581 resultados para EMBRIONES VEGETALES


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XI Encuentro Nacional de Dirigentes del Sector Agropecuario. Puerto Plata, República Dominicana 5 a 7 de agosto de 2010

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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This study was carried out to assess the influence of bovine embryo culture medium Beltsville Agriculture Research Center (BARC), supplemented with FCS, BSA or PVA, on the in vitro oocyte maturation, evidenced by cleavage rate and blastocysts production at different developmental stages. Three experiments were performed, as follows: exp.1: addition of FCS to BARC medium at concentrations of 0, 5 and 10%; exp. 2: addition of BSA to BARC medium at concentrations of 0, 4 and 8 mg/ml; exp. 3: addition of PVA to BARC medium at concentrations of 0, 0.5 and 1.0 mg/ml. TCM 199 supplemented with bicarbonate, pyruvate, gentamicin sulfate, FSH, LH and FCS was used as control group. Oocytes obtained from cow ovaries at slaughterhouse were selected in PBS, and then matured in BARC medium supplemented with FSH, LH and gentamicin sulfate, according to the experimental design. Percoll gradient was used for sperm selection and TALP medium for IVF. In vitro embryo culture was in SOF-m medium; a humidified atmosphere with 5% CO2, in air, at 38.7oC was used for all steps. The number of oocytes reaching blastocyst, expanded blastocyst, and hatched blastocyt stages was recorded, respectively at 72 and 168 h post-insemination. ANOVA and Bonferroni t test were used to determine differences among groups. Differences of P<0.05 were taken as significant. Higher percentage (P<0.05) of cleaved oocytes was observed in group TCM + FCS than for the other groups matured in BARC supplemented with FCS or BSA, regardless the concentration used. However, the cleavage rate was similar between groups BARC plus PVA with 1 mg/ml (85.7%) and TCM + FCS (90.8%). Significant difference was found among groups for the production of blastocysts, with the control group yielding a higher number of blastocysts (results ranging from 47.4 to 51.4%, in comparison with groups using BARC + FCS (4.1 to 19.7%), BSA (1.4 to 5.6%) and PVA (5.7 to 10.6%). In conclusion, BARC medium supplemented with different macromolecules did not promote a beneficial effect on in vitro oocyte maturation, resulting in lower rate of cleavage and blastocyst production when compared with TCM + FCS medium.

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Periodontal disease is the result of the interrelationship between microbiotic aggression and the host’s organic defence. Amongst the microorganisms involved in periodontopathies, Fusobacterium nucleatum is conspicuous by establishing a link between the initial and final colonizers, besides producing toxic compounds and adhering to the host’s cells. Control of bacterial biofilm can be achieved by use of chemical agents, many of which extracted from plants. Thus the object of this study was to evaluate the inhibitory activity in vitro of some teas, generally taken in a normal diet, on Fusobacterium nucleatum and your adherence to host’s cells. Minimum inhibitory and bactericidal concentrations were established and haemagglutinative test in microplaques was effected. It was ascertained that all plant extracts have inhibitory activity and that infusions of Camellia sinensis (black tea and green tea), Mentha piperita (mint) and Pimpinella anixem (aniseed) added to the bacteria/erythrocyte compound reduced significantly the adherence of microorganisms.

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The aim of this work was to analyze the acute oral toxicity and the effects on intestinal motility of the extract obtained through decoction 20% (m/v) of Endopleura uchi, popularly known as "uxi-amarelo", a native plant from the Brazilian Amazon. The plant is used indiscriminately against several diseases: arthritis, cholesterol, diabetes, ulcers, myomas, and intestinal illnesses in general, among others. The present results show that there were no significant alterations in intestinal motility and that the extract does not present signs of toxicity, showing its safety for consumption purposes.

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[ES] Una de las limitaciones más importantes para el desarrollo de la acuicultura moderna es la generación de “residuos” tanto sólidos como disueltos que pueden causar problemas de eutrofización. En los denominados sistemas de policultivo integrado (recientemente agrupados bajo el término “Acuicultura Multi-Trófica Integrada – AMTI”) además de diversificar el número de especies animales y vegetales que utilizan los “desechos” producidos por los niveles tróficos superiores, se reducen los efectos causados sobre el medio. En estos sistemas, los nutrientes disueltos (principalmente N-NH y P-PO) son canalizados, como efluentes nutritivos con valor añadido, a través de sistemas para el cultivo de macroalgas en donde son asimilados y reconvertidos en biomasa y sus sustancias bioactivas asociadas, evitando así su dilución y vertido. Las eficiencias de eliminación de estos nutrientes disueltos, las producciones y la calidad de la biomasa obtenidas en el sistema, además de sus posibilidades biotecnológicas, hacen el proceso atractivo desde el punto de vista económico. Las excepcionales condiciones medioambientales del entorno canario han permitido el desarrollo de experiencias en las que el cultivo y la producción de especies de macroalgas de interés aplicado se combina con la mejora de los procesos de biofiltración de aguas residuales producidas en los sistemas intensivos para el cultivo de animales marinos. 

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Máster Oficial en Cultivos Marinos. Trabajo presentado como requisito parcial para la obtención del Título de Máster Oficial en Cultivos Marinos, otorgado por la Universidad de Las Palmas de Gran Canaria (ULPGC), el Instituto Canario de Ciencias Marinas (ICCM), y el Centro Internacional de Altos Estudios Agronómicos Mediterráneos de Zaragoza (CIHEAM)

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[ES] Se confirma la expansión corológica del xenófito, Maireana brevifolia (R.Br.) P.G. Wilson, especie invasora de reciente introducción en Gran Canaria comentándose su ecología y la amenaza que implica para las comunidades vegetales de los espacios naturales protegidos en la isla.

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Máster Universitario International en Acuicultura. Trabajo presentado como requisito parcial para la obtención del Título de Máster Universitario Internacional en Acuicultura, otorgado por la Universidad de Las Palmas de Gran Canaria (ULPGC), el Instituto Canario de Ciencias Marinas (ICCM), y el Centro Internacional de Altos Estudios Agronómicos Mediterráneos de Zaragoza (CIHEAM)