O32P7, (DNA) = 2235, tetragonal, P43212 (No. 96), a = 43.597, c = 26.268 Å, V = 49927.5 Å3, Z = 8, T = 100 K, R = 10.91% for 4312 reflections between 15.0 and 1.46 Å resolution. The self-complementary brominated octanucleotide d[ACGTACG(5-BrU)]2 has been crystallized and data measured to 1.45 Å at both 293 K and a second crystal flash frozen at 100 K. The latter data collection was carried out to the same resolution at the four wavelengths 0.9344, 0.9216, 0.9208 and 0.9003 Å, around the Br K edge at 0.92 Å and the structure determined from a map derived from a MAD data analysis using pseudo-MIR methodology, as implemented in the program MLPHARE. This is one of the first successful MAD phasing experiments carried out at Sincrotrone Elettra in Trieste, Italy. The structure was refined using the data measured at 0.9003 Å, anisotropic temperature factors and the restrained least-squares refinement implemented in the program SHELX96, and the helical parameters are compared with those previously determined for the isomorphous d(ACGTACGT)2 analogue. The asymmetric unit consists of a single strand of octamer with 96 water molecules. No countercations were located. The A-DNA helix geometry obtained has been analysed using the CURVES program.

Population parameters in Brazilian Thoroughbred

The information in this study has been provided by the Brazilian Association of Racehorse Breeders [Associação do Brasileira dos Criadores do Cavalo de Corrida (ABCCC)]. It can be found in the files on the CD-ROM developed by the ABCCC in 1999. A total of 5008 finishing time records related to 2545 winning horses that ran in the classical calendar on Brazilian hippodromes during 25 years (197498) were analysed. There were a total of 9949 horses on the relationship matrix. The variance components were estimated using the multiple-trait derivate-free restricted maximum likelihood (MTDFREML) program, for an animal model. Generation intervals were higher in the maternal side (10.91 years) than in the paternal one (10.41 years). The estimates for genetic permanent environmental and phenotypic variances and heritability were 0.291, 0.161, 3.486 and 0.08, respectively. The phenotypic standard deviation for time in races was 1.86729 s. Genetic time trend on Thoroughbred races in Brazil was small and could be accelerated if selection considered the trait time effectively. With respect to the animal's country of birth, the results show that there has been an intense participation of foreign animals in breeding Brazilian Thoroughbreds.

A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phenolic Composition of the Edible Parts (Flesh and Skin) of Bordo Grape (Vitis labrusca) Using HPLC-DAD-ESI-MS/MS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biologia do ácaro Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) em condições de campo

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Efeito da aspiração folicular sobre a concentração de progesterona plasmática em éguas cíclicas

Pós-graduação em Medicina Veterinária - FMVZ

Medical adverse events in elderly hospitalized patients: A prospective study

OBJECTIVES: To determine the frequency of medical adverse events in elderly patients admitted to an acute care geriatric unit, the predictive factors of occurrence, and the correlation between adverse events and hospital mortality rates. METHODS: This prospective study included 171 admissions of patients aged 60 years and older in the acute care geriatric unit in a teaching hospital in Brazil between 2007 and 2008. The following variables were assessed at admission: the patient age, gender, number of prescription drugs, geriatric syndromes (e. g., immobility, postural instability, dementia, depression, delirium, and incontinence), comorbidities, functional status (evaluated with the Katz Index of Independence in Activities of Daily Living), and severity of illness (evaluated with the Simplified Acute Physiology Score II). The incidence of delirium, infection, mortality, and the prescription of potentially inappropriate medications (based on the Beers criteria) were assessed during hospitalization. An observer who was uninvolved in patient care reported the adverse events. RESULTS: The mean age of the sample was 78.12 years. A total of 187 medical adverse events occurred in 94 admissions (55%). The predictors of medical adverse events were undetermined. Compared with the patients with no adverse events, the patients with medical adverse events had a significantly longer hospital stay (21.41 +/- 15.08 days versus 10.91 +/- 7.21 days) and a higher mortality rate (39 deaths [41.5%] versus 17 deaths [22.1%]). Mortality was significantly predicted by the Simplified Acute Physiology Score II score (odds ratio [OR] = 1.13, confidence interval [CI] 95%, 1.07 to 1.20), the Katz score (OR = 1.47, CI 95%, 1.18 to 1.83), and medical adverse events (OR = 3.59, CI 95%, 1.55 to 8.30). CONCLUSION: Medical adverse events should be monitored in every elderly hospitalized patient because there is no risk profile for susceptible patients, and the consequences of adverse events are serious, sometimes leading to longer hospital stays or even death.

Hegemon y Strategos en Ayax de Sófocles

Es posible identificar en la tragedia Áyax de Sófocles la utilización de ciertos términos particulares empleados para referir al poder político y a las relaciones de poder entre los hombres que constituyen un medio por el cual el autor del drama realiza la historización y actualización del mito heredado del ciclo épico homérico. El mecanismo de historización del mito a través de la manipulación lingüística conduce a la "democratización" trágica de los homéricos y permite la puesta en escena de un debate político en términos contemporáneos al espectador de la tragedia. A partir del análisis de las apariciones del vocablo y otros términos relacionados, así como de las ocurrencias del término , el presente trabajo pretende acceder a una lectura que interprete la presencia de la hegemonía política como eje estructurador de la tragedia.

Hegemon y Strategos en Ayax de Sófocles

Es posible identificar en la tragedia Áyax de Sófocles la utilización de ciertos términos particulares empleados para referir al poder político y a las relaciones de poder entre los hombres que constituyen un medio por el cual el autor del drama realiza la historización y actualización del mito heredado del ciclo épico homérico. El mecanismo de historización del mito a través de la manipulación lingüística conduce a la "democratización" trágica de los homéricos y permite la puesta en escena de un debate político en términos contemporáneos al espectador de la tragedia. A partir del análisis de las apariciones del vocablo y otros términos relacionados, así como de las ocurrencias del término , el presente trabajo pretende acceder a una lectura que interprete la presencia de la hegemonía política como eje estructurador de la tragedia.

Hegemon y Strategos en Ayax de Sófocles

Es posible identificar en la tragedia Áyax de Sófocles la utilización de ciertos términos particulares empleados para referir al poder político y a las relaciones de poder entre los hombres que constituyen un medio por el cual el autor del drama realiza la historización y actualización del mito heredado del ciclo épico homérico. El mecanismo de historización del mito a través de la manipulación lingüística conduce a la "democratización" trágica de los homéricos y permite la puesta en escena de un debate político en términos contemporáneos al espectador de la tragedia. A partir del análisis de las apariciones del vocablo y otros términos relacionados, así como de las ocurrencias del término , el presente trabajo pretende acceder a una lectura que interprete la presencia de la hegemonía política como eje estructurador de la tragedia.

Social and immigration factors in intimate partner violence among Ecuadorians, Moroccans and Romanians living in Spain

Background: Intimate partner violence (IPV) against women occurs in all countries, all cultures and at every level of society; however, some populations may be at greater risk than others. The aim of this study was to explore IPV prevalence among Ecuadorian, Moroccan and Romanian immigrant women living in Spain and its possible association with their personal, family, social support and immigration status characteristics. Methods: Cross-sectional study of 1607 adult immigrant women residing in Barcelona, Madrid and Valencia (2011). Prevalence rates and adjusted odds ratios (AORs) were calculated, with current IPV being the outcome. Different women’s personal (demographic), family, social support and immigration status characteristics were considered as explicative and control variables. All analyses were separated by women’s country of origin. Results: Current IPV prevalence was 15.57% in Ecuadorians, 10.91% in Moroccans and 8.58% in Romanians. Some common IPV factors were found, such as being separated and/or divorced. In Romanians, IPV was also associated with lack of social support [AOR 5.96 (1.39–25.62)] and low religious involvement [AOR 2.17 (1.06–4.43)]. The likelihood of current IPV was lower among women without children or other dependants in this subgroup [AOR 0.29 (0.093–0.92)]. Conclusion: The IPV prevalence rates obtained for Moroccan, Romanian and Ecuadorian women residing in Spain were similar. Whereas the likelihood of IPV appeared to be relatively evenly distributed among Moroccan and Ecuadorian women, it was higher among Romanian women in socially vulnerable situations related to family responsibilities and the lack of support networks. The importance of intervention in the process of separation and divorce was common to all women.

Revista de educación

called <3.-5.> época; also called año <2-33>, época <6-97>; called no. <10-91>