Identification and functional characterization of a novel monotreme - specific antibacterial protein expressed during lactation

Monotremes are the only oviparous mammals and exhibit a fascinating combination of reptilian and mammalian characters. They represent a component of synapsidal reproduction by laying shelled eggs which are incubated outside the mother’s body. This is accompanied by a prototherian lactation process, marking them as representatives of early mammals. The only extant monotremes are the platypus, and the short- and long- beaked echidnas, and their distributions are limited to Australia and New Guinea. Apart for a short weaning period, milk is the sole source of nutrition and protection for the hatchlings which are altricial and immunologically naive. The duration of lactation in these mammals is prolonged relative to the gestational length and period of incubation of eggs. Much of the development of monotreme young occurs in the non-sterile ex-utero environment. Therefore the role of milk in the growth, development and disease protection of the young is of significant interest. By sequencing the cDNA of cells harvested from monotreme milk, we have identified a novel monotreme- specific transcript, and the corresponding gene was designated as the EchAMP. The expression profile of this gene in various tissues revealed that it is highly expressed in milk cells. The peptides corresponding to the EchAMP protein have been identified in a sample of echidna milk In silico analysis indicated putative antimicrobial potential for the cognate protein of EchAMP. This was further confirmed by in vitro assays using a host of bacteria. Interestingly, EchAMP did not display any activity against a commensal gut floral species. These results support the hypothesis of enhancement of survival of the young by antimicrobial bioactives of mammary gland origin and thus emphasize the protective, non- nutritional role of milk in mammals.

An Iranian migrant mother's production of selves through her family photograph collection

In researching migrant identities, visual methodology offers much promise and yet there is a marked lack of recent research that makes use of visual methods. Previous studies of migrant identities have privileged verbal representations of identities. Gillian Rose's (2010) work with mothers and their family photographs in the United Kingdom, in which she describes the role of family photographs in both producing social subject positions and in maintaining family togetherness across distances, is a useful model for research into the construction and negotiation of migrant identities.

The literature on family photography suggests that it is usually the woman/mother who takes on the role of making the family photograph album; of narrating the family's story (Rose, 2010; Holland, 2009; 1991; Chambers, 2003). The family photograph collection, together with the participant's interwoven verbal interpretation, is a particularly relevant data source for use in identity research as there is the potential for key themes of place, mobilities and space to be explored at new depth and from a feminist perspective.

This paper will report on an ethics approved study of one Iranian migrant mother and her family photograph collection, focusing on her representation of the identities and subjectivities of herself and her children through photographs taken following migration to Australia. The paper will consider the participant's family photographs as both visual objects and visual-verbal narratives produced within historically and culturally situated discourses. It will explore how photographs and oral interpretations cohere to enable migrant mothers to re/produce selves. The paper will examine the production of subject positions specifically in relation to place, mobilities and space.

The research is situated within critical visual ethnography and is informed by a reflexive feminist approach. The meaning making of the photographs under study will be explored at the sites of production, image, and audience. The combined visual-verbal methods used in this study aim to provide a new contribution to the literature on migrant identities and form the basis of a scaled up research design of a larger cohort of mothers.

Catholic girls: the mother-daughter nexus

This paper examines Catholic girlhood, womanhood and the mother–daughter
relationship, and its socio-historical construction within a range of disparate
discourses. The aim of the paper is to deconstruct dominant patriarchal
constructions and images of femininity, particularly those embedded within the
doctrine of Catholicism. Moreover, the paper intends to reveal traces of maternal
connections and relations which are often hidden by more dominant discourses.
Rather than providing a historical account of Catholic girlhood, the object is to tell
a perspectived story of the local and contextual experiences of growing up and
being educated to be a ‘good Catholic woman’ in suburban Melbourne, Australia
in the 1920s and 1960s. In telling the story it is hoped that other women can
momentarily engage with this narrative of Catholic girlhood and the mother–
daughter relationship.

Exploring subject positions and multiple temporalities through an Iranian migrant mother's family photograph albums

 In this article, I draw on a research study of one Iranian migrant mother's generation of selves through her material configuration of her personal photograph albums and through our verbal reading of her photographs. The research engages in a visual-material feminist ethnographic approach, and is informed by the work of Gillian Rose and the understanding that family photographs are a means by which women negotiate subject positions. In this article, I discuss an unexpected finding of my research, the significance of multiple temporalities in a migrant mother's production of selves. The photo album practices of the participant mother of this study, ‘Parvin’, depart from the common social convention of mothers arranging their photograph albums to chart family growth following the model of milestones occurring over linear developmental time. Parvin does not limit herself to linear developmental time, but rather she mixes up photographs in her post-migration family albums to generate multiple temporalities woven together by an enveloping ‘mixed’ time. Drawing on both Julia Kristeva and Homi Bhaba's theories of temporality and the subject, I suggest that Parvin produces subject positions for self and family through a continual interweaving of a multitude of pasts into the present and through a subsuming of milestones within cyclical family time. Further, I suggest that through her generation of multiple temporalities, Parvin produces the subject position of ‘accommodating mother’. Finally, I highlight the potential afforded by considering the temporal and the spatial together in studies of migrant identity.

Changing images of mother/mothering

This paper examines how representations of the mother and mothering practices have altered between a generation of mothers and daughter. It also discusses the varied configurations of mother/mothering which occur at different times in women's lives, in other racial and ethnic situations, and which have been opened up by medical science through reproductive technology. Taking a broad definition of mothering, the paper points to the hierarchical division that have been created between women who pay, and are being paid for the care and education of their children in their early years. It argues the difficult and complex task for early childhood education and care is to keep pace and grapple with the ever-changing cirsumstances of those who nurture and care for the young.

Child weight status: Relationships with mother-child mealtime interactions and child eating behaviours

This doctoral thesis highlighted how mother-child interactions surrounding responsiveness, affect, maternal control and child compliance may be influential to children’s self-regulatory behaviours around food and eating, in turn impacting their weight. Support was found for the potential role of such interactions as risk factors in childhood overweight and obesity.

Metabolic remodelling in obesity and type 2 diabetes: pathological or protective mechanisms in response to nutrient excess?

Altered metabolism in tissues such as the liver, skeletal muscle and adipose tissue is observed in metabolic diseases characterized by nutrient excess and energy imbalance, such as obesity and type 2 diabetes. These alterations in metabolism can include resistance to the hormone insulin, lipid accumulation, mitochondrial dysfunction and transcriptional remodelling of major metabolic pathways. The underlying assumption has been that these same alterations in metabolism are fundamental to the pathogenesis of metabolic diseases. An alternative view is that these alterations in metabolism occur to protect cell and tissue viability in the face of constant positive energy balance. This speculative review presents evidence that many of the metabolic adaptations that occur in metabolic diseases characterized by nutrient excess can be viewed as protective in nature, rather than pathogenic per se for disease progression. Finally, we also briefly discuss the usefulness and potential pitfalls of therapeutic approaches that attempt to correct these same metabolic defects when energy balance is not altered, and the potential links between metabolic survival responses and other chronic diseases such as cancer.

Evolution of adolescent mental health in a rapidly changing socioeconomic environment: a review of mental health studies in adolescents in India over last 10 years

Background: Significant changes in the economic, familial and social support aspects in India have occurred in recent times, making it an interesting naturalistic setting to observe the effects of a dynamic socioeconomic environment on behavioral and emotional disorders in adolescents. Objective: This systematic review attempts to synthesize and evaluate the available evidence on mental health disorders and interventions in adolescents in India in last 10 years as well as identify conceptual trends and methodological lacunae in these studies. Method: A systematic search of electronic databases was performed in March 2014 and 27 school and community based studies evaluating behavioral problems, psychiatric morbidity, stress, suicide-related behaviors, depression, anxiety, aggression, self concept in adolescents in India were reviewed. Conclusion: There is a wide variation in the reported prevalence of psychiatric morbidity and behavioral problems in Indian adolescents. Some of the risk and protective factors are similar to those identified by other International studies in this age group. These include female gender, academic difficulties, parental fights, strained familial relationships, school absenteeism, school dropout and other school related factors. However, there are certain variables that appear to be context specific and need further investigation. These are mother's working status, studying in Government institutions or belonging to a nuclear family as risk factors and praying as a coping skill, parental involvement as a protective factor for psychiatric disorders. The suspected upward trend in the psychiatric morbidity in this age group needs more studies to be established. © 2014 Elsevier B.V.