A proporção de plasma rico em plaquetas/enxerto ósseo autógeno pode influenciar o reparo ósseo de defeito de tamanho crítico: estudo radiográfico em calvária de ratos

Introduction: It has been suggested that bone grafts combined with platelet rich plasma (PRP) present greater density than bone grafts without PRP after healing process. However, this result may depend on proportion PRP/autogenous bone (AB) graft used. Objective: The purpose of this study was to analyze radiographically the influence of the proportion PRP/particulate autogenous bone (AB) graft on bone healing in surgically created criticalsize defects (CSD) in rat calvaria. Material and method: 50 rats were divided into 5 groups: C, AB, AB/PRP-50, AB/PRP-100 and AB/PRP-150. A 5 mm diameter CSD was created in the calvarium of each animal. In Group C the defect was filled by blood clot only. In Group AB the defect was filled with 0.01 mL of AB graft. In groups AB/ RP-50, AB/PRP-100 and AB/PRP-150 the defects were filled with 0.01 mL of AB graft combined with 50, 100 and 150 µL of PRP, respectively. All animals were euthanized at 30 days post-operative. Standardized radiographic images of the rat calvaria were obtained using Digora System (Soredex, Finland). The images were evaluated by three examiners using scores that indicated the percentage of radiopacity of the defect. The obtained data were subjected to statistical analysis (Kruskal-Wallis test, p < 0.05). Result: Group C presented radiopacity significantly lower than groups AB, AB/PRP-50, AB/PRP-100 and AB/PRP-150. Group AB/PRP-50 showed radiopacity significantly greater than groups AB/PRP-100 and AB. Conclusion: It can be concluded that the proportion PRP/AB graft influences bone healing in CSD in rat calvaria.

Reconstrução de maxila severamente atrófica via enxerto ósseo autógeno de calota craniana: descrição de técnica

Bone reconstructions are traditionally conducted with autogenous grafts harvested from intra- or extra-oral donor sites to reestablish the lost bone volume for further implant-prosthetic rehabilitation. The calvarial bone has been studied as an excellent donor site in large atrophic situations, presenting low resorption rates, as well as complications and minimal morbidity. The hospitalization time is short, with low pain levels, short functional limitations, and invisible scars. The skull microarchitecture is predominantly cortical in the presence of growth factors that demonstrate their osteogenic, osteoinductive, and osteoconductive abilities resulting in low resorption rate and high predictability when compared to the iliac crest. Dural lacerations, extra and subdural bleeding, cerebrospinal fluid leakage, and brain damage have been minimized due to the development of surgical technique. The delimitation of diploe, preserving the internal skull cortex before osteotomy at the donor made it possible to reduce accidents and complications. The aim of this paper is to show a technical and to discuss aspects of the use of calvarial bone in the reconstruction of severely atrophic maxilla for oral rehabilitation with osseointegrated implants.

Avaliação da incorporação de enxerto osseo homogeno fresco congelado em humanos: análise histologica e imuno-histoquímica

Allogeneic, fresh-frozen bone has been used in order to replace bone autografts. However, its osteoinduction and osteoconduction properties are not well-defined in the scientific literature. This work aimed to evaluate samples of homogenous bone grafts in humans by qualitative histological and immunohistochemical analysis. For this, ten pre-selected patients underwent surgical augmentation of bone defects. The homogenous fresh frozen block bone graft was stabilized and fixed by bicortical screws. After six months, the reopening procedure was performed for installation of osseointegrated implants. At this time surgical bone graft samples were removed by means of drill trephine. The samples were fixed in 10% formalin, processed with decalcified paraffin, and stained with hematoxylin and eosin. Immunohistochemistry was performed for the expression of Caspase 3 enzyme. The slides were brought to light microscopy for qualitative histology and immunohistochemistry. The results showed non-vital bone tissue, with few areas of deposition of new bone formation on the amorphous matrix, presence of chronic inflammatory infiltrate with areas of osteomyelitis, and expressive immunolabeling of Caspase 3. Given the methods employed and the results it was concluded that the allograft fresh-frozen block is not incorporated into the recipient bed after a healing period of six months.

Enxerto ósseo em odontologia: revisão de literatura

Bone is a specialized connective tissue, vascular and dynamic changes over the life of the organism. When injured, has a unique ability to regenerate and repair without the presence of scars, but in some situations due to the size of the defect, the bone tissue does not regenerate so completely, it is necessary to carry out bone grafting procedures. Considering there are various types of grafts and various donor sites. Thus, the aim of this study was to review the literature to some type of graft most commonly used in dentistry. Given the importance of bone reconstruction in oral and maxillofacial surgery, it is necessary to know the viability and influence of biomaterials, or not associated with autogenous grafts in bone repair. Even this, with many qualities, but further studies should be done to achieve each day, a synthetic material compatible with bone tissue lost in adequate amounts without requiring extra-oral surgeries that are considered to be devoted to higher morbidity.

As representações sociais de crianças em acolhimento institucional: proteção ou revitimização?

Pós-graduação em Serviço Social - FCHS

Estudo do processo de reparo de enxerto ósseo em bloco submetido ao recobrimento com etil-cianoacrilato: análise histométrica e imunoistoquímica em ratos

Pós-graduação em Odontologia - FOA

O uso místico da jóia: amuletos de proteção infantil no século XVII

Since the beginning of time man has used body ornaments, often-simple element of decoration, passing by the object ritualistic and religious icon, also as a symbol to the demonstration of status and power. Many of these objects have survived to today, but the vast majority is lost in time or was dismantled to reuse the material in new jewelry. The history of jewelry uses the pieces that have survived intact, documents and images of those jewels. This work uses of Visual Arts, more specifically the portrait art of, to analyze the mystical amulets of child`s protection jewel in works of painters Juan Pantoja dela Cruz and Diego Velásquez.

Avaliação de métodos de proteção para taludes em estrada não-pavimentada

This work presents the results of the efficiency of three protection methods of roads slopes, against the development of erosion processes.The methods anlyzed were: (1) grass planting "San Carlos" (Axonopus compressus), (2) litter, composed of remains of branches and leaves of eucalyptus E. Grandis, and (3) Compacted soil cover with a local soil with properties more resistant to erosion processes.The experiment consisted of assembly of four rectangular cells with 6 meters, three for protection systems in study and one was kept unprotected to evaluate the efficiency of the methods in study.The determination of the amount of eroded sediment was based on the sum of the solids in suspension and stored sediments in the geotextile bags.The results showed that, compared to the unprotected cell, the litter obtained a reduction of soil loss of 90%, while the grass enveloped and reduced 80% and 65% respectively.

Direito à desconexão do trabalho como instrumento de proteção à saúde do trabalhador

Pós-graduação em Direito - FCHS

Papel de cepas de Streptococcus mutans e Bifidobacterium spp. na etiologia ou proteção contra doenças bucais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência da aplicação de laser de baixa intensidade no recobrimento radicular associado à técnica de enxerto de tecido conjuntivo em indivíduos fumantes: ensaio clínico controlado randomizado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise das Áreas de Proteção Permanente do perímetro urbano do município de Rio Claro, SP, visando uma proteção e revitalização por meio de um gerenciamento baseado no PMBOK

The constant misuse of land in Permanent Preservation Areas (APPs) have been recurrent, especially in urban areas, where the dynamics of change is constant. The protection of these areas to maintain a balance in the ecosystem and the population's quality of life is fundamentally important. So it is of utmost important to draw up a mechanism that can help in decision-making in relation to measures for the protection, mitigation of damages that are being caused and revitalization of these areas. Thus, a managing these areas based on the PMBOK (Project Management Body of Knowledge) in order to create mechanisms to facilitate the protection and revitalization of APPs, could be of great help for making public decision-making bodies that are entrusted to protect such areas. Based on this, we elaborated a project management in order to revitalize and protect the APPs in urban area of Rio Claro, SP and could become a model for future use by public bodies

Utilização de extratos vegetais em proteção de plantas

Usage of pesticies in excessive doses or in an inadequate way to control diseases, pests and weeds, has promoted significant environmental damage, contamination of animals, intoxication of farmers, among others. These factors lead to occur the resistance of weeds, pests and pathogens, the appearance of iatrogenic diseases, biological imbalance and reduction in biodiversity. Seeking less aggressive alternatives, plant extracts have been used successfully. Compared to synthetic products, they offer great advantages as generating new compounds, which pathogens are unable to inactivate, and are less toxic, of rapid degradation in the environment, they have broad modes of action, and are derived from renewable resources. It should be emphasized that there are some limitations on its use. The extracts have potencial as insecticide, fungicide, herbicide and nematicide and are considered of good efficiency. The methodologies for obtaining can be achieved through cold extraction, open and closed hot system. The use of plant extracts reappears as a promising and unique option for integrated management in plant protection, being considered as an alternative to control plant pathogens, demonstrating excellent results without causing harm to the environment and living beings.

Identificação de áreas degradadas por extração de argila no município de Ourinhos (SP): recuperação do passivo ambiental e proteção dos recursos hídricos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)