DEVELOPMENT OF INTEGRATED BIOPROCESSING TECHNOLOGIES FOR THE PRODUCTION OF PECTIC OLIGOSACCHARIDES (POS) FROM AGRO-PROCESSING RESIDUES

This research deals with the production of pectic oligosaccharides (POS) from agro-industrial residues, with specific focus on development of continuous cross flow enzyme membrane reactor. Pectic oligosaccharides have recently gained attention due to their prebiotic activity. Lack of information on the continuous production of POS from agro-industrial residues formed the basis for the present study. Four residues i.e sugar beet pulp, onion hulls, pressed pumpkin cake and berry pomace were taken to study their pectin content. Based on the presence of higher galacturonic acid and arabinose (both homogalacturonan and rhamnogalacturonan) in sugar beet pulp and galacturonic acid (only homogalacturonan) in onion hulls, further optimization of different extraction methods of pectin (causing minimum damage to pectic chain) from these residues were done. The most suitable extractant for sugar beet pulp and onion hulls were nitric acid and sodium hexametaphosphate respectively. Further the experiments on the continuous production of POS from sugar beet pulp in an enzyme membrane reactor was initiated. Several optimization experiments indicated the optimum enzyme (Viscozyme) as well as feed concentration (25 g/L) to be used for producing POS from sugar beet pulp in an enzyme membrane reactor. The results highlighted that steady state POS production with volumetric and specific productivity of 22g/L/h and 11 g/gE/h respectively could be achieved by continuous cross flow filtration of sugar beet pulp pectic extract over 10 kDa membrane at residence time of 20 min. The POS yield of about 80% could be achieved using above conditions. Also, in this thesis preliminary experiments on the production and characterization of POS from onion hulls were conducted. The results revelaed that the most suitable enzyme for POS production from onion hulls is endo-polygalacturonase M2. The POS produced from onion hulls were present in the form of DP1 -DP10 in substituted as well as unsubstituted forms. This study clearly demonstrates that continuous production of POS from pectin rich sources can be achieved by using cross flow continuous enzyme membrane reactor.

Geographical variation in carbon dioxide fluxes from soils in agro-ecosystems and its implications for life-cycle assessment

1. Exchange of carbon dioxide (CO2) from soils can contribute significantly to the global warming potential (GWP) of agro-ecosystems. Due to variations in soil type, climatic onditions and land management practices, exchange of CO2 can differ markedly in different geographical locations. The food industry is developing carbon footprints for their products necessitating integration of CO2 exchange from soils with other CO2 emissions along the food chain. It may be advantageous to grow certain crops in different geographical locations to minimize CO2 emissions from the soil, and this may provide potential to offset other emissions in the food chain, such as transport. 2. Values are derived for the C balance of soils growing horticultural crops in the UK, Spain and Uganda. Net ecosystem production (NEP) is firstly calculated from the difference in net primary production (NPP) and heterotrophic soil respiration (Rh). Both NPP and Rh were estimated from intensive direct field measurements. Secondly, net biome production (NBP) is calculated by subtracting the crop biomass from NEP to give an indication of C balance. The importance of soil exchange is discussed in the light of recent discussions on carbon footprints and within the context of food life-cycle assessment (LCA). 3. The amount of crop relative to the biomass and the Rh prevailing in the different countries were the dominant factors influencing the magnitude of NEP and NBP. The majority of the biomass for lettuce Lactuca sativa and vining peas Pisum sativum, was removed from the field as crop; therefore, NEP and NBP were mainly negative. This was amplified for lettuces grown in Uganda (-16·5 and -17 t C ha-1 year-1 compared to UK and Spain -4·8 to 7·4 and -5·1 to 6·3 t C ha-1 year-1 for NEP and NBP, respectively) where the climate elevated Rh. 4. Synthesis and applications. This study demonstrates the importance of soil emissions in the overall life cycle of vegetables. Variability in such emissions suggests that assigning a single value to food carbon footprints may not be adequate, even within a country. Locations with high heterotrophic soil respiration, such as Spain and Uganda (21·9 and 21·6 t C ha-1 year-1, respectively), could mitigate the negative effects of climate on the C costs of crop production by growth of crops with greater returns of residue to the soil. This would minimize net CO2 emissions from these agricultural ecosystems.

Az „iránymutató vízió” szerepe a regionális fejlesztésekben, egy hazai agráripari vállalkozás tapasztalatai (The role of „guiding vision“ on regional developments and experiences of a domestic agro-industrial enterprise)

A szerző tanulmányában külföldi és hazai példák alapján a fenntartható gazdaság kialakítására hoz példákat. Bemutat egy osztrák energiarégiót és egy magyar biodízelgyártó vállalkozást. Mindegyik esetre jellemző, hogy olyan társadalmi-gazdasági környezetet kell teremteni, hogy minden stakeholder a win-win megoldásban legyen érdekelt. _____ Agricultural land ownership and the desirable scale of operation have been the subjects of a plethora of studies. Mainstream research, however, has a tendency not to take the human factor into consideration. The unpredictability of economic policies, uncertainties about EU subsidies, the optimal scale of operation and industry- specific characteristics all constitute a far more exciting and reasonable research topic for the majority. According to literature, social support for the efforts and the existence of a clear “guiding vision” have a crucial role in the success of rural development strategies. Concerning the development of a region or village, it is important to determine whether there exists a leading personality, an example-setting entrepreneur or entrepreneurial group that can act as a fundamental driving force or an initiator in reforming the rural way of life; one that could help preserve positive rural values while nurturing economically successful enterprises. Experience has shown that success can only be built upon partnership and mutual cooperation.

An agro-ecological simulation model system

In this paper five different models, as five modules of a complex agro-ecosystem are investigated. The water and nutrient flow in soil is simulated by the nutrient-in-soil model while the biomass change according to the seasonal weather aspects, the nutrient content of soil and the biotic interactions amongst the other terms of the food web are simulated by the food web population dynamical model that is constructed for a piece of homogeneous field. The food web model is based on the nutrient-in-soil model and on the activity function evaluator model that expresses the effect of temperature. The numbers of individuals in all phenological phases of the different populations are given by the phenology model. The food web model is extended to an inhomogeneous piece of field by the spatial extension model. Finally, as an additional module, an application of the above models for multivariate state-planes, is given. The modules built into the system are closely connected to each other as they utilize each other’s outputs, nevertheless, they work separately, too. Some case studies are analysed and a summarized outlook is given.

Science behind management of Shark Bay and Florida Bay, two P-limited subtropical systems with different climatology and human pressures

This special issue on ‘Science for the management of subtropical embayments: examples from Shark Bay and Florida Bay’ is a valuable compilation of individual research outcomes from Florida Bay and Shark Bay from the past decade and addresses gaps in our scientific knowledge base in Shark Bay especially. Yet the compilation also demonstrates excellent research that is poorly integrated, and driven by interests and issues that do not necessarily lead to a more integrated stewardship of the marine natural values of either Shark Bay or Florida Bay. Here we describe the status of our current knowledge, introduce the valuable extension of the current knowledge through the papers in this issue and then suggest some future directions. For management, there is a need for a multidisciplinary international science program that focusses research on the ecological resilience of Shark Bay and Florida Bay, the effect of interactions between physical environmental drivers and biological control through behavioural and trophic interactions, and all under increased anthropogenic stressors. Shark Bay offers a ‘pristine template’ for this scale of study.

Nutritional status of lactating mothers and their children 6-23 months of age in pre- and post-harvest seasons in two agro-ecological zones of rural Ethiopia

The objective of this study was to assess seasonal variation in nutritional status and feeding practices among lactating mothers and their children 6-23 months of age in two different agro-ecological zones of rural Ethiopia (lowland zone and midland zone). Food availability and access are strongly affected by seasonality in Ethiopia. However, there are few published data on the effects of seasonal food fluctuations on nutritional status and dietary diversity patterns of mothers and children in rural Ethiopia. A longitudinal study was conducted among 216 mothers in two agro-ecological zones of rural Ethiopia during pre and post-harvest seasons. Data were collected on many parameters including anthropometry, blood levels of haemoglobin and ferritin and zinc, urinary iodine levels, questionnaire data regarding demographic and household parameters and health issues, and infant and young child feeding practices, 24 h food recall to determine dietary diversity scores, and household use of iodized salt. Chi-square and multivariable regression models were used to identify independent predictors of nutritional status. A wide variety of results were generated including the following highlights. It was found that 95.4% of children were breastfed, of whom 59.7% were initially breastfed within one hour of birth, 22.2% received pre-lacteal feeds, and 50.9% of children received complementary feedings by 6 months of age. Iron deficiency was found in 44.4% of children and 19.8% of mothers. Low Zinc status was found in 72.2% of children and 67.3% of mothers. Of the study subjects, 52.5% of the children and 19.1% of the mothers were anaemic, and 29.6% of children and 10.5% of mothers had iron deficiency anaemia. Among the mothers with low serum iron status, 81.2% and 56.2% of their children had low serum zinc and iron, respectively. Similarly, among the low serum zinc status mothers, 75.2% and 45.3% of their children had low serum in zinc and iron, respectively. There was a strong correlation between the micronutrient status of the mothers and the children for ferritin, zinc and haemoglobin (P <0.001). There was also statistically significant difference between agro-ecological zones for micronutrient deficiencies among the mothers (p<0.001) but not for their children. The majority (97.6%) of mothers in the lowland zone were deficient in at least one micronutrient biomarker (zinc or ferritin or haemoglobin). Deficiencies in one, two, or all three biomarkers of micronutrient status were observed in 48.1%, 16.7% and 9.9% of mothers and 35.8%, 29.0%, and 23.5%, of children, respectively. Additionally, about 42.6% of mothers had low levels of urinary iodine and 35.2% of lactating mothers had goitre. Total goitre prevalence rates and urinary iodine levels of lactating mothers were not significantly different across agro-ecological zones. Adequately iodised salt was available in 36.6% of households. The prevalence of anaemia increased from post-harvest (21.8%) to pre-harvest seasons (40.9%) among lactating mothers. Increases were from 8.6% to 34.4% in midland and from 34.2% to 46.3% in lowland agro-ecological zones. Fifteen percent of mothers were anaemic during both seasons. Predictors of anaemia were high parity of mother and low dietary diversity. The proportion of stunted and underweight children increased from 39.8% and 27% in post-harvest season to 46.0% and 31.8% in pre-harvest season, respectively. However, wasting in children decreased from 11.6% to 8.5%. Major variations in stunting and underweight were noted in midland compared to lowland agroecological zones. Anthropometric measurements in mothers indicated high levels of undernutrition. The prevalence of undernutrition in mothers (BMI <18.5kg/m2) increased from 41.7 to 54.7% between post- and pre-harvest seasons. The seasonal effect was generally higher in the midland community for all forms of malnutrition. Parity, number of children under five years and regional variation were predictors of low BMI among lactating mothers. There were differences in minimum meal frequency, minimum acceptable diet and dietary diversity in children in pre-harvest and post-harvest seasons and these parameters were poor in both seasons. Dietary diversity among mothers was higher in lowland zone but was poor in both zones across the seasons. In conclusion, malnutrition and micronutrient deficiencies are very prevalent among lactating mothers and their children 6-23 months old in the study areas. There are significant seasonal variations in malnutrition and dietary diversity, in addition to significant differences between lowland and midland agro-ecological zones. These findings suggest a need to design effective preventive public health nutrition programs to address both the mothers’ and children’s needs particularly in the preharvest season.

Melt climatology estimates for small to giant Antarctic icebergs, links to NetCDF files

We present four melt climatology estimates based on a simulation of Antarctic iceberg drift and melting that includes small, medium-sized, and giant tabular icebergs with a realistic size distribution. Drift and meltdown is simulated using vertical profiles of ocean currents, temperature, and salinity, which goes beyond the present standard in iceberg modeling. The climatology estimates based on simulations of small (SMA), 'small-to-medium'-sized (MED12 & MED123), and small-to-giant icebergs (ALL) exhibit differential characteristics: successive inclusion of larger icebergs leads to a reduced seasonality of iceberg melt and a shift of the mass input to the area north of 58°S, while less melt water is released into the coastal areas. This highlights the necessity to account for larger and giant icebergs in order to obtain accurate melt climatologies. The four monthly melt climatologies [mm/day] are available as netCDF files with 1°x1° spatial resolution and can be used, e.g., for sensitivity studies with uncoupled sea ice-ocean models, or as spatio-temporal templates for the redistribution of land ice from the Antarctic ice sheet over the Southern Ocean in climate models.

Resíduos da indústria agro-alimentar como possíveis fontes de ácido ferúlico

A indústria agro-alimentar produz anualmente uma grande quantidade de subprodutos, ainda tratados como desperdício. A indústria cervejeira, em particular, está associada à produção de vários resíduos, entre os quais o bagaço de malte, também designado bagaço de cerveja ou dreche. Sendo produzida numa razão de 20 kg por cada 100 litros de cerveja, só na Ilha da Madeira a produção de dreche ultrapassa as 2000 toneladas/ano. O presente trabalho foi realizado com o intuito de estudar a utilização da dreche como material de partida para a extracção de ácido ferúlico, um ácido hidroxicinâmico com elevada bioactividade e aplicações. A caracterização físico-química da dreche permitiu determinar um teor de humidade de aproximadamente 70% e um teor de cinzas de cerca de 3,6%. A distribuição granulométrica da dreche seca, revelou que cerca de 70% das partículas que a constituem têm dimensão entre 1 e 0,25 mm. A extracção com acetona produziu um extracto contendo 5 compostos de natureza fenólica, determinados por LC-MS. A hidrólise alcalina – uma das técnicas que permitem a extracção de compostos como o ácido ferúlico a partir de matrizes lenhocelulósicas – foi estudada em amostras de dreche submetidas a tratamento prévio. O pré-tratamento com ácido diluído demonstrou ser eficiente na extracção do ácido ferúlico a partir da dreche. A extracção em autoclave mostrou ser eficiente na extracção do ácido ferúlico [0,28% (m/m)] e uma simplificação do procedimento posterior à reacção de hidrólise alcalina fez aumentar o rendimento de extracção em cerca de 84%, comparativamente ao procedimento habitual. As condições óptimas de hidrólise alcalina em tubos autopressurizados aconteceram a 120 ºC, por 1,5 horas, num rácio de 20 mL/g e NaOH (1,5%). O processo de purificação do ácido ferúlico extraído por adsorção numa resina sintética resultou em percentagens de adsorção de 90,83% e de dessorção em torno dos 68,70%.

Riesgos y prevención para trabajos en espacios confinados en ámbitos del agro y la agroindustria

El objetivo de este trabajo es generar un documento que brinde elementos que contribuyan al conocimiento de los factores de riesgo ligados a ambiente laborales en espacios confinados en el agro y sus agroindustrias. Esto se persigue como objetivo, en resguardo de los trabajadores frente a aquellos factores de riesgo que puedan generar accidentes, muchas veces mortales o enfermedades profesionales vinculadas a la contaminación por polvo, ruido, escasez de oxígeno o por la exposición a contaminantes químicos que podrían ser tóxicos o sensibilizantes de las vías respiratorias o la piel. Estos últimos, en muchas ocasiones, son la consecuencia de la presencia en dichos ambientes confinados de distintos productos agroquímicos, fundamentalmente insecticidas. Sin afectar el fin lógico de las empresas que buscan mejorar la productividad y la rentabilidad permanentemente, este trabajo se propone brindar algunos elementos de apoyo que permitan dar respuesta a las demandas de los mercados que exigen producir con adecuada calidad atendiendo además al bienestar de los trabajadores: garantizar la salud, seguridad y confort en el lugar en el que el operario busca el sustento, respetando asimismo el cuidado del ambiente y de las personas que habitan en él, que son condiciones ineludibles de cumplimiento obligado en toda sociedad responsable

Agro industrialización y comercialización de frijol rojo y negro en Unión de Cooperativas Agropecuarias Augusto César Sandino, San Ramón - Matagalpa: Informe de pasantías 2014

Como forma de culminación de estudios de la carrera de Licenciatura en Desarrollo Rural, se realizó pasantías en la Unión de Cooperativas Augusto César Sandino (UCA, San Ramón). En el proyecto tiene como nombre: Apoyo al desarrollo productivo y agroindustrial del Frijol en organizaciones cooperativas de pequeños productores y productoras de Matagalpa en el período de Abril a Octubre del 2014. La UCA San Ramón es una cooperativa comprometida con el desarrollo económico-social de sus asociados, apoyándoles en la formación de capacidades, promueve la equidad de género, protección del medio ambiente flora y fauna ,aplicación de las buenas prácticas agrícolas (BPM), Promoción del turismo rural sostenible, apoyo al sector agrícola en los diferentes rubros(Café, Granos Básicos, Ganado).La UCA San Ramón, también otorga becas a estudiantes hijos de socios para que culminen sus estudios y que retornen a trabajar en la institución con el propósito de mejorar el relevo generacional. El trabajo del pasante estuvo enfocado en el área de planificación y proyectos que se desarrollan dentro de la institución misma, los cuales están dirigidos a promover la formulación, gestión, evaluación y gerencia de proyectos sociales, para los socios, llevando a cabo la siguiente funcione: Elaboración de plan de Negocios, para el cual es el pasante desarrollo las siguientes funciones: Análisis de información primaria y secundaria; Sondeos para analizar la competencia en presentaciones de frijol (Crudos, molidos, cocidos)en supermercados, Capacitaciones en uso de maquinaria de planta procesadora de frijol; Registros y control de actividades de la planta. El plan de negocios que se diseñó dio como resultado la demostración de una factibilidad sobre la comercialización de frijol procesado en diferentes presentaciones conforme a las demandas de los consumidores, el análisis de información primaria permitió conocer la demanda sobre gustos y preferencias de los consumidores, según los sondeos realizados demuestran que el frijol crudo tiene mayor demanda, los registros y controles en la planta complementan los documentos realizados dentro de la institución misma, la realización de esta pasantía fue de gran importancia y lección aprendida ya que el pasante debe estar accesible a adquirir conocimientos novedosos que complementan su trabajo como profesional, el espíritu de responsabilidad y confianza es muy valioso al momento de llegar formalizar un acuerdo permite evitar conflictos en un futuro. Como conclusión se puede decir que a medida que se va ejecutando las pasantías hace familiar la labor y se va obteniendo nuevos conocimientos amplios sobre la misma; tratándose de un periodo de nuevas enseñanzas y experiencias que se basan en los conocimientos adquiridos en el aula de clase y para alcanzar mejores oportunidades en el campo laboral. Como una recomendación enfocada a la Universidad, es dar mejor seguimiento para los pasantes durante el periodo de pasantías constando en supervisión a este.

Produção enzimática de biodiesel a partir de resíduos agro-industriais usando a lipase imobilizada de origem microbiana

Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia Química e Biológica

Licenciamento Ambiental: uma ferramenta de marketing - O caso de uma empresa agro-alimentar

Mestrado em Gestão e Empreendedorismo

Papel das medidas agro-ambientais na gestão da paisagem rural no concelho de Marvão no PNSSM

As paisagens rurais portuguesas construídas e mantidas ao longo dos tempos por sistemas agrícolas tradicionais, estão hoje ameaçadas por motivos tão diversos como o envelhecimento da população, o abandono rural, a intensificação, a homogeneização dos sistemas de produção e a perda de competitividade. Mas apesar destes problemas, estas paisagens agrícolas, suportam ainda várias funções não produtivas, nomeadamente, constituem, um importante suporte de biodiversidade, pelo que a sua manutenção é importante para a conservação destes habitats e espécies. Eventualmente novas formas de gestão destas paisagens devem ser criadas, nomeadamente com base na combinação de várias funções numa perspectiva de multifuncionalidade, através de uma adaptação e integração de políticas públicas. Estando actualmente em discussão o novo programa de Desenvolvimento Rural e a definição das futuras Medidas Agro-Ambientais, e a gestão e o financiamento da Rede Natura 2000, estamos portanto num momento crítico para decisões futuras, que terão forçosamente que interligar, a agricultura, o ambiente e desenvolvimento das zonas rurais portuguesas. Com o intuito de melhor compreender estas problemáticas, em particular, as transformações em curso na paisagem rural, o papel das Medidas Agro-Ambientais e apresentar possíveis soluções, foi efectuado este estudo de caso no concelho de Marvão, concelho típico das áreas marginais agrícolas do interior sul de Portugal. ABSTRACT; The Portuguese rural landscapes built up and kept throughout the times by traditional agricultural systems, are today threatened by so diverse reasons as the ageing of the population, the agricultural abandonment, the intensification, the homogenization of the production systems and the loss of competitiveness. But despite these problems. These agricultural landscapes still support a multitude of non-commodity functions, and particularly they still constitute an important support of biodiversity and thus their maintenance is important for the conservation of these habitats and species. Probably new management forms must be created based on the combination of various functions and the adaptation and integration of public policies. Being currently in discussion the new program of Rural Development and the definition of the future Agri-Environmental Measures, and the management and the financing of the Natura 2000 Network, we are therefore at a critical moment for future decisions that will forcibly have to establish connections, between the agriculture, the environment and the development of the portuguese agricultural areas. With the intention of better understanding these problems and questions, , the transformations taking place in Portuguese peripheric rural areas, and in particular role of the Agri-Environmental Measures, and also for presenting possible solutions, a case study was analyzed in municipality of Marvão, characteristic of the agricultural marginal areas of the interior Southern Portugal.

Resíduos da indústria agro-alimentar como possíveis fontes de ácido ferúlico

A indústria agro-alimentar produz anualmente uma grande quantidade de subprodutos, ainda tratados como desperdício. A indústria cervejeira, em particular, está associada à produção de vários resíduos, entre os quais o bagaço de malte, também designado bagaço de cerveja ou dreche. Sendo produzida numa razão de 20 kg por cada 100 litros de cerveja, só na Ilha da Madeira a produção de dreche ultrapassa as 2000 toneladas/ano. O presente trabalho foi realizado com o intuito de estudar a utilização da dreche como material de partida para a extracção de ácido ferúlico, um ácido hidroxicinâmico com elevada bioactividade e aplicações. A caracterização físico-química da dreche permitiu determinar um teor de humidade de aproximadamente 70% e um teor de cinzas de cerca de 3,6%. A distribuição granulométrica da dreche seca, revelou que cerca de 70% das partículas que a constituem têm dimensão entre 1 e 0,25 mm. A extracção com acetona produziu um extracto contendo 5 compostos de natureza fenólica, determinados por LC-MS. A hidrólise alcalina – uma das técnicas que permitem a extracção de compostos como o ácido ferúlico a partir de matrizes lenhocelulósicas – foi estudada em amostras de dreche submetidas a tratamento prévio. O pré-tratamento com ácido diluído demonstrou ser eficiente na extracção do ácido ferúlico a partir da dreche. A extracção em autoclave mostrou ser eficiente na extracção do ácido ferúlico [0,28% (m/m)] e uma simplificação do procedimento posterior à reacção de hidrólise alcalina fez aumentar o rendimento de extracção em cerca de 84%, comparativamente ao procedimento habitual. As condições óptimas de hidrólise alcalina em tubos autopressurizados aconteceram a 120 ºC, por 1,5 horas, num rácio de 20 mL/g e NaOH (1,5%). O processo de purificação do ácido ferúlico extraído por adsorção numa resina sintética resultou em percentagens de adsorção de 90,83% e de dessorção em torno dos 68,70%.