Diversity and biotechnological potential of culturable bacteria from Brazilian mangrove sediment.

Mangrove ecosystems are environments subject to substantial degradation by anthropogenic activities. Its location, in coastal area, interfacing the continents and the oceans makes it substantially important in the prospection for biotechnological applications. In this study, we assessed the diversity of culturable bacteria present over the seasons at two depths (0-10 and 30-40 cm) in a mangrove sediment and in a transect area from the land to the sea. In total, 238 bacteria were isolated, characterized by Amplified Ribosomal DNA Restriction Analysis (ARDRA) and further identified, by Fatty Acid Methyl Esther (FAME-MIDI), into the orders of Vibrionales, Actinomycetales and Bacillales. Also the ability of the isolates in producing economically important enzymes (amylases, proteases, esterases and lipases) was evaluated and the order Vibrionales was the main enzymatic source.

Genetic diversity and connectivity remain high in Holothuria polii (Delle Chiaje 1823) across a coastal lagoon-open sea environmental gradient

Coastal lagoons represent habitats with widely heterogeneous environmental conditions, particularly as regards salinity and temperature,which fluctuate in both space and time. These characteristics suggest that physical and ecological factors could contribute to the genetic divergence among populations occurring in coastal lagoon and opencoast environments. This study investigates the genetic structure of Holothuria polii at a micro-geographic scale across theMar Menor coastal lagoon and nearbymarine areas, estimating the mitochondrial DNA variation in two gene fragments, cytochrome oxidase I (COI) and 16S rRNA (16S). Dataset of mitochondrial sequences was also used to test the influence of environmental differences between coastal lagoon andmarine waters on population genetic structure. All sampled locations exhibited high levels of haplotype diversity and low values of nucleotide diversity. Both genes showed contrasting signals of genetic differentiation (non-significant differences using COI and slight differences using 16S, which could due to different mutation rates or to differential number of exclusive haplotypes. We detected an excess of recent mutations and exclusive haplotypes, which can be generated as a result of population growth. However, selective processes can be also acting on the gene markers used; highly significant generalized additive models have been obtained considering genetic data from16S gene and independent variables such as temperature and salinity.

Investigating stratification, language diversity and mathematics classroom interaction

Research on the socio-political dimensions of language diversity in mathematics classrooms is under-theorised and largely focuses on language choice. These dimensions are, however, likely to influence mathematics classroom interaction in many other ways than participants’ choice of language. To investigate these influences, I propose that the notions ofheteroglossia, orders of indexicality and scale-jumping, can provide new theoretical tools with which to understand the links between classroom interaction and broader social patterns of marginalisation. To illustrate the utility of these ideas, I include some analysis of an episode observed in a sheltered elementary school second language mathematics classroom in Canada.

Minority type matters : ethnic diversity and tolerance in 29 European democracies

Past research has shown that having a large population of ethnic minorities beyond the neighborhood level arouses intolerance in the majority. However, this paper presents the argument that the effect of minority size on tolerance depends on minority type: the less subject the minority is to negative stereotyping, the more favorable the effect that minority size has on tolerance. In this study, a hierarchical linear model was applied to a dataset on advanced and emerging democracies in Europe. The analysis shows that when the duration and level of democracy are controlled for, ethnic tolerance was associated positively with native minority size and negatively with foreign population size.

Age diversity and firm performance in an emerging economy: Implications for cross-cultural human resource management

This study tests the effect of age diversity on firm performance among international firms. Based on the resource-based view of the firm, it argues that age diversity among employees will influence firm performance. Moreover, it argues that two contextual variables—a firm's level of market diversification and its country of origin—influence the relationship between age diversity and firm performance. By testing relevant hypotheses in a major emerging economy, that is, the People's Republic of China, this study finds a significant and positive effect of age diversity and a significant interactive effect between age diversity and firm strategy on profitability. We also find a significant relationship between age diversity and firm profitability for firms from Western societies, but not for firms from East Asian societies. The paper concludes by discussing the implications of this study's findings. © 2011 Wiley Periodicals, Inc.

Effect of cattle on Salmonella carriage, diversity and antimicrobial resistance in free-ranging wild boar (Sus scrofa) in northeastern Spain

Salmonella is distributed worldwide and is a pathogen of economic and public health importance. As a multi-host pathogen with a long environmental persistence, it is a suitable model for the study of wildlife-livestock interactions. In this work, we aim to explore the spill-over of Salmonella between free-ranging wild boar and livestock in a protected natural area in NE Spain and the presence of antimicrobial resistance. Salmonella prevalence, serotypes and diversity were compared between wild boars, sympatric cattle and wild boars from cattle-free areas. The effect of age, sex, cattle presence and cattle herd size on Salmonella probability of infection in wild boars was explored by means of Generalized Linear Models and a model selection based on the Akaike's Information Criterion. Prevalence was higher in wild boars co-habiting with cattle (35.67%, CI 95% 28.19-43.70) than in wild boar from cattle-free areas (17.54%, CI 95% 8.74-29.91). Probability of a wild boar being a Salmonella carrier increased with cattle herd size but decreased with the host age. Serotypes Meleagridis, Anatum and Othmarschen were isolated concurrently from cattle and sympatric wild boars. Apart from serotypes shared with cattle, wild boars appear to have their own serotypes, which are also found in wild boars from cattle-free areas (Enteritidis, Mikawasima, 4:b:- and 35:r:z35). Serotype richness (diversity) was higher in wild boars co-habiting with cattle, but evenness was not altered by the introduction of serotypes from cattle. The finding of a S. Mbandaka strain resistant to sulfamethoxazole, streptomycin and chloramphenicol and a S. Enteritidis strain resistant to ciprofloxacin and nalidixic acid in wild boars is cause for public health concern.

Regional diversity and economic performance

This thesis consists of an introductory chapter and four individual papers. In each paper the relationship between some form of spatial diversity and economic performance is analyzed. Diversity is treated as a potential source of externality effects, mainly in the form of knowledge spillovers. The first paper studies the impact of a broad range of spatial externalities on the productivity of manufacturing plants. While finding positive effects of specialization and competition, there is no support for positive spillovers of either related or unrelated industry diversity. The second paper argues that relatedness should be framed at the level of individuals and consequently should be measured in terms of, for example, education and occupation rather than industry belonging. The results show that educational- and occupational related diversity matter for regional productivity growth, while related industry diversity is positively related to employment growth. The third paper analyzes the importance of neighborhood related diversity, in terms of both industries and education, and internal human capital for firms’ propensity to innovate. The findings support that education and skills are strongly related to firm innovation. Additionally, firms in metropolitan regions are more innovative in neighborhoods with more related diversity in industries, while firms in rural regions seem to benefit more from related diversity in education. In the fourth paper, the location factor of interest is segregation, which may be regarded as inverse diversity. The results show that neighborhood segregation has a negative effect on individual employment. However, it is not the spatial separation of individuals with different backgrounds that causes lower employment but rather the distress of segregated neighborhoods.

Diversity and Stability in Food Webs : Impacts of Non-Equilibrium Dynamics, Topology and Variation

With progressive climate change, the preservation of biodiversity is becoming increasingly important. Only if the gene pool is large enough and requirements of species are diverse, there will be species that can adapt to the changing circumstances. To maintain biodiversity, we must understand the consequences of the various strategies. Mathematical models of population dynamics could provide prognoses. However, a model that would reproduce and explain the mechanisms behind the diversity of species that we observe experimentally and in nature is still needed. A combination of theoretical models with detailed experiments is needed to test biological processes in models and compare predictions with outcomes in reality. In this thesis, several food webs are modeled and analyzed. Among others, models are formulated of laboratory experiments performed in the Zoological Institute of the University of Cologne. Numerical data of the simulations is in good agreement with the real experimental results. Via numerical simulations it can be demonstrated that few assumptions are necessary to reproduce in a model the sustained oscillations of the population size that experiments show. However, analysis indicates that species "thrown together by chance" are not very likely to survive together over long periods. Even larger food nets do not show significantly different outcomes and prove how extraordinary and complicated natural diversity is. In order to produce such a coexistence of randomly selected species—as the experiment does—models require additional information about biological processes or restrictions on the assumptions. Another explanation for the observed coexistence is a slow extinction that takes longer than the observation time. Simulated species survive a comparable period of time before they die out eventually. Interestingly, it can be stated that the same models allow the survival of several species in equilibrium and thus do not follow the so-called competitive exclusion principle. This state of equilibrium is more fragile, however, to changes in nutrient supply than the oscillating coexistence. Overall, the studies show, that having a diverse system means that population numbers are probably oscillating, and on the other hand oscillating population numbers stabilize a food web both against demographic noise as well as against changes of the habitat. Model predictions can certainly not be converted at their face value into policies for real ecosystems. But the stabilizing character of fluctuations should be considered in the regulations of animal populations.

Development of molecular approaches to assess the diversity and abundance of marine nematodes assemblages for assessment of Good Environmental Status of the estuarine and marine ecosystems

In Europe, the concerns with the status of marine ecosystems have increased, and the Marine Directive has as main goal the achievement of Good Environmental Status (GES) of EU marine waters by 2020. Molecular tools are seen as promising and emerging approaches to improve ecosystem monitoring, and have led ecology into a new era, representing perhaps the most source of innovation in marine monitoring techniques. Benthic nematodes are considered ideal organisms to be used as biological indicator of natural and anthropogenic disturbances in aquatic ecosystems underpinning monitoring programmes on the ecological quality of marine ecosystems, very useful to assess the GES of the marine environment. dT-RFLP (directed Terminal-Restriction Fragment Length Polymorphism) allows to assess the diversity of nematode communities, but also allows studying the functioning of the ecosystem, and combined with relative real-time PCR (qPCR), provides a high-throughput semi-quantitative characterization of nematode communities. These characteristics make the two molecular tools good descriptors for the good environmental status assessment. The main aim of this study is to develop and optimize the dT-RFLP and qPCR in Mira estuary (SW coast, Portugal). A molecular phylogenetic analysis of marine and estuarine nematodes is being performed combining morphological and molecular analysis to evaluate the diversity of free-living marine nematodes in Mira estuary. After morphological identification, barcoding of 18S rDNA and COI genes are being determined for each nematode species morphologically identified. So far we generated 40 new sequences belonging to 32 different genus and 17 families, and the study has shown a good degree of concordance between traditional morphology-based identification and DNA sequences. These results will improve the assessment of marine nematode diversity and contribute to a more robust nematode taxonomy. The DNA sequences are being used to develop the dT-RFLP with the ability to easily process large sample numbers (hundreds and thousands), rather than typical of classical taxonomic or low throughput molecular analyses. A preliminary study showed that the digest enzymes used in dT-RFLP for terrestrial assemblages separated poorly the marine nematodes at taxonomic level for functional group analysis. A new digest combination was designed using the software tool DRAT (Directed Terminal Restriction Analysis Tool) to distinguished marine nematode taxa. Several solutions were provided by DRAT and tested empirically to select the solution that cuts most efficiently. A combination of three enzymes and a single digest showed to be the best solution to separate the different clusters. Parallel to this, another tool is being developed to estimate the population size (qPCR). An improvement in qPCR estimation of gene copy number using an artificial reference is being performed for marine nematodes communities to quantify the abundance. Once developed, it is proposed to validate both methodologies by determining the spatial and temporal variability of benthic nematodes assemblages across different environments. The application of these high-throughput molecular approaches for benthic nematodes will improve sample throughput and their implementation more efficient and faster as indicator of ecological status of marine ecosystems.

Genetic diversity and population structure of the sea lamprey (Petromyzon marinus L.) across its distributional range

Lampreys are a group of ancient vertebrates with 360 million years of existence. Throughout their evolution, they have acquired local adaptations to the colonized habitats, showing high plasticity and adaptive capacities. The sea lamprey (Petromyzon marinus L.) is a parasitic and anadromous species that occurs in both sides of the North Atlantic. The aims of this study were to analyse, using microsatellite markers, the genetic diversity and population structure of sea lamprey throughout its distributional range. Analyses demonstrated consistent signs of high population differentiation between European and North American samples (two-groups structure), most probably due to isolation by distance, but low differentiation among populations from the same coast. The apparent lack of homing in this species is in line with its high evolutive success, as homing may bring adults back to natal habitats that have changed, or that are intermittently unfavourable. Analyses also demonstrated higher levels of genetic diversity in North American samples; DIVERSIDADE GENÉTICA E ESTRUTURA POPULACIONAL DA LAMPREIA-MARINHA (PETROMYZON MARINUS L.) AO LONGO DA SUA ÁREA DE DISTRIBUIÇÃO Resumo: As lampreias são organismos ancestrais com cerca de 360 milhões de anos de existência. No decorrer da longa escala evolutiva têm vindo a adquirir adaptações aos locais que colonizaram, tendo uma forte capacidade evolutiva e adaptativa. A lampreia-marinha (Petromyzon marinus L.) é uma espécie parasita e anádroma que ocorre em ambas as costas do Atlântico Norte. Este estudo teve como principal objetivo estudar a diversidade genética e a estrutura populacional desta espécie ao longo da sua área de distribuição, através do uso de microssatélites. Os resultados demonstraram forte divergência entre populações das costas Este e Oeste do Atlântico Norte, provavelmente devido à elevada distância entre populações, mas pouca diferenciação entre populações da mesma costa. A ausência de homing nesta espécie terá contribuído para o seu sucesso evolutivo, uma vez que o homing pode levar indivíduos a reproduzirem-se em habitats que se tornaram desfavoráveis ou intermitentemente inapropriados. Os resultados demonstraram também uma maior variabilidade genética nas populações americanas.

Microbial diversity and metabolic potential in caves

Caves are dark and oligotrophic habitats where chemotrophic microbial communities interact with the inorganic mineral rocks and cooperate organizing themselves in complex biological formations, which are visible in caves as biofilms, biodeposits or biospeleothems. In these environments, microorganisms contribute to the turnover of the matter and activate peculiar enzymatic reactions leading to the modification of the mineral rocks and to the production of metabolites with possible industrial and pharmaceutical interest. In this PhD thesis, various molecular and geomicrobiological approaches were used to investigate the microbial diversity and potential activities in different cave systems, i.e. the orthoquartzite cave Imawarì Yeuta, the sufidic cave Fetida and the ice cave Cenote Abyss. This is aimed at gathering indications on the possible interactions that support microbial growth and its impact in cave environments. As a result, microbial taxa and functions associated to light-independent chemolithotroph and heterotrophic activities were identified in the three caves, indicating the involvement of microorganisms in i) silica mobilization and amorphization processes and the formation of a novel type of silica-based stromatolite in Imawarì Yeuta Cave, ii) the formation of three types of biofilm/biodeposit involved in sulphur cycle and in the speleogenesis of Fetida Cave, iii) the development of biofilms and their maintenance under psychrophilic conditions in samples collected from ice in Cenote Abyss. Additionally, the metabolic potentials of around one hundred isolates derived from these cave systems were evaluated in terms on anti-microbial activity. The results pointed out that unexplored and oligotrophic caves are promising environments for novel bioactive molecules discovery.

Irrigation and drainage canals role for plant diversity and nature conservation

Aims: With this research, we wanted to investigate and promote the conservation of biodiversity in the network of drainage canals of the Po Valley Study area: The canal network of Bologna plain, long more than 1150 km (Po Valley, North Italy) Methods: In Chapter II we analyzed the geographical patterns that characterize our transects, the land use of their upstream basins, the water quality at the closure points of their river basins. In Chapter III we described the plant communities with some ecological information and we also tested the effect of the canal size on the plant communities. In Chapter IV we described the relation beetween some functional traits of the plant species sampled and some environmental parameters Results: A total of 272 species were sampled in 118 transects. The plant communities of the drainage canals have been found to have a significant influence: the geographical pattern "proximity to protected areas", the class of land use "agrozootechnical settlements", and some water parameters. The analysis of the parameter "canal depth" indicated a significant distinction between small and large canals based on plant communities. The functional composition of the plant communities was affected by the bank aspect, the inclusion/exclusion from the protected areas and the upstream basin land uses. Moreover, the functional groups of species responded differently to environmental drivers, water quality gradients and were influenced by a combination of environmental stresses Conclusions: This research confirms the key role of the canals network in sustaining the plant richness in oversimplified landscapes. Considering the fragility of the floodplains and the global warming that is taking place, it is necessary to rethink the role of irrigation canals and their plant communities in the near future. This work reinforces the belief that long-term sampling plans and greater knowledge about canal management practices are needed

Applied genomics to monitor the genetic diversity and integrity of honey bee (Apis mellifera) populations and define sustainable strategies to benefit the beekeeping sector

Honey bees are considered keystone species in ecosystem, the effect of harmful pesticides for the honey bees, the action of extreme climatic waves and their consequence on honey bees health can cause the loss of many colonies which could contribute to the reduction of the effective population size and incentive the use of non-autochthonous queens to replace dead colonies. Over the last decades, the use of non-ligustica bee subspecies in Italy has increased and together with the mentioned phenomena exposed native honey bees to hybridization, laeding to a dramatic loss of genetic erosion and admixture. Healthy genetic diversity within honey bee populations is critical to provide tolerance and resistance to current and future threatening. Nowadays it is urgent to design strategies for the conservation of local subspecies and their valorisation on a productive scale. In this Thesis we applied genomics tool for the analysis of the genetic diversity and the genomic integrity of honey bee populations in Italy are described. In this work mtDNA based methods are presented using honey bee DNA or honey eDNA as source of information of the genetic diversity of A. mellifera at different level. Taken together, the results derived from these studies should enlarge the knowledge of the genetic diversity and integrity of the honey bee populations in Italy, filling the gap of information necessary to design efficient conservation programmes. Furthermore, the methods presented in these works will provide a tool for the honey authentication to sustain and valorise beekeeping products and sector against frauds.

Assessing the relationship between bee/flower diversity and vegetation structural heterogeneity through UAV photogrammetry

The ecosystem services provided by bees are very important. Factors as habitat fragmentation, intensive agriculture and climate change are contributing to the decline of bee populations. The use of remote sensing could be a useful tool for the recognition of sites with a high diversity, before performing a more expensive survey in the field. In this study the ability of Unmanned Aerial Vehicles (UAV) images to estimate biodiversity at local scale has been analysed testing the concept of the Height Variation Hypothesis (HVH). This approach states that, the higher the vegetation height heterogeneity (HH) measured by remote sensing information, the higher the vertical complexity and the higher vegetation species diversity. In this thesis the concept has been brought to a higher level, in order to understand if the vegetation HH can be considered a proxy also of bee species diversity and abundance. We tested this approach collecting field data on bees/flowers and RGB images through an UAV campaign in 30 grasslands in the South of the Netherlands. The Canopy Height Model (CHM) were derived through the photogrammetry technique "Structure from Motion" (SfM) with resolutions of 10cm, 25cm, 50cm. Successively, the HH assessed on the CHM using the Rao's Q heterogeneity index was correlated to the field data (bee abundance, diversity and bee/flower species richness). The correlations were all positive and significant. The highest R2 values were found when the HH was calculated at 10cm and correlated to bee species richness (R2 = 0.41) and Shannon’s H index (R2 = 0.38). Using a lower spatial resolution the goodness of fit slightly decreases. For flower species richness the R2 ranged between 0.36 to 0.39. Our results suggest that methods based on the concept behind the HVH, in this case deriving information of HH from UAV data, can be developed into valuable tools for large-scale, standardized and cost-effective monitoring of flower diversity and of the habitat quality for bees.

Eurooppalainen näkökulma suomalaiseen kulttuurijohtamiseen ja suomalaiseen yhteiskuntaan sopeutuminen

Tässä tutkielmassa korostuu Euroopan sisäinen kulttuurillinen erilaisuus ja sen vaatimukset Suomessa toimiville monikansallisille organisaatioille. Eurooppalaista johtamista on tutkittu jo vuosikymmenten ajan, mutta erityisesti suomalaisesta, Suomessa työskentelevien eurooppalaisten näkökulmasta ei vastaavaa tutkimusta tiettävästi ole aiemmin tehty. Tutkimuksen tavoitteena on selvittää suomalaisen kulttuurin ja johtajuuden arvoeroja muiden Euroopan Unionin maiden kansalaisten näkökulmasta. Pohjois-Euroopan maista tutkimuksessa on mukana edustajia Ruotsista, Tanskasta ja Hollannista. Keski-Euroopan maista edustusta löytyy Saksasta ja Itävallasta, kun Etelä-Eurooppaa edustavat ranskalaiset ja italialaiset henkilöt. Itäisestä Euroopasta mukana on latvialaisia henkilöitä ja anglo-Euroopasta britannialaisia. Tutkimus kartoittaa tutkimukseen mukaan otettujen maiden yhteisö- ja johtamiskulttuurillisia törmäyskohtia suomalaisen kulttuurin kanssa, jotta Suomessa toimivat monikansalliset työyhteisöt löytäisivät yhä parempia strategioita eurooppalaisen ihmispääoman, eurooppalaisten työntekijöiden ja näiden osaamisen, johtamiseksi ja monikansallisen yhteisön toimivuuden ymmärtämiseksi ja parantamiseksi. Näitä törmäyskohtia tarkastellaan empiirisesti Hofsteden (1984) kulttuuridimensioiden, Housen ym. (1999) GLOBE-tutkimuksen ja Koopmanin ym. (1999) johtajuusulottuvuuksien avulla. Tutkimusaineisto koostuu Internet-pohjaisesta kyselystä, joka suoritettiin keväällä 2010. Siihen osallistui 75 Suomessa työskentelevää Euroopan Unionin kansalaista. Analysointimenetelmänä tutkimuksessa käytetään klusterointia, keskiarvoistuksia, Kruskall Wallisin testiä ja sisällönanalyysiä tukemaan määrällisen analyysin tuloksia ja tuomaan esiin siitä poikkeavia havaintoja. Tutkimuksen ytimessä on johtamiskulttuurilliset erot, joista tehdään laajempaa analyysiä edellä mainituin keinoin. Yhteisökulttuurillisia eroja havainnoidaan tutkimuskyselyyn perustuvien väittämien perusteella tehtyjen histogrammien avulla. Tutkimuksen tulokset osoittavat, että maantieteellinen, uskonnollinen tai poliittinen läheisyys eivät määritä kulttuurillista läheisyyttä tai johda kulttuurilliseen sopeutumiseen. Poliittinen raja kahden naapurin välillä saattaa johtaa saman asian kokemiseen kahdella täysin eri tavalla. Lisäksi tuloksista käy ilmi, että yhteisökulttuurilliset ja johtamiskulttuurilliset arvot eivät välttämättä kulje käsikkäin Suomessa työskentelevien eurooppalaisten näkökulmasta. Yleisinä kulttuurillisina kipukohtina tutkimuksessa nousee esiin suomalaisen kulttuurin sosiaalisuuden ja kommunikaation puutteellisuus, toisaalta suomalaiseen kulttuuriin sopeutumista helpottaa suomalainen rehellisyys ja suoruus. Johtamiskulttuurillisina haasteina eurooppalaiset kokevat suomalaisen tyyppijohtajan kyvyttömyyden riskien ottamiseen ja kannustusmenetelmien puutteet. Tämän lisäksi tutkimuksessa tulee ilmi useita tiettyihin kansallisuuksiin liittyviä törmäyskohtia. Tutkimus on nähtävä tämän hetken kuvauksena. Kulttuuritutkimus sisältää monia tekijöitä, jotka ovat jatkuvassa muutoksessa ja lisäksi hyvin subjektiivisia. Kulttuurijohtajuus merkitsee kuitenkin nyt ja tulevaisuudessa kykyä olla innovatiivinen, joustava ja herkkä sosiaalisille merkityksille.