毛壳菌产抗真菌活性物质菌株筛选及种间原生质体融合研究

毛壳菌属很多种类具有重要生防价值，其生防机理包括对植物病原真菌的重寄生作用、诱导植物产生抗病性、产生抗真菌活性的次生代谢产物等。迄今，学界对毛壳菌的研究主要集中在毛壳菌的生防机理，毛壳菌活性次生代谢产物的分离等方面。本研究致力于产抗生素的毛壳菌的种间原生质体融合，从产抗生素毛壳菌菌株的筛选开始，进而对产抗生素的角毛壳菌进行诱变选育，最终用产不同抗生素的角毛壳菌与球毛壳菌进行种间原生质体融合。主要有以下五方面研究结果。 1、毛壳菌抗真菌活性物质产生菌株的筛选：不同毛壳菌菌株发酵液采用琼脂扩散法对植物病原真菌进行抑菌活性试验，结果显示，菌株CH08和CH23的发酵液对芒果炭疽、苹果炭疽和马铃薯晚疫菌具有抑制作用。菌株CH16和CH17的发酵液对芒果炭疽菌、苹果炭疽菌有抑制作用。菌株CH21发酵液对辣椒炭疽菌和西瓜枯萎菌有抑制作用。经形态学研究，菌株CH08、CH16、CH17和CH23鉴定为球毛壳菌，菌株CH21鉴定为角毛壳菌。对角毛壳菌与球毛壳菌菌株发酵液抑菌谱比较，发现角毛壳菌与球毛壳菌发酵液具有明显不同的抑菌谱，表明角毛壳菌与球毛壳菌产生不同的抗真菌活性物质。 2、角毛壳菌（CH21）和球毛壳菌（CH08）原生质体制备和再生条件研究：考察了菌龄、酶浓度、稳渗剂及其浓度、酶解温度、酶解时间及再生培养基对原生质体制备和再生的影响。用菌龄为生长54 h的角毛壳菌菌丝，以0.06 M磷酸缓冲液（pH6.0）配制成含蜗牛酶15 mg/ml、溶壁酶10 mg/ml、蔗糖0.6 mol/L的酶解液，30℃酶解1.5 h，原生质体释放量2.02×107个/g；以PDA为再生培养基，0.7 mol/L的蔗糖再生稳渗剂，再生率可达51.45%。用菌龄为生长48 h的球毛壳菌菌丝，以0.06 M磷酸缓冲液（pH6.0）配制成含蜗牛酶15 mg/ml、溶壁酶10 mg/ml、蔗糖0.6 mol/L的酶解液，30℃酶解1 h，原生质体释放量达1.57×108个/g；以PDA为再生培养基，0.7 mol/L的蔗糖为再生稳渗剂，再生率可达41.48%。 3、角毛壳菌（CH21）原生质体紫外诱变选育：以CH21为出发菌株，制备原生质体进行紫外诱变，诱变条件为：15 w紫外灯，距离30 cm，照射90 s，致死率80%~85%。建立了诱变菌株初筛的双层平板筛选模型。经平板初筛和摇瓶复筛，获得一株突变菌株CH21-I-402，其发酵液抑菌活性较出发菌株提高18.3%。 4、抗性标记菌株的获得：菌株CH21-I-402和CH08抗生素药敏试验表明， CH21-I-402菌株对潮霉素有抗性、对G418（Geneticin）敏感，菌株CH08对潮霉素和G418都敏感。根癌农杆菌EHA105介导的新霉素磷酸转移酶基因转化球毛壳菌，经PCR检测，新霉素磷酸转移酶基因成功转化进菌株CH08-GR70，CH08-GR120。转化子对G418抗性提高3～4倍，对潮霉素仍然比较敏感。 5、以G418和潮霉素抗性为筛选标记的原生质体融合与融合菌株AFLP分析：制备角毛壳菌CH21-I-402和球毛壳菌CH08-GR70原生质体，以35％的PEG6000为助融剂进行原生质体融合，以65 μg/ml的潮霉素和60 μg/ml G418为抗性筛选标记，获得46个再生菌株。再生菌株连续传代5代后，再生菌株表现出多种形态类型。利用AFLP技术对再生菌株及亲本菌株基因组DNA分析表明，再生菌株PF1、PF26为融合菌株。抑菌活性测试表明，融合菌株PF26发酵液对芒果炭疽菌和苹果轮纹菌有强的抑制作用，且抑菌活性比亲本球毛壳菌明显提高。 Chaetomium spp. have great potentials as biocontrol agents against a range of plant pathogens on the basis of its mycoparasitism, induced plant disease resistance, production of antifungal metabolites, and so on. Previous researches on C. spp. mostly focused on the mechanisms of its biocontrol and the isolation of secondary metabolites. In this study, screening antifungal C. spp., mutation breeding of C. cupreum and interspecies protoplast fusion between C. cupreum and C. globosum were carried out, respectively. The corresponding results are as follows: Firstly, among more than 40 C. spp., the strains produced anti-fungal antibiotics were screened by agar diffusion experiments. Results showed that both CH08 and CH23 had inhibition against Colletotrichum gloeosporioides, Cladosporium fulvum, and Phytophthora infestans. Both CH16 and CH17 had inhibition against Colletotrichum gloeosporioides and Cladosporium fulvum. In addition, CH21 exhibited anti-fungal activity against Fusarium oxysporum f. sp niveum and Colletotrichum capsici. Furthermore, CH08, CH16, CH17 and CH23 were identified as C. globosum, CH21 was proved to be C. cupreum based on morphology. The comparison of the anti-fungal spectrum between C. cupreum and C. globosum, showed they could produce different antibiotics. Secondly, specified protocols for preparing and regenerating protoplasts from mycelia of C. cupreum CH21 and C. globosum CH08 were studied. The effects of the age mycelia, the concentration of enzyme, digestion temperature and time, kinds of osmotic stabilizer and regeneration medium on protoplasts preparation and regeneration were all optimized, respectively. In one protocol, with 15 mg/mL snailase, 10 mg/mL lywallzyme, 0.6 M sucrose, in 0.06 M phosphate buffer (pH6.0), and digested for 1.5 h at 30 ºC, 2.02×107 protoplasts from each gram mycelia were obtained from cultures of C. cupreum CH21 grown in potato dextrose broth (PDB) medium for 54 h. And when 0.7 M sucrose was used as osmotic stabilizer in the regeneration medium OPDA (potato dextrose agar with osmotic stabilize), the regeneration efficiency of protoplasts was 51.45%. In another protocol, with 15 mg/mL snailase, 10 mg/mL lywallzyme, 0.6 M sucrose, in 0.06 M phosphate buffer (pH6.0), and digested for 1 h at 30 ºC, 1.57×108 protoplasts from each gram mycelia were obtained from cultures of C. globosum CH08 grown in PDB for 48 h. And when 0.7 M sucrose was used as osmotic stabilizer in the regeneration medium OPDA, the regeneration efficiency of protoplasts was 41.48%. Thirdly, the mutagenesis conditions and secondary screening model of C. cupreum CH21 were explored. An 80% to 85% death rate could be achieved when the protoplasts of C. cupreum CH21 were irradiated by 15 w UV lamp from 30 cm distance for 90 s. In addition, the doublelayer plate’s method for the primary screening of high-producing antibiotics strains was established. A high yielding antibiotic mutant CH21-I-402 was obtained through the primary screening on plate and the secondary selection in Erlenmeyer flask, compared to the original CH21 strain, the antifungal activity of the mutant CH21-I-402 was increased by 18.3%. Fourth, the sensitivity to antibiotics of both C. cupreum CH21-I-402 and C. globusm CH08 was detected. Results showed C. cupreum CH21-I-402 was sensitive to G418 (Geneticin) (Gs) and resistant to Hygromycin B(Hr), and C. globusm CH08 was sensitive to both G418 (Geneticin) (Gs) and Hygromycin B(Hs). At the same time, neomycin phosphotransferase II (npt II) gene was transformed into C. globusm CH08(Gs, Hs) mediated by Agrobacterium tumefaciens EHA105, and the npt II gene was verified by polymerase chain reaction in resistance to G418 strains CH08-GR70 and CH08-GR120. The transformants still showed sensitive to Hygromycin B(Hs). Finally, a selection system for hybrids was set up by interspecies protoplast fusion between C. cupreum and C. globusm using dominant selective drug resistance markers. At first, protoplasts of C. cupreum CH21-I-402 (Hr, Gs) and C. globusm CH08-GR70 (Hs, Gr) were prepared, then the protoplasts were fused in the presence of 35% polyethylene glycol 6000 and regenerated on OPDA medium with 65 μg/ml Hygromycin B and 60μg/ml G418, at last 46 colonies with Hr and Gr were obtained. Even after 5 generations’ subculture, most of the colonies displayed significant difference in taxonomic characteristics with their parental strains. Regenerated strains PF1 and PF26 were confirmed as fusants by amplified fragment length polymorphisms analysis with the genomic DNA as the model. PF26 showed higher inhibitory activity against Colletotrichum gloeosporioides and Macrophoma kuwatsukai than that of the parental strain C. globusm.

次级束流线设备FRS和现有RIBLL2的优化

本文工作分为两个部分：第一部分是设计德国重离子研究中心（GSI）的一段新的次级束流线；第二部分计算了在建的R工已LLZ对裂变反应碎片的传输分离，同时为提高分离本领对RIBLLZ进行了优化计算和设计。FRS是德国重离子研究中心（GSI）的次级束流线。其分支FRS-CAVEB的终端是川JAD工N的实验终端，这条线的束流品质不理想（传输率只有0．1％），因此GSI拟将AI'AD州设备（原在CAVEB）移到CAVEC中，形成FRS一CAVEC束流线，又称FRS-R3Bcave。本文对FRS-CoveC进行了束流光学设计和计算，同时模拟了FRS-CAVEC对弹核碎裂反应的传输分离。新束线按计算结果安装了一个二极磁铁和3个四极磁铁，并通过了实验测试，测试结果表明：（1）系统具有良好的消色散特性，即系统在FRS出口和实验终端处达到了消色差。（2）系统的传输率基本符合理论计算值，相对CAVEB分支传输率提高了一个量级以上。当不放任何阻止物质在束流线上时，Ar主束传输率可以达到100％；当放置3mm厚的Sdntillator探测器在S2，S8，CaveC处时，主束传输率基本和理论计算值相近（640k-计算值，610k-实验值）。对比FRS-C八VEB的实验传输率（0．1％），可知通过改进，FRS-C八VEC的传输率将得到极大的改善。RIBLLZ是连接CSR两环之间的次级束流线。现有的RIBLLZ束流线设计的比较紧凑，立体角接收度大，使得所有粒子都可通过系统，可以作为零度质谱仪，尤其是可以将裂变产生的奇异核素注入到实验环进行质量测量。本文模拟了弹核碎片和裂变反应产生的次级束在现有的RIBLLZ上的传输情况。计算表明，现有的RIBLLZ对通过u裂变产生的大量较重奇异核素，具有很高的传输率。另一方面，现有的RIB七LZ在散焦面只有两个四极磁铁，为达到系统的消色差及较大的分辨率会使得Y方向的束流控制困难。基于此，对现有的R咸喝锻蓄尔＼一些改进计算。还理论计算比较了两个系统对裂变反应的传输分离情况。玺认算试知飞（1）优化后的RIBLLZ系统对束流在Y方向的控制将更灵活。（2）对裂变反应产物的传输，除了作为零度质谱仪外还可以作为单一核素的分离器。（3浑税盯确集磁系统的传输率对降能器厚度敏感度降低，对于u裂变反应产生的132sn的传输率，当降能器厚度从19／cmZ变化到39／clnu时，传输率下降20％，而未优化的RIBLLZ系统的传输率将下降90%。

Study of electron transfer across the liquid/ice-like matrix interface by scanning electrochemical microscopy

In this work, we report the findings of a study on scanning electrochemical microscopy (SECM) to investigate the interfacial electron-transfer (ET) reaction between the 7,7,8,8-tetracyanoquinodimethane radical anion (TCNQ(.-)) in 1,2-dichloroethane and ferricyanide in an ice-like matrix (a mixture of insulting ice and conductive liquid) under low temperatures. Experimental results indicate that the formed liquid/ice-like matrix interface is superficially similar in electrochemical characteristics to a liquid/liquid interface at temperatures above -20 degreesC. Furthermore, imaging data show that the surface of the ice-like matrix is microscopically flat and physically stable and can be applied as either a conductive or an insulting substrate for SECM studies. Perchlorate ion was selected as the common ion in both phases, the concentrations of which controlled the interfacial potential difference. The effect of perchlorate concentration in the DCE phase on interfacial reactions has been studied in detail. The apparent heterogeneous rate constants for TCNQ(.-) oxidation by Fe(CN)(6)(3-) in another phase under different temperatures have been calculated by a best-fit analysis, where the experimental approach curves are compared with the theoretically derived relationships. Reaction rate data obey Butler-Volmer formulation before and after the freezing point, which is similar to most other known cases of ET reactions at liquid/liquid interfaces. However, there is a sharp change observed for heterogeneous rate constants around the freezing point of the aqueous phase, which reflects the phase transition. At temperatures below -20 degreesC, surface-confined voltammograms for the reduction of ferricyanide were obtained, and the ice-like matrix became an insulating one, which indicates that the aqueous phase is really a frozen phase.

7500aBP以来冲绳海槽北部海洋古环境的演化特征

本文采用冲绳海槽北部柱状岩芯DOC024，通过对其微体古生物分析和地球化学分析，结合浮游有孔虫氧、碳同位素数据、AMS14C 测年结果和已有的研究资料，对7500年以来该区域古生产力演化、古上升流演化及黑潮对该区域的影响进行了分析探讨。 冲绳海槽北部，九州西南海区，全新世晚期7.5ka BP以来，该区的钙质和硅质生物生产力存在着显著的差异，钙质生物的生产量高值出现在7.0ka BP 左右，但在7.0-6.4ka BP 之后明显下降而且十分稳定；而3.0ka BP 左右是硅质生物生产量的高值期，3.0ka BP 之前硅质生物生产力逐渐增加，之后呈下降趋势；表层海水初级生产力亦在7.0ka BP 左右出现短暂的高值，之后呈下降趋势，波动较频繁，0.9kaBP前后也出现了较明显的高值。古生产力的这一变化过程可能主要反映了黑潮与陆架水混合过程，以及与其相关的上升流强度的变动。 冲绳海槽北部、九州西南海区，全新世7.5-7.0ka BP陆源物质影响明显， 7.0kaBP前后黑潮侵入并与陆架水强烈混合，7.0-6.0ka BP 期间黑潮影响逐渐控制了该区，上升流开始发生，温跃层开始变浅；约在6.0-2.0ka BP 之间上升流持续稳定发育，温跃层深度逐渐下降：6.0-5.0ka BP上升流影响作用于温跃层但对表层影响较小，5.0-4.0ka BP海水表层温度受上升流冷水影响降低；而约2.0-1.2ka BP，上升流减弱，海水表层温度回升；近1200 年来上升流加强、温跃层深度减小的趋势更加显著。上升流的发育明显地控制着温跃层深度的变化，上升流强度与黑潮强度密切相关，且在一定范围内有线性关系，冲绳海槽北部冷涡区域附近海洋环境的变化也存在着明显的区域性特征。 由于受冲绳海槽北部复杂的海洋环境、DOC024 柱状样中7.5ka BP 左右火山活动的扰动作用，使我们难以准确而详细地探讨全新世黑潮在冲绳海槽北部的变动；但根据对数据的分析讨论，并结合已有研究成果可以得出以下认识：在冲绳海槽北部，7.5aBP前后黑潮强度减弱，以至于几乎完全失去了对该海区的影响；7.0-2.0kaBP上升流的稳定发育说明了黑潮较稳定，仅有小幅波动；2.0-1.2kaBP上升流减弱，黑潮减弱；1.2kaBP伴随着气候的变暖，黑潮增强且幅度较大；0.3kaBP 随着气候变冷，黑潮有所减弱；总体上自7.0aBP前后黑潮入侵以来黑潮对该区的影响一直存在，而6.6kaBP以来黑潮底层水团对该区的影响比较稳定。

Morphological and genetic study of Prorocentrum donghaiense Lu from the East China Sea, and comparison with some related Prorocentrum species

In this paper, the detailed morphology of Prorocentrum donghaiense Lu from both field samples and cultures was examined, and a taxonomic comparison was made between P donghaiense and some related Prorocentrum spp. using morphological and molecular data and other published information. There were distinct differences among these species in morphological characteristics that historically have been presented as conservative features. The discrepancies extended beyond that of individual variations within the same species due to environmental factors. Therefore, these morphological features may not be conservative but, rather, polymorphic depending on environmental conditions. Based on this analysis, we suggest that the high-biomass bloom-forming species in the East China Sea, previously reported as Prorocentrum dentatum Stein, is P donghaiense Lu. The species reported from the East China Sea and Japanese and Korean waters appear to be the same species. Molecular data also suggest that P. dentatum (CCMP1517) and P. donghaiense are genetically identical. Therefore, the geographic distribution of P. donghaiense may be much wider than expected. (C) 2004 Elsevier B.V. All rights reserved.

小型无人直升机实验平台的设计与实现

高级控制算法对于无人直升机实现高机动性非常重要,但是在实现上也经常受制于鲁棒性和实时性的要求。同时这些控制方法在飞行测试时也会带来高风险。本文介绍了一种自制的安装在机械臂上的直升机作为实验平台去验证控制方法的有效性和可行性,这个平台还能够保证飞行测试的安全性。实验结果显示,能够实现转速跟踪控制以及航向动力学的辨识,并证明平台的有效性。

晚中新世以来中国北方风成沉积的磁性地层学和沉积学研究

The past two decades have witnessed an unprecedented growth of interest in the palaeoenvironmental significance of the Pleistocene loess deposits in northern China. However, it is only several years ago that the Tertiary red clay sequence underlying Pleistocene loess attracted much attention. One of the major advances in recent studies of eolian deposits on the Loess Plateau is the verification of the eolian origin for the Tertiary red clay sediments. The evidence of the eolian origin for the red clay is mainly from geochemical and sedimentological studies. However, sedimentological studies of the red clay deposits are still few compared with those of the overlying loess sediments. To date, the red clay sections located near Xifeng, Baoji, Lantian, Jiaxian, and Lingtai have been studied, with an emphasis on magnetostratigraphy. These sections have a basal age ranging from ～4.3 Ma to ～7.0 Ma. The thickness of the sections varies significantly, depending perhaps on the development of local geomorphological conditions and the drainage system. Although the stratigraphy of the red clay sections has been recorded in some detail, correlation of the red clay sequences has not yet been undertaken. Geological records (Sun J. et al., 1998) have shown that during glacial periods of the Quaternary the deserts in northem China were greatly expanded compared with modern desert distribution. During interglacial periods, desert areas contracted and retreated mostly to northwestern China because of the increase in inland penetration of monsoonal precipitation. According to pedogenic characteristics of the red clay deposits, the climatic conditions of the Loess Plateau is warmer and wetter generally in the Neogene than in the late Pleistocene. Panicle analyses show that grain size distribution of the red clay sequence is similar to that of the paleosols in the Pleistocene loess record, thus implying a relatively remote provenance of the red clay materials. However, the quantitative or semiquantitative estimates of the distance from the source region to the Loess Plateau during the red clay development remains to be investigated. In this study, magnetostratigraphic and sedimentological studies are conducted at two thick red clay sequences-Jingchuan and Lingtai section. The objectives of these studies are focused on further sedimentological evidence for the eolian origin of the red clay, correlation of red clay sequences, provenance of the red clay, and the palaeoclimate reconstruction in the Neogene. Paleomagnetic studies show that the Jingchuan red clay has a basal age of 8.0 Ma, which is 1 million years older than the previously studied Lingtai section. The Lingtai red clay sequence was divided into five units on the basis of pedogenica characteristics (Ding et al., 1999a). The Jingchuan red clay sequence, however, can be lithologically divided into six units according to field observations. The upper five units of the Jingchuan red clay can generally correlate well with the five units of the Lingtai red clay. Comparison of magnetic susceptibility and color reflectance records of four red clay sections suggests that the Lingtai red clay sequence can be the type-section of the Neogene red clay deposits in northern China. Pleistocene loess and modem dust deposits have a unimodal grain-size distribution. The red clay sediments at Jingchuan and Lingtai also have a unimodal grain-size distribution especially similar to the paleosols in the Pleistocene loess record. Sedimentological studies of a north-south transect of loess deposits above S2 on the Loess Plateau show that loess deposits had distinct temporal and spatial sedimentary differentiation. The characteristics of such sedimentary differentiation can be well presented in a triangular diagram of normalized median grain size, normalized skewness, and normalized kurtosis. The triangular diagrams of the red clay-loess sequence at Lingtai and Jingchuan indicate that loess-paleosol-red clay may be transported and sorted by the same agent wind, thus extending the eolian record in the Loess Plateau from 2.6 Ma back to about 8.0 Ma. It has been recognized that during the last glacial maximum (LGM) the deserts in northern China had a distribution similar to the present, whereas during the Holocene Optimum the deserts retreated to the area west of the Helan Mountains. Advance-retreat cycles of the deserts will lead to changes in the distance of the Loess Plateau to the dust source regions, thereby controlling changes in grain size of the loess deposited in a specific site. To observe spatial changes in sedimentological characteristics of loess during the last glacial-interglacial cycle, the texture of loess was measured along the north-south transect of the Loess Plateau. Since the southern margin of the Mu Us desert during the LGM is already known, several models of grain size parameters versus the minimum distance from the source region to depositional areas were developed. According to these semiquantitative models, the minimum distance from the source region to Lingtai and Jingchuan areas is about 600 km during the Neogene. Therefore the estimated provenance of the Tertiary red clay deposits is the areas now occupied by the Badain Jaran desert and arid regions west of it. The ratio of the free iron to total iron concentration attests to being a good proxy indicator for the summer monsoon evolution. The Lingtai Fe_20_3 ratio record shows high values over three time intervals: 4.8-4.1 Ma, 3.4-2.6 Ma, and during the interglacial periods of the past 0.5 Ma. The increase in summer monsoon intensity over the three intervals also coincides with the well-developed soil characteristics. It is therefore concluded that the East-Asia summer monsoon has experienced a non-linear evolution since the late Miocene. In general, the East Asia summer monsoon was stronger in Neogene than in Quaternary and the strongest East Asia summer monsoon may occur between 4.1 and 4.8 Ma. The relatively small ice volume and high global temperature may be responsible for the strong summer monsoon during the early Pliocene.

A Network Charge-Orineted MOS Transistor Model

The MOS transistor physical model as described in [3] is presented here as a network model. The goal is to obtain an accurate model, suitable for simulation, free from certain problems reported in the literature [13], and conceptually as simple as possible. To achieve this goal the original model had to be extended and modified. The paper presents the derivation of the network model from physical equations, including the corrections which are required for simulation and which compensate for simplifications introduced in the original physical model. Our intrinsic MOS model consists of three nonlinear voltage-controlled capacitors and a dependent current source. The charges of the capacitors and the current of the current source are functions of the voltages $V_{gs}$, $V_{bs}$, and $V_{ds}$. The complete model consists of the intrinsic model plus the parasitics. The apparent simplicity of the model is a result of hiding information in the characteristics of the nonlinear components. The resulted network model has been checked by simulation and analysis. It is shown that the network model is suitable for simulation: It is defined for any value of the voltages; the functions involved are continuous and satisfy Lipschitz conditions with no jumps at region boundaries; Derivatives have been computed symbolically and are available for use by the Newton-Raphson method. The model"s functions can be measured from the terminals. It is also shown that small channel effects can be included in the model. Higher frequency effects can be modeled by using a network consisting of several sections of the basic lumped model. Future plans include a detailed comparison of the network model with models such as SPICE level 3 and a comparison of the multi- section higher frequency model with experiments.

Goizuetako hizkera: adinduen eta L.H.-ko 3. zikloko ikasleen arteko desberdintasunak

[EUS] Bizitza ohiturak aldatzen ari direla begi bistakoa da, eta horrek hizkuntza baten garapenean eragina izaten du. Proiektu honen bitartez, Goizuetako euskalkiak azken berrogeita hamar urte hauetan, gutxi gorabehera, izan duen garapena frogatzen saiatuko naiz. Horretarako, ikerketa bat egin dut gazte eta adinduen artean, euskalkiaren ezaugarrietan eman diren aldakuntzak zeintzuk izan diren ikusteko. Ikerketa honekin hizkuntzaren “transmisioa” nolakoa izan den ikusi ahalko da. Lan honen bidez, euskalkiak eman duen beherakada, hein batean, oraingo biztanleriak kanpo harreman gehiago dituelako eta bizi ohitura berriak hartzen ari delako eman dela ikusten da. Etorkizuneko irakasle bezala, proiektu honen bidez eskualde bakoitzeko hizkuntza garapenaren jakitun izatea ezinbestekoa dela ikusten da.

The operation of multiquantum-well barriers

The multiquantum barrier (MQB), proposed by Iga et al in 1986, has been shown by several researchers to be an effective structure for improving the operating characteristics of laser diodes. These improvements include a reduction in the laser threshold current and increased characteristic temperatures. The operation of the MQB has been described as providing an increased barrier to electron overflow by reflecting high energy electrons trying to escape from the active region of the laser.This is achieved in a manner analogous to a Bragg reflector in optics. This thesis presents an investigation of the effectiveness of the MQB as an electron reflector. Numerical models have been developed for calculating the electron reflection due to MQB. Novel optical and electrical characterisation techniques have been used to try to measure an increase in barrier height due to the MQB in AlGaInP.It has been shown that the inclusion of MQB structures in bulk double heterostructure visible laser diodes can halve the threshold current above room temperature and the characteristic temperature of these lasers can be increased by up to 20K.These improvements are shown to occur in visible laser diodes even with the inclusion of theoretically ineffective MQB structures, hence the observed improvement in the characteristics of the laser diodes described above cannot be uniquely attributed to an increased barrier height due to enhance electron reflection. It is proposed here that the MQB improves the performance of laser diodes by proventing the diffusion of zinc into the active region of the laser. It is also proposed that the trapped zinc in the MQB region of the laser diode locally increases the p-type doping bringing the quasi-Fermi level for holes closer to the valence band edge thus increasing the barrier to electron overflow in the conduction band.

Accounting for Posttraumatic Stress Disorder Symptom Severity With Pre- and Posttrauma Measures: A Longitudinal Study of Older Adults.

Using data from a longitudinal study of community-dwelling older adults, we analyzed the most extensive set of known correlates of PTSD symptoms obtained from a single sample to examine the measures' independent and combined utility in accounting for PTSD symptom severity. Fifteen measures identified as PTSD risk factors in published meta-analyses and 12 theoretically and empirically supported individual difference and health-related measures were included. Individual difference measures assessed after the trauma, including insecure attachment and factors related to the current trauma memory, such as self-rated severity, event centrality, frequency of involuntary recall, and physical reactions to the memory, accounted for symptom severity better than measures of pre-trauma factors. In an analysis restricted to prospective measures assessed before the trauma, the total variance explained decreased from 56% to 16%. Results support a model of PTSD in which characteristics of the current trauma memory promote the development and maintenance of PTSD symptoms.

Macrofauna along the Sudanese Red Sea coast: potential effect of mangrove clearance on community and trophic structure

Mangroves along the Sudanese Red Sea coast are under constant anthropogenic pressure. To better understand the influence of mangrove clearance on the intertidal benthic community, we investigated the composition, biodiversity and standing stock of the macrofauna communities at high-, mid- and low-water levels in three contrasting habitats: a bare sand flat, a cleared mangrove and an intact mangrove. In addition, a community-wide metric approach based on taxon-specific carbon and nitrogen isotope values was used to compare the trophic structure between the three habitats. The habitats differed significantly in terms of macrofaunal standing stock, community composition and trophic structure. The high- and mid-water levels of the intact mangroves showed a distinct macrofaunal community characterized by elevated densities and biomass, largely governed by higher decapod and gastropod abundances. Diversity was similar for cleared and intact mangroves, but much lower for the bare sand flat. Community-wide metrics indicated highest trophic diversity and community niche breadth in the intact mangroves. Differences between the cleared and intact mangroves can be partly attributed to differences in sediment characteristics resulting from mangrove clearance. These results suggest a significant impact of mangrove clearance on the macrofaunal community and trophic structure. This study calls for further investigations and management actions to protect and restore these habitats, and ensure the survival of this ecologically valuable coastal ecosystem.

Passive Control of Plume Interference on Slender Axisymmetric Bodies

The physics of the plume-induced shock and separation, particularly at high plume to exit pressure ratios with and without shock-turbulent boundary-layer control methods, were studied using computational techniques. Mass-averaged Navier-Stokes equations with a two-equation turbulence model were solved by using a fully implicit finite volume scheme and time.marching algorithm. The control methodologies for shock interactions included a porous tail and a porous extension attached at the nozzle exit or trailing edge. The porous tail produced a weaker shock and fixed the shock position on the control surface. The effect of the porous extension on shock interactions was mainly to restrain the plume from strongly underexpanding during a change in flight conditions. These techniques could give an additional dimension to the design and control of supersonic missiles.

Geophysical imaging of stimulated microbial biomineralization

Understanding how microorganisms influence the physical and chemical properties of the subsurface is hindered by our inability to observe microbial dynamics in real time and with high spatial resolution. Here, we investigate the use of noninvasive geophysical methods to monitor biomineralization at the laboratory scale during stimulated sulfate reduction under dynamic flow conditions. Alterations in sediment characteristics resulting from microbe-mediated sulfide mineral precipitation were concomitant with changes in complex resistivity and acoustic wave propagation signatures. The sequestration of zinc and iron in insoluble sulfides led to alterations in the ability of the pore fluid to conduct electrical charge and of the saturated sediments to dissipate acoustic energy. These changes resulted directly from the nucleation, growth, and development of nanoparticulate precipitates along grain surfaces and within the pore space. Scanning and transmission electron microscopy (SEM and TEM) confirmed the sulfides to be associated with cell surfaces, with precipitates ranging from aggregates of individual 3-5 nm nanocrystals to larger assemblages of up to 10-20 m in diameter. Anomalies in the geophysical data reflected the distribution of mineral precipitates and biomass over space and time, with temporal variations in the signals corresponding to changes in the aggregation state of the nanocrystalline sulfides. These results suggest the potential for using geophysical techniques to image certain subsurface biogeochemical processes, such as those accompanying the bioremediation of metal-contaminated aquifers.

SEM-EDX of morphology of electroless nickel coatings with tin and tungsten

Electroless plating of binary Ni-P, ternary Ni-Sn-P and Ni-W-P, and quaternary Ni-W-Sn-P alloy coatings was carried out in alkalicitrate baths. After the plating, several kinds of test were carried out to determine the improvement in the characteristics and properties due to the additional elements as well as to study the change in behaviour when heat treatment was applied to these coatings. The coatings were subjected to X-ray diffraction analysis where it was found that all the coatings were amorphous. Interesting surface morphology features were examined using scanning electron microscopy. Addition of a third element improved the hardness.