Progresso científico sobre nutrição de animais de companhia na primeira década do século XXI

O conhecimento científico sobre nutrição de animais de companhia tem aumentado de forma contínua, acompanhando o fenômeno visto em diversas áreas de conhecimento. Na última década, têm-se prioritariamente pesquisas direcionadas ao uso de nutrientes na promoção de saúde, prevenção de doenças degenerativas, melhoria da qualidade de vida e aumento da expectativa de vida de cães e gatos. Este direcionamento de pesquisas é, em grande parte, explicado pela importância que cães e gatos assumiram na vida das pessoas, fazendo com que as decisões alimentares dos proprietários com seus animais se assemelhassem às que adotam para si próprios. A publicação da nova revisão do Nutrient Requirements of Dogs and Cats em 2006 foi, talvez, o avanço recente mais significativo, trazendo novo entendimento sobre necessidades energéticas e de nutrientes nas diferentes fases de desenvolvimento e estados fisiológicos. Apesar destes avanços, ainda são necessárias pesquisas na área de caracterização físico-química e de utilização dos ingredientes, efeitos do processo de extrusão e mesmo de necessidades nutricionais, que conta com uma base pequena de artigos disponíveis, a maioria antiga. Talvez os desafios científicos mais importantes em nutrição de cães e gatos sejam metabolismo de carboidratos, importância da massa corporal magra na saúde, urolitíases, gerontologia, relação entre microbiota intestinal e saúde, imunonutrição e manejo nutricional em condições clínicas específicas importantes para estas espécies.

Um estudo sobre a concepção de paradoxo segundo o pensamento de Augustus de Morgan

This work aims to analyze the concept of "paradox" posed in the work of The Budget Paradox (1872) of mathematical and logical English Augustus De Morgan (1806-1871). Here it is important to note that a large part of this book consists of re-prints of a series of writings by the author in journal Athenaeum, where its performance as auditor of literature. The tests refer to some scientific work produced between the years 1489 and 1866 and the rules of selection for the composition of the work is, basically, the methodological aspects used in the completion or disclosed by such scholars. The concept of paradox is presented in two distinct moments. At first, we found a study of definitions for the term in a philosophical approach, characterizing it as something that requires further investigation; which was complemented with the classic examples of a scientific context. In the second, we present a concept advocated by De Morgan and, under this perspective, that he conceptualized the "paradox" is directly related to the non-usual methods employed in the formulation of new scientific theories. In this study some of these scientific concepts are detailed, where, through the redemption history, engaging in issues of our study Mathematics, Physics, of Logic, among others. Possession of the preliminary analysis and comparison with the design of De Morgan, it became possible to diagnose some limitations in the conceptualization suggested by the author. Further, evidenced, in front of the cases, the nonlinearity of the process of production of knowledge and hence the progress of science

Conhecimentos e habilidades de acadêmicos sobre procedimentos em semiologia e semiotécnica da enfermagem

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Progresso científico em pequenos ruminantes na primeira década do século XXI

O interesse pelos pequenos ruminantes aumentou nos últimos anos sob os aspectos produtivo e científico. No Brasil, caprinos e ovinos são os que despertam maior interesse econômico. Assim, este trabalho foi conduzido com o objetivo de discutir o progresso científico com caprinos e ovinos nos últimos dez anos. Foram pesquisadas várias bases de dados, entre elas as do Institute for Scientific Information (ISI), da Scientific Electronic Library Online - SciELO, da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES, do Instituto Brasileiro de Geografia e Estatística - IBGE e da Food and Agriculture Organization of the United Nations - FAO. Na última década, os pequenos ruminantes têm merecido especial atenção dos pesquisadores, o que pode ser comprovado pelo aumento de 41% nas publicações mundiais envolvendo estas espécies, o que foi acompanhado por aumento ainda mais expressivo, 219%, em âmbito nacional. A evolução também foi observada sob os aspectos metodológicos dos projetos, ressaltando a abordagem multidisciplinar nas pesquisas mais atuais. Nos últimos dez anos, houve crescimento do aporte de recursos para editais temáticos demandados pela sociedade e pela cadeia da carne e do leite, além da preocupação com qualidade, competitividade e sustentabilidade.

Correlação entre condições de saneamento básico e parasitoses intestinais na população de Assis, Estado de São Paulo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeitos do ultrassom terapêutico sobre parâmetros morfométricos e clínicos e método de extração de metaloproteinases em sujeitos com úlceras venosas

Venous wounds cause physical, psychological and financial problems that impact the quality of life of patients. Treatment alternatives are investigated in order to reduce healthcare costs and improve quality of life of people affected by this problem. Physical resources, such as therapeutic ultrasound (US), are being considered in the treatment of ulcers as a potential healing agent. This study aimed to investigate the application of US as a treatment for venous ulcers. Subjects were divided into two groups: US group, where treatment consisted of 5 sessions of pulsed US (3 MHz, 1W/cm²) associated with compression and kinesiotherapy; and sham group, where individuals went through the same procedures, but with sham US therapy. Subjects were evaluated for wound size by planimetry and digital photography, visual analogue scale for pain, quality of life by the questionnaires SF- 36 and VEINES-QoL/Sym and enzymatic activity of metalloproteinases 2 and 9 by zymography. It was observed mean reduction in wound area of 41.58±53.8% for the US group and 63.47±37.2% for the placebo group, maintenance of quality of life scores in the US group and significant improvement (p<0.05) in the placebo group by VEINES questionnaire. It was observed decreased perception of pain in the placebo group. Sample feasibility for analysis of the protein activity of metalloproteinases 2 and 9 by zymography collected by swab method was also confirmed. Our data did not give us evidence to support the theory that the US accelerates healing of venous ulcers in a short-term analysis. However, we observed that standard care associated with compression therapy and kinesiotherapy were able to significantly shorten the progression of chronic venous ulcers

Expressão imuno-histoquímica metaloproteinase -1, -2 e -9 em ameloblastoma sólido e tumor odontogênico adematóide

Ameloblastoma and adenomatoid odontogenic tumor are odontogenic tumors arising from the odontogenic epithelium with distinct clinical behavior. In attempt to comprehend the interaction between the odontogenic tumor cells and the extracellular matrix, the present work evaluated and compared the immunohistochemical expression of the matrix metalloproteinases-1 (MMP-1), -2 (MMP-2) and -9 (MMP-9) in 20 cases of ameloblastoma and 10 adenomatoid odontogenic tumor. MMP-1 exhibited exuberant expression in the parenchyma and in the stroma of both studied tumors, while the MMP-2 showed varied expression with about of 80% and 60% of the neoplastic cells exhibiting positivity in the ameloblastoma and adenomatoid odontogenic tumor, respectively. With relation to the MMP-2 expression by the mesenchymal cells, it was observed that 65% of the ameloblastoma and 80% of the adenomatoid odontogenic tumor were positive. The immunoreactivity of MMP-9 was detected in all studied cases, although its expression had occurred predominantely in less than 50% of the parenchyma cells of the ameloblastoma, while in about of 60% of the adenomatoid odontogenic tumor more than 50% of cells were positive. The mesenchymal cells were positive to MMP-9 in 65% of the ameloblastoma and in 80% of the adenomatoid odontogenic tumor, respectively. Statistically significant difference was observed to the MMP-1 expression with relation to MMP-2 and MMP-9 in the ameloblastoma (p < 0.001). It was not possible to perform statistical analysis to the cases of adenomatoid odontogenic tumor, however there was a tendency toward a differential expression of the MMP-1 with relation to other studied MMPs. These results suggest that MMP-1, - 2 and -9 are implicated in the growth and progression of both tumors analyzed as well as the more pronounced participation of the stroma in the ameloblastoma could together to be related to the higher clinical aggressiveness

Imunoexpressão de galectinas em queilites actínias e sua relação com características clínicas e gradação histológica

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Estudo da influência de linfócitos tcd-8 e das células nk em casos de carcinoma epidermóide de lábio inferior sem metástase e com metástase e sua relação com a progressão da lesão

The presence of inflammatory cells within the tumor microenvironment plays a dual role that may contribute both to the progression and for inhibition of tumor growth. Recent studies suggest that the quality, not the quantity, of the inflammatory infiltrate is the most important determinant for prognosis. Therefore, TCD8 cells and natural killer cells are the main effector cells in combating cancer. The aim of this study was to assess, through the immunohistochemical study, the expression of TCD8 lymphocytes and NK cells in epidermoid carcinoma (EC) of the lower lip. The sample consisted of 32 specimens of EC of the lower lip, of which 16 had regional lymph node metastasis, and the 16 remaining, free of metastases. The total number of positive cells at the front of invasion were evaluated quantitatively and the results were related to clinical TNM staging, histological grade of malignancy and prognostic factors. It was observed for the group with metastasis, prevalence of stages III and IV (p<0.0001). Most patients with metastasis, had a high grade of malignancy (p=0.006). Most cases classified as high grade of malignancy had stages III and IV (p=0.032). Of the total sample, there were three cases of recurrence and five with death, however these variables were not statistically significant when associated with clinicopathological parameters. The immunostaining of CD8 and CD57, respectively, showed no statistically significant association with any of the clinicopathological parameters studied, metastasis (p=0.346, p=0.622), TNM classification (p=0.146, p=0.576), histological grade of malignancy (p=0.936, p=936), recurrence (p=0.075, p=0.075) and death (p=0.897, p=0.856). Believing in the function of the immunological system against malignant cells, it is concluded that the TD8 lymphocytes and NK cells, would be acting in the control of the progression of malignant neoplasms, but not in isolated manner

Estudo da expressão imuno-histoquímica das proteínas MMP-9, MMP-13 e TIMP-1 em ameloblastomas e tumores odontogênicos ceratocistos

Ameloblastomas and keratocystic odontogenic tumors (KOT) represent odontogenic lesions that, despite their benign nature, are distinguished by a distinct biological behavior, characterized by locally aggressive growth and recurrent episodes. The gnathic bone resorption caused by the growth of these lesions is a key to the expansion of the same, both being mediated by osteoclastic cells like enzymatic activity of various matrix metalloproteinases (MMPs) factor. The expression of stimulatory factors and inhibitors of bone resorption has been correlated with the development of these lesions, with emphasis to some MMPs such as collagenases and gelatinases and tissue inhibitors of metalloproteinases (TIMPs), among others. Based on the premise that stimulatory and inhibitory factors of osteolytic processes can be decisive for the growth rate of intraosseous odontogenic lesions, this experiment evaluated the immunoreactivity of MMP-9, -13 and TIMP-1 protein in the epithelium and mesenchyme of ameloblastoma and the KOT specimens, by a quantitative analysis of the immunoreactivity cells. Statistical analysis was performed using the Mann-Whitney and Wilcoxon tests with a significance level set at 5 %. Immunohistochemical expression of MMP-9, -13 and TIMP-1 was observed in 100% of cases both in the epithelium and in mesenchyme. The immunoreactivity in the epithelium of KOT and ameloblastomas revealed a predominance of score 3 for MMP-9 (p=0.382) and MMP-13 (p=0.069) and no statistically significance for TIMP-1, the latter being significantly higher immunoreactivity in ameloblastomas. In the mesenchyme, there was a higher score immunoreactivity of MMP-13 (p=0.031) in ameloblastomas in relation to KOT, whereas for MMP-9 and TIMP-1 no statistically significant difference (p=0.403 was observed, p=1.000). The calculation of the ratio of scores revealed expression of proteins in general, similarity of the lesions, a significant predominance of equal expression of TIMP-1 and MMP-9 was observed only in the epithelium of ameloblastoma. The marked immunostaining of MMP-9 , MMP-13 and TIMP-1 in epithelium and mesenchyme of the lesion indicate that these proteins involved in ECM remodeling required for tumor progression, however, specific differences in the expression of some of these proteins, are not sufficient to suggest differences in the biological behavior of ameloblastomas and KOTs

Estudo in vitro dos efeitos da BMP-2 e do seu antagonista Noggin sobre a proliferação e migração celulares em carcinoma epidermóide de língua

Oral squamous cell carcinoma (OSCC) is the most prevalent malignancy in the oral cavity and reach a large number of individuals, has become an important public health problem. Studies have demonstrated changes in pathway components BMP in various types of cancers as prostate, colon, breast, gastric and OSCCs. Is the current knowledge that these proteins may exert pro-tumor effect in more advanced stages of neoplastic development coming to favor progression and invasion tumor. The inhibition of the signaling pathway BMP-2 through its antagonists, have shown positive results of antitumor activity and use of Noggin may be a novel therapeutic target for cancer. Given this evidence and the few studies with BMP-2, Noggin and OSCC, the objective of this research was to evaluate the effect of BMP-2 and its antagonist Noggin on proliferation and migration cell in line of cell cultures of human tongue squamous cell carcinoma (SCC25). The study was divided in three groups, a control group, where SCC25 cells suffered no treatment, a BMP-2 group, in which cells were treated with 100ng/ml of BMP-2 and a group of cells that were treated with 100ng/ml of Noggin. For the proliferation assay and cell cycle were established three time intervals (24, 48 and 72 hours). Proliferative activity was investigated by trypan blue and cell cycle analysis by staining with propidium iodide flow cytometry. The potential for migration / invasion of SCC25 cells was performing by a cell invasion assay using Matrigel in a 48-hour interval. The proliferation curve showed a higher proliferation in cells treated with BMP-2 in 72 hours (p < 0.05), and lower overgrowth and cell viability in Noggin group. Recombinant proteins favored a greater percentage of cells in cell cycle phase Go/G1 with a statistically significant difference in the interval of 24 hours (p < 0.05). BMP- 2 produced a greater invasion of cells studied as well as its antagonist Noggin inhibits invasion of cells (p < 0.05). Thus, these results indicate that BMP-2 promotes malignant phenotype, dues stimulates proliferation and invasion of SCC25 cells and, its antagonist Noggin may be an alternative treatment, due to inhibit the tumor progression

Aspectos da produtividade do feijão correlacionados com atributos físicos do solo sob elevado nível tecnológico de manejo

No ano agrícola de 2006/07, no município de Selvíria (MS), foi analisada a produtividade do feijão em função de alguns atributos físicos de um Latossolo Vermelho distroférrico, cultivado nas condições de elevado nível tecnológico de manejo pelo sistema plantio direto irrigado com pivô central. O objetivo foi selecionar, entre os atributos pesquisados do solo, aquele com a melhor representatividade para explicar a variabilidade da produtividade agrícola. Foi instalada a malha geoestatística, para a coleta de dados do solo e da planta, com 117 pontos amostrais, numa área de 2.025 m² e declive homogêneo de 0,055 m m-1. do ponto de vista linear e espacial, a produtividade de feijão foi explicada em função da densidade de partículas e da umidade gravimétrica do solo, respectivamente. Assim, os valores de densidade de partículas entre 1,95 e 2,20 kg dm-3 e de umidade gravimétrica entre 0,205 e 0,239 kg kg-1; ambas coletadas na camada intermediária, resultaram em sítios com as mais elevadas produtividades (2.200-2.600 kg ha-1). Entretanto, para as condições do elevado nível tecnológico de manejo empregado, a produtividade média do feijão não atingiu seu máximo potencial, uma vez que o agravado estado de compactação no qual o solo se encontrava proporcionou à produtividade redução de 26,6 %. Dessa forma, os valores médios da densidade do solo e da resistência do solo à penetração, respectivamente nas faixas de 1,36-1,41 kg dm-3 e de 1,56-3,24 MPa, típicos de solos argilosos compactados, atestaram a substancial queda da produtividade de grãos de feijão.

Efeitos da laserterapia na atividade biológica de células de carcinoma epidermóide de língua humano

The low level laser therapy (LLLT) has shown to be effective in promoting the proliferation of different cells in vitro, including keratinocytes, osteoblasts, endothelial cells and stem cells. It has been speculated that the biostimulatory effect of LLLT could cause undesirable enhancement of tumor growth in neoplastic diseases, since the malignant cells are more susceptible to proliferative stimuli. Within this context, this study evaluated the effect of LLLT on epidermoid carcinoma of the tongue cell line (SCC25) proliferation and invasion. Cultured cells were irradiated with an InGaAIP diode laser, 660nm, 30mW using two energy densities (0.5J/cm2 and 1.0J/cm2). Proliferative activity was assessed through trypan blue staining method and through cell cycle analysis using flow cytometry. The invasive potential was measured through cell invasion assay using matrigel. Cyclin D1, E-cadherin, -catenin and MMP-9 expressions were analyzed by immunofluorescence and flow cytometry and related to the investigated biological activities. Proliferation curve demonstrated that SCC25 irradiated with 1.0J/cm2 had the highest proliferative rate when compared to the control group and the group irradiated with 0.5J/cm2 (p<0.05). LLLT affected cell cycle distribution and energy density of 1.0 J/cm2 promoted a higher percentage of cells in S/G2/M phases, with statistically significant differences at 24h interval (p<0.05). LLLT, mainly with 1.0J/cm2, revealed significantly higher potential for invasion and influenced the expression of cyclin D1, E-cadherin, -catenin and MMP-9, promoting the malignant phenotype. In conclusion, our results indicate that LLLT has an important stimulatory effect on proliferation and invasion of SCC25 cells, likely due to altered expression of proteins associated with these processes

Estudo integrado geológico/tecnológico de rochas ornamentais: os granitos flores e jacarandá, RN

The main purpose of the present study is to integrate a geological and technological investigation of ornamental rocks of the Flores and Jacarandá granites, which are located near the Afonso Bezerra city, in the north central part of the Rio Grande do Norte State. The study area encompasses four litho-stratigrafic units: a Gneiss-Migmatitic Complex(cristalline basement), which is mainly composed of banded gneisses, usually deformed as mylonitic rocks and with several migmatic features, an Augen Gneiss, which occurs as an elongated body that constitutes the Jacarandá granite, a small granite stock, which presents a semi-circular form, named Flores granite, composed of pink, fine to medium coarse rocks, and fine to coarse alluvial sediments, which form extensive areas of large fluvial deposits. The technological characterization of the Flores and Jacarandá granites, carried out through several tests, has as the main purpose the determination of petrographic, physical, and mechanic parameters that allowed the characterization of these rocks. The test followed procedures recommended by Brazilian (ABNT Associação Brasileira de Normas Técnicas) and foreigner institutions (ASTM American Society for Testing and Materials). The petrografhic analysis indicated that the rocks investigated are granite sensu estrictu, summing an average 85-90% modal. The Flores granite is the more felsic rock, which presents mafic content ∼ 10% and monzonitic composition. The Jacarandá granite is an Augen Gneiss rock that presents sienogranitic composition and mafic modal content ∼ 15%. Several technological tests carried out (alterability, physical indices, velocity of ultrasonic wave propagation, uniaxial compression, flection resistence, Amsler desgaste, and resistence to freezing and heating) indicated that parameters and values were identical for both granites investigated. These parameters and values are consistent with the Brazilian and international standards for siliciclastic rocks of ornamental use, as well as other Brazilian ornamental granites. The analysis of all results indicates that both the Flores and Jacarandá granites present good quality, and that they are indicated for ornamental use of revetment interior and exterior of buildings

Como acompanhar a progressão da competência comunicativa no aluno de Enfermagem

Este estudo teve como objetivo propor bases metodológicas para o acompanhamento da progressão da competência comunicativa no aluno de Enfermagem. Utilizando metodologia qualitativa e referencial teórico de comunicação interpessoal, desenvolvemos a pesquisa entrevistando 13 professores com experiência acumulada em atividades de ensino e pesquisa de comunicação em Enfermagem. Nos dados obtidos, os entrevistados relataram que o acompanhamento da progressão da competência comunicativa do aluno ocorre: observando a evolução das habilidades comunicativas nos alunos e pacientes; viabilizando momentos de expressão de pensamentos, sentimentos e percepções; fornecendo feedback sobre o desenvolvimento das habilidades comunicativas; estimulando-os ao desenvolvimento das habilidades em comunicação; realizando avaliação formativa e tutorando o aluno.