Chemical composition and phytoplankton characteristics of water samples obtained during POLARSTERN cruise ANT-XXIV/3

The fixation of dissolved inorganic carbon (DIC) by marine phytoplankton provides an important feedback mechanism on concentrations of CO2 in the atmosphere. As a consequence it is important to determine whether oceanic primary productivity is susceptible to changing atmospheric CO2 levels Among numerous other factors, the acquisition of DIC by microalgae particularly in the polar seas is projected to have a significant effect on future phytoplanktonic production and hence atmospheric CO2 concentrations. Using the isotopic disequilibrium technique the contribution of different carbon species (CO2 and bicarbonate) to the overall DIC uptake and the extent to which external Carbonic Anhydrase (eCA) plays a role in facilitating DIC uptake was estimated. Simultaneous uptake of CO2 and HCO3- was observed in all cases, but the proportions in which different DIC species contributed to carbon assimilation varied considerably between stations. Bicarbonate as well as CO2 could be the major DIC source for local phytoplankton assemblages. There was a positive correlation between the contribution of CO2 to total DIC uptake and ambient concentration of CO2 in seawater suggesting that Southern Ocean microalgae could increase the proportion of CO2 uptake under future high atmospheric CO2 levels. Results will be discussed in view of metabolic costs related to DIC acquisition of Southern Ocean phytoplankton.

IMMUNE-ANGIOGENESIS MECHANISMS ASSOCIATED WITH PORCINE PREGNANCY SUCCESS AND FAILURE

Spontaneous fetal loss (25-40%) leading to decrease in litter size is a significant concern to the pork industry. A deficit in the placental vasculature has emerged as one of the important factors associated with fetal loss. During early pig pregnancy, the endometrium becomes enriched with immune cells recruited by conceptus-derived signals including specific chemokine stimuli. These immune cells assist in various aspects of placental development and angiogenesis. Recent evidence suggests that microRNAs (miRNAs: small non-coding RNAs that regulate gene expression) regulate immune cell development and their functions. In addition, intercellular communication including exchange of biomolecules (e.g. miRNAs) between the conceptus and endometrium regulate key developmental processes during pregnancy. To understand the biological significance of immune cell enrichment, regulation of their functions by miRNAs and transfer of miRNAs across the maternal fetal-interface, we screened specific sets of chemokines and pro- and anti-angiogenic miRNAs in endometrial lymphocytes (ENDO LY), endometrium, and chorioallantoic membrane (CAM) isolated from conceptus attachment sites (CAS) during early, gestation day (gd)20 and mid-pregnancy (gd50). We report increased expression of selected chemokines including CXCR3 and CCR5 in ENDO LY and CXCL10, CXCR3, CCL5, CCR5 in endometrium associated with arresting CAS at gd20. Some of these differences were also noted at the protein level (CXCL10, CXCR3, CCL5, and CCR5) in endometrium and CAM. We report for the first time significant differences for miRNAs involved in immune cell-derived angiogenesis (miR-296-5P, miR-150, miR-17P-5P, miR-18a, and miR-19a) between ENDO LY associated with healthy and arresting CAS. Significant differences were also found in endometrium and CAM for some miRNAs (miR-17-5P, miR-18a, miR-15b-5P, and miR-222). Finally, we confirm that placenta specific-exosomes contain proteins and 14 select miRNAs including miR-126-5P, miR-296-5P, miR-16, and miR-17-5P that are of relevance to early implantation events. We further demonstrated the bidirectional exosome shuttling between porcine trophectoderm cells (PTr2) and porcine aortic endothelial cells (PAOEC). PTr2-derived exosomes were able to modulate the endothelial cell proliferation that is crucial for the establishment of pregnancy. Our data unravels the selected chemokines and miRNAs associated with immune cell-regulated angiogenesis and reconfirm that exosome mediated cell-cell communication opens-up new avenues to understand porcine pregnancy.

Systematic review of Late Jurassic sauropods from the Museu Geológico collections (Lisboa, Portugal)

The Museu Geológico collections house some of the first sauropod references of the Lusitanian Basin Upper Jurassic record, including the Lourinhasaurus alenquerensis and Lusotitan atalaiensis lectotypes, previously considered as new species of the Apatosaurus and Brachiosaurus genera, respectively. Several fragmentary specimens have been classical referred to those taxa, but the most part of these systematic attributions are not supported herein, excluding a caudal vertebra from Maceira (MG 8804) considered as cf. Lusotitan atalaiensis. From the material housed in the Museu Geológico were identified basal eusauropods (indeterminate eusauropods and turiasaurs) and neosauropods (indeterminate neosauropods, diplodods and camarasaurids and basal titanosauriforms). Middle caudal vertebrae with lateral fossae, ventral hollow border by pronounced ventrolateral crests and quadrangular cross-section suggest for the presence of diplodocine diplodocids in north area of the Lusitanian Basin Central Sector during the Late Jurassic. A humerus collected from Praia dos Frades (MG 4976) is attributed to cf. Duriatitan humerocristatus suggesting the presence of shared sauropod forms between the Portugal and United Kingdom during the Late Jurassic. Duriatitan is an indeterminate member of Eusauropoda and the discovery of new material in both territories is necessary to confirm this systematic approach. The studied material is in according with the previous recorded paleobiodiversity for the sauropod clade during the Portuguese Late Jurassic, which includes basal eusauropods (including turiasaurs), diplodocids and macronarians (including camarasaurids and basal titanosauriforms).

Vermiculitas tratadas quimicamente na obtenção de sólidos microporosos como precursores para híbridos inorgânico-orgânicos com aplicações adsortivas

ALVES, Ana paula Melo. Vermiculitas tratadas quimicamente na obtenção de sólidos microporosos como precursores para híbridos inorgânico-orgânicos com aplicações adsortivas. 2009. 124 f. Tese (Doutorado em Quimica) - Centro de Ciências Exatas e da Natureza, Universidade Federal da Paraíba, João Pessoa, PB, 2009.

PERSONALIDADE E AGRESSIVIDADE EM ADOLESCENTES E JOVENS ADULTOS RESIDENTES EM BAIRROS SOCIAIS

Alguns estudos foram feitos no sentido de verificar a relação entre os traços de personalidade e a agressividade (e.g.Tyrode & Bourcet, 2002). Jean Pinatel (1987) foi um dos autores que definiu traços comuns de personalidade que se encontram frequentemente nos indivíduos delinquentes. Tais traços são: o egocentrismo, a labilidade, a agressividade e a indiferença afectiva. Contestando esta noção dos traços de personalidade, outros autores (e. g. Gibert & Daffer , 2011) defendem que o comportamento agressivo é entendido como resultado de uma série de experiências que levam o indivíduo a comportar-se de forma agressiva em situações diferentes. Esta investigação teve como objectivo estudar a relação entre a personalidade e a agressividade numa amostra de adolescentes e jovens adultos que moram em alguns bairros sociais da área de Lisboa, bem como contribuir para uma maior compreensão destas variáveis, nomeadamente a sua relação com as variáveis género, idade e escolaridade. Assim, a amostra foi constituída por 150 participantes, de ambos os sexos, com idades compreendidas entre os 15 e os 29 anos. Para avaliar as variáveis foram utilizadas as seguintes escalas: Big Five Inventory (BFI; Benet-Martínez & John, 1998) para medir a Personalidade e Aggression Questionnaire (AQ; Buss & Perry, 1992) para a Agressividade. Tendo em conta os principais dados teóricos sobre essas variáveis, espera-se que: 1) Os traços de personalidade dos adolescentes e jovens adultos se correlacionem de forma significativa com a agressividade; 2) Os participantes do sexo masculino apresentem valores diferentes nos traços de personalidade e na agressividade relativamente aos participantes do sexo feminino; 3) A idade se correlacione de forma significativa com as dimensões da personalidade e da agressividade nos adolescentes e jovens adultos; 4) A escolaridade se correlacione significativamente com as dimensões da personalidade e da agressividade nos adolescentes e jovens adultos. Concluiu-se que a Agradabilidade se correlaciona significativamente com a Agressividade Física e com a Agressividade Total, indicando que quanto maior a Agradabilidade menor a agressividade. Verificou-se que os rapazes e as raparigas apresentaram maiores diferenças na Agradabilidade e na Agressividade Física. A idade não apresentou correlações significativas com a agressividade, no entanto correlacionou-se de forma significativa com a Conscienciosidade e a Abertura à Experiência. E a escolaridade, por sua vez, apresentou correlações significativas com a Abertura à experiência, a Agressividade Física, a Hostilidade e a Agressividade Total.

O Papel das Memórias Positivas Precoces nos Estilos de Vinculação e Estados Emocionais Negativos dos Adolescentes

Objetivos: A literatura científica tem mostrado que as experiências precoces na infância, nomeadamente as relacionadas com sentimentos de ameaça ou de segurança desempenham um papel fundamental no desenvolvimento emocional e social posterior. O presente estudo apresenta os seguintes objetivos: 1) Adequar e analisar as propriedades psicométricas da escala de memórias precoces de calor e segurança (EMWSS) à população de adolescentes, garantindo, uma medida confiável do constructo em estudo; 2) Examinar o impacto das memórias precoces de calor e segurança nos estilos de vinculação; 3) Explorar a relação entre as memórias positivas precoces e indicadores de desajustamento psicológico (sintomas de depressão, ansiedade e stress). Método: A amostra é constituída por 651 adolescentes (330 rapazes e 321 raparigas) com idades compreendidas entre os 12 e os 19 anos a frequentarem o ensino básico e secundário do ensino regular. Foram completados 3 instrumentos de auto - resposta que avaliaram as memórias precoces de calor e segurança (EMWSS), vinculação (AQ) e sintomas de ansiedade, depressão e stress (DASS-21). Resultados: A EMWSS evidenciou uma boa consistência interna, uma estrutura fatorial unidimensional, uma excelente estabilidade temporal e uma boa validade discriminante. As memórias precoces de calor e segurança mostraram uma correlação moderada negativa com os sintomas de ansiedade, depressão e stress e uma adequada validade discriminante relativamente à qualidade de vinculação. Os adolescentes classificados com o estilo de vinculação segura evidenciaram significativamente mais memórias positivas de infância, que os jovens com um estilo de vinculação insegura (ambivalente ou evitamento). Conclusões: À semelhança dos estudos realizados com adultos, a EMWSS parece ser uma escala útil na prática clínica com adolescentes, revelando excelentes características Memórias Positivas Precoces, Estilos de Vinculação e Estados Emocionais Negativos 6 psicométricas. Este estudo mostrou igualmente como as memórias emocionais precoces (de calor e segurança) diferem em função da qualidade de vinculação (segura/insegura).

Validação da Escala de Memórias Precoces de Calor e Segurança em Relação ao Grupo de Pares (EMPCS-Pares)

A presente investigação pretendeu validar para a população portuguesa de adolescentes a Escala de Memórias Precoces de Calor e Segurança em Relação ao Grupo de Pares (EMPCSPares) bem como ver cumpridos os seus principais objetivos: 1) Adaptação da escala de memórias precoces de calor e segurança (EMPCS), enquanto medida global, para o contexto de interação com o grupo de pares; 2) Explorar a validade de construto através da análise fatorial exploratória (estudo da dimensionalidade); 3) Analisar a consistência interna do instrumento e explorar a qualidade dos itens; 4) Examinar a validade convergente e divergente através da associação com outras variáveis semelhantes e distintas do construto em análise; 5) Analisar possíveis efeitos das variáveis sociodemográficas, como a idade, género e escolaridade nos resultados da escala, bem como a sua associação com a perceção global de qualidade vida; 6) Comparar as memórias precoces emocionais em função da qualidade de vinculação (segura e insegura). A amostra é constituída por 354 jovens (152 rapazes e 202 raparigas), com idades compreendidas entre os 12 e os 18 anos (M= 15,81; DP = 1,58) a frequentar o ensino básico e secundário do sistema regular de ensino público (7ºano de escolaridade ao 12º ano de escolaridade). Do protocolo constam os seguintes instrumentos: escala de memórias precoces de calor e segurança no contexto familiar (EMPCSFamília) e na interação com o grupo de pares (EMPCSPares); escala de auto-compaixão (SCS-A); escala de vergonha externa (OAS – A); questionário de vinculação (AQ-C); escala de ansiedade, depressão e stress (EADS-21). Os resultados obtidos mostram que a escala tem uma estrutura unidimensional, possui uma excelente consistência interna, uma estabilidade temporal adequada, assim como uma boa validade convergente e divergente. Revelou igualmente discriminar os jovens com uma vinculação segura dos que apresentam uma vinculação insegura. Apesar da necessidade de mais estudos, nomeadamente a realizar em amostras clínicas, a EMPCSPares mostrou ser um instrumento robusto e útil na avaliação de memórias emocionais no contexto de interação com os pares, constituindo um contributo relevante para a investigação e prática clínica com adolescentes. / This research intends to validate for the Portuguese population of adolescents the Early Memories Scale Heat and Safety Relative to Peer Group (EMPCSPares) and see fulfilled its main objectives: 1) The early warm and security memories scale adaptation (EMPCS), as a global measure for the interaction context with the peer group; 2) Explore the construct validity by exploratory factor analysis (study of dimensionality); 3) To analyze the internal consistency of the instrument and explore the quality of the items; 4) Examine the convergent and divergent validity by association with other similar and different variables of the construct in question; 5) Analyze possible effects of sociodemographic variables such as age, gender and education in the scale results, as well as its association with the global perception of quality of life; 6) compare the emotional early memories due to the link quality (secure and insecure). The sample consists of 354 children (152 boys and 202 girls), aged between 12 and 18 years (M = 15.81, SD = 1.58) attending basic and secondary education in the regular public education system (7th grade to 12th grade). The Protocol contains the following instruments: Scale of early heat and security memories in the family context (EMPCSFamily) and interaction with the peer group (EMPCSPairs) ; Self - compassion scale (SCS-A); external shame scale (OAS - A); linking questionnaire (AQ- C) ; scale of anxiety, stress and depression (EADS 21) . The results obtained show that the scale has a one-dimensional structure, has an excellent internal consistency, an adequate temporal stability as well as good convergent and divergent validity. It also showed discriminate young people with a secure attachment of those who have an insecure attachment. Despite the need for further studies, including the conduct of clinical samples, the EMPCSPares proved to be a robust and useful tool in the evaluation of emotional memories in the context of interaction with peers, constituting an important contribution to research and clinical practice with adolescents.

Qualitätssicherung an österreichischen Hochschulen. Eine Bestandsaufnahme. Bericht gemäß §28 HS-QSG zum Stand 2015

Die AQ Austria ist durch das Hochschul-Qualitätssicherungsgesetz (HS-QSG) beauftragt, alle drei Jahre einen Bericht zur Entwicklung der Qualitätssicherung an hochschulischen Bildungseinrichtungen zu erstellen. In diesem ersten Bericht werden Gemeinsamkeiten und Spezifika der drei Hochschulsektoren – öffentliche Universitäten, Privatuniversitäten und Fachhochschulen – bei der Gestaltung des internen Qualitätsmanagements sowie bei den Maßnahmen der Qualitätssicherung und –entwicklung aufgezeigt. Die aktuellen Themenbereiche und Fragestellungen sind in die nationalen Rahmenbedingungen eingebettet, innerhalb derer die österreichischen Hochschulen agieren und die die Qualitätssicherung an den österreichischen Hochschulen mitbestimmen.

Quality audit in the European higher education area. A comparison of approaches

The publication illustrates various approaches to auditing and reflects on their merit, as well as outlines the implementation of audits in different higher education systems across Europe. One focus is to show common aspects and apparent deviations concerning purpose and aim of the audit, national legislation, scope of the audit, external assessments and their effects. In addition it reflects on current and future challenges and developments. Contributions from twelve European quality assurance agencies provide an insight into their audit approaches. The publication is targeted at quality assurance agencies, higher education institutions and other stakeholders and aims to increase knowledge about different quality assurance procedures across borders. With contributions by: Kastelliz, Dietlinde; Müller Strassnig, Annina; Kohler, Alexander; Huertas, Esther; Adot, Esther; Perez de la Calle, Jose Antonio; Balboa, Esther; Danian, Rado Mircea; Sarbu, Oana; Pedersen, Lars; Moitus, Sirpa; Leetz, Friederike; Froestad, Wenche; van Galen, Stephan; Le Fort, Genevieve; McLaughlin, Maureen; Crum, Ailsa.

Analysis of surfaces for characterization of fungal burden: does it matter?

Objectives: Mycological contamination of occupational environments can be a result of fungal spores’ dispersion in the air and on surfaces. Therefore, it is very important to assess it in both types of the samples. In the present study we assessed fungal contamination in the air and in the surface samples to show relevance of surfaces sampling in complementing the results obtained in the air samples. Material and Methods: In total, 42 settings were assessed by the analysis of air and surfaces samples. The settings were divided into settings with a high fungal load (7 poultry farms and 7 pig farms, 3 cork industries, 3 waste management plants, 2 wastewater treatment plants and 1 horse stable) and a low fungal load (10 hospital canteens, 8 college canteens and 1 maternity hospital). In addition to culture-based methods, molecular tools were also applied to detect fungal burden in the settings with a higher fungal load. Results: From the 218 sampling sites, 140 (64.2%) presented different species in the examined surfaces when compared with the species identified in the air. A positive association in the high fungal load settings was found between the presence of different species in the air and surfaces. Wastewater treatment plants constituted the setting with the highest number of different species between the air and surface. Conclusions: We observed that surfaces sampling and application of molecular tools showed the same efficacy of species detection in high fungal load settings, corroborating the fact that surface sampling is crucial for a correct and complete analysis of occupational scenarios.

Occupational exposure to fungi and particles in animal feed industry

Background: Very few studies regarding fungal and particulate matter (PM) exposure in feed industry have been reported, although such contaminants are likely to be a significant contributing factor to several symptoms reported among workers. The purpose of this study has been to characterize fungal and dust exposure in one Portuguese feed industry. Material and Methods: Air and surface samples were collected and subject to further macro- and microscopic observations. In addition we collected other air samples in order to perform real-time quantitative polymerase chain reaction (PCR) amplification of genes from Aspergillus fumigatus and Aspergillus flavus complexes as well as Stachybotrys chartarum. Additionally, two exposure metrics were considered – particle mass concentration (PMC), measured in 5 different sizes (PM0.5, PM1, PM2.5, PM5, PM10), and particle number concentration (PNC) based on results given in 6 different sizes in terms of diameter (0.3 μm, 0.5 μm, 1 μm, 2.5 μm, 5 μm and 10 μm). Results: Species from the Aspergillus fumigatus complex were the most abundant in air (46.6%) and in surfaces, Penicillium genus was the most frequently found (32%). The only DNA was detected from A. fumigatus complex. The most prevalent in dust samples were smaller particles which may reach deep into the respiratory system and trigger not only local effects but also the systemic ones. Conclusions: Future research work must be developed aiming at assessing the real health effects of these co-exposures.

Assessment of toxigenic fungi in poultry feed

Introduction - Feed supplies the necessary nutrients for the growth of healthy animals, which are a part of the human diet. The presence of toxigenic fungi in animal feed such as Aspergillus spp. may contribute to 1) the loss of nutritional value of feedstuff, since fungi will assimilate the most readily available nutrients present in the feed, and 2) the development of mycotoxicoses and chronic conditions, which can raise economic issues due to animal disease and contamination of animal derived products. Aim of the study - The goal of this work was to evaluate the incidence of Aspergilli, particularly from the Circumdati, Flavi and Fumigati sections, through real-time quantitative PCR (qPCR) in 11 feed samples.

Feed production, swine and slaughterhouse: where is the highest occupational exposure to Aspergillus spp. in this production line?

Purpose - This study intended to characterize fungal contamination in two swine farms, in one feed production unit, and also in one swine slaughterhouse. We aimed to identify where the highest occupational exposure to Aspergillus spp. was detected during the production line.

Fungi distribution in poultry feed

Feed can easily be contaminated and colonized by fungi that use up the nutrients for their own metabolism and growth, producing secondary metabolites such as mycotoxins that are not eliminated throughout the feed processing. The major problems associated with mycotoxin contaminated animal feed are metabolic disturbances resulting in poor animal productivity. In addition, handling contaminated animal feed can also raise health issues regarding workers exposure to fungi and mycotoxins. The scope of this work was to characterize fungal distribution in 11 poultry feed samples. Twenty grams of feed were suspended in 180 mL of distilled water and homogenized during 20 minutes at 200 rpm. The washed supernatant was plated in malt extract agar (MEA) and dichloran glycerol agar base (DG18) media for morphological identification of the mycobiota present. Using macro- and microscopic analysis of the colonies, fungal contamination was evident in 72.7% of the analyzed poultry feed samples. Fungal load ranged from 0 to 13140 CFU/g, and the most prevalent species/genera were F. graminearum complex (71.1%), Penicillium sp. (11.6%), Cladosporium sp. (8.8%), and Fusarium poae (3.6%). In addition to these species, we also isolated Aspergillus sections Circumdati, Nigri and Aspergilli, and Mucor and Rhizopus genus albeit at a lower abundance. The data obtained showed that, besides high fungal contamination, mycotoxins contamination is probably a reality, particularly in the final product since mycotoxins resist to all the processing operations including thermal treatment. Additionally, data claimed attention for the probable co-exposure to fungi and mycotoxins of the workers in feed industries.

Prevalence of Aspergillus section Fumigati in Portuguese slaughterhouses: a fungal and mycotoxin concern

Introduction - Within the Aspergillus genus, Aspergillus fumigatus species is one of the most ubiquitous saprophytic fungi and is considered the species with higher clinical relevance. The fungi belonging to the Fumigati section are the most common cause of invasive aspergillosis and a major source of infection related mortality in immunocompromised patients. One of the most abundant metabolites produced by Aspergillus fumigatus is the metabolite gliotoxin, which exhibits a diverse array of biologic effects on the immune system. Further, environments contaminated with A. fumigatus may be the cause or enhance respiratory problems in the workers of those specific settings. These species produce specific allergens and mycotoxins that could cause respiratory disorders. Aim of the study - The aim of the present work was to determine the prevalence of Aspergillus section Fumigati by cultural and molecular methods in poultry; swine and bovine; and large animal (bovine and horses) slaughterhouses.