A Devonian (Givetian) fore-reef slope sequence at Liangshuijing and implication for Devonian platform-to-depression development in Guilin, South China

Three Bahama-like carbonate plaforms-the Guilin, Yangshuo and Yanshan-occurred in Guilin and the surrounding regions during Middle and Late Devonian, which, at a broad scale, are part of an extensive carbonate platform (Xiangzhou carbonate platform) facies in South China. The intraplatform depression facies, a unique characteristic of the Chinese Devonian depositional sequence, separates Bahama-like (platform-to-depression) carbonate subplatfonns. Intraplatform depressions resulted from syndepositional faulting that cut the basement of carbonate subplatforms and affected further platform development. The Liangshuijing section, located between the Guilin platform in the north and the Yangshuo platform in the south, is representative of the fore-reef slope facies neighboring an intraplatform. depression. The South edge of the fore-reef slope lies adjacent to the Yangshuo reef carbonate platform, and the north edge graded into the Yangdi pelagic depression facies. A detailed sedimentary and microfacies analysis work done in this study at the Liangshuijing section shows a distinct vertical facies change from back-reef, restricted platform, hemipelagic, to fore-reefslope facies, differing from either shallow-water benthic facies or typical pelagic facies. Various benthic and pelagic lithofacies and their associations have been recognized in the Liangshuijing section, including dolomitic rudstone, gastropod wackestone, Amphipora floatstone, tentaculitoid wackestone, stromatolite and oncoid limestone, Amphipora grainstone, grain flows, laminated limestone, flat-pebble and brachiopod floatstone, and carbonate turbidites. Eight types of sedimentary cycles composed of two or three lithofacies have been distinguished, which are able to indicate environment changes. Stromatolites, oncoids, grain flows, carbonate turbidites, and tentaculitoid limestones characterize the slope and intraplatform depression lithofacies. Analysis of the vertical sedimentary cycles in the Liangshuijinag section and the lateral stratigraphic equivalents suggest the differing facies patterns occurred at the middle Varcus Zone (Givetian) of Middle Devonian, coeval with the development of fore-reef slope facies in the Guilin area in response to syndeposifional faulting.

Secondary structure of the third extracellular loop responsible for ligand selectivity of a mammalian gonadotropin-releasing hormone receptor

The extracellular loop 3 (ECL3) of the mammalian gonadotropin-releasing hormone receptor (GnRH-R) contains an acidic amino acid (Glu(301) in the mouse GnRH-R,) that confers agonist selectivity for Are in mammalian GnRH. It is proposed that a specific conformation of ECL3 is necessary to orientate the carboxyl side chain of the acidic residue for interaction with Arg(8) of GnRH, which is supported by decreased affinity for Arg(8) GnRH but not Gln(8) GnRH when an adjacent Pro is mutated to Ala. To probe the structural contribution of the loop domain to the proposed presentation of the carboxyl side chain, we synthesized a model peptide (CGPEMLNRVSEPGC) representing residues 293-302 of mouse ECL3, where Cys and Gly residues are added symmetrically at the N and C termini, respectively, allowing the introduction of a disulfide bridge to simulate the distances at which the ECL3 is tethered to the transmembrane domains 6 and 7 of the receptor. The ability of the ECL3 peptide to bind GnRH with low affinity was demonstrated by its inhibition of GnRH stimulation of inositol phosphate production in cells expressing the GnRH-R. The CD bands of the ECL3 peptides exhibited a superposition of predominantly unordered structure and partial contributions from beta-sheet structure. Likewise, the analysis of the amide I and amide III bands from micro-Raman and FT Raman experiments revealed mainly unordered conformations of the cyclic and of the linear peptide. NMR data demonstrated the presence of a beta-hairpin among an ensemble of largely disordered structures in the cyclic peptide. The location of the turn linking the two strands of the hairpin was assigned to the three central residues L-296, N-297, and R-298. A small population of structured species among an ensemble of predominantly random coil conformation suggests that the unliganded receptor represents a variety of structural conformers, some of which have the potential to make contacts with the ligand. We propose a mechanism of receptor activation whereby binding of the agonist to the inactive receptor state induces and stabilizes a particular structural state of the loop domain, leading to further conformational rearrangements across the transmembrane domain and signal propagating interaction with G proteins. Interaction of the Glu(301) of the receptor with Arg(8) of GnRH induces a folded configuration of the ligand. Our proposal thus suggests that conformational changes of both ligand and receptor result from this interaction.

Aerosolization of protein solutions using thermal inkjet technology

Vapotronics Inc. is developing the thermal inkjet (TIJ) technology used extensively in the printer industry to create a digital aerosol inhaler for the inhalation of therapeutics for local and systemic delivery. The operation of thermal inkjet printers requires generation of high temperatures and vaporization of the liquid formulation to effect droplet ejection. A study was conducted to develop formulations that would permit the generation of aerosols of therapeutic proteins without damage to the inkjet system or degradation of the proteins. Two proteins, human growth hormone and insulin, were formulated and aerosolized. The aerosol was collected and subjected to assays to compare the physicochemical and biological activities of these proteins before and after aerosolization. In each case, there was no significant changes to the proteins as a result of the aerosolization, providing evidence that TIJ can be used for aerosolizing solutions of protein therapeutics.

Development of a quality use of medicines coding system to rate clinical pharmacists' medication review recommendations

Objective: To develop a 'quality use of medicines' coding system for the assessment of pharmacists' medication reviews and to apply it to an appropriate cohort. Method: A 'quality use of medicines' coding system was developed based on findings in the literature. These codes were then applied to 216 (111 intervention, 105 control) veterans' medication profiles by an independent clinical pharmacist who was supported by a clinical pharmacologist with the aim to assess the appropriateness of pharmacy interventions. The profiles were provided for veterans participating in a randomised, controlled trial in private hospitals evaluating the effect of medication review and discharge counselling. The reliability of the coding was tested by two independent clinical pharmacists in a random sample of 23 veterans from the study population. Main outcome measure: Interrater reliability was assessed by applying Cohen's kappa score on aggregated codes. Results: The coding system based on the literature consisted of 19 codes. The results from the three clinical pharmacists suggested that the original coding system had two major problems: (a) a lack of discrimination for certain recommendations e. g. adverse drug reactions, toxicity and mortality may be seen as variations in degree of a single effect and (b) certain codes e. g. essential therapy were in low prevalence. The interrater reliability for an aggregation of all codes into positive, negative and clinically non-significant codes ranged from 0.49-0.58 (good to fair). The interrater reliability increased to 0.72-0.79 (excellent) when all negative codes were excluded. Analysis of the sample of 216 profiles showed that the most prevalent recommendations from the clinical pharmacists were a positive impact in reducing adverse responses (31.9%), an improvement in good clinical pharmacy practice (25.5%) and a positive impact in reducing drug toxicity (11.1%). Most medications were assigned the clinically non-significant code (96.6%). In fact, the interventions led to a statistically significant difference in pharmacist recommendations in the categories; adverse response, toxicity and good clinical pharmacy practice measured by the quality use of medicine coding system. Conclusion: It was possible to use the quality use of medicine coding system to rate the quality and potential health impact of pharmacists' medication reviews, and the system did pick up differences between intervention and control patients. The interrater reliability for the summarised coding system was fair, but a larger sample of medication regimens is needed to assess the non-summarised quality use of medicines coding system.

Reliability of moderate-intensity and vigorous physical activity stage of change measures for young adults

Background. The purpose of this study was to examine the reliability of stage of change (SOC) measures for moderate-intensity and vigorous physical activity in two separate samples of young adults. Staging measures have focused on vigorous exercise, but current public health guidelines emphasize moderate-intensity activity. Method. For college students in the USA (n = 105) and in Australia (n = 123), SOC was assessed separately on two occasions for moderate-intensity activity and for vigorous activity. Test-retest repeatability was determined, using Cohen's kappa coefficient. Results. In both samples, the reliability scores for the moderate-intensity physical activity staging measure were lower than the scores for the vigorous exercise staging measure. Weighted kappa values for the moderate-intensity staging measure were in the fair to good range for both studies (0.50 and 0.45); for the vigorous staging measure kappa values were excellent and fair to good (0.76 and 0.72). Conclusions. There is a need to standardize and improve methods for staging moderate-intensity activity, given that such measures are used in public health interventions targeting HEPA (health-enhancing physical activity). (C) 2003 American Health Foundation and Elsevier Science (USA). All rights reserved.

Los desaf??os de las pol??ticas y la gesti??n social en Am??rica Latina

La pobreza est?? aumentando en t??rminos absolutos de manera sistem??tica desde 1990 y, desde comienzos del nuevo siglo, tambi??n en valores relativos. Hay, sin embargo, una buena nueva: el gasto p??blico social ha aumentado tambi??n de manera sistem??tica desde entonces. ??Qu?? se ha hecho con la enorme magnitud de recursos destinada a financiar las pol??ticas sociales? Hay, por un lado, limitaciones que surgen de la forma en que ellas se dise??an e implementan. La gesti??n social tradicional asume que el impacto perseguido se producir?? autom??ticamente como resultado de la mera entrega de los bienes o servicios a la poblaci??n destinataria. Aparece, adem??s, la masiva introducci??n de mecanismos de mercado en las pol??ticas sociales, bajo el supuesto que permitir??an mejorar su eficiencia e impacto. Teniendo en cuenta las limitaciones precedentes, se sugieren en este art??culo tres senderos, estos son: la superaci??n del estilo tradicional de gestionar los programas y proyectos sociales; el aprendizaje por medio de la experiencia de la evaluaci??n de impacto y del monitoreo; y la generaci??n de un estilo m??s participativo en el proceso de dise??o, gesti??n y evaluaci??n de las pol??ticas sociales.

Estudo da anatomia do recesso frontal por meio de dissecção endoscópica em cadáveres

INTRODUÇÃO E OBJETIVO: O óstio do seio frontal freqüentemente apresenta difícil reconhecimento devido a estruturas anatômicas que encobrem sua visibilização. O objetivo principal desse estudo foi identificar e descrever as estruturas anatômicas do recesso frontal que dificultam o reconhecimento do óstio do seio frontal. CASUÍSTICA E MÉTODOS: Foi realizado um estudo prospectivo por meio de dissecção endoscópica consecutiva de 32 cadáveres (59 fossas nasais), 10 (31,25%) do sexo feminino e 22 (68,75%) do sexo masculino. Após exérese endoscópica da porção inferior do processo uncinado, com a preservação da sua inserção superior, avaliamos quais estruturas anatômicas necessitavam ser removidas até a completa visibilização endonasal do óstio do seio frontal. RESULTADOS E CONCLUSÃO: A visibilização do óstio do seio frontal após a exérese da porção inferior do processo uncinado foi possível em apenas 11 (18,64%) fossas nasais. O processo uncinado (recesso terminal) representou a principal estrutura anatômica que dificultou o reconhecimento endonasal do óstio do seio frontal, ocorrendo em 45 (76,27%) fossas nasais, seguido pela bolha etmoidal (16,95%) e a célula agger nasi (6,78%).

Evaluation of collaborative strategies for ecotourism and recreational activities in natural parks of Rio de Janeiro

In the city of Rio de Janeiro, the management agencies of environmental conservation units of the park type have been attempting to meet five primary objectives set by the National System for Conservation Units (NSCU), using participatory management guidelines for these units. Two of these objectives relate to the development of recreation activities that involve contact with nature and ecological tourism. This article presents the analyses and conclusions regarding the implementation of collaborative strategies with businesses to achieve such objectives; it is part of a series of research studies having a broader scope. Case studies were conducted in eight parks by means of dozens of interviews with managers and other interested social actors, as well as by documentary research and direct observation. The results suggest that the ecotourism objective is still far from being reached, and that the collaborative strategies used are not sufficient to compensate for the organizational, material and human limitations that encumber these agencies. It was also concluded for the sample that there lacks a strategic vision on the part of the three branches of government involved in the management of these parks in the sense of viewing ecotourism in the city's conservation units as a powerful means to foster local sustainable development.

Personalidade e percepção de stress em bombeiros

RESUMO: O objectivo deste estudo consistiu na obtenção de dados que ajudem a melhor compreender as diversas correlações entre traços de personalidade e a percepção de stress em bombeiros, de modo a que seja possível identificar factores desencadeantes de stress, bem como traços de personalidade que reforcem positiva ou negativamente esses mesmos factores . A amostra é constituída por 302 bombeiros pertencentes a corporações das regiões do Ribatejo e da Extremadura, sendo que todos os participantes desempenham funções de bombeiro voluntário ou profissional/efectivo. De entre os bombeiros participantes 238 são do sexo masculino e 64 são do sexo feminino tendo idades compreendidas entre os 17 e os 73 anos (m= 32,75 ; dp=9,309). As medidas utilizadas neste estudo são as seguintes: Marlow-Crowne Social Desirability Scale – Short Form C (MCSDS-C) de Ballard (1992), para a avaliação da desejabilidade social; Perceived Stress Scale (PSS) de Cohen, Kamarck & Mermelstein (1983), para a avaliação do stress e o Revised NEO Personality Inventory (NEO PI-R) de Costa & McCrae (1992) , para a avaliação da personalidade. Os resultados obtidos demonstraram associações significativas entre as dimensões da personalidade, nomeadamente o neuroticismo, a extroversão, a abertura à experiência e a conscienciosidade, e o stress. Verificaram-se correlações positivas entre as dimensões de neuroticismo e abertura à experiência relativamente ao stress, ao invés das dimensões de extroversão e conscienciosidade que por sua vez se correlacionaram negativamente com o stress. Este estudo demonstra também que os bombeiros profissionais(efectivos apresentam um mais elevado nível de stress comparativamente aos bombeiros pertencentes à categoria de voluntários, tal como os bombeiros com nove ou menos anos na função comparativamente aos bombeiros com mais de nove anos na função. Verificou-se também que os bombeiros do sexo masculino apresentam menos índices de stress comparativamente aos do sexo feminino. ABSTRACT: The purpose of this study was to obtain data can help us to better understand the various correlations between personality traits and the perception of stress in firefighters in order to identify trigger stress factors and personality traits which increase positively or negatively these same factors. The sample consists of 302 firefighters belonging to corporations within the regions of Ribatejo and Extremadura, being that all participants are either professional/effective or volunteer firefighters. Among the participating firefighters 238 are males and 64 are females, having aged between 17 and 73 years (m=32.75; dp=9.309). The measures used in this study are the following: Marlow-Crowne Social Desirability Scale – Short Form C (MCSDS-C) by Ballard (1992), for the assessment of social desirability; Perceived Stress Scale (PSS) by Cohen, Kamarck & Mermelstein (1983), for the assessment of stress and the Revised NEO Personality Inventory (NEO PI-R) by Costa & McCrae (1992) , for the assessment of personality. The results obtained showed significant associations between the personality dimensions of neuroticism, extraversion, openness and conscientiousness, and stress. The dimensions of neuroticism and openness are positively correlated to stress, on the other hand, the dimensions of extraversion and conscientiousness are negatively correlated to stress. Results also show that professional/effective firefighters have a higher level of stress as compared to volunteer firefighters as do firefighters with nine or fewer years in function compared with firefighters with more than nine years in function. It was also noted that male fire fighters present minor stress levels comparatively to female firefighters.