Avaliação da concentração de flúor e do consumo de água mineral

OBJETIVO: Considerando a água como importante fonte de ingestão de flúor, o aumento da prevalência de fluorose dentária e do consumo de água mineral, o estudo realizado teve como objetivo avaliar o consumo de água mineral e sua concentração de flúor. MÉTODOS: A pesquisa foi realizada nos bairros do município de Bauru, SP. Foram visitadas mil residências que compunham uma amostra estratificada, obtida por conglomerados. Cada conglomerado correspondia a um quarteirão. Para efeito de sorteio os quarteirões foram numerados dentro das 17 regiões estabelecidas no plano diretor do município. Para coleta das amostras de água mineral foram usados frascos plásticos de 50 ml, previamente identificados. Na análise de flúor utilizou-se eletrodo íon sensível (Orion 9609) após tamponamento com TISAB II. Informações a respeito do consumo de água mineral foram obtidas com a aplicação de um questionário. RESULTADOS: Constatou-se que cerca de 29,7% da população do município consome água mineral. Nas 260 amostras analisadas, das 29 diferentes águas, a concentração de flúor variou de 0,045 a 1,515 mg/l. Em uma das águas, constava no rótulo o valor de 0,220 mg/l, mas na análise constatou-se 1,515 mg/l de flúor. Além disso, algumas águas não traziam especificado a concentração de flúor; e, na análise constataram-se valores variando entre 0,049 e 0,924 mg/l. CONCLUSÕES: Os resultados mostraram uma grande variação na concentração de flúor e reforçam a importância do controle dessas águas por parte da vigilância sanitária.

Geoquímica de radionuclídeos naturais e biomonitoramento em área de mineração de carvão no sul do Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Marine palaeo-ecological and depositional processes on the shallow-waters of the Azores archipelago: the mobile Ostracoda (Crustacea) and the sessile Bryozoa as case-studies

Tese de Doutoramento, Biologia (Taxonomia Zoológica), 11 de Outubro de 2013, Universidade dos Açores.

Estudo da desgaseificação difusa no Vulcão das Furnas (Ilha de S. Miguel) : o caso do ²²²Rn

Dissertação de Mestrado, Vulcanologia e Riscos Geológicos, 20 de Fevereiro de 2008, Universidade dos Açores.

Intra- and interspecific mineral composition variability of commercial instant coffees and coffee substitutes: contribution to mineral intake

The present paper reports the amount and estimated daily mineral intake of nine elements (Ca, Mg, K, Na, P, Fe, Mn, Cr and Ni) in commercial instant coffees and coffee substitutes (n = 49). Elements were quantified by high-resolution continuum source flame (HR-CS-FAAS) and graphite furnace (HR-CS-GFAAS) atomic absorption spectrometry, while phosphorous was evaluated by a standard vanadomolybdophosphoric acid colorimetric method. Instant coffees and coffee substitutes are rich in K, Mg and P (>100 mg/100 g dw), contain Na, Ca and Fe in moderate amounts (>1 mg/100 g), and trace levels of Cr and Ni. Among the samples analysed, plain instant coffees are richer in minerals (p < 0.001), except for Na and Cr. Blends of coffee substitutes (barley, malt, chicory and rye) with coffee (20–66%) present intermediate amounts, while lower quantities are found in substitutes without coffee, particularly in barley. From a nutritional point of view the results indicate that the mean ingestion of two instant beverages per day (total of 4 g instant powder), either with or without coffee, cannot be regarded as important sources of minerals to the human diet, although providing a supplementation of some minerals, particularly Mg and Mn in instant coffees. Additionally, and for authentication purposes, the correlations observed between some elements and the coffee percentage in the blends, with particular significance for Mg amounts, provides a potential tool for the estimation of coffee in substitute blends.

Fatores associados à baixa densidade mineral óssea em mulheres brancas

OBJETIVO: Analisar se os fatores para baixa densidade mineral óssea em mulheres idosas são os mesmos observados em outras faixas etárias. MÉTODOS: Realizou-se estudo transversal em amostra aleatória de prontuários de 413 mulheres brancas assistidas em serviço de diagnóstico por imagem, na cidade de Santos, estado de São Paulo, em 2003. Foram considerados os valores de densidade mineral óssea femoral ajustada pelo T-score. Foram investigadas as variáveis: idade, índice de massa corporal, tabagismo, consumo de álcool e leite, atividade física e terapia de reposição hormonal. Empregou-se regressão logística não condicional uni e multivariada. RESULTADOS: Na amostra, 52,5% tinham até 59 anos e 47,5% tinham 60 anos ou mais. O valor médio da densidade mineral óssea foi 0,867 g/cm² (dp=0,151) para o colo do fêmur. Valores significativos, ajustados pela idade foram obtidos para atividade física (OR ajustada=0,47; IC 95%: 0,23;0,97), índice de massa corporal igual ou superior a 30,0 kg/m² (OR ajustada=0,10; IC 95%: 0,05;0,21), etilismo (OR ajustada=7,90; IC 95%: 2,17;28,75), pouco consumo de leite (OR ajustada=3,29; IC 95%: 1,91;5,68) e reposição hormonal (OR ajustada=0,44; IC 95%: 0,21;0,90). Em mulheres idosas, massa corporal, consumo de leite e atividade física foram fatores de proteção independentes. CONCLUSÕES: Idade avançada, massa corporal, atividade física, consumo de leite e álcool foram importantes fatores na regulação da massa óssea. A influência de fatores comportamentais se manteve nas mulheres em idade avançada, reforçando o papel das medidas preventivas na prática médica e das políticas de promoção de saúde voltadas ao envelhecimento saudável.

Contribuição para o estudo da composição mineral de alguns vinhos açorianos comercializados

Dissertação de Mestrado, Engenharia Agronómica, 22 de Fevereiro de 2016, Universidade dos Açores.

Survey of trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) in retail samples of flavoured and bottled waters

Concentrations of eleven trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) were measured in 39 (natural and flavoured) water samples. Determinations were performed using graphite furnace electrothermetry for almost all elements (Al, As, Cd, Cr, Co, Mn, Ni, Pb, and Si). For Se determination hydride generation was used, and cold vapour generation for Hg. These techniques were coupled to atomic absorption spectrophotometry. The trace element content of still or sparkling natural waters changed from brand to brand. Significant differences between natural still and natural sparkling waters (p<0.001) were only apparent for Mn. The Mann–Whitney U-test was used to search for significant differences between flavoured and natural waters. The concentration of each element was compared with the presence of flavours, preservatives, acidifying agents, fruit juice and/or sweeteners, according to the labelled composition. It was shown that flavoured waters generally increase the trace element content. The addition of preservatives and acidifying regulators had a significant influence on Mn, Co, As and Si contents (p<0.05). Fruit juice can also be correlated to the increase of Co and As. Sweeteners did not provide any significant difference in Mn, Co, Se and Si content.

Electrochemical determination of antioxidant capacities in flavored waters by guanine and adenine biosensors

The immobilization and electro-oxidation of guanine and adenine asDNA bases on glassy carbon electrode are evaluated by square wave voltammetric analysis. The influence of electrochemical pretreatments, nature of supporting electrolyte, pH, accumulation time and composition of DNA nucleotides on the immobilization effect and the electrochemical mechanism are discussed. Trace levels of either guanine or adenine can be readily detected following short accumulation time with detection limits of 35 and 40 ngmL−1 for guanine and adenine, respectively. The biosensors of guanine and adenine were employed for the voltammetric detection of antioxidant capacity in flavored water samples. The method relies on monitoring the changes of the intrinsic anodic response of the surface-confined guanine and adenine species, resulting from its interaction with free radicals from Fenton-type reaction in absence and presence of antioxidant. Ascorbic acid was used as standard to evaluate antioxidant capacities of samples. Analytical data was compared with that of FRAP method.

Development of an FIA system with amperometric detection for determination of bentazone in estuarine waters

On the basis of its electrochemical behaviour a new flow-injection analysis (FIA) method with amperometric detection has been developed for quantification of the herbicide bentazone (BTZ) in estuarine waters. Standard solutions and samples (200 µL) were injected into a water carrier stream and both pH and ionic strength were automatically adjusted inside the manifold. Optimization of critical FIA conditions indicated that the best analytical results were obtained at an oxidation potential of 1.10 V, pH 4.5, and an overall flow-rate of 2.4 mL min–1. Analysis of real samples was performed by means of calibration curves over the concentration range 2.5x10–6 to 5.0x10–5 mol L–1, and results were compared with those obtained by use of an independent method (HPLC). The accuracy of the amperometric determinations was ascertained; errors relative to the comparison method were below 4% and sampling rates were approximately 100 samples h–1. The repeatability of the proposed method was calculated by assessing the relative standard deviation (%) of ten consecutive determinations of one sample; the value obtained was 2.1%.

Amperometric and spectrophotometric determination of carbaryl in natural waters and commercial formulations

The work presented describes the development and evaluation of two flow-injection analysis (FIA) systems for the automated determination of carbaryl in spiked natural waters and commercial formulations. Samples are injected directly into the system where they are subjected to alkaline hydrolysis thus forming 1-naphthol. This product is readily oxidised at a glassy carbon electrode. The electrochemical behaviour of 1-naphthol allows the development of an FIA system with an amperometric detector in which 1-naphthol determination, and thus measurement of carbaryl concentration, can be performed. Linear response over the range 1.0×10–7 to 1.0×10–5 mol L–1, with a sampling rate of 80 samples h–1, was recorded. The detection limit was 1.0×10–8 mol L–1. Another FIA manifold was constructed but this used a colorimetric detector. The methodology was based on the coupling of 1-naphthol with phenylhydrazine hydrochloride to produce a red complex which has maximum absorbance at 495 nm. The response was linear from 1.0×10–5 to 1.5×10–3 mol L–1 with a detection limit of 1.0×10–6 mol L–1. Sample-throughput was about 60 samples h–1. Validation of the results provided by the two FIA methodologies was performed by comparing them with results from a standard HPLC–UV technique. The relative deviation was <5%. Recovery trials were also carried out and the values obtained ranged from 97.0 to 102.0% for both methods. The repeatability (RSD, %) of 12 consecutive injections of one sample was 0.8% and 1.6% for the amperometric and colorimetric systems, respectively.

Electrochemical DNA-sensor for evaluation of total antioxidant capacity of flavours and flavoured waters using superoxide radical damage

In this paper, a biosensor based on a glassy carbon electrode (GCE) was used for the evaluation of the total antioxidant capacity (TAC) of flavours and flavoured waters. This biosensor was constructed by immobilising purine bases, guanine and adenine, on a GCE. Square wave voltammetry (SWV) was selected for the development of this methodology. Damage caused by the reactive oxygen species (ROS), superoxide radical (O2·−), generated by the xanthine/xanthine oxidase (XOD) system on the DNA-biosensor was evaluated. DNA-biosensor encountered with oxidative lesion when it was in contact with the O2·−. There was less oxidative damage when reactive antioxidants were added. The antioxidants used in this work were ascorbic acid, gallic acid, caffeic acid, coumaric acid and resveratrol. These antioxidants are capable of scavenging the superoxide radical and therefore protect the purine bases immobilized on the GCE surface. The results demonstrated that the DNA-based biosensor is suitable for the rapid assess of TAC in beverages.

Flavored waters: influence of ingredients on antioxidant capacity and terpenoid profile by HS-SPME/GC-MS

The antioxidant profiles of 39 water samples (29 flavored waters based on 10 natural waters) and 6 flavors used in their formulation (furnished by producers) were determined. Total phenol and flavonoid contents, reducing power, and DPPH radical scavenging activity were the optical techniques implemented and included in the referred profile. Flavor extracts were analyzed by HS-SPME/GC-MS to obtain the qualitative and quantitative profiles of the volatile fraction of essential oils. Results pointed out a higher reducing power (0.14 11.8 mg of gallic acid/L) and radical scavenging activity (0.29 211.5 mg Trolox/L) of flavored waters compared with the corresponding natural ones, an interesting fact concerning human health. Bioactive compounds, such as polyphenols, were present in all samples (0.5 359 mg of gallic acid/L), whereas flavonoids were not present either in flavored waters or in flavors. The major components of flavor extracts were monoterpenes, such as citral, R-limonene, carveol, and R-terpineol.

Pilot monitoring study of ibuprofen in surface waters of north of Portugal

Ibuprofen is amongst the most worldwide consumed pharmaceuticals. The present work presents the first data in the occurrence of ibuprofen in Portuguese surface waters, focusing in the north area of the country, which is one of the most densely populated areas of Portugal. Analysis of ibuprofen is based on pre-concentration of the analyte with solid phase extraction and subsequent determination with liquid chromatography coupled to fluorescence detection. A total of 42 water samples, including surface waters, landfill leachates,Wastewater Treatment Plant (WWTP), and hospital effluents, were analyzed in order to evaluate the occurrence of ibuprofen in the north of Portugal. In general, the highest concentrations were found in the river mouths and in the estuarine zone. The maximum concentrations found were 48,720 ngL−1 in the landfill leachate, 3,868 ngL−1 in hospital effluent, 616 ngL−1 in WWTP effluent, and 723 ngL−1 in surface waters (Lima river). Environmental risk assessment was evaluated and at the measured concentrations only landfill leachates reveal potential ecotoxicological risk for aquatic organisms. Owing to a high consumption rate of ibuprofen among Portuguese population, as prescribed and nonprescribed medicine, the importance of hospitals, WWTPs, and landfills as sources of entrance of pharmaceuticals in the environment was pointed out. Landfill leachates showed the highest contribution for ibuprofen mass loading into surface waters. On the basis of our findings, more studies are needed as an attempt to assess more vulnerable areas.

Origin and evolution of Upper Triassic to Miocene clay-mineral associations from the eastern Algarve of Portugal

XRD-analyses of pelitic deposits of Upper Jurassic to Miocene age occuring in the eastern Algarve (Portugal), give evidence of the occurrence of detrital clay minerals of continental origin as well as of conspicuous neoformations of marine provenance. The vertical succession of clay-mineral associations indicates the existence of three distinctive evolutionary cycles which are thought to reflect tectonically controlled transgressive-regressive events.