Testicular FasL is expressed by sperm cells

The testis is the main source of Fas ligand (FasL) mRNA in rodents; it is generally believed that this molecule, expressed on bordering somatic Sertoli cells, bestows an immune-privileged status in the testis by eliminating infiltrating inflammatory Fas-bearing leukocytes. Our results demonstrate that the attribution of testicular expression of FasL to Sertoli cells is erroneous and that FasL transcription instead occurs in meiotic and postmeiotic germ cells, whereas the protein is only displayed on mature spermatozoa. These findings point to a significant role of the Fas system in the biology of mammalian reproduction.

Cofilin Phosphorylation by Protein Kinase Testicular Protein Kinase 1 and Its Role in Integrin-mediated Actin Reorganization and Focal Adhesion Formation

Testicular protein kinase 1 (TESK1) is a serine/threonine kinase with a structure composed of a kinase domain related to those of LIM-kinases and a unique C-terminal proline-rich domain. Like LIM-kinases, TESK1 phosphorylated cofilin specifically at Ser-3, both in vitro and in vivo. When expressed in HeLa cells, TESK1 stimulated the formation of actin stress fibers and focal adhesions. In contrast to LIM-kinases, the kinase activity of TESK1 was not enhanced by Rho-associated kinase (ROCK) or p21-activated kinase, indicating that TESK1 is not their downstream effector. Both the kinase activity of TESK1 and the level of cofilin phosphorylation increased by plating cells on fibronectin. Y-27632, a specific inhibitor of ROCK, inhibited LIM-kinase-induced cofilin phosphorylation but did not affect fibronectin-induced or TESK1-induced cofilin phosphorylation in HeLa cells. Expression of a kinase-negative TESK1 suppressed cofilin phosphorylation and formation of stress fibers and focal adhesions induced in cells plated on fibronectin. These results suggest that TESK1 functions downstream of integrins and plays a key role in integrin-mediated actin reorganization, presumably through phosphorylating and inactivating cofilin. We propose that TESK1 and LIM-kinases commonly phosphorylate cofilin but are regulated in different ways and play distinct roles in actin reorganization in living cells.

Loss of sequences 3' to the testis-determining gene, SRY, including the Y pseudoautosomal boundary associated with partial testicular determination.

The condition termed 46,XY complete gonadal dysgenesis is characterized by a completely female phenotype and streak gonads. In contrast, subjects with 46,XY partial gonadal dysgenesis and those with embryonic testicular regression sequence usually present ambiguous genitalia and a mix of Müllerian and Wolffian structures. In 46,XY partial gonadal dysgenesis gonadal histology shows evidence of incomplete testis determination. In 46,XY embryonic testicular regression sequence there is lack of gonadal tissue on both sides. Various lines of evidence suggest that embryonic testicular regression sequence is a variant form of 46,XY gonadal dysgenesis. The sex-determining region Y chromosome gene (SRY) encodes sequences for the testis-determining factor. To date germ-line mutations in SRY have been reported in approximately 20% of subjects with 46,XY complete gonadal dysgenesis. However, no germ-line mutations of SRY have been reported in subjects with the partial forms. We studied 20 subjects who presented either 46,XY partial gonadal dysgenesis or 46,XY embryonic testicular regression sequence. We examined the SRY gene and the minimum region of Y-specific DNA known to confer a male phenotype. The SRY-open reading frame (ORF) was normal in all subjects. However a de novo interstitial deletion 3' to the SRY-ORF was found in one subject. Although it is possible that the deletion was unrelated to the subject's phenotype, we propose that the deletion was responsible for the abnormal gonadal development by diminishing expression of SRY. We suggest that the deletion resulted either in the loss of sequences necessary for normal SRY expression or in a position effect that altered SRY expression. This case provides further evidence that deletions of the Y chromosome outside the SRY-ORF can result in either complete or incomplete sex reversal.

Functional testicular tissue does not masculinize development of the zebra finch song system.

Current theories of sexual differentiation maintain that ovarian estrogen prevents masculine development of the copulatory system in birds, whereas estrogen derived from testicular androgens promotes masculine sexual differentiation of neuroanatomy and sexual behavior in mammals. Paradoxically, some data suggest that the neural song system in zebra finches follows the mammalian pattern with estrogenic metabolites of testicular secretions causing masculine development. To test whether the removal of estrogen from males during early development would prevent the development of masculine song systems, zebra finches were treated embryonically with an inhibitor of estrogen synthesis. In addition, this treatment in genetic female zebra finches induced both functional ovarian and testicular tissue to develop, thus allowing the assessment of the direct effects of testicular secretions on song system development. In males, the inhibition of estrogen synthesis before hatching had a small but significant effect in demasculinizing one aspect of the neural song system. In treated females, the song systems remained morphologically feminine. These results suggest that masculinization of the song system is not determined solely by testicular androgens or their estrogenic metabolites.

Fashion films as a new communication format to build fashion brands

The consolidation of collaborative video platforms such as YouTube and Vimeo in recent years has significantly changed the way fashion brands communicate with their audiences. Fashion films have emerged as a new and revolutionary tool adopted by luxury brands at the start of the XXI Century to construct their brands. A sample of 62 fashion films from 2006 to 2016 was analyzed in order to describe fashion film’s anatomy and its main characteristics that constitute an especial type of branded content, originated by brands in their quest for exclusivity and authenticity. As a distinctive type of experiential marketing mostly used by luxury fashion brands, they would become a new communication strategy for mainstream brands, but also allow the discovery of a profound connection with consumers through audiovisual narration.

46,XX doença do desenvolvimento sexual testicular : a propósito de um caso clínico

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Traduzione audiovisiva e format gastronomici: proposta di sottotitolaggio in italiano e in francese della finale di Masterchef

In questo mio elaborato ho affrontato il tema della traduzione audiovisiva. Mi sono soffermata sulla tecnica del sottotitolaggio, concentrando la mia attenzione sui format televisivi di cucina. Sono stata spinta da una vera e propria passione per tutto ciò che riguarda la sfera gastronomica e i programmi televisivi a questa dedicati. Oggi queste trasmissioni hanno raggiunto un vero e proprio successo mondiale. La nostra è l’era dei grandi format televisivi che non parlano altro che di cucina, sfide tra i fornelli e critiche spietate. La cucina è alla base di ogni civiltà ed è parte integrante della cultura di ogni popolo. Tra le molteplici sfaccettature delle culture, troviamo la gastronomia che, dal mio punta di vista, è situata ai primi posti per importanza. L’Italia e la Francia hanno, sin da sempre, dimostrato la loro eccellenza all’interno del panorama mondiale enogastronomico: la cucina italiana, con la sua semplicità e i suoi sapori mediterranei, e quella francese, ricca di note tradizionali ed esotiche all’unisono. Per questo motivo, la mia attenzione si è rivolta a un talk show culinario ormai noto in tutto il mondo: MasterChef. In questo format televisivo, i concorrenti sono tenuti a sostenere varie prove, nelle quali bisogna realizzare piatti dell’arte culinaria del paese in cui il programma viene trasmesso e non solo. Scopo del programma è aggiudicarsi il titolo di miglior chef. Ho scelto chiaramente un’edizione a mio piacimento del programma per entrambi i paesi e ho proposto un sottotitolaggio per un particolare momento di questo format televisivo: la finale. Nella fattispecie, ho proposto il sottotitolaggio per la finale di MasterChef Italia in francese e per la finale di MasterChef France in italiano. Ho scelto di sottotitolare questo particolare momento della trasmissione televisiva, in quanto ritengo che, oltre ad essere uno dei più emozionanti per i telespettatori, sia ricco di elementi utili per un’analisi approfondita in ambito traduttivo.

Microsatellite genotypic data of Euptelea pleiospermum in STRUCTURE format

We sampled leaves from 678 individuals in 21 natural populations (30-36 individuals per population), covering the entire distribution of Euptelea pleiospermum in China.Total DNA was isolated from about 50 mg powdered leaf tissue following the protocol of a DNA extraction kit (Tiangen Biotech Co., LTD., Beijing, China). We used seven fluorescence-labeled microsatellite loci (EP036, EP059, EP081, EP087, EP091, EP278 and EP294; Zhang et al., 2008) to genotype our 678 DNA samples.