Estudo da expressão imuno-histoquímica das proteínas MMP-9, MMP-13 e TIMP-1 em ameloblastomas e tumores odontogênicos ceratocistos

Ameloblastomas and keratocystic odontogenic tumors (KOT) represent odontogenic lesions that, despite their benign nature, are distinguished by a distinct biological behavior, characterized by locally aggressive growth and recurrent episodes. The gnathic bone resorption caused by the growth of these lesions is a key to the expansion of the same, both being mediated by osteoclastic cells like enzymatic activity of various matrix metalloproteinases (MMPs) factor. The expression of stimulatory factors and inhibitors of bone resorption has been correlated with the development of these lesions, with emphasis to some MMPs such as collagenases and gelatinases and tissue inhibitors of metalloproteinases (TIMPs), among others. Based on the premise that stimulatory and inhibitory factors of osteolytic processes can be decisive for the growth rate of intraosseous odontogenic lesions, this experiment evaluated the immunoreactivity of MMP-9, -13 and TIMP-1 protein in the epithelium and mesenchyme of ameloblastoma and the KOT specimens, by a quantitative analysis of the immunoreactivity cells. Statistical analysis was performed using the Mann-Whitney and Wilcoxon tests with a significance level set at 5 %. Immunohistochemical expression of MMP-9, -13 and TIMP-1 was observed in 100% of cases both in the epithelium and in mesenchyme. The immunoreactivity in the epithelium of KOT and ameloblastomas revealed a predominance of score 3 for MMP-9 (p=0.382) and MMP-13 (p=0.069) and no statistically significance for TIMP-1, the latter being significantly higher immunoreactivity in ameloblastomas. In the mesenchyme, there was a higher score immunoreactivity of MMP-13 (p=0.031) in ameloblastomas in relation to KOT, whereas for MMP-9 and TIMP-1 no statistically significant difference (p=0.403 was observed, p=1.000). The calculation of the ratio of scores revealed expression of proteins in general, similarity of the lesions, a significant predominance of equal expression of TIMP-1 and MMP-9 was observed only in the epithelium of ameloblastoma. The marked immunostaining of MMP-9 , MMP-13 and TIMP-1 in epithelium and mesenchyme of the lesion indicate that these proteins involved in ECM remodeling required for tumor progression, however, specific differences in the expression of some of these proteins, are not sufficient to suggest differences in the biological behavior of ameloblastomas and KOTs

Comparação dos valores de desgaste abrasivo e de microdureza de 13 resinas compostas usadas em odontologia através do método do disco retificado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos didáticos, laicos e científicos de artigos de divulgação científica de duas revistas de conteúdo geral

Muitos professores buscam, em textos jornalísticos, revistas ou jornais, matérias com conteúdo científico para utilizarem como material de apoio em classe. Será que o conteúdo científico contido nesse material é, realmente, aproveitável? O discurso utilizado pelos jornalistas promove o conhecimento científico sobre os assuntos que discutem? Assim, este trabalho teve por objetivo analisar, quanto ao conteúdo científico, didático e acessível, matérias das revistas semanais de conteúdo geral mais lidas do país, Veja e Época, publicadas em 2008, com o tema Meio Ambiente. Os resultados permitiram concluir que os textos apresentados pela revista Veja promovem um discurso científico aproveitável do ponto de vista didático, podendo ser utilizados em sala de aula como material de apoio e complementar pelos professores.

Condição peri-implantar e fatores associados às doenças peri-implantares em pacientes reabilitados no serviço odontológico da faculdade de odontologia da UFRN

A introdução dos implantes dentários osseointegrados como uma ferramenta na reabilitação oral de pacientes edêntulos e parcialmente edêntulos é uma realidade no cotidiano do cirurgião-dentista. Estudos reportam uma alta taxa de sucesso da utilização de implantes no tratamento reabilitador. Entretanto, outras investigações têm mostrado a perda desses implantes devido a infecções peri-implantares, como a mucosite e a peri-implantite. O objetivo deste trabalho foi avaliar a frequência das doenças peri-implantares e seus fatores associados em pacientes com implantes dentais em função reabilitados no serviço odontológico da Faculdade de Odontologia da UFRN. Foram examinados 155 indivíduos portadores de 523 implantes e 2718 dentes. Dentes e implantes foram avaliados por meio de sondagem periodontal, observando-se a profundidade de sondagem, retração gengival, bem como foram avaliados índices de placa visível (IPV) e sangramento gengival (ISG) e presença de supuração. Os dados foram armazenados em fichas clínicas e avaliados estatisticamente por meio da estatística descritiva e inferencial. A idade média dos pacientes foi de 54,05 (± 12,61) anos, sendo 79,4% do sexo feminino. As frequências da mucosite, peri-implantite e periodontite em indivíduos foram 54%, 28% e 50%, respectivamente. Dos 523 implantes avaliados, 43% tinham mucosite, 14% peri-implantite e 43% saúde. Os testes Qui-quadrado de Pearson e Exato de Fisher mostraram que as doenças peri-implantares estão associadas as doenças periodontais, uso de medicação, alterações sistêmicas número de implantes, IPV, ISG, ao tempo de função das próteses, região do implante, número de roscas expostas e faixa de mucosa queratinizada (p<0,05). A análise de regressão múltipla, através da regressão binária logística, constatou que indivíduos que faziam uso de medicação (OR = 1,784), com um ISG > 10% (OR = 1,742), com implantes instalados na maxila (OR = 2,654), onde a prótese sobre o implante tinham mais de 2 anos em função (OR = 3,144) e que radiograficamente apresentavam uma perda óssea atingindo a terceira rosca do implante (OR = 4,701) mostram uma associação positiva com as doenças peri-implantares de maneira que esses indivíduos têm mais chances de ter essas doenças. Os resultados sugerem que a frequência das doenças peri-implantares na população em estudo foi de 82% dos pacientes e que estas doenças estão associadas a fatores relacionados aos indivíduos como: a presença da doença periodontal, piores IPV e IS, alterações sistêmicas, uso de medicação e maior número de implantes; e a fatores locais relacionados aos implantes como: ausência ou faixa de mucosa menor que 2mm, implantes na maxila e na região anterior, perda óssea atingindo a terceira rosca do implante e a um tempo de reabilitação prótetica maior que 2 anos

Projeto pedagógico: abordagens e implicações no âmbito da formação em odontologia

Proposals that aim a redirection of current health attention models in Brazil are indispensible due to the need of health issues and its challenges imposed by society. These issues come forth in Higher Education Institutions in an attempt to devise ways to face these demands. The research aims to analyze the Pedagogical Project (PP) present in the Dentistry Graduation Course in the Federal University of Rio Grande do Norte in Brazil-(UFRN). This is done in coherence the Brazilian National Curricular Guidelines considering main competences established in the PP. This research was approved by the Ethics Committee at UFRN under document number 285/201. The work is of descriptive nature and was realized with 30 students in the Dentistry Graduation Course. Interviews were realized with the use of problem situation approach. The research was also supported by documental studies that dealt with syllabus present in the disciplines taught at UFRN. Data were processed with the use of the ALCESTE 4.9 software. It is possible to acknowledge that some conservative conceptions arise, even though there is use of active methodologies and innovation that aim to promote reflection and articulation for general competence development such as proposed in the Pedagogical Project in Dentistry Graduation Course at UFRN. These conceptions are mainly present in the teaching-learning process where students do not have full participation. Thus it is possible to conclude that even though there are advances and breakthroughs. This is seen with that fact that there was inclusion of multidisciplinary clinical work as well optional courses in the curriculum. It was also seen that there was occasional use of active teaching methodologies in Dentistry at UFRN. But there is still a need for a didactical and methodological resizing. These actions require the need for progressive development of competences and abilities during the formative process according to what was established in the Brazilian National Curricular Guidelines

Fatores socioeconômicos contextuais associados à condição bucal de adolescentes no Brasil

Many surveys are conducted comparing oral health conditions with individual variables, such as socioeconomic and demographic factors. However, in the same way that individuals differ among themselves, the groups also have their own characteristics and the effects of this differentiation must be researched. Brazil, despite being one of the major economic powers of the world and shows an improvement in the average value of its health indicators, is also one of the most unequal and remains among the countries with the greatest health inequities. The purpose of this study was to investigate the importance of social determinants on the contextual level oral health among Brazilian adolescents, population not much researched by the literature. The research was made using an ecological approach in order to identify possible inequalities between cities and capitals. Using data from SBBrasil 2010 it was evaluated less common outcomes (loss of first molar, dental care index and T-Health) which provide information on the degree of morbidity of caries and health level of dental tissues, in addition to analyze the related services. The association of these oral health indicators with socioeconomic factors such as income, employment, education and inequality, collected from Census 2010, was analyzed by simple and multiple linear regressions. The study included the 27 state capitals and four clusters representing the municipalities of the country. It was possible to see better access to services in locations with better income distribution. However, the strong association of contextual factors related to poverty, low levels of education and poor housing and jobs with poorer levels of oral health in adolescents seems to overshadow the effects of income inequalities on dental caries in the country. In some locations, particularly within the North and Northeast, whichever one keeps dentistry mutilating, whose effects are already noticeable in its adolescent population. Access to restorative services in Brazil remains limited and unequal. The results of this study highlight the inequities in oral health in the country and show the need of the inclusion of new perspectives on the traditional approach of Preventive Dentistry and education models in Dentistry. Tackling health inequalities in oral health in the country requires the cooperation of various actors involved in the process and the inclusion of oral health in the context of overall health. The social determinants approach, as well as evaluating the distribution of oral diseases in the country and its inclusion in the context of overall health, should guide the implementation of programs and oral health practices in order to contribute to the reduction of inequalities

Bolton freeware: aplicativo de avaliação de discrepância de massa dentária desenvolvido pelo programa de pós-graduação em odontologia/UFRN

The calculation of tooth mass discrepancy, essential for good planning and a proper orthodontic finishing, when performed manually, besides being laborious, requires considerable time consumption. The aim of this study was to develop and test Bolton Freeware, a software for analysis of the tooth mass discrepancy of Bolton, aiming to minimize the consumption of time in a less onerous way. The digital analysis of the software was done by means of two-dimensional scanning of plaster study models and compared to manual evaluation (gold standard), using 75 pairs of stone plaster study models divided into two groups according to the magnitude of the Curve of Spee (group I from 0 to 2 mm, group II greater than 2 to 3mm). All the models had permanent dentition and were in perfect condition. The manual evaluation was performed with a digital caliper and a calculator, and the time required to perform the analysis for both methods was recorded and compared. In addition, the software was evaluated by orthodontists regarding its use, by means of questionnaires developed specifically for this purpose. Calibration was performed prior to manual analysis, and excellent levels of inter-rater agreement were achieved, with ICC > 0.75 and r > 0.9 for total and anterior proportion. It was observed in the evaluation of error of the digital method that some teeth showed a significant systematic error, being the highest measured at 0.08 mm. The analysis of total tooth mass discrepancy performed by Bolton Freeware, for those cases in which the curve of Spee is mild and moderate, differ from manual analysis, on average, 0.09 mm and 0.07 mm respectively, for each tooth evaluated, with r> 0, 8 for total and anterior proportion. According to the specificity and sensitivity test, Bolton Freeware has an improved ability to detect true negatives, i.e. the presence of discrepancy. The Bolton analysis digitally performed was faster, with an average difference of time consumed to perform the analysis of Bolton between the two methods of approximately 6 minutes. Most experts interviewed (93%) approved the usability of the software

Projeto de Sistemas Integrados de Propósito Geral Baseados em Redes em Chip Expandindo as Funcionalidades dos Roteadores para Execução de Operações: A plataforma IPNoSys

It bet on the next generation of computers as architecture with multiple processors and/or multicore processors. In this sense there are challenges related to features interconnection, operating frequency, the area on chip, power dissipation, performance and programmability. The mechanism of interconnection and communication it was considered ideal for this type of architecture are the networks-on-chip, due its scalability, reusability and intrinsic parallelism. The networks-on-chip communication is accomplished by transmitting packets that carry data and instructions that represent requests and responses between the processing elements interconnected by the network. The transmission of packets is accomplished as in a pipeline between the routers in the network, from source to destination of the communication, even allowing simultaneous communications between pairs of different sources and destinations. From this fact, it is proposed to transform the entire infrastructure communication of network-on-chip, using the routing mechanisms, arbitration and storage, in a parallel processing system for high performance. In this proposal, the packages are formed by instructions and data that represent the applications, which are executed on routers as well as they are transmitted, using the pipeline and parallel communication transmissions. In contrast, traditional processors are not used, but only single cores that control the access to memory. An implementation of this idea is called IPNoSys (Integrated Processing NoC System), which has an own programming model and a routing algorithm that guarantees the execution of all instructions in the packets, preventing situations of deadlock, livelock and starvation. This architecture provides mechanisms for input and output, interruption and operating system support. As proof of concept was developed a programming environment and a simulator for this architecture in SystemC, which allows configuration of various parameters and to obtain several results to evaluate it

Indexação automatizada de artigos de periódicos científicos: análise da aplicação do software SISA com uso da terminologia DeCS na área de Odontologia

The indexing automation has been discussed by researches in the area of Information Science however the discussions have not been so clear on the use of indexing software. Thus, it is necessary to know the indexing software, as well as its application in the analysis of documentary contents. To do so, it is proposed, here, to investigate both the consistency of indexing and the exhaustiveness and precision of the information retrieval, by means of comparative analysis between SISA (Sistema de Indizacion Semi-Automatico) automatic index and BIREME ( Centro Latino-Americano e do Caribe de Informação em Ciencias da Saude) manual indexing. The aim of this paper is to contribute to the theoretical development of the indexing automation and the improvement of SISA. Thus, SISA application and evaluation was used based on the calculation of the consistency indexes between the two types of indexing, and the calculation of the exhaustiveness and precision indexes in information retrieval, by means of searching into BDSISA and BIREME databases, composed by descriptors taken from SISA and manual indexing respectively. The differences among the terms used in scientific papers comparing to the DeCS ones were the main difficult factor to achieve higher consistency indexes in the indexing. These differences influenced the exhaustiveness and precision indexes in the information retrieval, showing that it is necessary to improve the documentary language used by SISA software and to incorporate linguistic methods.

Conhecimento sobre ergonomia no âmbito acadêmico: um estudo com alunos e professores de odontologia

OBJETIVO: As doenças osteomusculares são as afecções ocupacionais mais prevalentes em cirurgiões-dentistas. Nosso propósito: 1) investigar os conhecimentos, aplicabilidades clínicas dos princípios ergonômicos em discentes e docentes em atividades clínicas de uma universidade pública 2) pesquisar a incidência de sintomatologias dolorosas no pescoço, ombros, parte superior e inferior das costas, cotovelos, quadris, coxas, joelhos, tornozelos e pés no universo de alunos em estágios clínicos. 3) incitar discussões de normas e diretrizes ergonômicas na universidade. MÉTODOS: Esse estudo investigou o universo de alunos matriculados em disciplinas clínicas (148) e respectivos professores (30) do curso de odontologia da Universidade Federal do Rio Grande do Norte, Natal-RN a respeito dos princípios ergonômicos utilizados na rotina clínica. Paralelamente foi pesquisada a incidência de sintomatologia dolorosa nos alunos por intermédio do questionário nórdico e a partir dos resultados foi mensurado o índice de severidade dos sintomas em alunos. The Nordic Musculoskeletal Questionnaire (NMQ) é um instrumento de diagnóstico, proposto para padronizar a mensuração de relatos de sintomas osteomusculares. A análise dos dados foi através do programa SPSS-Statistical Package for the Social Sciences, versão 17.0 realizada analítica e descritivamente, com determinação das médias (x), desvio-padrão para variáveis quantitativas, freqüências simples e relativas para as variáveis categóricas, além da estatística de associação entre grupos (teste t) e a análise de associação do quiquadrado com nível de significância 5% entre as variáveis (Person). As respostas das questões abertas foram codificadas e transformadas em freqüências, descritas posteriormente. RESULTADOS: A aplicabilidade de medidas ergonômicas nas clínicas universitárias não foi evidenciada pelo universo de discentes e docentes. Quanto ao relato de sintomas osteomusculares o sexo feminino foi o mais acometido qualquer que seja o nível acadêmico cursado. As regiões anatômicas de maior grau de severidade de relatos dos sintomas foram: pescoço, parte inferior das costas, punhos, mãos e ombros, com significância etatística p<0,001. CONCLUSÃO: Em função dos achados os autores apresentam um protocolo de intervenção clínica baseado nos determinantes ergonômicos da Associação internacional de ergonomia (EAI) como medida de prevenção da saúde ocupacional dos futuros cirurgiões-dentistas ainda em processo de formação nas clínicas odontológicas das universidades.

Estimador do Tipo Núcleo para Densidades Limites de Cadeias de Markov com Espaço de Estados Geral

In this work we studied the consistency for a class of kernel estimates of f f (.) in the Markov chains with general state space E C Rd case. This study is divided into two parts: In the first one f (.) is a stationary density of the chain, and in the second one f (x) v (dx) is the limit distribution of a geometrically ergodic chain

Estimadores do tipo núcleo para a densidade de transição de uma cadeia de markov com espaço de estados geral

In this work, we studied the strong consistency for a class of estimates for a transition density of a Markov chain with general state space E ⊂ Rd. The strong ergodicity of the estimates for the density transition is obtained from the strong consistency of the kernel estimates for both the marginal density p(:) of the chain and the joint density q(., .). In this work the Markov chain is supposed to be homogeneous, uniformly ergodic and possessing a stationary density p(.,.)

A Grounded Theory como abordagem metodológica para pesquisas qualitativas em odontologia

Este trabalho consiste na proposta de utilização, em Odontologia, de uma abordagem metodológica usada em pesquisas qualitativas, denominada Grounded Theory, quando se objetiva compreender fenômenos ou se pretende descrevê-los de acordo com o ponto de vista do sujeito. Pesquisas em saúde, utilizando abordagens qualitativas, estão sendo, cada vez mais, empregadas entre pesquisadores e, na Odontologia, esta abordagem metodológica proposta torna-se fundamental, no sentido de propiciar a exploração de assuntos ainda pouco trabalhados nesta perspectiva metodológica, possibilitando que as pesquisas adquiriram contribuições originais e, assim, ampliando nosso conhecimento. São apresentadas as etapas a serem seguidas nesta abordagem, elucidando-as com alguns exemplos de parte de entrevistas realizadas numa pesquisa que teve como objetivos: compreender a experiência do idoso com a saúde bucal ao longo da vida e desenvolver e validar um modelo teórico representativo dessa experiência.

Estado neurológico e cardiorrespiratório de filhotes de cães nascidos de parto normal ou de cesariana sob anestesia geral inalatória com sevofluorano

A anestesia obstétrica possibilita um procedimento mais seguro para a mãe e para os fetos. em medicina veterinária, no entanto, a literatura científica a respeito do assunto é deficiente. Este trabalho teve como objetivo avaliar o grau de depressão neurológica, hemodinâmica e respiratória fetais provocado pelo agente anestésico, em que as mães foram submetidas ao parto normal ou à cesariana, utilizando-se sevofluorano como agente de manutenção anestésica, comparando-o com o parto normal. Foram realizados seis partos normais (GN) e seis cesarianas (GC), avaliando-se um total de 36 filhotes. As cesarianas foram realizadas utilizando-se acepromazina, propofol e sevofluorano (GC) e os neonatos foram avaliados clinicamente ao primeiro, quinto e décimo minuto de nascimento, nos dois grupos. Observou-se maior depressão respiratória nos filhotes nascidos de cesariana. Contudo, apesar dessa depressão, o protocolo anestésico empregado não comprometeu de maneira importante a viabilidade e a saúde das mães e dos filhotes, demonstrando ser seguro em cadelas gestantes.

Análise qualitativa do conteúdo ministrado na disciplina de bioética nas faculdades de odontologia brasileiras

The study consists on a qualitative analysis of content ministered by Bioethical discipline on Brazilian Dental Schools. Data collect occurred by semi-structured questioners send by e-mail and correspondence. It was used content analysis by categorical analysis technique, according to Bardin. Among 182 Schools, just 57 (31.3%) shows Bioethical Discipline. It was observed that the discipline is ministered on the first or last year of course generally. Principal subjects broached are bioethical dilemma (76.3%) with polemics questions and others actual like abortion, clone, organs transplant, attendance of patient porter of HIV and bioethical historic (65.8%), bioethical fundaments, principles and models (57.9%). 84.4% researched professors, said that bioethical discipline awake interesting on students because it actual and it's face to patient-professional relation too. It was possible to conclude that bioethical discipline treat in its content some actual and polemic themes that influence dental surgeon formation because promote him conscience and responsibility of him role front attention at oral health, and so, this discipline should be implanted, specifically in the first year of all Dentistry graduation courses.