Response of Iowa Pavements to a Tracked Agricultural Vehicle, HR-1075, Phase II, 2000

The overall objective of the work summarized in this report and in the interim report was to study the effects of targeted implement-of-husbandry loads. This report is to complement phase I of this work, which was summarized in the interim report, entitled Response of Iowa Pavements to Heavy Agricultural Loads (December 1999). The response of newly constructed Portland cement concrete (PCC) and asphalt cement concrete (ACC) pavements under semitruck, single-axle single-tire grain wagon, single-axle dual-tire grain wagon, tandem and tridem tank wagons were summarized in the interim report. Phase II of this project, presented herein, was to complete the study in terms of how tracked agricultural vehicles relate to the reference 20,000-pound single-axle semi-truck. In this report the response of these two pavements under a tracked grain wagon is documented.

Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth.

Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used atomic force microscopy (AFM) stiffness tomography to explore stiffness distribution in the cell wall of suspension-cultured Arabidopsis thaliana as a model of primary, growing cell wall. For the first time that we know of, this new imaging technique was performed on living single cells of a higher plant, permitting monitoring of the stiffness distribution in cell-wall layers as a function of the depth and its evolution during the different growth phases. The mechanical measurements were correlated with changes in the composition of the cell wall, which were revealed by Fourier-transform infrared (FTIR) spectroscopy. In the beginning and end of cell growth, the average stiffness of the cell wall was low and the wall was mechanically homogenous, whereas in the exponential growth phase, the average wall stiffness increased, with increasing heterogeneity. In this phase, the difference between the superficial and deep wall stiffness was highest. FTIR spectra revealed a relative increase in the polysaccharide/lignin content.

Deep learning via semi-supervised embedding

We show how nonlinear embedding algorithms popular for use with shallow semi-supervised learning techniques such as kernel methods can be applied to deep multilayer architectures, either as a regularizer at the output layer, or on each layer of the architecture. This provides a simple alternative to existing approaches to deep learning whilst yielding competitive error rates compared to those methods, and existing shallow semi-supervised techniques.

Avaliação de um modelo físico de produção de bovinos no semi-árido integrando caatinga, capim-buffel e leucena.: I. fase de cria

O estudo teve como objetivo avaliar um modelo físico do sistema de cria de bovinos para as áreas mais secas do Semi-Árido brasileiro, associando a pastagem natural com pastos cultivados tolerantes à seca. A caatinga foi pastejada no período verde, e o capim buffel no restante do ano. A suplementação com leucena ocorreu no período seco, sob pastejo direto e feno no cocho. Parâmetros de desempenho, monitorados de novembro de 1991 a outubro de 1995, mostraram uma taxa média de parição da ordem de 72,8% ao ano e taxas de mortalidade praticamente nulas. O peso vivo médio dos bezerros aos 205 dias de idade foi de 153,4 kg e a produ- ção de bezerros desmamados foi de 109,5 kg/matriz exposta/ano e de 25,5 kg/hectare/ano. Os resultados são considerados bastante expressivos, considerando-se que mais da metade da área era ocupada com pastagens nativas de caatinga e que a oferta de leucena foi limitada pela ocorrência de uma forte estiagem.

Uses of also in oral semi-informal German

In recent grammars and dictionaries also (`therefore, so, well¿) continues to be preferably presented as an adverb with a conclusive-consecutive connective function that essentially corresponds to its use in formal written German. Its function as a modal particle is documented, however, since the beginnings of what is known as Partikelforschung, though not all its uses have been systematically investigated contrasting oral and written German, either in mode or concept. In this article we analyse the uses of also in semi-informal oral interactions on the basis of empirical data (from a subsample of the VARCOM corpus). Specifically, we will analyse the presence and frequency of also at the beginning of a sentence or sequence, the functions it serves as a logical-semantic connector or discourse and interaction marker and the interrelations between these functions, in order to contrast these results with the description of also provided by current reference works.

SUPLEMENTAÇÃO ALIMENTAR DE CABRAS ANGLO-NUBIANAS NA ÉPOCA CHUVOSA, NA REGIÃO SEMI-ÁRIDA DO NORDESTE BRASILEIRO

Foi realizado um experimento para avaliar a influência da suplementação alimentar na produção de leite de cabras da raça Anglo-nubiana, durante a época chuvosa, na região semi-árida do Nordeste brasileiro. Vinte e quatro cabras foram mantidas em pastagem nativa rebaixada, distribuídas ao acaso, por tipo de parto e produção de leite, em três níveis de suplementação: N0 - sem suplementação, N1 -150 g de concentrado/animal/dia e N2 - 300 g de concentrado/animal/dia. Não houve diferença significativa (p>0,05) entre os níveis de suplementação. A produção de leite foi descrita por uma regressão múltipla em função do período de lactação em semana. A interação semana x níveis de suplementação foi significativa (p<0,01). Os resultados revelam que a suplementação com concentrado, na dose de 300 g/animal/dia, foi a melhor opção para se produzir leite com cabras Anglo-nubianas, durante a época chuvosa.

Cerebellar cortical layers: in vivo visualization with structural high-field-strength MR imaging.

Purpose: To perform in vivo imaging of the cerebellum with an in-plane resolution of 120 mm to observe its cortical granular and molecular layers by taking advantage of the high signal-to-noise ratio and the increased magnetic susceptibility-related contrast available at high magnetic field strength such as 7 T. Materials and Methods: The study was approved by the institutional review board, and all patients provided written consent. Three healthy persons (two men, one woman; mean age, 30 years; age range, 28-31 years) underwent MR imaging with a 7-T system. Gradient-echo images (repetition time msec/echo time msec, 1000/25) of the human cerebellum were acquired with a nominal in-plane resolution of approximately 120 mum and a section thickness of 1 mm. Results: Structures with dimensions as small as 240 mum, such as the granular and molecular layers in the cerebellar cortex, were detected in vivo. The detection of these structures was confirmed by comparing the contrast obtained on T2*-weighted and phase images with that obtained on images of rat cerebellum acquired at 14 T with 30 mum in-plane resolution. Conclusion: In vivo cerebellar imaging at near-microscopic resolution is feasible at 7 T. Such detailed observation of an anatomic area that can be affected by a number of neurologic and psychiatric diseases, such as stroke, tumors, autism, and schizophrenia, could potentially provide newer markers for diagnosis and follow-up in patients with such pathologic conditions. (c) RSNA, 2010.

Efeito da calagem e inoculação de sabiá em solo da mata úmida e do semi-árido de Pernambuco

Realizou-se um experimento, usando solo álico da Zona da Mata e do Semi-Árido do Estado de Pernambuco, com o objetivo de avaliar o efeito da calagem (0, 3 e 6 t ha-1) e da inoculação das estirpes de rizóbio: NFB 539, NFB 577 e NFB 578, previamente selecionadas para sabiá (Mimosa caesalpiniaefolia) em meio de cultura ácido. Adicionaram-se tratamentos sem inoculação, sem e com adição de N mineral (100 kg ha-1), para fins comparativos. O experimento seguiu o esquema fatorial 2x3x5, no delineamento em blocos ao acaso, com quatro repetições. As plantas foram colhidas 110 dias após a emergência (DAE). A inoculação de rizóbio, em todos os níveis de calagem, mostrou efeito significativo nos parâmetros avaliados. No nível de 3 t ha-1 não houve efeito da calagem, porém a adição de 6 t ha-1 de calcário reduziu o peso de matéria seca, o N total na parte aérea, a nodulação e a atividade da nitrogenase. O baixo pH e Al3+ do solo não prejudicou a fixação do N2, e o crescimento das plantas que receberam o inóculo. Portanto, torna-se desnecessária a calagem no cultivo de sabiá em solos ácidos, quando usadas estirpes selecionadas visando à resistência a acidez.

Effects of prior repeated-sprints with different recovery duration on oxygen uptake kinetics during subsequent heavy-exercise

Introduction: Prior repeated-sprints (6) has become an interesting method to resolve the debate surrounding the principal factors that limits the oxygen uptake (V'O2) kinetics at the onset of exercise [i.e., muscle O2 delivery (5) or metabolic inertia (3)]. The aim of this study was to compare the effects of two repeated-sprints sets of 6x6s separated by different recovery duration between the sprints on V'O2 and muscular de-oxygenation [HHb] kinetics during a subsequent heavy-intensity exercise. Methods: 10 male subjects performed a 6-min constant-load cycling test (T50) at intensity corresponding to half of the difference between V'O2max and the ventilatory threshold. Then, they performed two repeated-sprints sets of 6x6s all-out separated by different recovery duration between the sprints (S1:30s and S2:3min) followed, after 7-min-recovery, by the T50 (S1T50 and S2T50, respectively). V'O2, [HHb] of the vastus lateralis (VL) and surface electromyography activity [i.e., root-mean-square (RMS) and the median frequency of the power density spectrum (MDF)] from VL and vastus medialis (VM) were recorded throughout T50. Models using a bi-exponential function for the overall T50 and a mono-exponential for the first 90s of T50 were used to define V'O2 and [HHb] kinetics respectively. Results: V'O2 mean value was higher in S1 (2.9±0.3l.min-1) than in S2 (1.2±0.3l.min-1); (p<0.001). The peripheral blood flow was increased after sprints as attested by a higher basal heart rate (HRbaseline) (S1T50: +22%; S2T50: +17%; p≤0.008). Time delay [HHb] was shorter for S1T50 and S2T50 than for T50 (-22% for both; p≤0.007) whereas the mean response time of V'O2 was accelerated only after S1 (S1T50: 32.3±2.5s; S2T50: 34.4±2.6s; T50: 35.7±5.4s; p=0.031). There were no significant differences in RMS between the three conditions (p>0.05). MDF of VM was higher during the first 3-min in S1T50 than in T50 (+6%; p≤0.05). Conclusion: The study show that V'O2 kinetics was speeded by prior repeated-sprints with a short (30s) but not a long (3min) inter-sprints-recovery even though the [HHb] kinetics was accelerated and the peripheral blood flow was enhanced after both sprints. S1, inducing a greater PCr depletion (1) and change in the pattern of the fibres recruitment (increase in MDF) compared with S2, may decrease metabolic inertia (2), stimulate the oxidative phosphorylation activation (4) and accelerate V'O2 kinetics at the beginning of the subsequent high-intensity exercise.

Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida sobre o crescimento de Eucalyptus dunnii Maiden

O crescimento de Eucalyptus é favorecido por fungos ectomicorrízicos (FECM). De ocorrência comum nas plantações florestais, os FECM diferem, entretanto, em infectividade e eficiência. O objetivo deste estudo foi testar a compatibilidade e a eficiência do inoculante ectomicorrízico produzido por fermentação semi-sólida, em relação a Eucalyptus dunnii Maiden. Neste sentido, o inoculante de Pisolithus sp., produzido por fermentação semi-sólida em vermiculita-solução nutritiva, foi aplicado ao substrato turfa-vermiculita (30:70, v/v) nas concentrações de 0, 1, 3, 5 e 10% (v/v) que foi semeado com E. dunnii, e mantido em casa de vegetação. A inoculação proporcionou colonização radicular e aumento da matéria seca da parte aérea e do conteúdo de P em razão da concentração do inoculante. O peso da matéria seca das plantas com 1% de inoculante não diferiu do peso das testemunhas. A 3% e 5%, a matéria seca aumentou 62%. O maior valor foi obtido com 10% de inoculante, onde o peso das plantas foi 73% superior ao das testemunhas, e o teor de P, 130%. Resultados semelhantes foram obtidos em relação à raiz. Altura e diâmetro foram positivamente afetados pela inoculação, mas não a relação raiz/parte aérea. As doses mais eficientes de inoculante foram aquelas de 3% ou superiores. Este método de produção deverá ser testado com outros FECM, e sua eficiência, avaliada em viveiro e no campo.

Heavy metal accumulation by intestinal helminths of vertebrates

The relevancy of parasites as potential indicators of environmental quality has been increasing over the last years, mostly due to the variety of ways in which they respond to anthropogenic pollution. The use of fish parasites as bioindicators of heavy metal pollution in aquatic ecosystems has been widely studied. However, little information concerning terrestrial habitats is presently available. In fact, in the last two decades several studies have been performed worldwide in different habitats and/or conditions (theoretically both in polluted and unpolluted terrestrialecosystems, but mainly in aquatic ecosystems) in order to investigate heavy metal pollution using parasitological models. Different groups of vertebrates (mainly fish, mammals and birds) and several parasitological models have been tested involving acanthocephalans mostly, but also cestodes and nematodes. It is not the aim of this chapter to do a complete revision of the availabledata concerning this subject. Instead, we emphasize some general aspects and compile a mini-review of the work performed in this field by our research group. The results obtained until now allow confirming several parasitic models as promising bioindicator systems to evaluate environmental cadmium and mainly lead pollution in terrestrial non-urban habitats, as it was already demonstrated for aquatic ecosystems. The present knowledge also allows confirming that parasites can reveal environmental impact. Environmental parasitology is an interdisciplinary field, which needs simultaneous expertise from toxicology, environmental chemistry and parasitology. Furthermore, environmental parasitology should be taken into account in order to increase the efficiency of environmental monitoring programs.

Arsenic and heavy metal concentrations in agricultural soils in South Savo province

Selostus: Etelä-Savon viljelysmaan arseeni- ja raskasmetallipitoisuudet

Characterization of MAP1B heavy chain interaction with actin.

Microtubule-associated protein 1B is an essential protein during brain development and neurite outgrowth and was studied by several assays to further characterize actin as a major interacting partner. Tubulin and actin co-immunoprecipitated with MAP1B at similar ratios throughout development. Their identity was identified by mass spectrometry and was confirmed by Western blots. In contrast to previous reports, the MAP1B-actin interaction was not dependent on the MAP1B phosphorylation state, since actin was precipitated from brain tissue throughout development at similar ratios and equal amounts were precipitated before and after dephosphorylation with alkaline phosphatase. MAP1B heavy chain was able to bind actin directly and therefore the N-terminal part of MAP1B heavy chain must also contain an actin-binding site. The binding force of this interaction was measured by atomic force microscopy and values were in the same range as those of MAP1B binding to tubulin or that measured in MAP1B self-aggregation. Aggregation was confirmed by negative staining and electron microscopy. Experiments including COS-7 cells, PC12 cells, cytochalasin D and immunocytochemistry with subsequent confocal laser microscopy, suggested that MAP1B may bind to actin but has no obvious microfilament stabilizing effect. We conclude, that the MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation.

Heavy-metal pollution and remediation of forest soil around the Harjavalta Cu-Ni smelter, in SW Finland

Abstract