Roles of calcium and IP3 in impaired colon contractility of rats following multiple organ dysfunction syndrome

The purpose of the present study was to explore changes in rat colon motility, and determine the roles of calcium and inositol (1,4,5)-triphosphate (IP3) in colon dysmotility induced by multiple organ dysfunction syndrome (MODS) caused by bacteria peritonitis. The number of stools, the contractility of the muscle strips and the length of smooth muscle cells (SMC) in the colon, the concentration of calcium and IP3 in SMC, and serum nitric oxide were measured. Number of stools, fecal weight, IP3 concentration in SMC and serum nitric oxide concentration were 0.77 ± 0.52 pellets, 2.51 ± 0.39 g, 4.14 ± 2.07 pmol/tube, and 113.95 ± 37.89 µmol/L, respectively, for the MODS group (N = 11) vs 1.54 ± 0.64 pellets, 4.32 ± 0.57 g, 8.19 ± 3.11 pmol/tube, and 37.42 ± 19.56 µmol/L for the control group (N = 20; P < 0.05). After treatment with 0.1 mM acetylcholine and 0.1 M potassium chloride, the maximum contraction stress of smooth muscle strips, the length of SMC and the changes of calcium concentration were 593 ± 81 and 458 ± 69 g/cm³, 48.1 ± 11.8 and 69.2 ± 15.7 µM, 250 ± 70 and 167 ± 48%, respectively, for the control group vs 321 ± 53 and 284 ± 56 g/cm³, 65.1 ± 18.5 and 87.2 ± 23.7 µM, 127 ± 35 and 112 ± 35% for the MODS group (P < 0.05). Thus, colon contractility was decreased in MODS, a result possibly related to reduced calcium concentration and IP3 in SMC.

Endothelium-dependent and -independent hepatic artery vasodilatation is not impaired in a canine model of liver ischemia-reperfusion injury

We investigated whether hepatic artery endothelium may be the earliest site of injury consequent to liver ischemia and reperfusion. Twenty-four heartworm-free mongrel dogs of either sex exposed to liver ischemia/reperfusion in vivo were randomized into four experimental groups (N = 6): a) control, sham-operated dogs, b) dogs subjected to 60 min of ischemia, c) dogs subjected to 30 min of ischemia and 60 min of reperfusion, and d) animals subjected to 45 min of ischemia and 120 min of reperfusion. The nitric oxide endothelium-dependent relaxation of hepatic artery rings contracted with prostaglandin F2a and exposed to increasing concentrations of acetylcholine, calcium ionophore A23187, sodium fluoride, phospholipase-C, poly-L-arginine, isoproterenol, and sodium nitroprusside was evaluated in organ-chamber experiments. Lipid peroxidation was estimated by malondialdehyde activity in liver tissue samples and by blood lactic dehydrogenase (LDH), serum aspartate aminotransferase (AST) and serum alanine aminotransferase (ALT) activities. No changes were observed in hepatic artery relaxation for any agonist tested. The group subjected to 45 min of ischemia and 120 min of reperfusion presented marked increases of serum aminotransferases (ALT = 2989 ± 1056 U/L and AST = 1268 ± 371 U/L; P < 0.01), LDH = 2887 ± 1213 IU/L; P < 0.01) and malondialdehyde in liver samples (0.360 ± 0.020 nmol/mgPT; P < 0.05). Under the experimental conditions utilized, no abnormal changes in hepatic arterial vasoreactivity were observed: endothelium-dependent and independent hepatic artery vasodilation were not impaired in this canine model of ischemia/reperfusion injury. In contrast to other vital organs and in the ischemia/reperfusion injury environment, dysfunction of the main artery endothelium is not the first site of reperfusion injury.

Transplantation and survival of mouse inner ear progenitor/stem cells in the organ of Corti after cochleostomy of hearing-impaired guinea pigs: preliminary results

In mammals, damage to sensory receptor cells (hair cells) of the inner ear results in permanent sensorineural hearing loss. Here, we investigated whether postnatal mouse inner ear progenitor/stem cells (mIESCs) are viable after transplantation into the basal turns of neomycin-injured guinea pig cochleas. We also examined the effects of mIESC transplantation on auditory functions. Eight adult female Cavia porcellus guinea pigs (250-350g) were deafened by intratympanic neomycin delivery. After 7 days, the animals were randomly divided in two groups. The study group (n=4) received transplantation of LacZ-positive mIESCs in culture medium into the scala tympani. The control group (n=4) received culture medium only. At 2 weeks after transplantation, functional analyses were performed by auditory brainstem response measurement, and the animals were sacrificed. The presence of mIESCs was evaluated by immunohistochemistry of sections of the cochlea from the study group. Non-parametric tests were used for statistical analysis of the data. Intratympanic neomycin delivery damaged hair cells and increased auditory thresholds prior to cell transplantation. There were no significant differences between auditory brainstem thresholds before and after transplantation in individual guinea pigs. Some mIESCs were observed in all scalae of the basal turns of the injured cochleas, and a proportion of these cells expressed the hair cell marker myosin VIIa. Some transplanted mIESCs engrafted in the cochlear basilar membrane. Our study demonstrates that transplanted cells survived and engrafted in the organ of Corti after cochleostomy.

Quality assurance of refuse derived fuel with machine vision

This thesis studies the use of machine vision in RDF quality assurance and manufacturing. Currently machine vision is used in recycling and material detection and some commer- cial products are available in the market. In this thesis an on-line machine vision system is proposed for characterizing particle size. The proposed machine vision system is based on the mapping between image segmenta- tion and the ground truth of the particle size. The results shows that the implementation of such machine vision system is feasible.

The Formation Of Internationalization Strategies Within The Construction Industry:Vision and Strategy

Internationalization represents a complex topic that has been researched for quite some time. However, since it continues to be extremely current a topic, its significance has not diminished, but maybe even increased in importance. Companies today face extreme pressure to enter new markets in the hope of growing, becoming more profitable, increasing market share, attracting new customers and meeting the requirements of its share- and stakeholders. In the increasingly global business environment of today, companies are facing both challenges and possible advantages of internationalization. Few companies are not operating internationally and it is becoming the question of ‘Why not?’ rather than ‘Why?’ to internationalize business operations. Internationalization and the importance of strategy are discussed in this research from the viewpoint of three case companies that were interviewed about internationalization strategies. This research project is a qualitative study that answers the research question of How is a business strategy constructed for entering a new market? The sub-questions are • How are goals set and what indicators are used to monitor the achievement of these goals? • What are the key characteristics of a strategy implementation process? The research method chosen for this study is a multiple-case study. Three case companies were chosen for the interviews in order to gain in-depth data of internationalization strategies within the construction industry.

Työnantajabrandin vision suhde todellisuuteen

Kilpailu osaavasta työvoimasta on saanut yritykset panostamaan työnantajabrandiinsa. Työnantajabrandi yhtenä yrityksen brandipääoman osa-alueena auttaa yrityksiä erottumaan kilpailijoista ja siten houkuttelemaan yritykseen sopivia työntekijöitä sekä säilyttämään jo olemassa olevat työntekijät. Työnantajabrandi ei aina näyttäydy yrityksen työntekijöille tai –hakijoille niin kuin yritys sen haluaisi näyttäytyvän. Henkilöstöhallinnon ja johdon visio saattaa siis olla ristiriidassa sen kanssa, miten brandi todellisuudessa koetaan. Mikäli yritys ei pysty viestittämään oikeanlaista brandi-imagoa tai säilyttämään brandilupauksiaan vaikuttaa tämä yrityksen työntekijöiden sitoutumiseen ja tyytyväisyyteen negatiivisesti. Lisäksi yritykseen hakeutuu töihin yrityksen arvomaailmaan sopimattomia työntekijöitä. Tutkimuksen tarkoituksena on selvittää työnantajabrandin vision suhdetta todellisuuteen case-yrityksen kautta. Case-yritykseksi on valittu kansainvälinen konsultointiyritys, jolle osaava työvoima on elinehto. Tutkimusmenetelmäksi valittiin puolistrukturoidut haastattelut eli aihetta on lähestytty laadullisen menetelmän kautta. Tutkimukseen haastateltiin vision selvittämiseksi case-yrityksen henkilöstöhallintoa sekä perehdyttiin olemassa olevaan materiaaliin yrityksen brandista. Vision toteutumisen selvittämiseksi haastateltiin 19 työntekijää yrityksen kahdesta eri liiketoimintayksiköstä. Teorian pohjalta tarkentunut teoreettinen viitekehys pitää sisällään yrityksen psykologisen sopimuksen, sisäisen ja ulkoisen brandiviestinnän sekä organisaatiokulttuurin. Haastattelut rakennettiin näiden teemojen ympärille. Empiirisen osuuden tulokset vahvistavat teoreettista viitekehystä, jonka mukaan psykologisella sopimuksella, brandiviestinnällä ja organisaatiokulttuurilla on vaikutusta siihen, miten työnantajabrandi koetaan yrityksen työntekijöiden keskuudessa. Organisaatiokulttuurin ja sitä ilmentävän brandipersoonallisuuden osalta korostuu yrityksen työntekijöiden sopivuus ja yhtäläisyys yrityksen organisaatiokulttuuriin voimistamaan haluttua brandimielikuvaa. Psykologisen sopimuksen osalta empiria tukee todenmukaisen kuvan luomisen merkitystä yrityksen brandiviestinnässä. Lisäksi psykologisen sopimuksen osalta sosiaaliset edut nähdään tärkeänä tekijänä työnantajassa. Ihmisistä välittämisellä ja työilmapiirillä voidaan nähdä olevan suuri merkitys työntekijöiden työssä viihtymiselle. Työntekijät myös odottavat nykypäivänä nopeaa urakehitystä ja koulutukseen panostamista uraltaan. Lisäksi joustavuus ja vapaa-ajan ja työn tasapainottaminen koetaan tärkeiksi. Haastattelut tukevat myös teoreettisen viitekehyksen oletusta, että yrityksen visio työnantajabrandista eroaa usein siitä kuvasta, joka työntekijöille välittyy

Design and implementation of machine vision system for the mobile assembly robot

The review of intelligent machines shows that the demand for new ways of helping people in perception of the real world is becoming higher and higher every year. This thesis provides information about design and implementation of machine vision for mobile assembly robot. The work has been done as a part of LUT project in Laboratory of Intelligent Machines. The aim of this work is to create a working vision system. The qualitative and quantitative research were done to complete this task. In the first part, the author presents the theoretical background of such things as digital camera work principles, wireless transmission basics, creation of live stream, methods used for pattern recognition. Formulas, dependencies and previous research related to the topic are shown. In the second part, the equipment used for the project is described. There is information about the brands, models, capabilities and also requirements needed for implementation. Although, the author gives a description of LabVIEW software, its add-ons and OpenCV which are used in the project. Furthermore, one can find results in further section of considered thesis. They mainly represented by screenshots from cameras, working station and photos of the system. The key result of this thesis is vision system created for the needs of mobile assembly robot. Therefore, it is possible to see graphically what was done on examples. Future research in this field includes optimization of the pattern recognition algorithm. This will give less response time for recognizing objects. Presented by author system can be used also for further activities which include artificial intelligence usage.