Effects of Ischemia and Reperfusion on P2X(2) Receptor Expressing Neurons of the Rat Ileum Enteric Nervous System

Purpose We investigated the effects of ischemia/reperfusion in the intestine (I/R-i) on purine receptor P2X(2)-immunoreactive (IR) neurons of the rat ileum. Methods The superior mesenteric artery was occluded for 45 min with an atraumatic vascular clamp and animals were sacrificed 4 h later. Neurons of the myenteric and submucosal plexuses were evaluated for immunoreactivity against the P2X(2) receptor, nitric oxide synthase (NOS), choline acetyl transferase (ChAT), calbindin, and calretinin. Results Following I/R-i, we observed a decrease in P2X(2) receptor immunoreactivity in the cytoplasm and surface membranes of neurons of the myenteric and submucosal plexuses. These studies also revealed an absence of calbindin-positive neurons in the I/R-i group. In addition, the colocalization of the P2X(2) receptor with NOS, ChAT, and calretinin immunoreactivity in the myenteric plexus was decreased following I/R-i. Likewise, the colocalization between P2X(2) and calretinin in neurons of the submucosal plexus was also reduced. In the I/R-i group, there was a 55.8% decrease in the density of neurons immunoreactive (IR) for the P2X(2) receptor, a 26.4% reduction in NOS-IR neuron, a 25% reduction in ChAT-IR neuron, and a 47% reduction in calretinin-IR neuron. The density of P2X(2) receptor and calretinin-IR neurons also decreased in the submucosal plexus of the I/R-i group. In the myenteric plexus, P2X(2)-IR, NOS-IR, ChAT-IR and calretinin-IR neurons were reduced in size by 50%, 49.7%, 42%, and 33%, respectively, in the I/R-i group; in the submucosal plexus, P2X(2)-IR and calretinin-IR neurons were reduced in size by 56% and 72.6%, respectively. Conclusions These data demonstrate that ischemia/reperfusion of the intestine affects the expression of the P2X(2) receptor in neurons of the myenteric and submucosal plexus, as well as density and size of neurons in this population. Our findings indicate that I/R-i induces changes in P2X(2)-IR enteric neurons that could result in alterations in intestinal motility.

POLYGLYCOLIC ACID TUBE ASSOCIATED WITH GM1 IN REGENERATION OF PERIPHERAL NERVES

Introduction: Nerve allografting is regarded as a treatment of choice in large neural tissue losses preventing repair by primary anastomosis. In these cases, a synthetic polyglycolic acid tube is an alternative for nerve grafting. On the other hand, several studies have emphasized the importance of neurotrophic factors on neural regeneration, including substances with potential to optimize neural regeneration, especially the GM1, an neurotrophic enhancer factor. Objective: to compare, in rats, the neural regeneration degree using histological analysis, regenerated myelinized axons count, and functional analysis with the use of neurotube and GM1. Methods: This assessment was performed by interposing allograft (group A), polyglycolic acid tube (group B) and polyglycolic acid tube associated to GM1 (group C) on 5-mm sciatic nerve defects. Results: Neuroma formation was found only on group A. Groups A and C showed similar histological patterns, except for the regenerated axons on group C, which were shown to be better organized and myelinized than in group A. Conclusion: on functional recovery, no statistically significant difference was found for the three groups, despite of qualitative and quantitative histological differences found.

Depot-specific modulation of adipokine levels in rat adipose tissue by diet-induced obesity: The effect of aerobic training and energy restriction

The present study examined the effects of aerobic training and energy restriction on adipokines levels in mesenteric (MEAT) and retroperitoneal (RPAT) white adipose tissue from obese rats. Male Wistar rats were fed with standard laboratory diet (Control group) or high fat diet (HFD). After 15 weeks, HFD rats were randomly assigned to the following groups: rats submitted to HFD, which were sedentary (sedentary HFD, n = 8) or trained (trained HFD, n = 8); or submitted to energy-restriction (ER), which were sedentary (sedentary ER, n = 8) or trained (trained ER, n = 8). Trained rats ran on a treadmill at 55% VO(2max) for 60 min/day, 5 days/week, for 10 weeks. ER rats were submitted to a reduction of 20% daily caloric ingestion compared to the Control group. ER and aerobic training decreased body weight, MEAT and RPAT absolute weight, and fat mass. IL-6, IL-10 and TNF-alpha levels were decreased and adiponectin did not change in RPAT in response to ER protocol. On the other hand, ER and the aerobic training protocol decreased IL-6, TNF-alpha and adiponectin levels in MEAT. Absolute MEAT weight showed a positive correlation with IL-6 (r = 0.464), INF-alpha (r = 0.508); and adiponectin (r = 0.342). These results suggest a tissue-specific heterogeneous response in adipokines level. The combination of the protocols (aerobic training and energy restriction) did not induce an enhanced effect. Published by Elsevier Ltd.

Determination of controls on Holocene barrier progradation through application of OSL dating: The Ilha Comprida Barrier example, Southeastern Brazil

Barrier development during the Holocene is studied using the example of the Ilha Comprida, Southeastern Brazil. Aerial photos, facies analysis, and optically stimulated luminescence dating are used to define the barrier emergence and evolution. Optically stimulated luminescence ages and facies successions indicate that the Ilha Comprida probably began as a Holocene transgressive barrier island 6000 years ago, just before the last relative sea-level maximum. Since then the barrier has progradated through the addition of curved beach ridges. Based on beach ridge alignments, six units of growth are identified with two growth directions, transverse and longitudinal. Rates of progradation with transverse growth vary from 0.13 to 4.6 m/year. Rates of longitudinal growth to NE range from 5.2 to 30 m/year. Variation in coastal progradation rates and sediment retention during the last 6000 years is compared with climate, physiography and relative sea-level changes. The physiography, represented by pre-Cenozoic hills, is the major control on sediment retention and alternation between longitudinal and transverse growth. Climate variations, such as the Little Ice Age event, apparently control the formation of ridges types: beach ridges, foredunes, and blowouts. These results allow the use of the Ilha Comprida Barrier as an example to analyze the major controls on barriers progradation. (C) 2011 Elsevier B.V. All rights reserved.

Modeling worldwide highway networks

This Letter addresses the problem of modeling the highway systems of different countries by using complex networks formalism. More specifically, we compare two traditional geographical models with a modified geometrical network model where paths, rather than edges, are incorporated at each step between the origin and the destination vertices. Optimal configurations of parameters are obtained for each model and used for the comparison. The highway networks of Australia, Brazil, India, and Romania are considered and shown to be properly modeled by the modified geographical model. (C) 2009 Elsevier B.V. All rights reserved.

Border trees of complex networks

The comprehensive characterization of the structure of complex networks is essential to understand the dynamical processes which guide their evolution. The discovery of the scale-free distribution and the small-world properties of real networks were fundamental to stimulate more realistic models and to understand important dynamical processes related to network growth. However, the properties of the network borders (nodes with degree equal to 1), one of its most fragile parts, remained little investigated and understood. The border nodes may be involved in the evolution of structures such as geographical networks. Here we analyze the border trees of complex networks, which are defined as the subgraphs without cycles connected to the remainder of the network (containing cycles) and terminating into border nodes. In addition to describing an algorithm for identification of such tree subgraphs, we also consider how their topological properties can be quantified in terms of their depth and number of leaves. We investigate the properties of border trees for several theoretical models as well as real-world networks. Among the obtained results, we found that more than half of the nodes of some real-world networks belong to the border trees. A power-law with cut-off was observed for the distribution of the depth and number of leaves of the border trees. An analysis of the local role of the nodes in the border trees was also performed.

Inferential Implications of Over-Parametrization: A Case Study in Incomplete Categorical Data

P>In the context of either Bayesian or classical sensitivity analyses of over-parametrized models for incomplete categorical data, it is well known that prior-dependence on posterior inferences of nonidentifiable parameters or that too parsimonious over-parametrized models may lead to erroneous conclusions. Nevertheless, some authors either pay no attention to which parameters are nonidentifiable or do not appropriately account for possible prior-dependence. We review the literature on this topic and consider simple examples to emphasize that in both inferential frameworks, the subjective components can influence results in nontrivial ways, irrespectively of the sample size. Specifically, we show that prior distributions commonly regarded as slightly informative or noninformative may actually be too informative for nonidentifiable parameters, and that the choice of over-parametrized models may drastically impact the results, suggesting that a careful examination of their effects should be considered before drawing conclusions.Resume Que ce soit dans un cadre Bayesien ou classique, il est bien connu que la surparametrisation, dans les modeles pour donnees categorielles incompletes, peut conduire a des conclusions erronees. Cependant, certains auteurs persistent a negliger les problemes lies a la presence de parametres non identifies. Nous passons en revue la litterature dans ce domaine, et considerons quelques exemples surparametres simples dans lesquels les elements subjectifs influencent de facon non negligeable les resultats, independamment de la taille des echantillons. Plus precisement, nous montrons comment des a priori consideres comme peu ou non-informatifs peuvent se reveler extremement informatifs en ce qui concerne les parametres non identifies, et que le recours a des modeles surparametres peut avoir sur les conclusions finales un impact considerable. Ceci suggere un examen tres attentif de l`impact potentiel des a priori.

Comparing diagnostic tests with missing data

When missing data occur in studies designed to compare the accuracy of diagnostic tests, a common, though naive, practice is to base the comparison of sensitivity, specificity, as well as of positive and negative predictive values on some subset of the data that fits into methods implemented in standard statistical packages. Such methods are usually valid only under the strong missing completely at random (MCAR) assumption and may generate biased and less precise estimates. We review some models that use the dependence structure of the completely observed cases to incorporate the information of the partially categorized observations into the analysis and show how they may be fitted via a two-stage hybrid process involving maximum likelihood in the first stage and weighted least squares in the second. We indicate how computational subroutines written in R may be used to fit the proposed models and illustrate the different analysis strategies with observational data collected to compare the accuracy of three distinct non-invasive diagnostic methods for endometriosis. The results indicate that even when the MCAR assumption is plausible, the naive partial analyses should be avoided.

Missing data mechanisms and their implications on the analysis of categorical data

We review some issues related to the implications of different missing data mechanisms on statistical inference for contingency tables and consider simulation studies to compare the results obtained under such models to those where the units with missing data are disregarded. We confirm that although, in general, analyses under the correct missing at random and missing completely at random models are more efficient even for small sample sizes, there are exceptions where they may not improve the results obtained by ignoring the partially classified data. We show that under the missing not at random (MNAR) model, estimates on the boundary of the parameter space as well as lack of identifiability of the parameters of saturated models may be associated with undesirable asymptotic properties of maximum likelihood estimators and likelihood ratio tests; even in standard cases the bias of the estimators may be low only for very large samples. We also show that the probability of a boundary solution obtained under the correct MNAR model may be large even for large samples and that, consequently, we may not always conclude that a MNAR model is misspecified because the estimate is on the boundary of the parameter space.

Estrutura de capitais : evidências do caso brasileiro

Trabalhos internacionais de caráter empírico têm se dedicado a investigar qual das vertentes teóricas acerca da estrutura de capitais melhor explica a prática corporativa, chegando freqüentemente à oposição entre as teorias de equilíbrio estático – quer baseado em aspectos fiscais, quer em custos de agência – e as calcadas em assimetria informacional. O presente trabalho, a partir de dados de empresas brasileiras listadas na Bolsa de Valores de São Paulo – BOVESPA, no período 1997-2002, utiliza-se de duas especificações distintas para modelos de regressão, cada qual mais adequada a uma das linhas teóricas citadas, com o intuito de apurar qual possui maior aderência à realidade brasileira e, com base nos resultados, que teoria possui maior força explicativa. Os resultados indicam, primeiramente, que a especificação baseada nas predições da hierarquização das fontes de financiamento apresenta um desempenho superior àquela mais voltada ao equilíbrio estático e, em segundo lugar, a análise dos resultados de ambas as especificações também é favorável à pecking order. Isto posto, conclui-se ser esta a teoria que melhor descreve o comportamento das empresas brasileiras em suas decisões sobre suas estruturas de capitais.

O processo político da reforma administrativa: o caso da gestão petista em São Paulo (1989-1992)

Trata do processo político da Reforma Administrativa na gestão petista em São Paulo, (1989-1992), analisando, tanto o processo de formulação da proposta, como os motivos que impediram a sua aprovação pela Câmara dos vereadores. O partido governante, constituido por principios contraditórios e ambiguos, através do Executivo, elabora uma proposta de Reforma Administrativa, sem conseguir conciliar os diferentes interesses envolvidos no processo de formulação da proposta. A mesma dificuldade é verificada no processo de tramitação do Projeto de lei na Cãmara Municipal. Considera-se nesse estudo a dificuldade dos governantes petistas em lidar com o processo de negociação política e o padrão de funcionamento do Legislativo na aprovação dos projetos de interesse do Executivo, como fatores determinantes do fracasso na aprovação do Projeto de Lei de Reforma Administrativa na Cãmara Municipal de São Paulo.

O projeto de integração competitiva: uma avaliação critica da politica industrial do governo Collor

O objetivo do trabalho é analisar de forma crítica e à luz das grandes transformações em curso nas relações econômicas internacionais, a chamada estratégia de integração competitiva, que vem orientando a formulação da nova política industrial brasileira, e em particular as Diretrizes Gerais de Política Industrial e de Comércio Exterior do Governo Collor, divulgaddas em junho de 1990

O ensino da ética em cursos de graduação em administração

Ao tratar de tema tão amplo e complexo como é a ética, buscou-se delimitar o seu foco de estudo no ensino da ética nos cursos de administração, e na análise da literatura existente. O estudo mostra um amplo panorama do desenvolvimento da ética até os dias de hoje. A origem e aplicação da ética nos negócios e o conceito de responsabilidade social, mostrando a importância da ética nos negócios. Através da análise de uma pesquisa sobre o tema, procurou-se mostrar o retrato ensino da ética em cursos de graduação em administração, e apresentar sugestões que contribuam para o desenvolvimento do tema.

Casos de fraudes corporativas financeiras: antecedentes, recursos substantivos e simbólicos relacionados

As pesquisas, no campo de estudos organizacionais, têm mostrado limitada capacidade para a compreensão e análise do complexo tema "fraudes corporativas", pois negligenciam o fenômeno como um processo (sequência de atos), tratando-o como um evento (ASHFORTH et al., 2008), menosprezando que cada tipo de fraude requer um conjunto de recursos, esquemas cognitivos e contextos (BAUCUS, 1994; MACLEAN, 2008; MISANGYI; WEAVER; ELMS, 2008). Eles não permitem responder à pergunta “Como a fraude ocorre nas corporações?”. Para tal é necessário identificar e analisar os fatores que permitem a fraude surgir e se sustentar. O presente trabalho, com intuito de identificar os elementos característicos do fenômeno da fraude e como se criou um contexto favorável para ela, estudou dois casos emblemáticos ocorridos no Brasil (Boi Gordo e Banco Santos). A análise, feita com uma abordagem de pesquisa baseada na grounded theory, com análise de documentos e de discursos, consubstanciou-se em um arcabouço teórico substantivo para o contexto de fraudes corporativas financeiras contra terceiros. O trabalho analisou o movimento do fenômeno, suas origens, seu desenvolvimento e consolidação e sua crise, identificando as variáveis antecedentes (de predisposição e oportunidade), bem como os recursos substantivos e simbólicos usados no desenvolvimento da fraude; evidenciando a forma como os recursos se relacionaram para criar a lógica institucional fraudulenta e; integrando os fatores antecedentes à operacionalização da fraude, de forma apreender os elementos constitutivos do processo. O esforço teórico e empírico aqui desenvolvido, resultou numa proposta inicial de modelo interpretativo, com a identificação de novos elementos teóricos. Evidenciou "o negócio baseado na confiança" como categoria central e mostrou de que modo esse elemento interage com outras categorias e propriedades. Entre os novos elementos estão os aspectos da vítima, a dialética centralização do comando e descentralização do negócio em várias empresas (com uso de "laranjas") e o uso de aspectos culturais como oportunidade para a fraude. Evidenciou ainda de que maneira os recursos substantivos usados por empresas idôneas ganharam novos significados, por meio de esquemas cognitivos e, permitiu a identificação de três momentos no processo: "origem", "pseudo sintonia" e "espiral". A dimensão multifacetada da fraude corporativa foi evidenciada, inclusive fazendo a ligação com a dimensão social (DEBORD, 1997; BOORSTIN, 1992; GOFFMAN, 1959; BAUDRILLARD, 1991; BOURDIEU, 2007). A tese constitui uma contribuição para a compreensão ampliada do fenômeno, numa perspectiva integrativa, interacionista e processual, que pode ser replicada em futuras pesquisas para construir um arcabouço robusto e generalizável em outros contextos substantivos ou ainda para a teoria formal de fraudes corporativas.

Um estudo sobre a autonomia da universidade brasileira

Este trabalho se propõe a estudar uma das características que sempre faz parte da natureza de qualquer Universidade: sua autonomia. Esta prerrogativa não apenas tem sido reconhecida como necessária para a atuação de uma Universidade na história de cada sociedade, como também é considerada o sinal mais preciso da presença da "idéia" de Universidade em uma Instituição de Ensino Superior.