Caracterização geotermobarométrica dos metabasitos de Cajamar (SP), Grupo São Roque, Cinturão Ribeira

Metamafic orthoderivate rocks, characterized by MORB signatures and paleoproterozoic ages occur in Cajamar region central east part of São Paulo State, Brazil. They are associated to philites, metasandstones and metalimestones of São Roque Group volcanosedimentary sequence, Southeastern portion of the Ribeira Belt. For the metamafic rocks the assemblage of metamorphic peak is represented by actinolitealbite- epidote-chlorite typical of regional metamorphism, greenschist facies. For a good characterization of P and T parameters of metamorphism microprobe analyses of main minerals were performed. Metamorphism did not destroy completely the primary mineralogy of original basalt and microgabbro, and augite crystals are registered in many samples, preserved together with metamorphic assemblage, and possibly indicate that the igneous temperatures of crystallization are between 900 and 1.100°C. Textures of mineral equilibrium for the peak paragenesis are not always present in the studied samples. It may be partially caused by the superposition of retrometamorphic events that is a consequence of regional shearing process. Where the equilibrium is reached, chiefly in the occurrences in the south of the area temperature and pressure determinations indicate values close to 487 ± 42°C and 8,2 ± 2.0 kbar, respectively. Other values for P and T lower than that of the peak were also obtained in central and north portion of the area and they are: 450 ± 68°C for T e 4.7 kbar for P and 315 ± 26°C for T and 3.0 kbar for P and may indicate that the mineral equilibrium was not reached in these samples or that they represent the retrometamorphic event. As a concluding remark considering the metamorphic P-T path now obtained one can say that the exhumation process in this area occurred by isothermal decompression.

Parâmetros físicos pré-eruptivos do magmatismo ácido da província magmática do Paraná: Resultados preliminares

A set of 12 samples of acid rock types Palmas (ATP) and Chapecó (ATC) was used to determine the chemical composition of plagioclase and pyroxene by electron microprobe, with the purpose to get information about the pressure and temperature of crystallization of these rocks. The results show that the pyroxene of ATP rocks (3,2 ± 1,2 kbar, max = 5,1 kbar and 1028 ± 38°C) were formed under pressure conditions higher than those ATC (1,8 ± 0,9 kbar, max = 3,4 kbar and 995 ± 26oC). However, the pressures obtained from plagioclase showed higher pressures for ATC (3.2 ± 1 kbar, max = 6,4 kbar and 1033 ± 12°C) than ATP (1,9 ± 1 kbar, max = 4,8 kbar and 1043 ± 5°C), suggesting that the crystallization of rocktype ATP began with the formation of pyroxene and plagioclase almost simultaneously at a depth of around 17 km while the ATC, began with the crystallization of plagioclase at a depth of about 21 km (assuming a gradient of 3,3 kbar/km). The geothermometry of plagioclase allow us to calculate the concentration of water from about 1 ± 0,3% H2O for both acid rock types. Additional calculations allow us to get the depth of water exsolution of magmatic liquid at 30m below the surface. Although the data are still preliminary and insufficient to model the extrusion of these rocks, they point out to an effusion mechanism of a partially fluidized magma by volatile, which would spread to large areas with small friction with the surface that would increased with the increase of viscosity caused by the loss of volatile and decreasing of temperature, developing coherent structures as lava flows.

Evolução do processo de espermiação em machos de Piau, Leporinus macrocephalus, hormonalmente induzidos à reprodução

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Transmissão do vírus da leprose dos citros por Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) para plantas associadas a pomares cítricos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Química mineral do vulcano-plutonismo paleoproterozoico da região de São Félix do Xingu (PA), Cráton Amazônico

As formações Sobreiro e Santa Rosa são resultado de intensas atividades vulcânicas paleoproterozoicas na região de São Félix do Xingu (PA), SE do Cráton Amazônico. A Formação Sobreiro é composta por rochas de fácies de fluxo de lava andesítica, com dacito e riodacito subordinados, além de rochas que compõem a fácies vulcanoclástica, caracterizadas por tufo, lapilli-tufo e brecha polimítica maciça. Essas rochas exibem fenocristais de clinopiroxênio, anfibólio e plagioclásio em uma matriz microlítica ou traquítica. O clinopiroxênio é classificado predominantemente como augita, com diopsídio subordinado, e apresenta caracterísiticas geoquímicas de minerais gerados em rochas de arco magmático. O anfibólio, representado pela magnesiohastingsita, foi formado sob condições oxidantes e apresenta texturas de desequilíbrio, como bordas de oxidação vinculadas à degaseificação por alívio de pressão. As rochas da Formação Santa Rosa foram extravasadas em grandes fissuras crustais de direção NE-SW, têm características de evolução polifásica e compõem uma fácies de fluxo de lava riolítica e riodacítica e uma fácies vulcanoclástica de ignimbritos, lapilli-tufos, tufos de cristais félsicos e brechas polimíticas maciças. Diques métricos e stocks de pórfiros graníticos e granitoides equigranulares completam essa suíte. Fenocristais de feldspato potássico, plagioclásio e quartzo dispersos em matriz de quartzo e feldspato potássico intercrescidos ocorrem nessas rochas. Por meio de análises químicas pontuais dos fenocristais em microssonda eletrônica, foram estimadas as condições de pressão e temperatura de sua formação, sendo que o clinopiroxênio das rochas intermediárias da Formação Sobreiro indica profundidade de formação variável entre 58 e 17,5 km (17,5 - 4,5 kbar), a temperaturas entre 1.294 e 1.082 ºC, enquanto o anfibólio cristalizou-se entre 28 e 15 km (7,8 - 4,1 kbar), o que sugere uma evolução polibárica. Assim, propõe-se um modelo de geração de magma basáltico hidratado com base na fusão parcial de cunha mantélica e no acúmulo na crosta inferior em uma zona quente, a partir da qual os magmas andesíticos e dacíticos são formados pela assimilação de crosta continental e cristalização fracionada.

Mapeamento geológico, caracterização litoquímica e potencial econômico do corpo de cromitito de Cedrolina, Santa Terezinha de Goiás - GO

Studies of mafic-ultramafic bodies have been carried out through the years due to their great use on the interpretation of geochemical and geotectonic processes that took place in Earth's history. Amongst them, chromitites are notably recognized for being excellent indicators of their parental magma chemistry and of different geotectonic environments, as well for frequently containing associated noble metals mineralization. Thus the investigation of one of this ultramafic bodies that occurs inside the Pilar de Goiás Greenstone Belt was proposed, resulting in a detailed map of the chromitites and country rocks, as well as innumerous new data on the chemistry of chromite and associated matrix and accessory minerals. These studies were based upon geological field observations, optic and Scanning Electron Microscope (SEM), besides electron microprobe and cathodoluminescence analysis performed at the “Eugen F. Stumpfl Laboratory” of the Montanuniversität Institute of Resource Mineralogy, University of Leoben - Leoben, Austria. The chromitites are composed of 40-70% in volume of chromite (~50% on average), 14-55% of talc (~30% on average), 3-60% of chromium rich chlorite (~20% on average), traces to 4% of iron hydroxides and traces to 3% of rutile (1,5% on average). The chromite occurs as large spherical aggregates or as fine grained subhedral crystals disseminated in the matrix. This aggregates have diameters of 0.3-1.5 cm (1 cm on average) and are extremely well rounded, massive to intensively fractured, and commonly deformed to ellipsoids. When observed under the microscope, these aggregates show well rounded to slightly irregular borders, but on their interiors, these structures are represented by fine to medium grained euhedral to subhedral chromite crystals that have sharp contacts between themselves. The rock's matrix is basically made of chlorite and talc that define a metamorphic foliation (Sn), being the talc an alteration product ...

Análises isoenzimáticas, moleculares e ultraestruturais em camarões dulcícolas do gênero Macrobrachium

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of ion irradiation on the structural properties and hardness of a-C:H:Si:O:F films

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detailed morphological description of Habronema clarki Foster &Chitwood, 1937, a nematode parasite of capybaras Hydrochoerus hydrochaeris (Linnaeus, 1766) in Brazil

The genus Habronema has four valid species, of which only two are properly known. The present study aimed to describe in detail the morphology of Habronema clarki through optical and scanning electron microscopy analyses. Our results showed that the labial morphology of this parasite is closer to H. muscae than to H. microstoma. Even so, the characteristic pseudolabia and the slightly convex border of the dorsal and ventral lips are sufficient to distinguish these nematodes. Additional morphological data are presented, thus contributing to the knowledge on this little known nematode. In addition, this study provides new locality records for this species.

In vitro organogenesis of zucchini squash cv. Caserta

A protocol for the in vitro culture of Cucurbita pepo cv. Caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. First, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in MS basal medium supplemented with benzylaminopurine (BAP, 4.5 mu M), under a 16-h photoperiod at 27 degrees C. Based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in MS basal medium supplemented with different concentrations of BAP (0, 1.1, 2.2, 3.3, 4.5, or 5.5 mu M) and incubated under a 16-h photoperiod at 27 degrees C. In vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium supplemented with 4.5 mu M of BAP. After 4 weeks of incubation the development of adventitious buds at the cotyledon/hypocotyl junction could be observed. These buds were transferred to elongation and rooting medium and the developed plants were acclimatized to greenhouse conditions. The morphogenic process was characterized using light and scanning electron microscopy analyses to confirm the organogenesis. The results showed that this alternate explant is efficient for in vitro culture of zucchini squash cv. Caserta. The protocol will be further examined for future use in genetic transformation experiments in this species.

Macroscopic and microscopic analysis of the tongue of the common opossum (Didelphis marsupialis)

We performed a macroscopic and microscopic study of the tongues of common opossums, Didelphis marsupialis, from South America. We studied two males and two females. We collected morphometric data on the tongue with precision calipers. For the light microscopy and scanning electron microscopy analyses, we fixed tissue fragments in 10% formaldehyde and 2.5% glutaraldehyde, respectively. The opossum tongues averaged 5.87 +/- 0.20 cm in length, 3.27 +/- 0.15 cm in width at the lingual body, and 3.82 +/- 0.15 cm in width at the root. The mean thickness of the lingual body was 1.8 +/- 0.1 cm, and the thickness of the root was 3.82 +/- 0.15 cm. Sharp filiform papillae were scattered across the entire tongue; conical filiform papillae occurred on the lingual body and tongue tip; fungiform papillae were scattered among the filiform papillae on the lingual body and tongue tip; and there were three vallate papillae at the root of the tongue. We found two strands of papillary projections in the tongue root. Despite the low variability observed in the lingual papillae, the morphological data obtained in this study may be related to the opossum's diverse food habits and the extensive geographic distribution of the species throughout America. Microsc. Res. Tech. 2012. (c) 2012 Wiley Periodicals, Inc.

Finite-strain analysis in orthogneiss of the Gran Paradiso massif, Western Alps, Italy

A finite-strain study in the Gran Paradiso massif of the Italian Western Alps has been carried out to elucidate whether ductile strain shows a relationship to nappe contacts and to shed light on the nature of the subhorizontal foliation typical of the gneiss nappes in the Alps. The Rf/_ and Fry methods used on feldspar porphyroclasts from 143 augengneiss and 11 conglomerate samples of the Gran Paradiso unit (upper tectonic unit of the Gran Paradiso massif), as well as, 9 augengneiss (Erfaulet granite) and 3 quartzite conglomerate samples from the underlying Erfaulet unit (lower unit of the Gran Paradiso massif), and 1 sample from mica schist. Microstructures and thermobarometric data show that feldspar ductility at temperatures >~450°C occurred only during high-pressure metamorphism, when the rocks were underplated beneath the overriding Adriatic plate. Therefore, the finite-strain data can be related to high-pressure metamorphism in the Alpine subduction zone. The augen gneiss was heterogeneously deformed and axial ratios of the strain ellipse in XZ sections range from 2.1 to 69.8. The long axes of the finite-strain ellipsoids trend W/WNW and the short axes are subvertical associated with a subhorizontal foliation. The strain magnitudes do not increase towards the nappe contacts. Geochemical work shows that the accumulation of finite strain was not associated with any significant volume strain. Hence, the data indicate flattening strain type in the Gran Paradiso unit and constrictional strain type in the Erfaulet unit and prove deviations from simple shear. In addition, electron microprobe work was undertaken to determine if the analysed fabrics formed during high-P metamorphism. The chemistry of phengites in the studied samples suggests that deformation and final structural juxtaposition of the Gran Paradiso unit against the Erfaulet took place during high-pressure metamorphism. On the other hand, nappe stacking occurred early during subduction probably by brittle imbrication and that ductile strain was superimposed on and modified the nappe structure during high-pressure underplating in the Alpine subduction zone. The accumulation of ductile strain during underplating was not by simple shear and involved a component of vertical shortening, which caused the subhorizontal foliation in the Gran Paradiso massif. It is concluded that this foliation formed during thrusting of the nappes onto each other suggesting that nappe stacking was associated with vertical shortening. The primary evidence for this interpretation is an attenuated metamorphic section with high-pressure metamorphic rocks of the Gran Paradiso unit juxtaposed against the Erfaulet unit. Therefore, the exhumation during high-pressure metamorphism in the Alpine subduction zone involved a component of vertical shortening, which is responsible for the subhorizontal foliation within the nappes.

Identification and dissolution behavior of the secondary uranium minerals in the corrosion products of Depleted Uranium (DU) ammunition formed in soils

In der vorliegenden Studie wurden verschiedene Techniken eingesetzt um drei Proben (4, 7, and 8) die aus denrnKorrosionsprodukten von aus dem Kosovo Krieg stammenden Munitionskugeln, bestehend aus abgereichertem Uranrn(Depleted Uranium - DU), zu untersuchen. Als erstes Verfahren wurde die Raman-Spektroskopie eingesetzt. Hierbeirnzeigte sichin den Proben, charakterisiert durch einen Doppelpeak, die Anwesenheit von Schoepitrn(UO2)8O2(OH)12(H2O)12. Der erste und zweite Peakzeigte sich im Spektralbereich von 840,3-842,5 cm-1rnbeziehungsweise 853,6-855,8 cm-1. Diese Werte stimmen mit den Literaturwerten für Raman-Peaks für Schoepitrnüberein. Des Weiteren wurde bei dieser Untersuchungsmethode Becquerelite Ca(UO2)6O4(OH)6(H2O)8 mit einemrnPeak im Bereich zwischen 829 to 836 cm-1 gefunden. Aufgrund des Fehlens des Becquerelitespektrums in derrnSpektralbibliothek wurde eine in der Natur vorkommende Variante analysiert und deren Peak bei 829 cm-1 bestimmt,rnwas mit den Ergebnissen in den Proben korrespondiert. Mittels Röntgenbeugung (X-Ray Diffraction, XRD) zeigtenrnsich in allen Proben ähnliche Spektren. Das lässt darauf schließen, dass das pulverisierte Material in allen Probenrndas gleiche ist. Hierbei zeigte sich eine sehr gute Übereinstimmung mit Schoepit und/oder meta-rnSchoepit(UO2)8O2(OH)12(H2O)10, sowie Becquerelite. Weiterhin war weder Autunit, Sabugalit noch Uranylphosphatrnanwesend, was die Ergebnisse einer anderen Studie, durchgeführt an denselben Proben, wiederlegt. DiernAnwesenheit von P, C oder Ca im Probenmaterial konnte ausgeschlossen werden. Im Falle von Calciumkann diesrnmit der Anwesenheit von Uran erklärt werden, welches aufgrund seines Atomradius bevorzugt in Becquerelite (1:6)rneingebaut wird. Die beiden Hauptpeaks für Uran lagen im Falle von U 4f 7/2 bei 382.0 eV und im Falle von U 4f 5/2rnbei 392 eV. Diese Werte mit den Literaturwerten für Schoepit und meta-Schoepitüberein. Die Ergebnissernelektronenmikroskopischen Untersuchung zeigen U, O, Ca, Ti als dominante Komponenten in allen Messungen.rnElemente wie Si, Al, Fe, S, Na, und C wurden ebenfalls detektiert; allerdings kann nicht ausgeschlossen werden,rndass diese Elemente aus dem Boden in der unmittelbaren Umgebung der Munitionsgeschosse stammen. Gold wurdernebenfalls gemessen, was aber auf die Goldarmierung in den Probenaufbereitungsbehältern zurückgeführt werdenrnkann. Die Elektronenmikroskopie zeigte außerdem einige Stellen in denen elementares Uran und Bodenmineralernsowie sekundäre Uranminerale auftraten. Die Elementübersicht zeigt einen direkten Zusammenhang zwischen U andrnCa und gleichzeitig keine Korrelation zwischen U und Si, oder Mg. Auf der anderen Seite zeigte sich aber einrnZusammenhang zwischen Si und Al da beide Konstituenten von Bodenmineralen darstellen. Eine mit Hilfe derrnElektronenstrahlmikroanalyse durchgeführte quantitative Analyse zeigte den Massenanteil von Uran bei ca. 78 - 80%,rnwas mit den 78,2% and 79,47% für Becquerelite beziehungsweise Schoepit aufgrund ihrer Summenformelrnkorrespondiert. Zusätzlich zeigt sich für Calcium ein Massenanteil von 2% was mit dem Wert in Becquerelite (2.19%)rnrecht gut übereinstimmt. Der Massenanteil von Ti lag in einigen Fällen bei 0,77%, was auf eine noch nicht korrodierternDU-Legierung zurückzuführen ist. Ein Lösungsexperiment wurde weiterhin durchgeführt, wobei eine 0,01 M NaClO4-rnLösung zum Einsatz kam in der die verbliebene Probensubstanz der Korrosionsprodukte gelöst wurde;rnNatriumperchlorate wurde hierbei genutzt um die Ionenstärke bei 0,01 zu halten. Um Verunreinigungen durchrnatmosphärisches CO2 zu vermeiden wurden die im Versuch für die drei Hauptproben genutzten 15 Probenbehälterrnmit Stickstoffgas gespült. Eine Modelkalkulation für den beschriebenen Versuchsaufbau wurde mit Visual MINTEQrnv.3.0 für die mittels vorgenannten Analysemethoden beschriebenen Mineralphasen im pH-Bereich von 6 – 10 imrnFalle von Becquerelite, und Schoepit berechnet. Die modellierten Lösungskurven wurden unter An- und Abwesenheitrnvon atmosphärischem CO2 kalkuliert. Nach dem Ende des Lösungsexperiments (Dauer ca. 6 Monate) zeigten diernKonzentrationen des gelösten Urans, gemessen mittels ICP-OES, gute Übereinstimmung mit den modelliertenrnSchoepit und Becquerelite Kurven. Auf Grund des ähnlichen Löslichkeitverhaltens war es nicht möglich zwichen denrnbeiden Mineralen zu unterscheiden. Schoepit kontrolliert im sauren Bereich die Löslichkeit des Urans, währendrnbecquerelit im basichen am wenigsten gelöst wird. Des Weiteren bleibt festzuhalten, dass ein Anteil an CO2 in diernverschlossenen Probenbehälter eingedrungen ist, was sich mit der Vorhersage der Modeldaten deckt. Die Löslichkeitrnvon Uran in der Lösung als Funktion des pH-Wertes zeigte die niedrigsten Konzentrationen im Falle einer Zunahmerndes pH-Wertes von 5 auf 7 (ungefähr 5,1 x 10-6 mol/l) und einer Zunahme des pH-Wertes auf 8 (ungefähr 1,5 x 10-6rnmol/l bei). Oberhalb dieses Bereichs resultiert jeder weitere Anstieg des pH-Wertes in einer Zunahme gelösten Uransrnin der Lösung. Der ph-Wert der Lösung wie auch deren pCO2-Wert kontrollieren hier die Menge des gelösten Urans.rnAuf der anderen Seite zeigten im Falle von Becquerelite die Ca-Konzentrationen höhere Werte als erwartet, wobeirnwahrscheinlich auf eine Vermischung der Proben mit Bodensubstanz zurückgeführt werden kann. Abschließendrnwurde, unter Berücksichtigung der oben genannten Ergebnisse, eine Fallstudie aus Basrah (Irak) diskutiert, wo inrnzwei militärischen Konflikten Uranmunition in zwei Regionen unter verschiedenen Umweltbedingungen eingesetztrnwurden.

Intraflagellar transport in the developing and mature vertebrate retina

Intraflagellar transport (IFT) is required for the assembly and maintenance of cilia. In this study we analyzed the subcellular localization of IFT proteins in retinal cells by correlative high-resolution immunofluorescence and immunoelectron microscopy. The rod photoreceptor cell was used as a model system to analyze protein distribution in cilia. To date the expression of IFT proteins has been described in the ciliary region without deciphering the precise spatial and temporal subcellular localization of IFT proteins, which was the focus of my work. rnThe establishment of the pre-embedding immunoelectron method was an important first step for the present doctoral thesis. Results of this work reveal the differential localization of IFT20, IFT52, IFT57, IFT88, IFT140 in sub-ciliary compartments and also their presence in non-ciliary compartments of retinal photoreceptor cells. Furthermore, the localization of IFT20, IFT52 and IFT57 in dendritic processes of non-ciliated neurons indicates that IFT protein complexes also operate in non-ciliated cells and may participate in intracellular vesicle trafficking in eukaryotic cells in general.rnIn addition, we have investigated the involvement of IFT proteins in the ciliogenesis of vertebrate photoreceptor cilia. Electron microscopy analyses revealed six morphologically distinct stages. The first stages are characterized by electron dense centriolar satellites and a ciliary vesicle, while the formation of a ciliary shaft and of the light sensitive outer segment disks are features of the later stages. IFT proteins were expressed during all stages of photoreceptor cell development and found to be associated with the ciliary apparatus. In addition to the centriole and basal body IFT proteins are present in the photoreceptor cytoplasm, associated with centriolar satellites, post-Golgi vesicles and with the ciliary vesicle. Therewith the data provide an evidence for the involvement of IFT proteins during ciliogenesis, including the formation of the ciliary vesicle and the elongation of the primary cilium of photoreceptor cells. Moreover, the cytoplasmic localization of IFT proteins in the absence of a ciliary shaft in early stages of ciliogenesis indicates roles of IFT proteins beyond their well-established function for IFT in mature cilia and flagella. rn

Dating brittle tectonic movements with cleft monazite: Fluid-rock interaction and formation of REE minerals

[1] Two millimeter-sized hydrothermal monazites from an open fissure (cleft) that developed late during a dextral transpressional deformation event in the Aar Massif, Switzerland, have been investigated using electron microprobe and ion probe. The monazites are characterized by high Th/U ratios typical of other hydrothermal monazites. Deformation events in the area have been subdivided into three phases: (D1) main thrusting including formation of a new schistosity, (D2) dextral transpression, and (D3) local crenulation including development of a new schistosity. The two younger deformational structures are related to a subvertically oriented intermediate stress axis, which is characteristic for strike slip deformation. The inferred stress environment is consistent with observed kinematics and the opening of such clefts. Therefore, the investigated monazite-bearing cleft formed at the end of D2 and/or D3, and during dextral movements along NNW dipping planes. Interaction of cleft-filling hydrothermal fluid with wall rock results in rare earth element (REE) mineral formation and alteration of the wall rock. The main newly formed REE minerals are Y-Si, Y-Nb-Ti minerals, and monazite. Despite these mineralogical changes, the bulk chemistry of the system remains constant and thus these mineralogical changes require redistribution of elements via a fluid over short distances (centimeter). Low-grade alteration enables local redistribution of REE, related to the stability of the accessory phases. This allows high precision isotope dating of cleft monazite. 232Th/208Pb ages are not affected by excess Pb and yield growth domain ages between 8.03 ± 0.22 and 6.25 ± 0.60 Ma. Monazite crystallization in brittle structures is coeval or younger than 8 Ma zircon fission track data and hence occurred below 280°C.