Going live: a comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites

Fluorescent proteins have proven to be important tools for in vitro live imaging of parasites and for imaging of parasites within the living host by intravital microscopy. We observed that a red fluorescent transgenic malaria parasite of rodents, Plasmodium berghei-RedStar, is suitable for in vitro live imaging experiments but bleaches rapidly upon illumination in intravital imaging experiments using mice. We have therefore generated two additional transgenic parasite lines expressing the novel red fluorescent proteins tdTomato and mCherry, which have been reported to be much more photostable than first- and second-generation red fluorescent proteins including RedStar. We have compared all three red fluorescent parasite lines for their use in in vitro live and intravital imaging of P. berghei blood and liver parasite stages, using both confocal and wide-field microscopy. While tdTomato bleached almost as rapidly as RedStar, mCherry showed improved photostability and was bright in all experiments performed.

A comparative analysis of policies and other factors which affect the role of the private sector in economic development /

"PN-AAL-031"--Cover.

A comparative analysis of results from three recent non-separated concurrent-flow high occupancy freeway lane projects : Boston, Santa Monica, and Miami.

"Service and methods demonstration program."

A comparative analysis of bicycle lanes versus wide curb lanes: final report.

Federal Highway Administration, Office of Safety and Traffic Operations, Washington, D.C.

Evaluation of seismometer wave gage and comparative analysis of wave data at Yaquina and Coquille Bays, Oregon /

"September 1985."

Dade County Trauma Task Force: a multi-system comparative analysis.

National Highway Traffic Safety Administration, Washington, D.C.

A Comparative analysis of bicycle lanes versus wide curb lanes : final report /

"October 1999."

A comparative analysis of urban transportation requirements, volume I. Final report.

Federal Highway Administration, Washington, D.C.

A comparative analysis of urban transportation requirements, volume II. Final report.

Federal Highway Administration, Washington, D.C.

A comparative analysis of information on national industrial R&D expenditures.

"March 1985"--P. iii.

A comparative analysis of regional blood supply systems /

Mode of access: Internet.

Health systems plans in Illinois : a comparative analysis /

Cover title: Hsp analysis: an analysis of health systems plans in Illinois.

The apostolical authority of the Epistle to the Hebrews: an inquiry, in which the received title of the Greek epistle is vindicated, against the cavils of objectors, ancient and modern, from Origen to Sir J. D. Michaëlis, chiefly upon grounds of internal evidence hitherto unnoticed: comprising a comparative analysis of the style and structure of this epistle, and of the undisputed epistles of St. Paul, tending to throw light upon their interpretation.

Mode of access: Internet.

A comparative analysis of the Bayes inventory policy

Mode of access: Internet.