Real-time quantitative PCR to determine chlamydial load in men and women in a community setting

We used a PCR method to quantify the loads of Chlamydia trachomatis organisms in self-collected urine and vulvovaginal swab (VVS) samples from 93 women and 30 men participating in the Chlamydia Screening Studies Project, a community-based study of individuals not seeking health care. For women, self-collected VVS had a higher mean chlamydial load (10,405 organisms/ml; 95% confidence interval [95% CI], 5,167 to 21,163 organisms/ml) than did first-void urines (FVU) (503 organisms/ml; 95% CI, 250 to 1,022 organisms/ml; P < 0.001). Chlamydial loads in female and male self-collected FVU specimens were similar (P = 0.634). The mean chlamydial load in FVU specimens decreased with increasing age in females and males. There was no strong statistical evidence of differences in chlamydial load in repeat male and female FVU specimens taken when patients attended for treatment a median of 23.5 (range, 14 to 62) and 28 (range, 13 to 132) days later, respectively, or in VVS taken a median of 35 (range, 14 to 217) days later. In this study, chlamydial load values for infected persons in the community who were not seeking treatment were lower than those published in other studies involving symptomatic patients attending clinical settings. This might have implications for estimates of the infectiousness of chlamydia. The results of this study provide a scientific rationale for preferring VVS to FVU specimens from women.

Real Property, Dunedin, New Zealand, 1896 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: The Survey Districts of North Harbour & Blueskin, Lower Harbour West, North East Valley, Upper Harbour West, Tomahawk, Sawyers Bay, Andersons Bay, Portobello Bay, Otago Peninsula & Upper Harbour East, drawn by G.P. Wilson, April 1896. It was published by N.Z. Lands and Survey in 1896. Covers the Dunedin region, New Zealand. Scale [ca. 1:63,360]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Universal Transverse Mercator (UTM Zone 59S, meters, WGS 1984) projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as property lot and block numbers, boundaries of survey districts and blocks, boroughs, townships and estates, drainage, selected roads, railroads and stations, selected buildings and industry locations, cemeteries, shoreline features, docks and wharves, and more. Relief shown by spot heights.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Intelligent Transportation Systems: real world benefits.

Transportation Department, Joint Program Office for Intelligent Transportation Systems, Washington, D.C.

Crash protection systems for handicapped school and transit bus occupants. Volume III: appendices A, B, C and D. Interim report.

National Highway Traffic Safety Administration, Washington, D.C.

Lessons learned in the D.C. public schools : hearing before the Subcommittee on Oversight of Government Management, Restructuring, and the District of Columbia of the Committee on Governmental Affairs, United States Senate, One Hundred Fifth Congress, second session, March 9, 1998.

Shipping list no.: 98-0336-P.

An advanced real-time ramp metering system (ARMS): the system concept.

Texas Department of Transportation, Austin

Human factors requirements for real-time motorist information displays. Volume 6: questionnaire survey of motorist route selection criteria. Final report.

Federal Highway Administration, Office of Research, Washington, D.C.

Human factors requirements for real-time motorist information displays. Volume 7: analysis of driver requirements for intercity trips. Final report.

Federal Highway Administration, Office of Research, Washington, D.C.

Increased fuel economy in transportation systems by use of energy management. Volume III. Real-time (hybrid computer) simulation program set.

Transportation Department, Washington, D.C.

Review of federal surplus property program, Office of Civil and Defense Mobilization, Executive Office of the President (program transferred to the Department of Defense as of August 1, 1961) : report to the Congress of the United States /

"October 1962."

Treaty provisions relating to the rights of inheritance, acquisitions, and ownership of property in force between the United States and other countries /

Mode of access: Internet.

Payments in lieu of taxes to States of local taxing units. Report of the Committee on Government Operations, United States Senate, Eighty-third Congress, second session, on H.R. 5605, an act of 1949 to provide for payment of taxes or payments in lieu of taxes with respect to real property transferred from Government corporations to other agencies of the Federal Government.

Mode of access: Internet.

Human factors requirements for real-time motorist information displays. Volume 2: state-of-the-art: messages and displays in freeway corridors. Final report.

Federal Highway Administration, Office of Research, Washington, D.C.

Human factors requirements for real-time motorist information displays. Volume 3: summary of systems in the United States. Final report.

Federal Highway Administration, Office of Research, Washington, D.C.

Human factors requirements for real-time motorist information displays. Volume 4: bibliography and selected annotations: visual systems. Final report.

Federal Highway Administration, Office of Research, Washington, D.C.