Comparison of experimental and numerical sloshing loads in partially filled tanks

Sloshing describes the movement of liquids inside partially filled tanks, generating dynamic loads on the tank structure. The resulting impact pressures are of great importance in assessing structural strength, and their correct evaluation still represents a challenge for the designer due to the high level of nonlinearities involved, with complex free surface deformations, violent impact phenomena and influence of air trapping. In the present paper, a set of two-dimensional cases, for which experimental results are available, is considered to assess the merits and shortcomings of different numerical methods for sloshing evaluation, namely two commercial RANS solvers (FLOW-3D and LS-DYNA), and two academic software (Smoothed Particle Hydrodynamics and RANS). Impact pressures at various critical locations and global moment induced by water motion in a partially filled rectangular tank, subject to a simple harmonic rolling motion, are evaluated and predictions are compared with experimental measurements. 2012 Copyright Taylor and Francis Group, LLC.

Numerical analysis of mixe-mode fracture of brickwork masonry with embedded discontinuity elements

One of the common pathologies of brickwork masonry structural elements and walls is the cracking associated with the differential settlements and/or excessive deflections of the slabs along the life of the structure. The scarce capacity of the masonry in order to accompany the structural elements that surround it, such as floors, beams or foundations, in their movements makes the brickwork masonry to be an element that frequently presents this kind of problem. This problem is a fracture problem, where the wall is cracked under mixed mode fracture: tensile and shear stresses combination, under static loading. Consequently, it is necessary to advance in the simulation and prediction of brickwork masonry mechanical behaviour under tensile and shear loading. The quasi-brittle behaviour of the brickwork masonry can be studied using the cohesive crack model whose application to other quasibrittle materials like concrete has traditionally provided very satisfactory results.

Simulation of Freight Trains Equipped with Partially Filled Tank Containers and Related Resonance Phenomenon

To study the fluid motion-vehicle dynamics interaction, a model of four, liquid filled two-axle container freight wagons was set up. The railway vehicle has been modelled as a multi-body system (MBS). To include fluid sloshing, an equivalent mechanical model has been developed and incorporated. The influence of several factors has been studied in computer simulations, such as track defects, curve negotiation, train velocity, wheel wear, liquid and solid wagonload, and container baffles. SIMPACK has been used for MBS analysis, and ANSYS for liquid sloshing modelling and equivalent mechanical systems validation. Acceleration and braking manoeuvres of the freight train set the liquid cargo into motion. This longitudinal sloshing motion of the fluid cargo inside the tanks initiated a swinging motion of some components of the coupling gear. The coupling gear consists of UIC standard traction hooks and coupling screws that are located between buffers. One of the coupling screws is placed in the traction hook of the opposite wagon thus joining the two wagons, whereas the unused coupling screw rests on a hanger. Simulation results showed that, for certain combinations of type of liquid, filling level and container dimensions, the liquid cargo could provoke an undesirable, although not hazardous, release of the unused coupling screw from its hanger. The coupling screw's release was especially obtained when a period of acceleration was followed by an abrupt braking manoeuvre at 1 m/s2. It was shown that a resonance effect between the liquid's oscillation and the coupling screw's rotary motion could be the reason for the coupling screw's undesired release. Possible solutions to avoid the phenomenon are given.Acceleration and braking manoeuvres of the freight train set the liquid cargo into motion. This longitudinal sloshing motion of the fluid cargo inside the tanks initiated a swinging motion of some components of the coupling gear. The coupling gear consists of UIC standard traction hooks and coupling screws that are located between buffers. One of the coupling screws is placed in the traction hook of the opposite wagon thus joining the two wagons, whereas the unused coupling screw rests on a hanger. This paper reports on a study of the fluid motion-train vehicle dynamics interaction. In the study, a model of four, liquid-filled two-axle container freight wagons was developed. The railway vehicle has been modeled as a multi-body system (MBS). To include fluid sloshing, an equivalent mechanical model has been developed and incorporated. The influence of several factors has been studied in computer simulations, such as track defects, curve negotiation, train velocity, wheel wear, liquid and solid wagonload, and container baffles. A simulation program was used for MBS analysis, and a finite element analysis program was used for liquid sloshing modeling and equivalent mechanical systems validation. Acceleration and braking maneuvers of the freight train set the liquid cargo into motion. This longitudinal sloshing motion of the fluid cargo inside the tanks initiated a swinging motion of some components of the coupling gear. Simulation results showed that, for certain combinations of type of liquid, filling level and container dimensions, the liquid cargo could provoke an undesirable, although not hazardous, release of an unused coupling screw from its hanger. It was shown that a resonance effect between the liquid's oscillation and the coupling screw's rotary motion could be the reason for the coupling screw's undesired release. Solutions are suggested to avoid the resonance problem, and directions for future research are given.

Experimental and Analytical Study of Masonry Infill Reinforced Concrete Frames Retrofitted with Steel Braces

In the present work a seismic retrofitting technique is proposed for masonry infilled reinforced concrete frames based on the replacement of infill panels by K-bracing with vertical shear link. The performance of this technique is evaluated through experimental tests. A simplified numerical model for structural damage evaluation is also formulated according to the notions and principles of continuum damage mechanics. The proposed model is calibrated with the experimental results. The experimental results have shown an excellent energy dissipation capacity with the proposed technique. Likewise, the numerical predictions with the proposed model are in good agreement with experimental results.

Low-cost retrofitting solutions for RC frames using masonry infill panels

A large number of reinforced concrete (RC) frame structures built in earthquake-prone areas such as Haiti are vulnerable to strong ground motions. Structures in developing countries need low-cost seismic retrofit solutions to reduce their vulnerability. This paper investigates the feasibility of using masonry infill walls to reduce deformations and damage caused by strong ground motions in brittle and weak RC frames designed only for gravity loads. A numerical experiment was conducted in which several idealized prototypes representing RC frame structures of school buildings damaged during the Port-au-Prince earthquake (Haiti, 2010) were strengthened by adding elements representing masonry infill walls arranged in different configurations. Each configuration was characterized by the ratio Rm of the area of walls in the direction of the ground motion (in plan) installed in each story to the total floor area. The numerical representations of these idealized RC frame structures with different values of Rm were (hypothetically) subjected to three major earthquakes with peak ground accelerations of approximately 0.5g. The results of the non-linear dynamic response analyses were summarized in tentative relationships between Rm and four parameters commonly used to characterize the seismic response of structures: interstory drift, Park and Ang indexes of damage, and total amount of energy dissipated by the main frame. It was found that Rm=4% is a reasonable minimum design value for seismic retrofitting purposes in cases in which available resources are not sufficient to afford conventional retrofit measures.

On soil-structure interaction in large non-slender partially buried structures

This paper addresses the seismic analysis of a deeply embedded non-slender structure hosting the pumping unit of a reservoir. The dynamic response in this type of problems is usually studied under the assumption of a perfectly rigid structure using a sub-structuring procedure (three-step solution) proposed specifically for this hypothesis. Such an approach enables a relatively simple assessment of the importance of some key factors influencing the structural response. In this work, the problem is also solved in a single step using a direct approach in which the structure and surrounding soil are modelled as a coupled system with its actual geometry and flexibility. Results indicate that, quite surprisingly, there are significant differences among prediction using both methods. Furthermore, neglecting the flexibility of the structure leads to a significant underestimation of the spectral accelerations at certain points of the structure.

A Damage Model for Masonry Infilled Frames

The effect of infill walls on the behaviour of frames is widely recognized, and, for several decades now, has been the subject of numerous experimental investigations. However, the analytical modeling of infilled panels and frames under in-plane loading is difficult and generally unreliable. From the point of view of the simulation technique the models may be divided into micromodels and simplified (or macro-) models. Based on the equivalent strut approach (simplified model), in this paper a damage model is proposed for the characterization of masonry walls submitted to lateral cyclic loads. The model, developed along the lines of the Continuum Damage Mechanics, have the advantages of including explicitly the coupling between damage and mechanical behaviour and so is consistent with the definition of damage as a phenomenon with mechanical consequences.

Performance of masonry buildings during the 2011 Lorca earthquake

On Wednesday 11th May 2011 at 6:47 pm (local time) a magnitude 5.1 Mw earthquake occurred 6 km northeast of Lorca with a depth of around 2 km. As a consequence of the shallow depth and the small epicentral distance, important damage was produced in several masonry constructions and even led to the collapse of some of them. Pieces of the facades of several buildings fell down onto the sidewalk, being one of the reasons for the killing of a total of 9 people. The objective of this paper is to describe and analyze the failure patterns observed in unreinforced masonry buildings ranging from 3 to 8 floors in height. First, a brief description of the local building practices of masonry buildings is given. Then, the most important failure types of masonry buildings are described and discussed. After that, a more detailed analysis of one particular building is presented.

Performance of buildings with masonry infill walls during the 2011 Lorca earthquake

On Wednesday 11th May 2011 at 6:47 pm (local time) a magnitude 5.1 Mw earthquake occurred 6 km northeast of Lorca with a depth of around 5 km. As a consequence of the shallow depth and the small epicentral distance, important damage was produced in several masonry constructions and even led to the collapse of one of them. Pieces of the facades of several buildings fell down onto the sidewalk, being one of the reasons for the killing of a total of 9 people. The objective of this paper is to describe and analyze the failure patterns observed in reinforced concrete frame buildings with masonry infill walls ranging from 3 to 8 floors in height. Structural as well as non-structural masonry walls suffered important damage that led to redistributions of forces causing in some cases the failure of columns. The importance of the interaction between the structural frames and the infill panels is analyzed by means of non-linear Finite Element Models. The resulting load levels are compared with the member capacities and the changes of the mechanical properties during the seismic event are described and discussed. In the light of the results obtained the observed failure patterns are explained. Some comments are stated concerning the adequacy of the numerical models that are usually used during the design phase for the seismic analysis.

Assessing construction and demolition waste on masonry works to avoid future generation

The difficulty of dealing with construction and demolition waste (CDW) on construction sites is not new and continues to be a significant environmental problem. Currently the CDW collection system in Spain is done in a decentralized manner by each sub-contracted company, being necessary to implement effective waste management measures ensuring a correct management and minimization. During the last years several measures have been launched in order to improve and encourage the reuse and recycling of CDW. A widespread solution for CDW recovery is using them as a landscaping aggregate or for road bases and sub-bases. However, measures encouraging onsite prevention still need to be enhanced. This paper studies the major work stage generating CDW and analyses the categories of CDW produced during its execution. For this, several real building sites have been analysed in order to quantify the estimation of CDW generated. Results of this study show that a significant contributor to the CDW generation on building construction sites in Spain are the masonry works. Finally, a Best Practices Manual (BPM) is proposed containing several strategies on masonry works aimed not only at CDW prevention, but also at improving their management and minimization. The use of this BPM together with the Study and Plan of CDW management --required by law--, promotes the environmental management of the company, favouring the cohesion of the construction process organization at all stages giving rise to establishing responsibilities in the field of waste and providing a greater control over the process. Keywords: construction and demolition waste, management, masonry works, good practice measures, prevention.

Hacia una taxonomía constructiva de las tapias de tierra y fábricas encofradas históricas = Towards a constructive taxonomy of the historical rammed-earth and formwork masonry

El objetivo fundamental de la investigación es el estudio de los fundamentos constructivos de las fábricas históricas de tapia de tierra y mampostería encofrada de las fortificaciones bajomedievales. Ante las incertidumbres detectadas en la datación de estas estructuras y frente al excesivo número de excepciones que quedan fuera de las clasificaciones tradicionalmente empleadas para estudiar este tipo de técnicas, el artículo desarrolla una propuesta de tipología o taxonomía constructiva basada en un sistema abierto. Este sistema nace del análisis de un número significativo de fábricas y atiende a diversos parámetros de control para la completa caracterización de cada fábrica: material, grado de compactación, función constructiva, encofrado, acabado superficial, combinación de materiales y situación, profundidad, formación y sección de los agujales. Cada fábrica se clasifica mediante una etiqueta alfanumérica. El sistema permite establecer tipos constructivos con los que se genera una clasificación cronotipológica constructiva. ABSTRACT The principal aim of the research is the study of the constructive reasons of the historical masonries built with rammed earth and formwork masonry, belonging to late medieval fortifications. Uncertainties have been warned about dating the historical masonries and there are excessive exceptions that do not fall in the rankings traditionally employed to study these techniques. Due to these reasons, the article develops a proposal of constructive typology or taxonomy based in an open system that comes from the analysis of a representative number of masonries and that attends to several control parameters tor the complete characterization of each masonry: material, compaction degree, constructive function, formwork, rendering, combination of materials and, last, the situation, deepness, formation and section of the putlogholes. Each masonry is classified through an alphanumeric label. The system allows establishing several constructive types which it is possible to do a cronotypological and constructive classification.

Juan Bautista Lázaro y la restauración monumental: su intervención en la Catedral de León (1892-1909)

La catedral de León, debido al estado de ruina que presentaba a mediados del siglo XIX, se vio sometida a partir de entonces a una serie de intervenciones de conservación, restauración y reconstrucción que la transforma-ron significativamente. A pesar de encontrarnos con gran cantidad de documentación escrita sobre este monumento y de existir varios estudios sobre los arquitectos restauradores que intervinieron en este a finales del siglo XIX, resulta sorprendente que todavía existan períodos de este lapso de tiempo en el que su análisis y profundización haya sido menor. Este es el caso del período de Juan Bautista Lázaro como arquitecto director de las obras de la catedral leonesa, existiendo únicamente sobre toda su obra un estudio general que fue realizado por el director de este trabajo de investigación, por lo que nos llevó a considerar que era oportuno profundizar sobre la figura y obra de uno de los arquitectos más importante de la restauración en España de este período, no solo por su obra en sí, sino también por sus pos-turas significativas respecto a la intervención en el patrimonio arquitectónico: su respeto por los añadidos históricos, su preferencia por no aislar los monumentos, sus interés por los sistemas constructivos y su deseo de recuperar los oficios tradicionales que estaban completamente perdidos en la España decimonónica. El objetivo principal de la tesis es, por tanto, el análisis descriptivo y arquitectónico de los proyectos e intervenciones que Lázaro desarrolló y ejecutó para la catedral de Santa María de Regla de León, para deducir los principios e ideas que guiaron su quehacer en este templo, y poder, tras estudiar sus diversas publicaciones escritas y los proyectos e intervenciones de restauración acometidas en otros edificios de carácter monumental, llegar a establecer sus criterios de intervención en el patrimonio histórico y artístico. De acuerdo con esto, la tesis se estructura en tres partes: una primera parte en la que se contempla el pensamiento arquitectónico de Lázaro en su intervención en el patrimonio histórico; una segunda, en la que se abordan directamente los proyectos e intervenciones de Lázaro en la pulchra leonina, previa aproximación del citado arquitecto al proceso de restauración que se acometía en dicho templo; y una tercera, en la que se realiza el estudio de las principales intervenciones de restauración que ejecutó en otros edificios del patrimonio arquitectónico español. El presente trabajo de investigación se ha servido de la documentación escrita, gráfica, planimétrica y fotográfica que sobre los temas tratados existe desperdigada en diversos archivos dependientes de distintos organismos oficiales civiles y eclesiásticos. Además, el hecho de haber podido acceder a los trabajos de conservación y restauración que en estos momentos se están llevando a cabo en las zonas en las que intervino Lázaro (vidrieras, rejas, puertas, fábricas, cubiertas, etc.), y el poder intercambiar opiniones y criterios con la persona que actualmente es responsable de las obras de la catedral de León, ha facilitado y complementado el desarrollo de la presente tesis al haber podido contrastar y tomar datos directamente en el monumento objeto de estudio. También se ha llevado a cabo la consulta de las distintas revistas de arquitectura publicadas en aquellos años y de los múltiples libros que versaban sobre los edificios objeto de este trabajo o que guardaban relación con ellos. Asimismo hemos logrado conocer el testimonio de algunos de los herederos de Lázaro y de varios de los descendientes de los operarios que trabajaron bajo sus órdenes. Como conclusiones generales, la tesis define por primera vez los criterios generales que guiaron su quehacer profesional en el patrimonio. Además, en este trabajo se analizan pormenorizadamente los proyectos y actuaciones de Lázaro en la catedral de León, abordándose también en profundidad el trabajo del mencionado arquitecto en el ámbito de la restauración arquitectónica. Para lograr este objetivo, esta tesis no se ha limitado únicamente a analizar la información existente en los proyectos y en los in-formes de supervisión de los mismos, como tradicionalmente se había hecho, sino que también se ha tratado de verificar si los datos contenidos en los diferentes documentos de un mismo proyecto y en los borradores de los mismos tenían relación y coherencia entre sí. Además, se ha procurado realizar el análisis comparativo de la realidad construida con el contenido de los proyectos, liquidaciones y documentos de la fase de ejecución de las obras. Este análisis comparativo no había sido realizado hasta la fecha, siendo este, por tanto, una de las aportaciones del trabajo. Por último, la tesis abre tres vías de investigación futuras (que ya se han tratado y avanzado en parte, pero que escapan a los límites de este trabajo). Estas se refieren al estudio de la evolución de la representación gráfica de los planos de la catedral leonesa catalogados en este trabajo, de la actividad pública de Lázaro y su preocupación por la defensa del patrimonio como diputado en las Cortes, arquitecto municipal de Ávila y arquitecto diocesano de Ávila y Toledo, y de cómo sus actuaciones de restauración en el patrimonio arquitectónico influyeron en los proyectos de obra nueva que construyó. ABSTRACT Due to the state of ruin in mid-nineteenth century, the Cathedral of Santa Maria de Regla of León was subjected to a series of interventions on conservation, restoration and reconstruction that transformed it significantly. There have been several studies on the architects who took part in the restoration during the mentioned century and despite abundant documentation and research on this monument, it is surprising to note the lack of deep analysis in some of the periods. In particular, the interventions accomplished by Juan Bautista Lázaro, as chief architect of the works of the Cathedral of León, have not been documented and analysed in adequate depth, despite the outstanding recognition of his work nationally and internationally. Furthermore, up to date, only the director of this research had conducted a general research of all his architectural work. For these reasons, we felt it was appropriate to elaborate on the life and work of one of the most important architects of the restoration in Spain during that period, not only for his work itself, but also for his significant contribution to the debate on architectural interventions on heritage. He is notable for his respect to historical additions, his preference for not isolating the monuments, his interest in building systems and his continuous desire to re-cover the traditional crafts that were completely lost in the nineteenth century in Spain. The main aim of this thesis is therefore the descriptive and architectural analysis of Lázaro’s projects and interventions developed and executed for the Cathedral of León. The thesis identifies the principles and ideas that guided his work in this temple and establish the criteria for intervention in the historical and artistic heritage architecture he applied after studying its various print publications, projects and restoration works undertaken in other buildings. Accordingly, the thesis is structured in three parts: the first part where it deals Lázaro’s architectural thinking in his interventions on heritage constructions; the second one, in which analyses the projects and interventions carried out by Lazaro in the “pulchra leonina”, but this analysis is preceded by the description of Lázaro’s relationship with the Cathedral of León before taking charge of its restoration in 1892; and the third and final section, in which the study of major restoration interventions implemented by Lázaro in other buildings of Spanish architectural heritage is made. This research has used available documentation (in written or graphic form, plans or photographs) scattered in a diverse range of archives de-pendent on various civil and ecclesiastical institutions. Moreover, we could access the conservation and restoration works, currently being carried out in areas where Lázaro intervened (stained glass windows, forged iron en-closures, gates, doors, masonry, covers, etc.). It has been possible to ex-change views and opinions with the person currently in charge of the referred restoration works which has facilitated and complemented the development of this thesis. It further, it has allowed contrasting and obtaining data directly from the monument under study. Consultation of various architectural journals published in those years has also been taken into account, as well as a diversity of books and articles which concerned the buildings assessed in this work, or related to them. Also, although not with the results expected, it has been obtained the testimony of some of Lázaro’s descendants as well as descendants of the workers who operated under him. As a general conclusion, the thesis defines the general criteria that guided his professional work in the heritage for the first time. Furthermore, Juan Bautista Lázaro’s projects and interventions in the Cathedral of León are analysed in detail. Also, it has been studying in detail the Lazaro’s work in the architectural heritage. To achieve this goal, the thesis has studied not only the information from the official projects and technical reports of them, but also it has been tried to verify if the information contained in the different documents of the same project and in the drafts were related and consistent with each other. In addition, it has been attempted to per-form a comparative analysis of the execution of Lázaro’s projects and the entire projected content. This comparative study had not been done to date and this will be, therefore, one of the main contributions of this re-search. Finally, this thesis opens up three lines of investigation (that have already been discussed and partially advanced, but which fall beyond the scope of this research). These refer to the study of the evolution of the graphical representation of the plans of Leon Cathedral catalogued in this thesis, public activity of Lazaro and his concern for the defence of heritage as deputy in Parliament, municipal architect of Avila, and as diocesan architect Ávila and Toledo, and even more how its restoration actions in architectural heritage influenced his projects of new construction.

HLA class I and II antigens are partially co-clustered in the plasma membrane of human lymphoblastoid cells

Major histocompatibility complex (MHC) class II molecules displayed clustered patterns at the surfaces of T (HUT-102B2) and B (JY) lymphoma cells characterized by interreceptor distances in the micrometer range as detected by scanning force microscopy of immunogold-labeled antigens. Electron microscopy revealed that a fraction of the MHC class II molecules was also heteroclustered with MHC class I antigens at the same hierarchical level as described by the scanning force microscopy data, after specifically and sequentially labeling the antigens with 30- and 15-nm immunogold beads. On JY cells the estimated fraction of co-clustered HLA II was 0.61, whereas that of the HLA I was 0.24. Clusterization of the antigens was detected by the deviation of their spatial distribution from the Poissonian distribution representing the random case. Fluorescence resonance energy transfer measurements also confirmed partial co-clustering of the HLA class I and II molecules at another hierarchical level characterized by the 2- to 10-nm Förster distance range and providing fine details of the molecular organization of receptors. The larger-scale topological organization of the MHC class I and II antigens may reflect underlying membrane lipid domains and may fulfill significant functions in cell-to-cell contacts and signal transduction.

Structure and function in rhodopsin: Rhodopsin mutants with a neutral amino acid at E134 have a partially activated conformation in the dark state*

The Glu-134–Arg-135 residues in rhodopsin, located near the cytoplasmic end of the C helix, are involved in G protein binding, or activation, or both. Furthermore, the charge-neutralizing mutation Glu-134 to Gln-134 produces hyperactivity in the activated state and produces constitutive activity in opsin. The Glu/Asp-Arg charge pair is highly conserved in equivalent positions in other G protein-coupled receptors. To investigate the structural consequences of charge-neutralizing mutations at Glu-134 and Arg-135 in rhodopsin, single spin-labeled side chains were introduced at sites in the cytoplasmic domains of helices C (140), E (227), F (250), or G (316) to serve as “molecular sensors” of the local helix bundle conformation. In each of the spin-labeled rhodopsins, a Gln substitution was introduced at either Glu-134 or Arg-135, and the electron paramagnetic resonance spectrum of the spin label was used to monitor the structural response of the helix bundle. The results indicate that a Gln substitution at Glu-134 induces a photoactivated conformation around helices C and G even in the dark state, an observation of potential relevance to the hyperactivity and constitutive activity of the mutant. In contrast, little change is induced in helix F, which has been shown to undergo a dominant motion upon photoactivation. This result implies that the multiple helix motions accompanying photoactivation are not strongly coupled and can be induced to take place independently. Gln substitution at Arg-135 produces only minor structural changes in the dark- or light-activated conformation, suggesting that this residue is not a determinant of structure in the regions investigated, although it may be functionally important.

Partially Processed pre-rRNA Is Preserved in Association with Processing Components in Nucleolus-derived Foci during Mitosis

Previous studies showed that components implicated in pre-rRNA processing, including U3 small nucleolar (sno)RNA, fibrillarin, nucleolin, and proteins B23 and p52, accumulate in perichromosomal regions and in numerous mitotic cytoplasmic particles, termed nucleolus-derived foci (NDF) between early anaphase and late telophase. The latter structures were analyzed for the presence of pre-rRNA by fluorescence in situ hybridization using probes for segments of pre-rRNA with known half-lives. The NDF did not contain the short-lived 5′-external transcribed spacer (ETS) leader segment upstream from the primary processing site in 47S pre-rRNA. However, the NDF contained sequences from the 5′-ETS core, 18S, internal transcribed spacer 1 (ITS1), and 28S segments and also had detectable, but significantly reduced, levels of the 3′-ETS sequence. Northern analyses showed that in mitotic cells, the latter sequences were present predominantly in 45S-46S pre-rRNAs, indicating that high-molecular weight processing intermediates are preserved during mitosis. Two additional essential processing components were also found in the NDF: U8 snoRNA and hPop1 (a protein component of RNase MRP and RNase P). Thus, the NDF appear to be large complexes containing partially processed pre-rRNA associated with processing components in which processing has been significantly suppressed. The NDF may facilitate coordinated assembly of postmitotic nucleoli.