Neural stem cells adopt tumorigenic properties by constitutively activated NF-kappaB and subsequent VEGF up-regulation

One of the challenges in stem cell research is to avoid transformation during cultivation. We studied high passage subventricular zone derived neural stem cells (NSCs) cultures of adult rats in the absence of growth factors epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). We termed this culture exogenous growth factor independent neural stem cells (GiNSCs). GiNSCs expressed stemness markers, displayed a high constitutive NF-kappaB activity and an increased, aberrant, polyploid DNA content. GiNSCs showed a tumorigenic phenotype and formed colonies in a soft agar assay. Microarray analysis showed the up-regulation of the NF-kappaB target gene vascular endothelial growth factor (VEGF). In contrast, proneuronal genes were down-regulated. Under neuronal differentiation conditions GiNSCs adopted a glioma-like phenotype, with nuclear p53, preserving high amounts of Nestin positive cells and prolonged proliferation. Neutralization of VEGF strongly inhibited proliferation and induced differentiation. In a gain of function approach, the transfection of NSCs with constitutively active upstream kinase IKK-2 led to constitutively activated NF-kappaB, proliferation in absence of growth factors and augmented VEGF secretion. In a rescue experiment a reduction of NF-kappaB activity by overexpression of IkappaB-AA1 was able to shift the morphology toward an elongated cell form, increased cell death, and decreased proliferation. Thus GiNSCs may provide a potent tool in cancer research, as their exogenous cytokine independent proliferation and their constitutively high NF-kappaB expression presumes cancerous properties observed in gliomas. In addition, this study might add a novel mechanism for detecting oncogenic transformation in therapeutic stem cell cultures.

En retorisk analys av Fahrenheit 9/11 ur ett dokumentärhistoriskt perspektiv

The history of rhetorics in documentary film is a long one. The French brothers Louise and Auguste Lumiére were among the first persons who produced a documentary and their work has had a big impression in the history of documentary during the 1900 century. John Grierson has been called “the father” of documentary film. He produced films in the English speaking part of the world and he is the founder of the concept “documentary film”. Propaganda filming has a big part in the history of documentary film. Most frequently it appeard in Germany before and during the secound WW II, but propaganda films has been produced in Great Britain and USA as well as in other parts of the world. Rhetorics are of current interest today. It effects the news, papers, magazines, books and movies. The purpose with this essay is to investigate the rhetorics in Michael Moores controversial documentary of September 11th, Fahrenheit 9/11. The main question is: what rhetorical tools does Michael Moore use in order to communicate his politcal message in Fahrenheit 9/11? The rhetorical analysis includes exordium, narratio, propsitio, argumentatio and conclusio as well as ethos, logos and pathos. In order to conduct the analysis I have used Kurt Johannesson’s book Retorik eller konsten att övertyga, Maria Karlberg and Brigitte Mral’s book Heder och påverkan. Att analysera modern retorik. And at last a chapter by Brigitte Mral named Retorikanalys in the book Metoder i kommunikationeverenskap, by Mats Ekström and Larsåke Larsson. For the background about the history of documentary film I have used Barsam M. Richard, Nonfiction Film A Critical History and Bjørn Sørenssen’s book Å fange virkeligheten –Dokumentarfilmens århundre.The analysis shows that Moore uses different rhetorical tools in order to point out that George W Bush is the wrong man for the role as the president of United States of America. Some of the most effective tools that Moores uses are his own voice, pictures who awakes emotions, recordings and sharp arguments. His way of using ethos, logos and pathos is very clear. The disposal can also be distinct discern, however there are some exceptions towards the classical rhetoric. One example is that Moore doesn´t describe the arrangement of the movie in exordium.

Health problems in elderly patients during the first post-stroke year

BACKGROUND: A wide range of health problems has been reported in elderly post-stroke patients. AIM: The aim of this study was to analyse the prevalence and timing of health problems identified by patient interviews and scrutiny of primary health care and municipality elderly health care records during the first post-stroke year. METHODS: A total of 390 consecutive patients, ≥65 years, discharged alive from hospital after a stroke event, were followed for 1 year post-admission. Information on the health care situation during the first post-stroke year was obtained from primary health care and municipal elderly health care records and through interviews with the stroke survivors, at 1 week after discharge, and 3 and 12 months after hospital admission. RESULTS: More than 90% had some health problem at some time during the year, while based on patient record data only 4-8% had problems during a given week. The prevalence of interview-based health problems was generally higher than record-based prevalence, and the ranking order was moderately different. The most frequently interview-reported problems were associated with perception, activity, and tiredness, while the most common record-based findings indicated pain, bladder and bowel function, and breathing and circulation problems. There was co-occurrence between some problems, such as those relating to cognition, activity, and tiredness. CONCLUSIONS: Almost all patients had a health problem during the year, but few occurred in a given week. Cognitive and communication problems were more common in interview data than record data. Co-occurrence may be used to identify subtle health problems.

Life situations and the care burden for stroke pa­tients and their informal caregivers in a prospective cohort study

Background. The purpose of this study was to analyse whether the parallel life situation between stroke patients and their informal caregivers (dyads) shown in cross-sectional studies prevails also in a longitudinal perspective. Methods. A total of 377 Swedish stroke patients, aged ‡65 years, and their 268 informal caregivers were followed from hospital admission and one year on. Analyses were based on patient interviews, functional ability (MMSE) score, Nottingham Health Profile (NHP) score, Hospital Anxiety and Depression (HAD) score, self-rated health score, and the Gothenburg Quality of Life (GQL) activity score. Similar information was obtained by postal questionnaires from informal caregivers, also including information on the nature and amount of assistance provided and on Caregiver Burden (CB) score. Results. Before index admission informal caregivers provided care on average 5 h per week and after discharge 11 h per week (P < 0.0001). Support volume was associated with patient sex (more for men), low patient’s functional ability, low received municipal social service support, closeness of patient–caregiver relation, and short distance to patient’s home. Significant positive associations within the dyads were found for HAD anxiety score (P < 0.0001), total NHP score (P < 0.0001), and GQL activity score (P < 0.0001) after adjustment for patient’s age, sex, functional ability, and patient–caregiver relationship. CB score increased with amount of informal caregiver support, patient’s age, and with low functional ability and low amount of municipal social service support. All these associations were constant across time. Conclusions. There was an association within the dyads regarding anxiety score, NHP score, and activity score. CB score was generally high.

Från traditionellt akademiskt bibliotek till modern lärmiljö : Högskolan Dalarnas bibliotek – förväntningar och resultat

I denna rapport beskrivs idéerna bakom Högskolan Dalarnas prisbelönta bibliotek i Falun. Planerings- och byggprocessen beskrivs och det färdiga resultatet utvärderas, samt de erfarenheter som har gjorts delas.

From a traditional academic library to a modern learning environment : Dalarna University Library - expectations and results

This report describes the ideas and vision behind Dalarna University's award-winning library in Falun. A description of the planning and construction processes and an evaluation of the final outcome are presented together with experiences and observations drawn from the project.

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A construção de autoridade monetária e democracia: a experiência brasileira no contexto da integração econômica em escala global

Este relatório apresenta os primeiros resultados semestrais de pesquisa junto a um grupo de cientistas políticos focando a política monetária (A construção de autoridade monetária e democracia: A experiência brasileira no contexto da integração econômica em escala global, FAPESP Processo no 2001/05568-8). Durante este primeiro semestre o trabalho de pesquisa consistiu em uma revisão geral da bibliografia sobre a política monetária e instituições na ciência política e na economia, como também a criação de novos bancos de dados sobre credito, moeda, e instituições financeiras no Brasil de 1860 a 2002 e, finalmente, o desenvolvimento e aplicação de uma pesquisa de opinião à uma amostra de 75 Deputados Federais brasileiros.

A presidência brasileira em perspectiva comparada II

Este relatório apresenta resultados semestrais de pesquisa sobre a administração da presidência brasileira. Uma perspectiva comparada baseada em estudos clássicos da presidência americana foi usada como referência para indagar sobre a história e a organização da presidência brasileira. O avanço central durante as pesquisas deste semestre foi o desenvolvimento de uma perspectiva conceitual e teórica que enfoca a presidência no contexto da crescente separação dos poderes na democracia brasileira. O relatório também apresenta resultados preliminares do estudo da história organizacional da presidência brasileira, a organização de um banco de dados sobre as medidas provisórias e o processo decisório, e considerações iniciais sobre governança e políticas públicas durante o governo de Fernando Henrique Cardoso.

A presidência brasileira em perspectiva comparada

Esta pesquisa utilizou estudos clássicos sobre a presidência Americana para organizar um estudo da história, da organização, e da administração da presidência Brasileira. Uma resenha crítica da bibliografia sobre presidencialismo abre o relatório, seguida por uma discussão de pesquisas sobre as presidências Americana e Brasileira. O relatório termina com uma discussão preliminar da presidência durante o primeiro mandato do Presidente Fernando Henrique Cardoso.

A presidência brasileira e a separação dos poderes

Este relatório apresenta os resultados iniciais de uma pesquisa de longo prazo sobre a presidência Brasileira e os outros poderes da federação. Várias pesquisas realizadas separadamente enfatizam a crescente importância do executivo, legislativo, e judiciário na política Brasileira desde a Constituição de 1988. Este relatório argumenta que, tomadas como um todo, estas pesquisas mostram um processo decisório mais complexo mais contestado, e mais democrático. Uma resenha das bibliografias recentes sobre as relações entre os três poderes no Brasil e nos Estados Unidos mostra a necessidade de mais pesquisa comparada sobre a separação dos poderes nestes dois países. O relatório também comunica os passos iniciais na organização de um banco de dados sobre sobre tendências de gastos entre os poderes da união a partir de dados oficiais sobre o Orçamento federal de 1995-2002.

É ainda o século das caixas econômicas? um estudo sobre bancos federais, autoridade monetária e democracia

Esta pesquisa visa examinar questões gerais sobre autoridade monetária, bancos públicos, e democracia através de um estudo histórico e institucional da Caixa Econômica Federal. Uma análise preliminar da história, da organização, e das tendências recentes das Caixas Econômicas brasileiras introduz uma discussão das tendências recentes nos mercados de crédito brasileiros. A organização de bancos de dados nesta primeira etapa da pesquisa permitirá uma comparação da atuação de bancos privados, públicos, e estrangeiros durante o período recente de 1994-2001, como também uma discussão sobre as políticas de crédito e de poupança depois de que as instituições financeiras públicas federais foram saneadas em 22 de junho de 2001.

Enfoque japonês na administração da tecnologia: as empresas caminhando para novos desafios com o desenvolvimento tecnológico

Trata-se de conceitos e estratégias adotados pelas empresas japonesas com suporte governamental a superar constantes desafios com base numa bem sucedida Administração da Tecnologia. O Japão historicamente se deparou com inúmeros conflitos internos e externos que forjou um povo laborioso e voluntarioso. que conduz o país rumo á vanguarda tecnológica do século XXI

A educação profissional no Brasil entre o Estado e a sociedade civil: evolução, debate atual e experiência recente

Esta dissertação debruça-se sobre a educação profissional brasileira no tocante a sua evolução, debate atual e experiência recente. No que se refere à evolução, revi sita esta modalidade de educação, desde sua origem até os dias atuais. No que tange ao debate atual, elucida as principais idéias/argumentos que permeiam os debates atuais e instigam a disputa social em torno da educação profissional no país. E, finalmente, no que concerne a experiência recente, a partir da análise de uma política pública do Mec, mostra como se faz à expansão e modernização da educação profissional no Brasil.